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Gene Section CREB3L2  (cAMP  responsive  element  binding
Gene Section CREB3L2 (cAMP responsive element binding

... RRKKKEY which is exactly conserved among CREB, CREM, ATF1, ATF6 and CREBL1. The leucine zipper motif of CREB3L2 is similar to that in CREB3L3 and CREB3L4 (pattern L-X6-C-X6-L-X6-L-X6-L-X6-L). It contains six repeats and consists of five leucines and one cysteine at the second heptad position (amino ...
Bio102A organic notes (2)
Bio102A organic notes (2)

... insulation & protection ...
No Slide Title
No Slide Title

... • use intensifying screens for high energy isotopes • use fluors impregnated in gel for low and medium energy isotopes ...
Test #4: Biomolecule Foldable
Test #4: Biomolecule Foldable

... subunits called nucleotides has which of these functions in the cell?  F ...
Gene Expression
Gene Expression

... • Proteins (enzymes) can be used to make all the other molecules a cell needs: carbohydrates, lipids and nucleic acids. • A segment of DNA that carries the instructions to make (codes for) a protein is called a gene. ...
Lecture_9
Lecture_9

... Isoelectric focusing allows separation of proteins in a gel on the basis of their relative amounts of acidic and basic amino acids. If a mixture of proteins is placed in a gel with a pH gradient and an electrical field is applied, proteins will migrate to their isoelectric point, the pH at which ...
Polymerase Chain Reaction and DNA Sequencing
Polymerase Chain Reaction and DNA Sequencing

... A colour reaction can be seen on specific media. You can use this to – Study the interaction between two proteins which you expect to interact – Find proteins (prey) which interact with a protein you have already (bait). ...
How Much Protein Do You Need
How Much Protein Do You Need

DNA to Protein Synthesis
DNA to Protein Synthesis

... tRNA complexes bind to mRNA codon by forming complementary base pairs with the tRNA anticodon The ribosome moves from codon to codon along the mRNA. Amino acids are added one by one ...
slides
slides

英語(PDF)
英語(PDF)

... screening, modification of natural substances and biologic-based approaches. To investigate whether some strategies have been more successful than others in the discovery of new drugs, we analysed the discovery strategies and the molecular mechanism of action (MMOA) for new molecular entities and ne ...
DNA Transcription & Protein Translation
DNA Transcription & Protein Translation

... complimentary tRNA base triplet (called an anticodon). 4. Each tRNA is specific to an amino acid, as tRNA's are added to the sequence, amino acids are linked together by peptide bonds, eventually forming a protein that is later released by the tRNA. 5. Codon code is specific, but redundant!  20 ami ...
Transformation and Cloning
Transformation and Cloning

... • Remember that a gene is a piece of DNA. • It provides the instructions (codes) for a protein that gives an organism a particular trait. Genetic transformation • This is the uptake of naked DNA from the environment. • Can induce this to happen in a laboratory situation – inserting the DNA you want ...
Cell Membrane Structure & Function
Cell Membrane Structure & Function

... – 2.Take up molecules present in high concentration – 3 Part of protein extends through bilayer – 4.May be non polar helix beta-pleated sheets of non polar amino acids – 5.Non polar portion held within interior of bilayer – 6.Polar ends protrude from both sides of membrane B. Enzymes – 1. Chemical r ...
Biochemistry WebQuest
Biochemistry WebQuest

see examples of typical exams - IQ-USP
see examples of typical exams - IQ-USP

... number of technological advances, in which some new and unpublished techniques wee combined with other well established ones. The first step was to determine the genome of the “mother” bacteria (Mycoplasma mycoideum). a. Describe in detail a technique used to sequence DNA. After the DNA fully sequen ...
Document
Document

... Amino acids are linked by peptide bonds – a form of covalent bond. ...
3 - University High School
3 - University High School

... Interesting Scientific Fact: Proteins control almost everything that happens inside of a living organism. However, proteins are easily destroyed, or denatured. When they are denatured, they no longer work properly. They can be denatured by extreme temperatures and pH. That is why it is important th ...
Beta sheets are twisted
Beta sheets are twisted

... C4 zinc finger: Its consensus sequence is CX2CX13CX2CX1415CX5CX9CX2C. The first four cysteine residues bind to a zinc ion and the last four cysteine residues bind to another zinc ion C6 zinc finger. It has the consensus sequence CX2CX6CX56CX2CX6C. The yeast's Gal4 contains such a motif where six cys ...
Brown eyes, blue eyes. From a gene to its protein
Brown eyes, blue eyes. From a gene to its protein

... Deep
View
is
an
application
that
provides
a
user
friendly
interface
allowing
to
view
protein
 3D
 structures,
 create
 models
 and
 also
 analyse
 several
 proteins
 at
 the
 same
 time.
 The
 proteins
can
be
superimposed
in
order
to
deduce
structural
alignments
and
compare
their
 active
 sites
 or
 ...
Structural Biology in the Pharmaceutical Industry
Structural Biology in the Pharmaceutical Industry

... putative target indeed results in the expected effects in cellular assays (e.g. slowing down the proliferation rate of cancer cell lines, while not affecting non-tumor cell lines). Even at this early stage, long before a decision has been made to clone, express and crystallize this protein, Structur ...
Key: Biomolecule Study Guide 1) In animals, excess carbohydrates
Key: Biomolecule Study Guide 1) In animals, excess carbohydrates

... Key: Biomolecule Study Guide ...
Chapter 2 - FacultyWeb
Chapter 2 - FacultyWeb

... It is an essential component of eicosanoids It is the only site of storage for lipid soluble vitamins. ...
Proteins & Nucleic Acids - St. Mary Catholic Secondary School
Proteins & Nucleic Acids - St. Mary Catholic Secondary School

... Proteins are the “working” molecules of the body. They can act as enzymes, aiding in chemical reactions, or be structural components such as fingernails. Proteins have a very specific 3-D shape which relates directly with their function – if this shape is not exact in every way, the protein may not ...
Chapter 13
Chapter 13

... Protein Synthesis The Genetic Code: 1. The sequence of the DNA bases “codes” for the individual amino acids in a protein. 2. This code is copied on to an mRNA strand. 3. The mRNA code: - 3 mRNA bases in a row are called a ___________________ & each codes for a particular amino acid. 4. Because there ...
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Two-hybrid screening



Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.
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