and phylogenetic characterization of Shuni virus Genomic
... 25–27] (Fig. S1), is less well conserved within the Simbu serogroup. Based on alignment with these viruses and comparison to the data obtained by Yanase et al. [9], protease cleavage between Gn/NSm of SAE18/09 occurs after the conserved arginine (R) 308 residue (Fig. S1). The cleavage site between N ...
... 25–27] (Fig. S1), is less well conserved within the Simbu serogroup. Based on alignment with these viruses and comparison to the data obtained by Yanase et al. [9], protease cleavage between Gn/NSm of SAE18/09 occurs after the conserved arginine (R) 308 residue (Fig. S1). The cleavage site between N ...
10 white P20 2 - Metabolomics Workbench
... bottom right of box). Of the three black switches, set the start/reset switch to neutral. Set the heat switch to neutral. Set the gas switch to Manual. To obtain gas flow, turn the gas nozzle on right side of hood. Turn the Harris valve in hood to open position. Adjust LPM air to no more than 15. Pl ...
... bottom right of box). Of the three black switches, set the start/reset switch to neutral. Set the heat switch to neutral. Set the gas switch to Manual. To obtain gas flow, turn the gas nozzle on right side of hood. Turn the Harris valve in hood to open position. Adjust LPM air to no more than 15. Pl ...
Regulation of Galactoside Transport by the PTS
... isolated and extensively characterized in enteric bacteria such as E. coli and Salmonella typhimurium. Some of mutations were mapped in genes for enzyme I and HPr, common components of the PTS. Although it had been suggested that the PTS is responsible for the uptake of many carbohydrates, it has be ...
... isolated and extensively characterized in enteric bacteria such as E. coli and Salmonella typhimurium. Some of mutations were mapped in genes for enzyme I and HPr, common components of the PTS. Although it had been suggested that the PTS is responsible for the uptake of many carbohydrates, it has be ...
Urea cycle
... liver, dissolved in blood (in a concentration of 2.5 - 7.5 mM), and secreted by the kidney. Urea also plays a very important role in protein catabolism, removal of toxic ammonia from the body. Urea determination is very useful for the medical clinician to assess kidney and other organs function ...
... liver, dissolved in blood (in a concentration of 2.5 - 7.5 mM), and secreted by the kidney. Urea also plays a very important role in protein catabolism, removal of toxic ammonia from the body. Urea determination is very useful for the medical clinician to assess kidney and other organs function ...
P4-0065 RNA/DNA/Protein Purification Kit
... Notes Prior to Use • Blood of all human and animal subjects is considered potentially infectious. All necessary precautions recommended by the appropriate authorities in the country of use should be taken when working with whole blood. • It is recommended that no more than 100 µL of blood be used in ...
... Notes Prior to Use • Blood of all human and animal subjects is considered potentially infectious. All necessary precautions recommended by the appropriate authorities in the country of use should be taken when working with whole blood. • It is recommended that no more than 100 µL of blood be used in ...
as Powerpoint presentation
... This process is similar in mitochondria and in many bacteria. Phosphorylation of ADP to ATP is catalysed by a membrane-bound ATP synthase (also called ATPase). The energy for this is provided by the oxidation of NADH2 by oxygen, catalysed by an electron transport chain. This sequence of redox reacti ...
... This process is similar in mitochondria and in many bacteria. Phosphorylation of ADP to ATP is catalysed by a membrane-bound ATP synthase (also called ATPase). The energy for this is provided by the oxidation of NADH2 by oxygen, catalysed by an electron transport chain. This sequence of redox reacti ...
Differential mRNA expression levels and gene sequences of a
... clone from the resistant strain. This nucleotide change results in an amino acid difference in the predicted protein sequence from tryptophan (Trp220) in the susceptible strain to a glycine (Gly220) in the resistant strain. The point mutation at position 658 was confirmed in the genomic DNA sequence ...
... clone from the resistant strain. This nucleotide change results in an amino acid difference in the predicted protein sequence from tryptophan (Trp220) in the susceptible strain to a glycine (Gly220) in the resistant strain. The point mutation at position 658 was confirmed in the genomic DNA sequence ...
Structure and expression of the PHO80 gene of Saccharomyces
... requires the trans-acting factor PHO80. We have determined the DNA sequence of the PHO80 gene and found that it encodes a protein of 293 amino acids. The expression of the PHO80 gene, as measured by Northern analysis and level of a PHO80-LacZ fusion protein is independent of the level of phosphate i ...
