Coenzyme B 12-Dependent Ribonucleotide Reductase: Evidence
... cofactor. Recent cloning, sequencing, and expression of this protein [Booker, S., & Stubbe, J. (1993) Proc. Natl. Acad. Sci. U.S.A.90,8352-83561 have now allowed its characterization by site-directed mutagenesis. The present study focuses on the role of five cysteines postulated to be required for c ...
... cofactor. Recent cloning, sequencing, and expression of this protein [Booker, S., & Stubbe, J. (1993) Proc. Natl. Acad. Sci. U.S.A.90,8352-83561 have now allowed its characterization by site-directed mutagenesis. The present study focuses on the role of five cysteines postulated to be required for c ...
Origin and evolution of peptide-modifying
... (2OGFeDO) with the double-stranded b-helix fold (DSBH), which includes numerous enzymes acting on diverse substrates such as: amino acids and proteins (e.g. EGL-9, prolyl and lysyl hydroxylases), small molecules (e.g. clavaminate synthase, isopenicillin synthase and plant leukoanthocyanin hydroxylas ...
... (2OGFeDO) with the double-stranded b-helix fold (DSBH), which includes numerous enzymes acting on diverse substrates such as: amino acids and proteins (e.g. EGL-9, prolyl and lysyl hydroxylases), small molecules (e.g. clavaminate synthase, isopenicillin synthase and plant leukoanthocyanin hydroxylas ...
Turf M.D.: The Attributes of Amino Acids
... Given that single amino acids can be taken up by the plant readily and that partially hydrolyzed proteins (dipeptide or larger) are not taken up until microbial activity occurs, the analogies of "quick-release" and "slow-release" types of amino acids have been applied. On some fertilizer bags that c ...
... Given that single amino acids can be taken up by the plant readily and that partially hydrolyzed proteins (dipeptide or larger) are not taken up until microbial activity occurs, the analogies of "quick-release" and "slow-release" types of amino acids have been applied. On some fertilizer bags that c ...
The Family of SMF Metal Ion Transporters in Yeast Cells*
... The antibody detection system (ECL) was from Amersham Pharmacia Biotech. Western blots were performed according to the protocol of the ECL antibody detection system from the manufacturer. Samples were denatured by SDS sample buffer and electrophoresed on 12% polyacrylamide Mini gels (Bio-Rad) as des ...
... The antibody detection system (ECL) was from Amersham Pharmacia Biotech. Western blots were performed according to the protocol of the ECL antibody detection system from the manufacturer. Samples were denatured by SDS sample buffer and electrophoresed on 12% polyacrylamide Mini gels (Bio-Rad) as des ...
Brooker Chapter 14
... Inhibitors bind to activators and prevent them from binding to DNA Genes that are regulated in this manner are termed repressible Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display ...
... Inhibitors bind to activators and prevent them from binding to DNA Genes that are regulated in this manner are termed repressible Copyright ©The McGraw-Hill Companies, Inc. Permission required for reproduction or display ...
Mader 11 ch 3 Chemistry of Organic Molecules Part 2
... disulfide bond Quaternary Structure This level of structure occurs when two or more folded polypeptides interact to perform a biological function. ...
... disulfide bond Quaternary Structure This level of structure occurs when two or more folded polypeptides interact to perform a biological function. ...
3.2 Breeding of corn line DAS-40278-9
... amino acid sequence similarity to known protein toxins or allergens and digestibility studies have demonstrated that the protein would be rapidly degraded following ingestion, similar to other dietary proteins. Taken together, the evidence indicates that the AAD-1 protein is neither toxic nor likely ...
... amino acid sequence similarity to known protein toxins or allergens and digestibility studies have demonstrated that the protein would be rapidly degraded following ingestion, similar to other dietary proteins. Taken together, the evidence indicates that the AAD-1 protein is neither toxic nor likely ...
Probing the active site of homoserine trans
... 3.1. Homoserine trans-succinylase yields four spots on 2D gels In a strain of E. coli K12 (MG1655, pUCmetA) overexpressing the metA gene, four proteins appeared on a 2D gel, which could not be detected in the wild-type strain, not carrying the metA plasmid. These spots corresponded to HTS, as could ...
... 3.1. Homoserine trans-succinylase yields four spots on 2D gels In a strain of E. coli K12 (MG1655, pUCmetA) overexpressing the metA gene, four proteins appeared on a 2D gel, which could not be detected in the wild-type strain, not carrying the metA plasmid. These spots corresponded to HTS, as could ...
Caenorhabditis elegans unc-60 gene encodes
... structure and function. Many of these genes have known homologs in vertebrate muscle, unc-54, for example, encodes one of the four myosin isoforms, uric-22 (Benian et al. 1989) is similar to the proteins titin and projectin, and unc-52 encodes a protein that resembles perlecan (Rogalski et al. 1993) ...
... structure and function. Many of these genes have known homologs in vertebrate muscle, unc-54, for example, encodes one of the four myosin isoforms, uric-22 (Benian et al. 1989) is similar to the proteins titin and projectin, and unc-52 encodes a protein that resembles perlecan (Rogalski et al. 1993) ...
The demonstration of nickel in the urease of Helicobacter pylori by
... saline. Briefly, 100 /zl of the fraction (starting at 1 : 10 dilution) was incubated at room temperature with 100 /~1 of 100 mM urea containing 0.2% (w/v) phenol red. After 10 rain the absorbance was read at 540 nm. ...
