Chloroplast phosphoglycerate kinase from Euglena gracilis
... cpPGK from isolated Euglena chloroplasts also yielded two peaks of PGK activity eluting at nearly the same salt concentrations from Reactive Blue 72 and Mono Q (data not shown). These findings strongly suggest that two very similar isoforms of the chloroplast PGK were purified from total Euglena cells ...
... cpPGK from isolated Euglena chloroplasts also yielded two peaks of PGK activity eluting at nearly the same salt concentrations from Reactive Blue 72 and Mono Q (data not shown). These findings strongly suggest that two very similar isoforms of the chloroplast PGK were purified from total Euglena cells ...
Sequence-based predictions of membrane-protein topology, homology and insertion
... molecules across barriers, and converting light into sugar, among many other things. The specific function of a protein molecule is determined from its three-dimensional shape, which in turn is a function only of its amino acid sequence (Anfinsen 1973), and ultimately of its corresponding DNA sequen ...
... molecules across barriers, and converting light into sugar, among many other things. The specific function of a protein molecule is determined from its three-dimensional shape, which in turn is a function only of its amino acid sequence (Anfinsen 1973), and ultimately of its corresponding DNA sequen ...
PDF
... that some partial decapitates die. However, there are striking reductions in the electrophoretic activity, and thus presumably amounts, of alkaline phosphatase and a cathodal esterase. The reduction in alkaline phosphatase is of interest in that Hinni & Watterson (1963) found this enzyme was reduced ...
... that some partial decapitates die. However, there are striking reductions in the electrophoretic activity, and thus presumably amounts, of alkaline phosphatase and a cathodal esterase. The reduction in alkaline phosphatase is of interest in that Hinni & Watterson (1963) found this enzyme was reduced ...
+ O 2
... gp91phox and p22phox. Activation of the complex also involves guanine nucleotide exchange on the GTP-binding protein RAC stimulated by guanine nucleotide exchange factors. Guanine nucleotide exchange on RAC is associated with release of RhoGDI and translocation of RAC from the cytosol to the NOX com ...
... gp91phox and p22phox. Activation of the complex also involves guanine nucleotide exchange on the GTP-binding protein RAC stimulated by guanine nucleotide exchange factors. Guanine nucleotide exchange on RAC is associated with release of RhoGDI and translocation of RAC from the cytosol to the NOX com ...
A bioinformatics study concerning the structural and functional
... caveolar membranes directly interacting with cav1 to form a stable hetero-oligomeric complex that is required for targeting to lipid rafts and for caveolae formation.20 It has three isoforms: α, β and γ. The α- and β-isoforms differ in their subcellular locations and the β-isoform does not interact ...
... caveolar membranes directly interacting with cav1 to form a stable hetero-oligomeric complex that is required for targeting to lipid rafts and for caveolae formation.20 It has three isoforms: α, β and γ. The α- and β-isoforms differ in their subcellular locations and the β-isoform does not interact ...
Detection of Free Radicals
... gp91phox and p22phox. Activation of the complex also involves guanine nucleotide exchange on the GTP-binding protein RAC stimulated by guanine nucleotide exchange factors. Guanine nucleotide exchange on RAC is associated with release of RhoGDI and translocation of RAC from the cytosol to the NOX com ...
... gp91phox and p22phox. Activation of the complex also involves guanine nucleotide exchange on the GTP-binding protein RAC stimulated by guanine nucleotide exchange factors. Guanine nucleotide exchange on RAC is associated with release of RhoGDI and translocation of RAC from the cytosol to the NOX com ...
Simulation of Enzyme Reaction - diss.fu
... The electrostatic models used today consider these effects, however sometimes only implicitly. In standard empirical force fields used for Molecular Dynamics or Monte Carlo simulations all atoms of a molecular system are represented in detail. All electrostatic interactions between the corresponding ...
... The electrostatic models used today consider these effects, however sometimes only implicitly. In standard empirical force fields used for Molecular Dynamics or Monte Carlo simulations all atoms of a molecular system are represented in detail. All electrostatic interactions between the corresponding ...