... requires the trans-acting factor PHO80. We have determined the DNA sequence of the PHO80 gene and found that it encodes a protein of 293 amino acids. The expression of the PHO80 gene, as measured by Northern analysis and level of a PHO80-LacZ fusion protein is independent of the level of phosphate i ...
Biochem10 - Amit Kessel Ph.D
... A. acetylation B. methylation C. phosphorylation D. dephosphorylation E. deamination 52. Molecular cloning of eukaryotic mRNA sequences utilizes all of the following EXCEPT A. DNA polymerase. B. reverse transcriptase. C. restriction endonucleases. D. RNA polymerase. E. oligo dT primers. 53. A cloned ...
... A. acetylation B. methylation C. phosphorylation D. dephosphorylation E. deamination 52. Molecular cloning of eukaryotic mRNA sequences utilizes all of the following EXCEPT A. DNA polymerase. B. reverse transcriptase. C. restriction endonucleases. D. RNA polymerase. E. oligo dT primers. 53. A cloned ...
Expressing the carotenoid biosynthesis pathway in
... Create a transgenic strain of Saccharomyces cerevisiae that produces β-carotene, the precursor to vitamin A When this GMO yeast is added to bread or other baked goods, it produces β-carotene in addition to its normal byproducts ...
... Create a transgenic strain of Saccharomyces cerevisiae that produces β-carotene, the precursor to vitamin A When this GMO yeast is added to bread or other baked goods, it produces β-carotene in addition to its normal byproducts ...
Identification of a novel N-terminal hydrophobic sequence that
... their location by immunofluorescence. Substituting alanine for each of the first five sets of amino acids (Fig. 1, AS1-5) had no effect on targeting to droplets (for example, AS2, supplementary material Fig. S1D-F). By contrast, substituting alanine for regions 6 or 7 markedly reduced targeting to d ...
... their location by immunofluorescence. Substituting alanine for each of the first five sets of amino acids (Fig. 1, AS1-5) had no effect on targeting to droplets (for example, AS2, supplementary material Fig. S1D-F). By contrast, substituting alanine for regions 6 or 7 markedly reduced targeting to d ...
paper describing HSSP
... which one performs searches or from which one derives structural preferences. For example, in order to have on the average 5 occurrences of all possible 8000 amino acid triplets in each of 3 secondary structure states (helix, extended strand, loop) one would need a database of about 120000 residues ...
... which one performs searches or from which one derives structural preferences. For example, in order to have on the average 5 occurrences of all possible 8000 amino acid triplets in each of 3 secondary structure states (helix, extended strand, loop) one would need a database of about 120000 residues ...
1 - GET Test Bank
... 28. Cysteine often plays an important role in stabilizing protein structure. Explain how this works. Ans: Two adjacent sulfhydryl (SH) groups can oxidize to form a covalent disulfide (S-S) bond. Disulfide bonds can stabilize the structure of folded peptides or sometimes hold two separate peptide cha ...
... 28. Cysteine often plays an important role in stabilizing protein structure. Explain how this works. Ans: Two adjacent sulfhydryl (SH) groups can oxidize to form a covalent disulfide (S-S) bond. Disulfide bonds can stabilize the structure of folded peptides or sometimes hold two separate peptide cha ...
Comparison of the Structure of the Extrinsic 33 kDa Protein from
... by which the DNA fragments of 5¢- and 3¢-flanking regions of the 33 kDa protein cDNA were amplified using primers newly synthesized based on the 469-bp cDNA fragment, which yielded a 565-bp cDNA fragment and a 640-bp cDNA fragment from 5¢- and 3¢-RACE, respectively. The nucleotide sequences of these ...
... by which the DNA fragments of 5¢- and 3¢-flanking regions of the 33 kDa protein cDNA were amplified using primers newly synthesized based on the 469-bp cDNA fragment, which yielded a 565-bp cDNA fragment and a 640-bp cDNA fragment from 5¢- and 3¢-RACE, respectively. The nucleotide sequences of these ...
Comparative proteomic network signatures in seminal plasma of
... dependent on the overall abundance of the proteins. It has been reported [37] that accurate quantification and determination of real biological change is dependent on the number of SpCs and hence, different constraints have to be applied to SpC levels in order to circumvent the biases and maintain a ...
