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L6 Proteins of cereals and legumes - e
L6 Proteins of cereals and legumes - e

... phytic acid phosphate esters bind to the cationic group of basic amino acids, for example, arginine, histidine and lysine, may form insoluble phytateprotein complexes. At a pH above the isoelectric point of proteins, the charge of proteins as well as that of the phytic acid is negative – direct inte ...
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... charged with formylmethionine tRNAfMet • These components bind with an mRNA at the ribosome-binding site, RBS or the Shine–Dalgarno sequence. Together, they recruit a 50S sub-unit • mRNA molecules contain information for the amino acid sequences of several different proteins; such a molecule is call ...
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... e. disulfide bridges― covalent bonds between two cysteine amino acids with sulfhydryl groups are brought close enough together by the folding of the protein D. Quaternary Structure― when two or more polypeptide chains are joined together by bonds or interactions of their R–groups. Same as a–e above. ...
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Two-hybrid screening



Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.
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