Metabolic and physiological interdependencies in the
... together constituted 2.11 OrgNSAF% in the thiotrophic B. azoricus symbiont (for comparison: the Riftia symbiont’s rhodanese proteins make up only 0.15 OrgNSAF %, n = 3; S. Markert, unpublished). The B. azoricus thiotroph clearly oxidizes sulfide, as indicated by the detection of most components of t ...
... together constituted 2.11 OrgNSAF% in the thiotrophic B. azoricus symbiont (for comparison: the Riftia symbiont’s rhodanese proteins make up only 0.15 OrgNSAF %, n = 3; S. Markert, unpublished). The B. azoricus thiotroph clearly oxidizes sulfide, as indicated by the detection of most components of t ...
Sequence, expression, and characterization of the first archaeal ATP
... DNA ligase and coenzymes were from Roche Diagnostics (Mannheim, Germany), New England Biolabs (Beverly, Mass., USA), and PEQLAB (Erlangen, Germany). Substrates were from Roche Diagnostics (ATP, ADP, F-6-P), Merck (fructose), and Sigma (F-1-P, ribose, ribose 5-phosphate, adenosine). Gases were from L ...
... DNA ligase and coenzymes were from Roche Diagnostics (Mannheim, Germany), New England Biolabs (Beverly, Mass., USA), and PEQLAB (Erlangen, Germany). Substrates were from Roche Diagnostics (ATP, ADP, F-6-P), Merck (fructose), and Sigma (F-1-P, ribose, ribose 5-phosphate, adenosine). Gases were from L ...
Intersubunit contacts are often facilitated by specificity
... The LacI family of bacterial transcription factors ...
... The LacI family of bacterial transcription factors ...
Chapter 10
... (codon). Although it is a redundant code, it is not an ambiguous code: under normal circumstances, a given codon encodes one and only one amino acid. In addition to the 20 amino acids, there are also three “stop codons” dedicated to ending translation. The three stop codons also have colloquial name ...
... (codon). Although it is a redundant code, it is not an ambiguous code: under normal circumstances, a given codon encodes one and only one amino acid. In addition to the 20 amino acids, there are also three “stop codons” dedicated to ending translation. The three stop codons also have colloquial name ...
Phylogenetic analysis
... In order to determine the validity of your tree you’ll need to bootstrap it. Since our sequence isn’t very long, only a couple hundred replications are needed. Now click the check button, then click ‘Compute’ in the main window… ...
... In order to determine the validity of your tree you’ll need to bootstrap it. Since our sequence isn’t very long, only a couple hundred replications are needed. Now click the check button, then click ‘Compute’ in the main window… ...
THE INFLUENCE OF BODY MASS INDEX (BMI)
... no longer produce the required amino acid or not produce sufficient amounts to meet demand, the amino acid becomes conditionally essential. Peptide bonds bind amino acids to one another and can be thought of as the glue that holds them together (1). The peptide bond connects the acid end of one amin ...
... no longer produce the required amino acid or not produce sufficient amounts to meet demand, the amino acid becomes conditionally essential. Peptide bonds bind amino acids to one another and can be thought of as the glue that holds them together (1). The peptide bond connects the acid end of one amin ...
FASTA is a program for database searching by homology. FASTA
... characters long and these words are organized into a table indicating where they are in the sequence. If one or more words are present in both sequences, and especially if several words can be joined, the sequences must be similar in those regions. This method is used from the web site http://fasta. ...
... characters long and these words are organized into a table indicating where they are in the sequence. If one or more words are present in both sequences, and especially if several words can be joined, the sequences must be similar in those regions. This method is used from the web site http://fasta. ...
Recombinant Mouse Pentraxin 3/TSG-14
... Similar to CRP and SAP, TSG14 binds to the complement cascade component C1q (6). However, TSG14 does not bind to phosphoethanolamine, phosphocholine, or high pyruvate agarose, which are known ligands for CRP and SAP. While CRP and SAP are primarily produced in the liver, TSG14 expression is stro ...
