A Method To Define the Carboxyl Terminal of Proteins
... precursor m/z value for the entire LC-MS/MS run. The m/z values used in these MS/MS analyses were determined by MALDI-MS characterization, initially assuming that the charge state of the peptide ions of interest observed in the electrospray ionization measurement was 2+. For the MS/MS analyses shown ...
... precursor m/z value for the entire LC-MS/MS run. The m/z values used in these MS/MS analyses were determined by MALDI-MS characterization, initially assuming that the charge state of the peptide ions of interest observed in the electrospray ionization measurement was 2+. For the MS/MS analyses shown ...
Additional file 1
... Receptor like protein kinase (ACM89561.1) Leucine-rich repeat (LRR) family protein (AT3G05990.1) ...
... Receptor like protein kinase (ACM89561.1) Leucine-rich repeat (LRR) family protein (AT3G05990.1) ...
Chem 100 Unit 5 Biochemistry
... hydrophilic head. One of the major functions of Sphingolipids and phosphoglycerides is forming the “lipid bilayer” of cell membranes. Glycolipids are found in brain and nervous tissue. ...
... hydrophilic head. One of the major functions of Sphingolipids and phosphoglycerides is forming the “lipid bilayer” of cell membranes. Glycolipids are found in brain and nervous tissue. ...
Chapter 8
... Mutants that don’t produce i gene product express the operon even when lactose is not available. This implies that the normal i gene product is a repressor, which blocks transcription when bound to o. When lactose is present in normal cells, it binds to the repressor, preventing it from binding to t ...
... Mutants that don’t produce i gene product express the operon even when lactose is not available. This implies that the normal i gene product is a repressor, which blocks transcription when bound to o. When lactose is present in normal cells, it binds to the repressor, preventing it from binding to t ...
CHAPTER FOUR U n
... Representational Difference Analysis (RDA) was first described by Lisitsyn et al. (1993) for the identification of difference between two complex genomes. RDA belongs to the general class of DNA subtractive methodologies, in which one DNA population, known as the “driver”, is hybridized in excess ag ...
... Representational Difference Analysis (RDA) was first described by Lisitsyn et al. (1993) for the identification of difference between two complex genomes. RDA belongs to the general class of DNA subtractive methodologies, in which one DNA population, known as the “driver”, is hybridized in excess ag ...
Sequences of the Nucleocapsid Genes from Two Strains of Avian
... complete double-stranded sequencing of both clones. For M41 the region covered is only 1652 bases since neither M41 clone contains a sequence of 184 bases that is present in the Beaudette clones. In Fig. 2 the top line of sequence is IBV Beaudette, with the bases that are different in M41 marked und ...
... complete double-stranded sequencing of both clones. For M41 the region covered is only 1652 bases since neither M41 clone contains a sequence of 184 bases that is present in the Beaudette clones. In Fig. 2 the top line of sequence is IBV Beaudette, with the bases that are different in M41 marked und ...
How an Organism`s Genotype Determines Its Phenotype How an
... everything after the mutation is shifted and would likely code for a new sequence of AAs © 2013 Pearson Education, Inc. ...
... everything after the mutation is shifted and would likely code for a new sequence of AAs © 2013 Pearson Education, Inc. ...
Colicins produced by the Escherichia fergusonii strains closely
... The colicin E1 cea gene encoded by this plasmid had a one-nucleotide replacement (G211A) when compared to cea of pColE1-EF43, resulting in a one-amino acid change (A71T) in colicin E1 protein (Fig. 1). pColE1-EF3 imm and kil gene sequences were identical to those of pColE1-EF43. Consistent with this ...
... The colicin E1 cea gene encoded by this plasmid had a one-nucleotide replacement (G211A) when compared to cea of pColE1-EF43, resulting in a one-amino acid change (A71T) in colicin E1 protein (Fig. 1). pColE1-EF3 imm and kil gene sequences were identical to those of pColE1-EF43. Consistent with this ...
Rapid Publication - Journal of Clinical Investigation
... The lymphoid cell line GM-1 715 and the fibroblast line GM-2445 were derived from the same patient with ADA deficiency (6). It has been suggested previously that this patient's phenotype may have resulted from underproduction of a normal enzyme rather than from any abnormality of protein structure, ...
... The lymphoid cell line GM-1 715 and the fibroblast line GM-2445 were derived from the same patient with ADA deficiency (6). It has been suggested previously that this patient's phenotype may have resulted from underproduction of a normal enzyme rather than from any abnormality of protein structure, ...
Globins in Nonvertebrate Species: Dispersal by Horizontal Gene
... The NV globin template (table 2) was constructed as follows. One hundred forty five NV globin amino acid sequences were aligned using the following guidelines: (1) Related globins with known secondary structure were used as references for matching the helical segments. When the appropriate crystal s ...
... The NV globin template (table 2) was constructed as follows. One hundred forty five NV globin amino acid sequences were aligned using the following guidelines: (1) Related globins with known secondary structure were used as references for matching the helical segments. When the appropriate crystal s ...
PowerPoint
... structure rather than sequence and used the resulting sequence alignments as their database from which to gather substitution statistics. In principle, the Rissler or Overington matrices should give more reliable results than either PAM or BLOSUM. However, the comparatively small number of available ...