... dependent on the overall abundance of the proteins. It has been reported [37] that accurate quantification and determination of real biological change is dependent on the number of SpCs and hence, different constraints have to be applied to SpC levels in order to circumvent the biases and maintain a ...
Ideas on Cell Respiration Demos
... A prop I found very useful was a wooden dowel, a plastic disk, fishing line, and extra-fuzzy pipe cleaners. Out of this I fashioned a 3-dimensional spindle to demonstrate mitosis and meiosis phases. Unfortunately, I could not do cytokinesis with this model. This year I challenged my AP students to f ...
... A prop I found very useful was a wooden dowel, a plastic disk, fishing line, and extra-fuzzy pipe cleaners. Out of this I fashioned a 3-dimensional spindle to demonstrate mitosis and meiosis phases. Unfortunately, I could not do cytokinesis with this model. This year I challenged my AP students to f ...
MORE ABOUT SOLUTIONS - Bio-Link
... Once gloves have come in contact with a surface that was touched by skin (for example, a pen, notebook, laboratory bench, etc.) the gloves should be changed ...
... Once gloves have come in contact with a surface that was touched by skin (for example, a pen, notebook, laboratory bench, etc.) the gloves should be changed ...
alignment-2005
... • The count matrix is used to estimate a mutation matrix at 1 PAM (evolutionary unit) • From the mutation matrix, a Dayhoff scoring matrix is constructed • This Dayhoff matrix along with a model of indel events is then used to score new alignments which can then be used in an iterative process to ...
... • The count matrix is used to estimate a mutation matrix at 1 PAM (evolutionary unit) • From the mutation matrix, a Dayhoff scoring matrix is constructed • This Dayhoff matrix along with a model of indel events is then used to score new alignments which can then be used in an iterative process to ...
Structural Insights into Triglyceride Storage Mediated by
... FIT1 and FIT2 are six-transmembrane-domain-containing proteins FIT1 and FIT2 comprise a unique family of proteins that do not have homology to known proteins or protein domains found in any species. In order to create a foundation for structure-function analysis of FIT proteins, we sought to create ...
... FIT1 and FIT2 are six-transmembrane-domain-containing proteins FIT1 and FIT2 comprise a unique family of proteins that do not have homology to known proteins or protein domains found in any species. In order to create a foundation for structure-function analysis of FIT proteins, we sought to create ...
EF-TU - Soran University
... EF-Tu-GTP binds & delivers an aminoacyl-tRNA to the A site on the ribosome. EF-Tu recognizes & binds all aminoacyl-tRNAs with approx. the same affinity, when each tRNA is bonded to the correct (cognate) amino acid. tRNAs for different amino acids have evolved to differ slightly EF-Tu colored red in ...
... EF-Tu-GTP binds & delivers an aminoacyl-tRNA to the A site on the ribosome. EF-Tu recognizes & binds all aminoacyl-tRNAs with approx. the same affinity, when each tRNA is bonded to the correct (cognate) amino acid. tRNAs for different amino acids have evolved to differ slightly EF-Tu colored red in ...
article in press - Biochemistry
... through metabolic pathways. These signal transduction pathways appear to operate both independently from and coordinately with the hormonal pathways. Since several of these pathways are conserved from the unicellular yeasts to mammals, they must have originally evolved independent of hormonal contro ...
... through metabolic pathways. These signal transduction pathways appear to operate both independently from and coordinately with the hormonal pathways. Since several of these pathways are conserved from the unicellular yeasts to mammals, they must have originally evolved independent of hormonal contro ...
Chemical synthesis of proteins
... an amide bond and regenerating the free sulfhydryl on the Cys. The ligation strategy has been extended further to make use of other thiol additives and their respective reactivities78. The trityl-associated mercaptoproprionic-acid leucine (TAMPAL) resin allows the convenient generation of any amino ...
... an amide bond and regenerating the free sulfhydryl on the Cys. The ligation strategy has been extended further to make use of other thiol additives and their respective reactivities78. The trityl-associated mercaptoproprionic-acid leucine (TAMPAL) resin allows the convenient generation of any amino ...
Table S10. List of the Databases and Software used in the H
... This database mainly uses sequence homology analyses and features extensive utilization of information on three-dimensional structures. ...
... This database mainly uses sequence homology analyses and features extensive utilization of information on three-dimensional structures. ...
Two-hybrid screening
Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.