... Similar to CRP and SAP, TSG14 binds to the complement cascade component C1q (6). However, TSG14 does not bind to phosphoethanolamine, phosphocholine, or high pyruvate agarose, which are known ligands for CRP and SAP. While CRP and SAP are primarily produced in the liver, TSG14 expression is stro ...
The Biologically Appropriate Food Concept
... functions, including cellular regeneration, tissue maintenance, hormone and enzyme production, and the provision of energy. Although protein is essential, not all proteins function equally, with protein qualities varying enormously between various sources. Three factors effecting protein quality inc ...
... functions, including cellular regeneration, tissue maintenance, hormone and enzyme production, and the provision of energy. Although protein is essential, not all proteins function equally, with protein qualities varying enormously between various sources. Three factors effecting protein quality inc ...
Affinity Chromatography
... In this seminal paper, the general principles and potential application of affinity chromatography were enunciated and have largely remained unchanged until the present date. The paper contained several important contributions. First, it generalized the technique to all potential enzyme purification ...
... In this seminal paper, the general principles and potential application of affinity chromatography were enunciated and have largely remained unchanged until the present date. The paper contained several important contributions. First, it generalized the technique to all potential enzyme purification ...
Module 5 - MIMG — UCLA
... the telephone about what they did that day. Bob is only interested in three activities: walking in the park, shopping, and cleaning his apartment. The choice of what to do is determined exclusively by the weather on a given day. Alice has no definite information about the weather where Bob lives, bu ...
... the telephone about what they did that day. Bob is only interested in three activities: walking in the park, shopping, and cleaning his apartment. The choice of what to do is determined exclusively by the weather on a given day. Alice has no definite information about the weather where Bob lives, bu ...
genetic code and tra..
... The 4 nucleotide bases (A,G,C and U) in mRNA are used to produce the three base codons. There are therefore, 64 codons code for the 20 amino acids, and since each codon code for only one amino acids this means that, there are more than one cone for the same amino acid. How to translate a codon (see ...
... The 4 nucleotide bases (A,G,C and U) in mRNA are used to produce the three base codons. There are therefore, 64 codons code for the 20 amino acids, and since each codon code for only one amino acids this means that, there are more than one cone for the same amino acid. How to translate a codon (see ...
Identification of a molecular component of the mitochondrial
... However, eukaryotic orthologues to Pat have not been identified in either the mitochondrial or nuclear genome [9]. An alternative scenario in eukaryotes could be that mitochondrial proteins are acetylated in the cytosol prior to mitochondrial import. However, as fasting and feeding result in a dynam ...
... However, eukaryotic orthologues to Pat have not been identified in either the mitochondrial or nuclear genome [9]. An alternative scenario in eukaryotes could be that mitochondrial proteins are acetylated in the cytosol prior to mitochondrial import. However, as fasting and feeding result in a dynam ...
Patent Protection for the Protein Products of Recombinant DNA
... resulting from the identification, isolation, and expression of a native cellular gene- it is not surprising that the biotechnology industry has ag15 gressively sought patent protection for its efforts. To be patented, a product must satisfy the three statutory requirements of utility, novelty, and ...
... resulting from the identification, isolation, and expression of a native cellular gene- it is not surprising that the biotechnology industry has ag15 gressively sought patent protection for its efforts. To be patented, a product must satisfy the three statutory requirements of utility, novelty, and ...
random
... • Distant relationships are often best detected by motif or profile searches rather than pair-wise comparisons • PSI-BLAST first performs a BLAST search. • PSI-BLAST uses the information from significant BLAST alignments returned to construct a position specific score matrix, which replaces the quer ...
... • Distant relationships are often best detected by motif or profile searches rather than pair-wise comparisons • PSI-BLAST first performs a BLAST search. • PSI-BLAST uses the information from significant BLAST alignments returned to construct a position specific score matrix, which replaces the quer ...
Two-hybrid screening
Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.