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Ch 2 - Biochemistry
Ch 2 - Biochemistry

Chapter 3
Chapter 3

... number of hydrogen atoms cannot bond to the carbons at the double bond – These compounds are called unsaturated fats because they have fewer than the maximum number of hydrogens – Fats with the maximum number of hydrogens are called saturated fats ...
PPT
PPT

... Salt bridges – ionic bonds form between acidic and basic residues Hydrogen bonds – form between polar residues ...
PAPER 1 1.Which pair of features is correct for both plant and
PAPER 1 1.Which pair of features is correct for both plant and

... Two chromosomes with differing sets of genes, in the same sequence, with the same alleles B. Two chromosomes with the same set of genes, in a different sequence, with the same alleles C. Two chromosomes with a different set of genes, in the same sequence, with different alleles D. Two chromosomes wi ...
PROTEINS The
PROTEINS The

... This receptor-G-protein complex is linked to and activates phospholipase C, leading to an increase in IP3 and DAG, which work together to activate enzymes and to increase intracellular calcium levels. ...
In Silico Analysis: Annotations about Structural and Functional
In Silico Analysis: Annotations about Structural and Functional

... contains structural and functional annotations, cross-database links and citation references for each group. For each Pfam family there have two multiple alignments: (1) the seed alignments which contains a little number of representative members, and (2) the full alignment containing all members in ...
Macromolecules: Building blocks of life
Macromolecules: Building blocks of life

... processes, because molecules and ions must be free to move and interact, which only happens when they are dissolved in water. Water also transports materials in living organisms, such as in blood or sap. ...
Protein Folding
Protein Folding

... problem is that as proteins are synthesised hydrophobic regions must not be exposed to an aqueous environment or they will associate to form aggregates. This ...
Transcription and Translation
Transcription and Translation

... the aa’s in • Example – read sentence one letter off • Remove one, two or three bases… • Only by removing three bases is the reading frame unchanged A: Therefore, a codon must be three bases. ...
Transcription and Translation
Transcription and Translation

... the aa’s in • Example – read sentence one letter off • Remove one, two or three bases… • Only by removing three bases is the reading frame unchanged A: Therefore, a codon must be three bases. ...
Transcription and translation
Transcription and translation

... • EPO boosts production of red blood cells – Lance Armstrong used it. • Concern now that athletes may inject genes to make EPO into their cells • New test can scan for this gene using introns/exons! • A person’s own EPO gene has introns. • An inserted gene would likely lack those introns. So their a ...
Chapter 4 - Organic Chemistry, Biochemistry
Chapter 4 - Organic Chemistry, Biochemistry

Chapter 3 Amino Acids, Peptides, Proteins
Chapter 3 Amino Acids, Peptides, Proteins

... can double check results against MW to be sure you have everything Note - this only tells you overall % composition, NOT sequence. For that you need to dig harder C. Sequence Determination i. (a start) Figure 3-25 Take protein and modify with FDNB 2,4-dinitrofluorobenzene also called Sanger reagent ...
Lecture-1-molbio
Lecture-1-molbio

... • In eukaryotic cells: – Primary messenger RNA is processed to create mature mRNA – this processing involves splicing out certain segments of the RNA called introns – mature mRNA then transported out of the nucleus • Mature mRNA is translated into protein – by a ribosome ...
WHAT THEY DO
WHAT THEY DO

... If the train is the whole polymer, what would be the small groups that make up the train? If the necklace is the polymer, what are the monomers that make up the necklace? ...
chapter 5 the structure & function of macromolecules
chapter 5 the structure & function of macromolecules

...  Link together by peptide bonds to form a polypeptide chain  Only 20 amino acids ...
Regulation of Gene Expression
Regulation of Gene Expression

... The availability of sigma factors can be used to regulate sets of genes. For example, a group of genes whose product is rarely needed might have a different promoter sequence than other genes and thus require different sigma factors. These genes would only be transcribed when the correct sigma facto ...
Protein_structure_I
Protein_structure_I

... • Two or more independent tertiary structures are assembled into a larger protein complex. • Important for understanding protein-protein interactions. ...
Product PDF for Catalog Number: 103-M452
Product PDF for Catalog Number: 103-M452

... does not share aa sequence homology with any of the “short” pentaxins. Unlike CRP and SAP, which forms pentamers only, TSG14 forms both pentameric and higher ordered oligomers. Similar to CRP and SAP, TSG14 binds to the complement cascade component C1q. However, TSG14 does not bind to phosphoethanol ...
Topology of membrane protein
Topology of membrane protein

... Doyle et al. (1998) Science 280 : 69 - 77 ...
Protein Synthesis
Protein Synthesis

... Initiation  Start codon signals where the gene begins (at 5’ end of mRNA)  Ribosome binding site on the mRNA binds to a small ribosomal subunit  Then this complex binds to a large ribosomal subunit forming the complete ...
no sigma falls off after initiation
no sigma falls off after initiation

... Polycistronic mRNAs are formed by post-transcriptional splicing of small transcripts. would be interesting, but no! More interesting if bits of protein could! ...
Jumbo_2860g_strawberry_2014 copy - Supplements
Jumbo_2860g_strawberry_2014 copy - Supplements

... in the proven method of keeping a food log and the best practical solution left for most may be to rely on the daily use of a JUMBO type shake that can really help to take in enough nutrients to progress. With 50 grams of one of the highest quality protein per serving, a large dose of energy dense 6 ...
Proteins, Enzymes, Nucleic Acids Proteins What are the buildi
Proteins, Enzymes, Nucleic Acids Proteins What are the buildi

... Lactose is a molecule broken down by the enzyme lactase. People who are lactose intolerant do not have significant amounts of lactase, so lactose is broken down by bacterial colonies that causes gastrointestinal distress. One way of treating this is to use lactase on milk before bottling it. This wi ...
Recent Advances in Directed Protein Evolution
Recent Advances in Directed Protein Evolution

... phages display pIII on the surface bacteriophage pIII is required pIII ...
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Two-hybrid screening



Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.
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