• Study Resource
  • Explore Categories
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
Chapter 15 Review Questions
Chapter 15 Review Questions

... a protein is its amino acid chain, bonded together with peptide bonds (amide linkages). The secondary structure of a protein begins to shape the amino acid chain using hydrogen bonding, forming alpha-helix and beta-pleated sheet structures. The tertiary structure of a protein gives it 3 dimensions. ...
Lecture 2 Slides
Lecture 2 Slides

... uncharged polar amino acids can H-bond!!! –Ser-OH, Thr-OH and Tyr-OH with for example Glu=O or Gln=O) • Covalent bonds (disulfide bonds between cysteines, see Fig. 4-29) • Hydrophobic interactions-non-polar side chains associate in the interior of the molecule and exclude water) (Fig. 4-5) • van der ...
BOXIN – AN ICHTHYOTOXIC PROTEIN FROM BOXFISHES  Research Article
BOXIN – AN ICHTHYOTOXIC PROTEIN FROM BOXFISHES Research Article

... chromatography and by the acetone-extraction pellet containing active proteins [6]. An intriguing query which arises here is that whether the protein fractions from boxfish secretions are amphipathic surfactant polypeptides alike pardaxin or grammistin isolated from skin secretions of flatfish and s ...
A1985ASW1100001
A1985ASW1100001

... 1 adenylates and inorganic pyrophosphate. Zamecnik’s group had already shown that this same fraction was required for incorporation of amino acids into peptide linkages in protein in the presence of microsomes, ATP, and GTP (reviewed in reference 2). At this time there was no evidence as to how amin ...
MacromoleculeReview
MacromoleculeReview

... 23. A peptide bond is always formed between the ______________________ group of one _________________________ and the ______________________ group of the next. 24. Using a structural formula diagram, show how a peptide bond is formed between two amino acids. ...
Final Exam: Multiple Choice Portion Biochem Block Spring 2016
Final Exam: Multiple Choice Portion Biochem Block Spring 2016

... more questions later today so please check back... *** You are allowed to use a calculator for this exam. ***You may use any of the handouts and your main lesson book for this exam. Nothing else. However, you can email/text/call me if you have any questions. My email is [email protected]; my cell i ...
Protein Structure - Chemistry Courses: About: Department
Protein Structure - Chemistry Courses: About: Department

... 1. Which statement is false about a globular protein that performs its biological function as a single independent polypeptide chain? A) Its tertiary structure is likely stabilized by the interactions of amino acid side chains in non-neighboring regions of the polypeptide chain. B) It could contain ...
Protein Synthesis
Protein Synthesis

... • Proteins are made in the cytoplasm of cells by organelles called ribosomes • Ribosomes may be free in the cytosol or attached to the surface of rough ER ...
Chapter 7 Review
Chapter 7 Review

... rRNA. During treatment B the fluorescent label would only be localized in RNA structures still active in the cell, for example, mRNA, rRNA, and tRNA, and will not be freely distributed in the cytosol or the nucleus. 69. When tetracycline associates with the ribosome and prevents the aminoacyl-tRNA f ...
DNA Transcription and Translation
DNA Transcription and Translation

... 3. Identify which step in transcription would be affected and predict what would happen in each situation: (a) The termination sequence of a gene is removed. ...
S9. Computational Molecular Modeling
S9. Computational Molecular Modeling

... knowledge of how deletions of DNA base pairs in the DNA result in frameshift mutations at the protein level. If the instructor has not yet covered frameshift mutations in class it is probably best to complete this activity during the week 4 laboratory session rather than assign it as homework. Even ...
Organic Molecules - University of Dayton
Organic Molecules - University of Dayton

... Organic (Food) Molecules ...
Bioinformatics
Bioinformatics

... • Transcription and translation are the two main processes linking gene to protein • Genes provide the instructions for making specific proteins. • The bridge between DNA and protein synthesis is RNA. • RNA is chemically similar to DNA, except that it contains ribose as its sugar and substitutes the ...
Eukaryotic protein kinases and choline kinases share a common
Eukaryotic protein kinases and choline kinases share a common

... emergence of nucleated cells in the third superkingdom of life. Choline kinases (ChKs) could also be important in the early evolution of eukaryotes, because of their function in the biosynthesis of phosphatidylcholine, which is unique to eukaryotic membranes. However, the genomic origins of ePKs and ...
Uncommon amino acids, amino acids forming proteins
Uncommon amino acids, amino acids forming proteins

... are least soluble in water and can be precipitated from the solution ...
charged
charged

... (several codes can code for the same amino acid),it is non-overlapping and comma free. One of codons of methionine (ATG=AUG) serves as start signal, but the stop codons code no amino acids. ...
Protein Supplements
Protein Supplements

...  Workout supplement can cause heart failure in individuals with prior cardiovascular disease or if overused or abused. Always consult physician before using!  Workout supplements will automatically build muscle?  Workout supplements will not aid in muscle growth unless paired with regular exercis ...
Information Sheet - HJ Baker & Bro., Inc.
Information Sheet - HJ Baker & Bro., Inc.

... Every farm is different. Different cows, different management practices, different forages, different temperature zones ... different. For many of these farms H.J. Baker’s original PRO-LAK® By-pass Protein Concentrate - backed with over 20 years of research - is just what they need. The approach and ...
Hands-on session on Bioinformatics
Hands-on session on Bioinformatics

... intermolecular interactions, and allosteric modifications ...
AP Biology
AP Biology

... 14. Draw two amino acids – note the amino group, the carboxyl group and the alpha carbon, circle the water molecule to be removed and then note the peptide bond formed when the two are joined. ...
protein - Portal UniMAP
protein - Portal UniMAP

... Strong acid or base – changes in pH result in protonation of some protein side group, which alter/disrupt hydrogen bonding & salt bridge. As a protein approaches its isoelectric point, it becomes insoluble and precipitates from solution. Organic solvents – water-soluble organic solvents eg. Ethanol ...
week 10_protein
week 10_protein

... Strong acid or base – changes in pH result in protonation of some protein side group, which alter/disrupt hydrogen bonding & salt bridge. As a protein approaches its isoelectric point, it becomes insoluble and precipitates from solution. Organic solvents – water-soluble organic solvents eg. Ethanol ...
Globular Protein Structure
Globular Protein Structure

... domain whereas larger proteins (remember proteins can have molecular weights of > 1 x 106) can have as many as several dozen. The term domain is very often used (carelessly) when speaking about protein structure. Therefore it is important to understand the different uses of this fundamental unit of ...
PROTEINS – STRUCTURE AND FUNCTION (DR. TRAISH)
PROTEINS – STRUCTURE AND FUNCTION (DR. TRAISH)

... 2. hydrogen bonding, hydrophobic interactions, electrostatic interactions, van der Waals’ forces all stabilize conformation 3. For many proteins, tertiary is the highest level of structure iv. Quaternary Structure 1. When there is more than one subunit (even if they are all the same) 2. Structure fo ...
Télécharger la version pdf
Télécharger la version pdf

... sprout new leaves in the spring and shed them in the fall. No longer needed, damaged or dead organs such as blossoms and leaves are also cast off by a process known as abscission. By doing so, plants conserve energy and prepare for the next step in their life cycle. But how does a plant know when it ...
< 1 ... 372 373 374 375 376 377 378 379 380 ... 466 >

Two-hybrid screening



Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.
  • studyres.com © 2026
  • DMCA
  • Privacy
  • Terms
  • Report