Localization of protein-binding sites within families of proteins
... We address the question of whether or not the positions of protein-binding sites on homologous protein structures are conserved irrespective of the identities of their binding partners. First, for each domain family in the Structural Classification of Proteins (SCOP), protein-binding sites are extra ...
... We address the question of whether or not the positions of protein-binding sites on homologous protein structures are conserved irrespective of the identities of their binding partners. First, for each domain family in the Structural Classification of Proteins (SCOP), protein-binding sites are extra ...
Environmentally Induced Changes in Amino Acid Composition in the
... threonine; Tyr: tyrosine; Val: valine; S.E.: standard error of means. b Values followed by the same letter in a column are not significantly different according to Duncan’s test (p < 0.05). ...
... threonine; Tyr: tyrosine; Val: valine; S.E.: standard error of means. b Values followed by the same letter in a column are not significantly different according to Duncan’s test (p < 0.05). ...
Eris - Molecules in Action, LLC
... distance (10 Angstrom) are allowed to move during MC steps. The distance is measured as the smallest distance between any pair of atoms between the two residues. ...
... distance (10 Angstrom) are allowed to move during MC steps. The distance is measured as the smallest distance between any pair of atoms between the two residues. ...
Essential Bioinformatics and Biocomputing
... • If we blindly interpret these results, we would erroneously conclude that motif VLADIMIR may have some functional importance for structure or function of the strawberry vein binding virus, and that TINWEE has to do with calciumdependent protein kinase in rice and possibly in Legionella pneumophila ...
... • If we blindly interpret these results, we would erroneously conclude that motif VLADIMIR may have some functional importance for structure or function of the strawberry vein binding virus, and that TINWEE has to do with calciumdependent protein kinase in rice and possibly in Legionella pneumophila ...
3 | biological macromolecules
... Benefits of Carbohydrates Are carbohydrates good for you? People who wish to lose weight are often told that carbohydrates are bad for them and should be avoided. Some diets completely forbid carbohydrate consumption, claiming that a low-carbohydrate diet helps people to lose weight faster. However, ...
... Benefits of Carbohydrates Are carbohydrates good for you? People who wish to lose weight are often told that carbohydrates are bad for them and should be avoided. Some diets completely forbid carbohydrate consumption, claiming that a low-carbohydrate diet helps people to lose weight faster. However, ...
From Sequence to Structure
... Hydrophobic amino-acid residues engage in van der Waals interactions only. Their tendency to avoid contact with water and pack against each other is the basis for the hydrophobic effect. Alanine and leucine are strong helix-favoring residues, while proline is rarely found in helices because its back ...
... Hydrophobic amino-acid residues engage in van der Waals interactions only. Their tendency to avoid contact with water and pack against each other is the basis for the hydrophobic effect. Alanine and leucine are strong helix-favoring residues, while proline is rarely found in helices because its back ...
The size, operation, and technical capabilities of protein and nucleic
... range of investigators, not only specialists in these areas but also cellular and molecular biologists in general (1). An investigator with little or no training in protein sequencing can now use a relatively simple technique, sodium dodecyl sulfate (SDS) gel electrophoresis followed by electroblott ...
... range of investigators, not only specialists in these areas but also cellular and molecular biologists in general (1). An investigator with little or no training in protein sequencing can now use a relatively simple technique, sodium dodecyl sulfate (SDS) gel electrophoresis followed by electroblott ...
Information Sheet - HJ Baker & Bro., Inc.
... A proper balance of amino acids is required to synthesize proteins. Animal performance is limited by the most deficient amino acid. Supplemental amino acids such as lysine and methionine are often used to meet requirements of the target species. However, unlike fish, shrimp do not consume feeds quic ...
... A proper balance of amino acids is required to synthesize proteins. Animal performance is limited by the most deficient amino acid. Supplemental amino acids such as lysine and methionine are often used to meet requirements of the target species. However, unlike fish, shrimp do not consume feeds quic ...
2013_BJ_SCFBio_Website
... http://www.riethoven.org/BioInformer/newsletter/archives/2/procrustes.html ...
... http://www.riethoven.org/BioInformer/newsletter/archives/2/procrustes.html ...
Isolation of a Strong Suppressor of Nonsense Mutations in Bacillus
... B. subtilis MO-101-P su- grown in minimal medium until a cell concentration of 1Q8/ml,was concentrated 2-fold by centrifugation and resuspension in the same medium. An aliquot was diluted 4-fold in 1 M Tris-HC1 pH 7.5 in the presence of 4% (v/v) ethyl methanesulfonate [17]. The mixture was shaken st ...
... B. subtilis MO-101-P su- grown in minimal medium until a cell concentration of 1Q8/ml,was concentrated 2-fold by centrifugation and resuspension in the same medium. An aliquot was diluted 4-fold in 1 M Tris-HC1 pH 7.5 in the presence of 4% (v/v) ethyl methanesulfonate [17]. The mixture was shaken st ...
Isolation of a UV Endonuclease from the
... the UV endonucleases of E. coli and Micrococcus luteus have been shown to be stimulated by, but not dependent upon Mg2+. Assays were routinely carried out for 15 rnin at 37 OC under yellow, non-photoreactivating light (550-700 nm). Reactions were started by adding the lysate or enzyme containing fra ...
... the UV endonucleases of E. coli and Micrococcus luteus have been shown to be stimulated by, but not dependent upon Mg2+. Assays were routinely carried out for 15 rnin at 37 OC under yellow, non-photoreactivating light (550-700 nm). Reactions were started by adding the lysate or enzyme containing fra ...