... structure rather than sequence and used the resulting sequence alignments as their database from which to gather substitution statistics. In principle, the Rissler or Overington matrices should give more reliable results than either PAM or BLOSUM. However, the comparatively small number of available ...
Proteomic analyses of Oryza sativa mature pollen reveal novel
... poorly understood. Here, we identified 322 unique proteins, most of which were not reported previously to be in pollen, from mature pollen of Oryza sativa L. ssp japonica using a proteomic approach, 23% of them having more than one isoform. Functional classification reveals that an overrepresentatio ...
... poorly understood. Here, we identified 322 unique proteins, most of which were not reported previously to be in pollen, from mature pollen of Oryza sativa L. ssp japonica using a proteomic approach, 23% of them having more than one isoform. Functional classification reveals that an overrepresentatio ...
GT-B fold
... Only two general folds, termed GT-A and GT-B (and variants), have been observed for all structures of nucleotide-sugar-dependent GTs solved to date ...
... Only two general folds, termed GT-A and GT-B (and variants), have been observed for all structures of nucleotide-sugar-dependent GTs solved to date ...
Evolution of Function in Protein Superfamilies, from a Structural
... The recent growth in protein databases has revealed the functional diversity of many protein superfamilies. We have assessed the functional variation of homologous enzyme superfamilies containing two or more enzymes, as de®ned by the CATH protein structure classi®cation, by way of the Enzyme Commiss ...
... The recent growth in protein databases has revealed the functional diversity of many protein superfamilies. We have assessed the functional variation of homologous enzyme superfamilies containing two or more enzymes, as de®ned by the CATH protein structure classi®cation, by way of the Enzyme Commiss ...
Supplementary Notes - Word file
... Supplementary Figure 8. Model of acute transcriptional repression mediated by ING2 recognition of trimethylated H3K4. Active genes are marked by trimethylated H3K4 via the activity of H3K4 histone methyltransferases. In response to a cellular stress, such as DNA damage, pro-proliferative and pro-sur ...
... Supplementary Figure 8. Model of acute transcriptional repression mediated by ING2 recognition of trimethylated H3K4. Active genes are marked by trimethylated H3K4 via the activity of H3K4 histone methyltransferases. In response to a cellular stress, such as DNA damage, pro-proliferative and pro-sur ...
The variable and conserved interfaces of modeled olfactory receptor
... of the OR model. We observed that out of 36 hypervariable residues ~whose variability value is more than one standard deviation above the average variability in the TM segments!, in the entire OR protein, 26 are in the three variable helices, most of which ~20 residues! are on the predicted inner su ...
... of the OR model. We observed that out of 36 hypervariable residues ~whose variability value is more than one standard deviation above the average variability in the TM segments!, in the entire OR protein, 26 are in the three variable helices, most of which ~20 residues! are on the predicted inner su ...
Mapping Enzyme Active Sites in Complex Proteomes
... hydrolases5,7 and cysteine proteases.8 In each of these cases, the designed probes have been shown to label their target enzymes in an activity-based manner, distinguishing, for example, active enzymes from their inactive zymogens and/or inhibitor-bound forms.5,7,8 Although directed ABPP has proven ...
... hydrolases5,7 and cysteine proteases.8 In each of these cases, the designed probes have been shown to label their target enzymes in an activity-based manner, distinguishing, for example, active enzymes from their inactive zymogens and/or inhibitor-bound forms.5,7,8 Although directed ABPP has proven ...
Carbohydrates
... Carbohydrates are classified as either "simple" or "complex". Some common simple carbohydrates include glucose, fructose, and sucrose, and are typically associated with sweet foods and ripe fruits. The complex carbohydrates are either digestible (starches) or indigestible (fiber). The digestible com ...
... Carbohydrates are classified as either "simple" or "complex". Some common simple carbohydrates include glucose, fructose, and sucrose, and are typically associated with sweet foods and ripe fruits. The complex carbohydrates are either digestible (starches) or indigestible (fiber). The digestible com ...
Nuclear mitotic apparatus protein (NuMA): spindle association
... their sequences were identical, except for six sequence blocks (75 bp, 42 bp, 45 bp, 576 bp, 1012 bp and 212 bp), which were either inserted or deleted in individual cDNA clones. Among these, two sequence blocks (75 bp and 42 bp), which encode a 25 and a 14 amino acid peptide, respectively, are loca ...
... their sequences were identical, except for six sequence blocks (75 bp, 42 bp, 45 bp, 576 bp, 1012 bp and 212 bp), which were either inserted or deleted in individual cDNA clones. Among these, two sequence blocks (75 bp and 42 bp), which encode a 25 and a 14 amino acid peptide, respectively, are loca ...
and phylogenetic characterization of Shuni virus Genomic
... 25–27] (Fig. S1), is less well conserved within the Simbu serogroup. Based on alignment with these viruses and comparison to the data obtained by Yanase et al. [9], protease cleavage between Gn/NSm of SAE18/09 occurs after the conserved arginine (R) 308 residue (Fig. S1). The cleavage site between N ...
... 25–27] (Fig. S1), is less well conserved within the Simbu serogroup. Based on alignment with these viruses and comparison to the data obtained by Yanase et al. [9], protease cleavage between Gn/NSm of SAE18/09 occurs after the conserved arginine (R) 308 residue (Fig. S1). The cleavage site between N ...
Two-hybrid screening
Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.