• Study Resource
  • Explore Categories
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
Proteins
Proteins

... • Denaturation does not break the primary structure (it just unravels the tertiary structure) • Denaturation of an egg with a strong acid video ...
INTEIN MEDIATED PROTEIN SPLICING
INTEIN MEDIATED PROTEIN SPLICING

... X-XbaI,B-BamHI,E-EcoRV,H-HindIII,K-KpnI,N-NacI,P-PstI,S-SacII,Sp-SphI ...
Supplementary Figure 1
Supplementary Figure 1

... To obtain a more quantitative perspective on the sequence relationships between ubiquitin variants of different species, we used this phylogram to compare the protein sequences of UbS27a with the ribosomal S27a domain and with homologs to the ubiquitinlike modifier SUMO1 as a reference. The UPGMA al ...
PostDoc position at the Division of Cell Biology @ Biocenter
PostDoc position at the Division of Cell Biology @ Biocenter

3.27.12 lecture protein
3.27.12 lecture protein

... cells – Uses ATP Because of N recycling this reaction may not be that important ...
List of topics - bio.utexas.edu
List of topics - bio.utexas.edu

... Step 1: Catalase and most other peroxisomal matrix proteins contain a C-terminal PTS1 uptake-targeting sequence (-Ser-Lys-Leu-COO¯ or a related sequence) that binds to the cytosolic receptor Pex5. PTS2 is an amino-terminal targeting sequence contained in a protein leader sequence, which is cleaved ( ...
Life Sciences 1a Practice Problems 6
Life Sciences 1a Practice Problems 6

... c) 2664 nucleotides not including the stop codon. If they include the stop codon (2667) it is fine. It is also okay if they add three for the start codon (2670) and say this methionine is sometimes cleaved off. d) Yes, there is a disparity. The start site of transcription is not the same as the star ...
RNA and Protein Synthesis
RNA and Protein Synthesis

... 2. Frameshift mutation- insertion or deletion of nucleotide. It causes big changes because it can alter protein shape by making it unable to perform normal functions. Example: Tay-Sachs Disease Children with Tay-Sachs, a progressive neurodegenerative disease that attacks nerve cells, usually die be ...
Modes of Macromolecular Classification
Modes of Macromolecular Classification

... links between individual amino acids) and think of a protein’s three-dimensional structure as simply the relative location of individual amino acids (in the manner we think of a crystalline structure). This approach, however, fails to accommodate the movement of a protein’s components. The whole fol ...
Class details
Class details

PROTEIN ANALYSIS - Farmasi Carbon 2012
PROTEIN ANALYSIS - Farmasi Carbon 2012

... Most proteins exhibit strong UV light absorption at 280 nm because they contain “chromophoric” side chains such as tyrosine, tryptophan, and phenylalanine. ...
File
File

... DNA gets all the glory, Proteins do all the work ...
Proteins
Proteins

Essential Nutrients Overview Handout
Essential Nutrients Overview Handout

Chapter 3 Exam
Chapter 3 Exam

... 6. Which diagram and picture in the figure below would best represent the results obtained when placing red blood cells into a hypertonic solution? ...
Pre-lab 8: Peptides and Proteins
Pre-lab 8: Peptides and Proteins

Stage proposé par « Prénom NOM
Stage proposé par « Prénom NOM

... differentiation are under translational control. Such proteins are expressed at very specific steps in elongating spermatids. We have recently shown that Poly(A) Binding Protein (PABP) interacting protein 2 (Paip2) is a major translational regulator involved in the maturation of male germ cells and ...
The postsynaptic NMDA-receptor–PSD-95
The postsynaptic NMDA-receptor–PSD-95

... an abundant PSD protein that forms a two-dimensional lattice immediately under the postsynaptic membrane. The PDZ domains of PSD-95 also bind to other postsynaptic membrane proteins, including potassium channels (K+ Ch), tyrosine kinases (ErbB4) and cell adhesion molecules (neuroligin). Having multi ...
Gene expression PPT
Gene expression PPT

... Transfer RNA (tRNA) transports and positions amino acids. ...
DNA Protein synthesis Review Answer Key.doc
DNA Protein synthesis Review Answer Key.doc

...  The first step in making a protein is to make a copy of the ___________ in the nucleus. DNA or Gene  What nucleic acid contains the master code for making proteins? DNA  What nucleic acids acts as a blueprint in copying the master code? mRNA  Compare and contrast the nitrogen bases on DNA and R ...
Biology Common Assessment Name
Biology Common Assessment Name

... c. tree d. rabbit ...
Final Exam from S06
Final Exam from S06

... b. Name one type of non-coding DNA that is transcribed. Use proper term____________________ No explanation necessary. c. Briefly explain the last sentence in the legend to this figure by explaining how non-coding sequences could increase an organism’s complexity. 2-3 sentences using proper terminolo ...
Chapter 11 - Evangel University
Chapter 11 - Evangel University

... • _________________________ domains - rich in Asp and Glu. Gal4 has domain of 49 amino acids, 11 are acidic • _________________________ domains - Seen in several transcription factors. Sp1 has 2 glutaminerich domains, one with 39 Glu in 143 amino acids • _________________________ domains - Seen in C ...
Amino acids
Amino acids

... and van der Waals interactions among hydrophobic R groups. – While these three interactions are relatively weak, strong covalent bonds called disulfide bridges that form between the sulfhydryl groups (SH) of two cysteine monomers act to rivet parts of the protein together. ...
Animal Digestion
Animal Digestion

... Animals with more than one stomach  Chew their cud (re-chew food)  Cows, sheep, goats  Cows have four stomachs  Alf has eight stomachs ...
< 1 ... 364 365 366 367 368 369 370 371 372 ... 466 >

Two-hybrid screening



Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.
  • studyres.com © 2026
  • DMCA
  • Privacy
  • Terms
  • Report