E-mail: - HAL
... Many research groups have designed fragment libraries or structural alphabets to try to describe the local structural features of known protein structures more accurately.17-29 These libraries or alphabets correspond to finite sets of protein structural fragments. They can be differentiated accordin ...
... Many research groups have designed fragment libraries or structural alphabets to try to describe the local structural features of known protein structures more accurately.17-29 These libraries or alphabets correspond to finite sets of protein structural fragments. They can be differentiated accordin ...
A Chemical Look at Proteins: Workhorses of the Cell
... Many of the molecules found in the cell are polymers, which are large molecules comprised of repeating monomer units. We began by talking about the structure and function of nucleic acid polymers of DNA and RNA. These polymers are comprised of only four different building blocks each and they are hi ...
... Many of the molecules found in the cell are polymers, which are large molecules comprised of repeating monomer units. We began by talking about the structure and function of nucleic acid polymers of DNA and RNA. These polymers are comprised of only four different building blocks each and they are hi ...
PDF
... Biophysics, CAS, Beijing 100101, China; 4Genome Center, University of Washington, Seattle, Washington 98195, USA Thermoanaerobacter tengcongensis is a rod-shaped, gram-negative, anaerobic eubacterium that was isolated from a freshwater hot spring in Tengchong, China. Using a whole-genome-shotgun met ...
... Biophysics, CAS, Beijing 100101, China; 4Genome Center, University of Washington, Seattle, Washington 98195, USA Thermoanaerobacter tengcongensis is a rod-shaped, gram-negative, anaerobic eubacterium that was isolated from a freshwater hot spring in Tengchong, China. Using a whole-genome-shotgun met ...
Protein and DNA sequence determinants of
... adaptation. While the nucleotide composition biases appear to be largely due to adaptation at the level of amino acid compositions, an additional DNA sequence adaptation that is independent of amino acid composition adaptation is still possible – at the level of higher order correlation in nucleotid ...
... adaptation. While the nucleotide composition biases appear to be largely due to adaptation at the level of amino acid compositions, an additional DNA sequence adaptation that is independent of amino acid composition adaptation is still possible – at the level of higher order correlation in nucleotid ...
Function and biotechnology of extremophilic enzymes in low water
... diminishing the number of intermolecular hydrogen bonds [20-22]. High salt concentrations critically affect the solubility, binding, stability, and crystallization of proteins [23]. The interactions between proteins and protein subunits in solution are also altered by salts. The electrostatic intera ...
... diminishing the number of intermolecular hydrogen bonds [20-22]. High salt concentrations critically affect the solubility, binding, stability, and crystallization of proteins [23]. The interactions between proteins and protein subunits in solution are also altered by salts. The electrostatic intera ...
Eris - Molecules in Action, LLC
... In flexible-‐backbone stability prediction, Eris performs similar calculations as that in the fixed-‐backbone approach, except that it ...
... In flexible-‐backbone stability prediction, Eris performs similar calculations as that in the fixed-‐backbone approach, except that it ...
3. G-protein-coupled receptors
... 3.7. Glycogen metabolism - triggered by adrenaline in liver cells (2 slides) 3.8. GI proteins 3.9. Phosphorylation ...
... 3.7. Glycogen metabolism - triggered by adrenaline in liver cells (2 slides) 3.8. GI proteins 3.9. Phosphorylation ...
3. G-protein-coupled receptors
... 3.7. Glycogen metabolism - triggered by adrenaline in liver cells (2 slides) 3.8. GI proteins 3.9. Phosphorylation ...
... 3.7. Glycogen metabolism - triggered by adrenaline in liver cells (2 slides) 3.8. GI proteins 3.9. Phosphorylation ...
Improving penicillin biosynthesis in Penicillium chrysogenum
... Genetic engineering of fungal cell factories mainly focuses on manipulating enzymes of the product pathway or primary metabolism. However, despite the use of strong promoters or strains containing the genes of interest in multiple copies, the desired strongly enhanced enzyme levels are often not obt ...
... Genetic engineering of fungal cell factories mainly focuses on manipulating enzymes of the product pathway or primary metabolism. However, despite the use of strong promoters or strains containing the genes of interest in multiple copies, the desired strongly enhanced enzyme levels are often not obt ...
45_Biochemistry of Muscles
... skeletal muscle related to its metabolism • Skeletal muscle contains phosphocreatine, which acts as an energy store for short-term (seconds) demands. • Free fatty acids in plasma are a major source of energy, particularly under marathon conditions and in prolonged starvation. • Skeletal muscle can u ...
... skeletal muscle related to its metabolism • Skeletal muscle contains phosphocreatine, which acts as an energy store for short-term (seconds) demands. • Free fatty acids in plasma are a major source of energy, particularly under marathon conditions and in prolonged starvation. • Skeletal muscle can u ...