... saline. Briefly, 100 /zl of the fraction (starting at 1 : 10 dilution) was incubated at room temperature with 100 /~1 of 100 mM urea containing 0.2% (w/v) phenol red. After 10 rain the absorbance was read at 540 nm. ...
Enzymes
... Enzyme : A biological catalyst. Usually a globular protein molecule produced by living organisms that can speed up a specific chemical reaction without itself being destroyed or changed in any way. K m: (Michaelis constant) The substrate concentration at which an enzyme catalysed reaction procee ...
... Enzyme : A biological catalyst. Usually a globular protein molecule produced by living organisms that can speed up a specific chemical reaction without itself being destroyed or changed in any way. K m: (Michaelis constant) The substrate concentration at which an enzyme catalysed reaction procee ...
Restriction Digest of pAMP and pKAN
... You might have predicted that when loaded into the electrophoresis gel, K- and A- would produce only a single DNA band; there’s no reason why you would think otherwise. However, it is likely that two or three bands will appear in the undigested K- and A- plasmid lanes. This is because plasmids isol ...
... You might have predicted that when loaded into the electrophoresis gel, K- and A- would produce only a single DNA band; there’s no reason why you would think otherwise. However, it is likely that two or three bands will appear in the undigested K- and A- plasmid lanes. This is because plasmids isol ...
Chapter 26
... • Well-nourished adult meets 80% to 90% of resting energy needs from fat – Fat is superior to carbohydrates for energy storage for two reasons • Carbohydrates are hydrophilic, absorb water, and expand and occupy more space in the tissues, and fat is hydrophobic, contains almost no water, and is a mo ...
... • Well-nourished adult meets 80% to 90% of resting energy needs from fat – Fat is superior to carbohydrates for energy storage for two reasons • Carbohydrates are hydrophilic, absorb water, and expand and occupy more space in the tissues, and fat is hydrophobic, contains almost no water, and is a mo ...
biochemistry-n-6-protein-metabolism
... an endopeptidase, cleaving peptide bonds at various points within the protein chain. Smaller peptides and some free amino acids are produced. ...
... an endopeptidase, cleaving peptide bonds at various points within the protein chain. Smaller peptides and some free amino acids are produced. ...
FES, Active FES, Active
... Step 5. Initiate the reaction by the addition of 5 µl [33P]-ATP Assay Cocktail bringing the final volume up to 25µl and incubate the mixture in a water bath at 30oC for 15 minutes. Step 6. After the 15 minute incubation period, terminate the reaction by spotting 20 µl of the reaction mixture onto in ...
... Step 5. Initiate the reaction by the addition of 5 µl [33P]-ATP Assay Cocktail bringing the final volume up to 25µl and incubate the mixture in a water bath at 30oC for 15 minutes. Step 6. After the 15 minute incubation period, terminate the reaction by spotting 20 µl of the reaction mixture onto in ...
Chapter 3 The Molecules of Cells
... 3.2 A few chemical groups are key to the functioning of biological molecules The functional groups are – hydroxyl group—consists of a hydrogen bonded to an oxygen, – carbonyl group—a carbon linked by a double bond to an oxygen atom, – carboxyl group—consists of a carbon double-bonded to both a ...
... 3.2 A few chemical groups are key to the functioning of biological molecules The functional groups are – hydroxyl group—consists of a hydrogen bonded to an oxygen, – carbonyl group—a carbon linked by a double bond to an oxygen atom, – carboxyl group—consists of a carbon double-bonded to both a ...
... Alternatively, if you stated no, your answer was graded according to the following scheme: +1 pt: for stating the peaks for lysozyme and phospholipase would overlap. +1.5 pts: for stating a workable purification scheme (e.g. isoelectric focusing, affinity chromatography, ion exchange chromatography. ...
Supplementary Figures 1 - 5, Methods
... protein content. Aliquots (2mg/ml of protein) of samples were incubated at 4°C ...
... protein content. Aliquots (2mg/ml of protein) of samples were incubated at 4°C ...
the lecture in Powerpoint Format
... 3.13 A protein’s shape depends on four levels of structure The overall three-dimensional shape of a polypeptide is called its tertiary structure. – Tertiary structure generally results from interactions between the R groups of the various amino acids. – Disulfide bridges may further strengthen th ...
... 3.13 A protein’s shape depends on four levels of structure The overall three-dimensional shape of a polypeptide is called its tertiary structure. – Tertiary structure generally results from interactions between the R groups of the various amino acids. – Disulfide bridges may further strengthen th ...
Toxin in bullous impetigo and staphylococcal scalded
... 1 h at room temperature. After two washes with 0.1% Tween-20 in TBS, the sheets were incubated for 2 h with horseradish peroxidase-conjugated antibody against rabbit or rat immunoglobulin G (BioRad Laboratories), diluted 1:1,000 in blocking buffer. After the blots were washed, the signals were detec ...
... 1 h at room temperature. After two washes with 0.1% Tween-20 in TBS, the sheets were incubated for 2 h with horseradish peroxidase-conjugated antibody against rabbit or rat immunoglobulin G (BioRad Laboratories), diluted 1:1,000 in blocking buffer. After the blots were washed, the signals were detec ...
NSPKU
... finger foods. Over time, they will get more of their Phe exchanges from food (e.g. potato or permitted cereal). Less exchanges will come from breast milk or formula milk. Phe free foods should still be included in the diet as well. Your child will also continue their gradual switch from their infant ...
... finger foods. Over time, they will get more of their Phe exchanges from food (e.g. potato or permitted cereal). Less exchanges will come from breast milk or formula milk. Phe free foods should still be included in the diet as well. Your child will also continue their gradual switch from their infant ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.