biochemistry - Louis Bolk Institute
... Studying this module will make biochemistry more interesting and easier to remember as well as increase our respect for nature. The questions posed within the boxes do not have definitive answers yet. They are meant to stimulate further research. ...
... Studying this module will make biochemistry more interesting and easier to remember as well as increase our respect for nature. The questions posed within the boxes do not have definitive answers yet. They are meant to stimulate further research. ...
PLANT PROTEIN PHOSPHATASES
... residues (107). Phosphohistidine phosphorylation has also been reported in plants (50), fungi (86), and animals (25), but its relative contribution to the total phosphoamino acid content of eukaryotic cells is not known. In animals, protein phosphorylation plays well-known roles in diverse cellular ...
... residues (107). Phosphohistidine phosphorylation has also been reported in plants (50), fungi (86), and animals (25), but its relative contribution to the total phosphoamino acid content of eukaryotic cells is not known. In animals, protein phosphorylation plays well-known roles in diverse cellular ...
LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
... What is the role of nicotinamide adenine dinucleotide in an enzyme reaction? Why is it important to maintain a specific pH for each enzyme reaction? Explain P.C. Stemmer’s first experiment with in vitro recombination. What is the application of alkaline phosphatase in genetic engineering? What is th ...
... What is the role of nicotinamide adenine dinucleotide in an enzyme reaction? Why is it important to maintain a specific pH for each enzyme reaction? Explain P.C. Stemmer’s first experiment with in vitro recombination. What is the application of alkaline phosphatase in genetic engineering? What is th ...
Partial purification of fatty acid synthetase from Streptomyces
... mol.wt. 180000. A similar purification was obtained after sucrose-gradient centrifugation to give material with s20,w 8 S. Thus the synthetase migrated as a single species during these Filamentous bacteria of the genus Streptomyces are extremely procedures (with a consistent recovery of over 80%), a ...
... mol.wt. 180000. A similar purification was obtained after sucrose-gradient centrifugation to give material with s20,w 8 S. Thus the synthetase migrated as a single species during these Filamentous bacteria of the genus Streptomyces are extremely procedures (with a consistent recovery of over 80%), a ...
Dynamics of Plasmodium falciparum enoyl‐ACP reductase and
... protein, residues 325 through 366 are generally not resolved.1,21 Although partly unresolved, these residues are critical because residues in the N- and C-terminal ten-residue fragments of the loop are known to interact with drugs in the substrate-binding site. Additionally, a conserved salt bridge ...
... protein, residues 325 through 366 are generally not resolved.1,21 Although partly unresolved, these residues are critical because residues in the N- and C-terminal ten-residue fragments of the loop are known to interact with drugs in the substrate-binding site. Additionally, a conserved salt bridge ...
Dairy 17 - Milk Urea Nitrogen
... • Breakdown of excess amino acids in the liver. Excessive production of urea is associated with inefficient use of protein in dairy cow diets and has been implicated with reductions in dairy herd fertility in some situations. Most of the urea produced is removed via urine but some passes into the mi ...
... • Breakdown of excess amino acids in the liver. Excessive production of urea is associated with inefficient use of protein in dairy cow diets and has been implicated with reductions in dairy herd fertility in some situations. Most of the urea produced is removed via urine but some passes into the mi ...
Research Communications
... functional chloroplasts are known to be encoded by nuclear DNA. These proteins are synthesized in their precursor forms with an amino-terminal signal peptide called the transit peptide 1. A signature peptide sequence (chloroplast target peptide) drives protein into chloroplast and gets cleaved off a ...
... functional chloroplasts are known to be encoded by nuclear DNA. These proteins are synthesized in their precursor forms with an amino-terminal signal peptide called the transit peptide 1. A signature peptide sequence (chloroplast target peptide) drives protein into chloroplast and gets cleaved off a ...
Sensory control of longevity by the ODR-3 and GPA
... only one of these G proteins, odr-3, seems directly involved in the detection of environmental cues and might couple directly with odorant or gustatory receptors11,12,14,17. All G proteins, including odr-3, play a role in behaviors that involve changes of the sensitivity of the sensory neurons: modu ...
... only one of these G proteins, odr-3, seems directly involved in the detection of environmental cues and might couple directly with odorant or gustatory receptors11,12,14,17. All G proteins, including odr-3, play a role in behaviors that involve changes of the sensitivity of the sensory neurons: modu ...
Molecular characteristics of sucrose synthase
... SuSy isoformes of analysed enzyme which were distinguished in presented studies have also been found in several eudicotyledons, i.e. potato, carrot, Japanese pear fruit and BY-2 tobacco heterotrophic cells [5, 6, 8]. Matic et al. [6] suggested that SuSyI is more involved in channeling metabolites in ...
... SuSy isoformes of analysed enzyme which were distinguished in presented studies have also been found in several eudicotyledons, i.e. potato, carrot, Japanese pear fruit and BY-2 tobacco heterotrophic cells [5, 6, 8]. Matic et al. [6] suggested that SuSyI is more involved in channeling metabolites in ...
A Chemical Look at Proteins: Workhorses of the Cell
... acids a single amino acid change would have no effect. Often this is the case, but sometimes a single change makes a profound difference. This slide shows one example where a single amino acid change alters the activity of a protein significantly. Hemoglobin, the oxygen carrier in red blood cells, c ...
... acids a single amino acid change would have no effect. Often this is the case, but sometimes a single change makes a profound difference. This slide shows one example where a single amino acid change alters the activity of a protein significantly. Hemoglobin, the oxygen carrier in red blood cells, c ...