1 - Universitas Brawijaya
... Proteins that catalyze (speed up) chemical reactions without being used up or destroyed in the process. Anabolic (putting things together) and catabolic (breaking things down) functions. ...
... Proteins that catalyze (speed up) chemical reactions without being used up or destroyed in the process. Anabolic (putting things together) and catabolic (breaking things down) functions. ...
Expression profiles of Ciona tailbud embryos - Development
... multiple tissues. The study also highlighted characteristic gene expression profiles dependent on the tissues. In addition, several genes showed intriguing expression patterns that have not been reported previously; for example, four genes were expressed specifically in the nerve cord cells and one ...
... multiple tissues. The study also highlighted characteristic gene expression profiles dependent on the tissues. In addition, several genes showed intriguing expression patterns that have not been reported previously; for example, four genes were expressed specifically in the nerve cord cells and one ...
Enzyme
... allows you to both see enzyme kinetics happening and secondly how it is affected by different factors ...
... allows you to both see enzyme kinetics happening and secondly how it is affected by different factors ...
Lehninger Principles of Biochemistry 5/e
... AMP-activated protein kinase - regulated by [AMP] - A reduced nutrient supply or by increase exercise cause the rise in [AMP] - increase glucose uptake, activates glycolysis and fatty acid oxidation - suppress energy requiring processes such as fatty acid, cholesterol, and ...
... AMP-activated protein kinase - regulated by [AMP] - A reduced nutrient supply or by increase exercise cause the rise in [AMP] - increase glucose uptake, activates glycolysis and fatty acid oxidation - suppress energy requiring processes such as fatty acid, cholesterol, and ...
Novel surface layer protein genes in Bacillus
... separated on a 1 % agarose gel (SeakemLE). Southern blots were performed on positively charged nylon membranes (Roche) using a TurboBlotter (Schleicher & Schuell), following the manufacturer’s instructions. Northern blotting of total RNA was performed according to published methods (Sambrook et al., ...
... separated on a 1 % agarose gel (SeakemLE). Southern blots were performed on positively charged nylon membranes (Roche) using a TurboBlotter (Schleicher & Schuell), following the manufacturer’s instructions. Northern blotting of total RNA was performed according to published methods (Sambrook et al., ...
Amino Acids, Proteins, and Enzymes
... for the second half of the lab • When your group has agreed on the procedure share it with me for approval • Once it is approved everyone needs to write the procedure into their lab handout • All written procedures, observations, and questions need to be completed in your own words ...
... for the second half of the lab • When your group has agreed on the procedure share it with me for approval • Once it is approved everyone needs to write the procedure into their lab handout • All written procedures, observations, and questions need to be completed in your own words ...
Chromatography
... • Larger molecules may penetrate pores above a certain size so they spend less time in the bed. • The larger molecules elute first, while the smaller molecules travel slower [because they move into and out of more of the pores] and elute later. ...
... • Larger molecules may penetrate pores above a certain size so they spend less time in the bed. • The larger molecules elute first, while the smaller molecules travel slower [because they move into and out of more of the pores] and elute later. ...
Molecular and Structural Characterization of
... and 60 kD (TaGlu1b) that correspond to the two bands of the natural glucosidases purified from wheat shoots (Sue et al., 2000b). The same difference in mobility was evident with the N-His-tagged glucosidases (Fig. 3A). The precise Mr of N-His-tagged TaGlu1a and TaGlu1b was determined by mass spectro ...
... and 60 kD (TaGlu1b) that correspond to the two bands of the natural glucosidases purified from wheat shoots (Sue et al., 2000b). The same difference in mobility was evident with the N-His-tagged glucosidases (Fig. 3A). The precise Mr of N-His-tagged TaGlu1a and TaGlu1b was determined by mass spectro ...
Fatty acid
... • Collagen is a fibrous protein consisting of three polypeptides coiled like a rope • Hemoglobin is a globular protein consisting of four polypeptides: two alpha and two beta chains Animation: Quaternary Protein Structure Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummi ...
... • Collagen is a fibrous protein consisting of three polypeptides coiled like a rope • Hemoglobin is a globular protein consisting of four polypeptides: two alpha and two beta chains Animation: Quaternary Protein Structure Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummi ...
PLANT PROTEIN PHOSPHATASES
... residues (107). Phosphohistidine phosphorylation has also been reported in plants (50), fungi (86), and animals (25), but its relative contribution to the total phosphoamino acid content of eukaryotic cells is not known. In animals, protein phosphorylation plays well-known roles in diverse cellular ...
... residues (107). Phosphohistidine phosphorylation has also been reported in plants (50), fungi (86), and animals (25), but its relative contribution to the total phosphoamino acid content of eukaryotic cells is not known. In animals, protein phosphorylation plays well-known roles in diverse cellular ...
03-232 Biochemistry Exam III - S2014 Name:________________________
... oil is a triglyceride that melts at 25oC and is therefore a solid at room temperature. What is the most likely difference between the fatty acids in these two oils and how does this difference affect their melting temperatures? What fundamental thermodynamic interaction (e.g. H-bonds, electrostatics ...
... oil is a triglyceride that melts at 25oC and is therefore a solid at room temperature. What is the most likely difference between the fatty acids in these two oils and how does this difference affect their melting temperatures? What fundamental thermodynamic interaction (e.g. H-bonds, electrostatics ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.