Two fatty acid ∆9-desaturase genes, ole1 and ole2
... ∆9-1 and ∆9-2, each containing a single intron, were cloned from strain CBS 528.72 while one cDNA clone, LM9, was isolated from strain CBS 210.32. The ∆9-1 gene encoded a protein of 445 aa which shared 99 % identity with the LM9 gene product. These proteins also showed 40–60 % identity to the ∆9desa ...
... ∆9-1 and ∆9-2, each containing a single intron, were cloned from strain CBS 528.72 while one cDNA clone, LM9, was isolated from strain CBS 210.32. The ∆9-1 gene encoded a protein of 445 aa which shared 99 % identity with the LM9 gene product. These proteins also showed 40–60 % identity to the ∆9desa ...
Pore-Exposed Tyrosine Residues of P
... including helices 5 and 11 (Parveen et al., 2011) (Fig. 1). These results imply the existence of two substrate-transporter interaction modes. Full transporters have arisen from half transporters by gene duplication. In homodimeric half transporters, each conformation of any set of amino acid residue ...
... including helices 5 and 11 (Parveen et al., 2011) (Fig. 1). These results imply the existence of two substrate-transporter interaction modes. Full transporters have arisen from half transporters by gene duplication. In homodimeric half transporters, each conformation of any set of amino acid residue ...
Reactivities of HIV-1 gag-Derived Peptides with Antibodies of HIV
... background values due to unspecific binding to artificial structures. In general, reactivities of individual sera, when tested on gag-derived peptides, were relatively low showing values for optical density between 0.5 and 1.5. Higher reaction values were seen only occasionally and preferentially wi ...
... background values due to unspecific binding to artificial structures. In general, reactivities of individual sera, when tested on gag-derived peptides, were relatively low showing values for optical density between 0.5 and 1.5. Higher reaction values were seen only occasionally and preferentially wi ...
tRNA aminoacylation by arginyltRNA synthetase: induced
... expected canonical structure of a free tRNA, as found in yeast tRNAAsp or yeast tRNAPhe for example. The conformation of this loop is characterized by three structural features: (i) the formation of a bulge at the level of Ade38; (ii) the intercalation of Ade37 between the last base pair of the anti ...
... expected canonical structure of a free tRNA, as found in yeast tRNAAsp or yeast tRNAPhe for example. The conformation of this loop is characterized by three structural features: (i) the formation of a bulge at the level of Ade38; (ii) the intercalation of Ade37 between the last base pair of the anti ...
Integrative Assignment - California State University
... on page 47 of your book, tally the hydrophobic ones, and divide by the total number of amino acids in the protein. Should take about 10 minutes. There is also a website that does the calculations for you (see next slide), but it is frequently more trouble than it is worth. http://mobyle.pasteur.fr/c ...
... on page 47 of your book, tally the hydrophobic ones, and divide by the total number of amino acids in the protein. Should take about 10 minutes. There is also a website that does the calculations for you (see next slide), but it is frequently more trouble than it is worth. http://mobyle.pasteur.fr/c ...
APC2 Antibody
... promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take ...
... promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take ...
Document
... Proteins perform biological functions such as structural support, catalysis of chemical reactions, immune response to foreign substances, transport of molecules across membranes, and control of genetic expression. The three-dimensional structure and function of a protein is determined by the sequenc ...
... Proteins perform biological functions such as structural support, catalysis of chemical reactions, immune response to foreign substances, transport of molecules across membranes, and control of genetic expression. The three-dimensional structure and function of a protein is determined by the sequenc ...
Evolution of an ancient protein function involved in
... that is localized on the cell’s cortex by external signals (Figure 1A) (Lu and Johnston, 2013; Johnston et al., 2009; Siegrist and Doe, 2005; Siegrist, 2006). The complex and its functions have been most extensively studied in Drosophila melanogaster neuroblasts, but it plays a similar role in birds ...
... that is localized on the cell’s cortex by external signals (Figure 1A) (Lu and Johnston, 2013; Johnston et al., 2009; Siegrist and Doe, 2005; Siegrist, 2006). The complex and its functions have been most extensively studied in Drosophila melanogaster neuroblasts, but it plays a similar role in birds ...
LecturesPart07
... Basic Method: For two sequences of lengths M and N, lay out an M by N grid (matrix) with one sequence across the top and one sequence down the left side. For each position in the grid, compare the sequence elements at the top (column) and to the left (row). If and only if they are the same, place a ...
... Basic Method: For two sequences of lengths M and N, lay out an M by N grid (matrix) with one sequence across the top and one sequence down the left side. For each position in the grid, compare the sequence elements at the top (column) and to the left (row). If and only if they are the same, place a ...
Two-hybrid screening
Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.