NMEICT PROJECT
... study the enzyme kinetics. It has many applications in enzyme kinetics. It helps in understanding the rates of reactions which assist in judging the kind of reaction that enzyme follows. (single- or multiple-substrate mechanism). Enzyme activity measures the amount of enzyme in a reaction. There are ...
... study the enzyme kinetics. It has many applications in enzyme kinetics. It helps in understanding the rates of reactions which assist in judging the kind of reaction that enzyme follows. (single- or multiple-substrate mechanism). Enzyme activity measures the amount of enzyme in a reaction. There are ...
Utilisation of Whey
... Immunoglobulins. The immunoglobulins in whey are antibodies, of the types IgA, IgG, IgE and IgM, with molecular weights of 150,000 to 450,000 or greater. Some of them represent the normal antibody components of the cow and are the same as those found in the bloodstream; however, there is a secretory ...
... Immunoglobulins. The immunoglobulins in whey are antibodies, of the types IgA, IgG, IgE and IgM, with molecular weights of 150,000 to 450,000 or greater. Some of them represent the normal antibody components of the cow and are the same as those found in the bloodstream; however, there is a secretory ...
SUPPLEMENTARY INFORMATION
... Comparison with CNT Although the amino acid sequence varies, the structure of the hairpin tip – capping loop motif is conserved between the two proteins, suggesting that this structural motif may be a common Na+binding motif in membrane transporter proteins. While vcINDY and CNT share a similar core ...
... Comparison with CNT Although the amino acid sequence varies, the structure of the hairpin tip – capping loop motif is conserved between the two proteins, suggesting that this structural motif may be a common Na+binding motif in membrane transporter proteins. While vcINDY and CNT share a similar core ...
on the enzyme
... On the enzyme, there is a place where the target molecule can attach. This place is called the active site. The target molecule/chemical is the substrate. ...
... On the enzyme, there is a place where the target molecule can attach. This place is called the active site. The target molecule/chemical is the substrate. ...
The Low-Down on Low-Carbohydrate Diets
... ingested kilojoules are burned in the process. The conversion of dietary fat to body fat, on the other hand, uses only 3% of the ingested kilojoules. In other words, more kilojoules are burned and fewer are stored when excess kilojoules come from carbohydrate than when they come from fat. Carbohydra ...
... ingested kilojoules are burned in the process. The conversion of dietary fat to body fat, on the other hand, uses only 3% of the ingested kilojoules. In other words, more kilojoules are burned and fewer are stored when excess kilojoules come from carbohydrate than when they come from fat. Carbohydra ...
Identification, Expression and Characterization of Archaeal
... 6.1.3. P. pastoris Culture............................................................................................. 78 6.1.3.1. Preparation of P. pastoris competent cells ................................................. 79 6.1.3.2. Transformation and expression Chop-1SS in P. pastoris (GS115).. ...
... 6.1.3. P. pastoris Culture............................................................................................. 78 6.1.3.1. Preparation of P. pastoris competent cells ................................................. 79 6.1.3.2. Transformation and expression Chop-1SS in P. pastoris (GS115).. ...
Amino acid residues that determine functional specificity of NADP
... high. One can describe four-group SDPs roughly as a union of cofactor-specific and substrate-specific SDPs, together with some new positions. This can be explained by the fact that dividing the family into four groups account for both types of specificity, whereas the other two divisions ignore one of ...
... high. One can describe four-group SDPs roughly as a union of cofactor-specific and substrate-specific SDPs, together with some new positions. This can be explained by the fact that dividing the family into four groups account for both types of specificity, whereas the other two divisions ignore one of ...
Descriptions of translation related genes that
... L-homoserine-O-acetyltransferase, catalyzes the conversion of homoserine to O-acetyl homoserine which is the first step of the methionine biosynthetic pathway 3-phosphoserine aminotransferase, catalyzes the formation of phosphoserine from 3-phosphohydroxypyruvate, required for serine and glycine bio ...
... L-homoserine-O-acetyltransferase, catalyzes the conversion of homoserine to O-acetyl homoserine which is the first step of the methionine biosynthetic pathway 3-phosphoserine aminotransferase, catalyzes the formation of phosphoserine from 3-phosphohydroxypyruvate, required for serine and glycine bio ...
Early and late endosomal compartments of Entamoeba histolytica
... the associated proteins which may regulate endocytic or secretory function. In other eukaryotes, the Rab family of small molecular weight Ras-like GTPases play important roles in the regulation of vesicle trafficking between various compartments along endo-lysosomal and secretory pathways [15, 16]. ...
... the associated proteins which may regulate endocytic or secretory function. In other eukaryotes, the Rab family of small molecular weight Ras-like GTPases play important roles in the regulation of vesicle trafficking between various compartments along endo-lysosomal and secretory pathways [15, 16]. ...
Secondary structure prediction method (SOPMA)
... Argus Lab is a molecular modeling program that runs on Windows 98, NT, and 2000. Argus Lab consists of a user interface that supports OpenGL graphics display of molecule structures and runs quantum mechanical calculations using the Argus compute server. The Argus compute server is constructed using ...
... Argus Lab is a molecular modeling program that runs on Windows 98, NT, and 2000. Argus Lab consists of a user interface that supports OpenGL graphics display of molecule structures and runs quantum mechanical calculations using the Argus compute server. The Argus compute server is constructed using ...
Safety Assessment of Soy Proteins and Peptides as Used in
... Definition and Manufacture The definitions and functions of the soy peptide ingredients included in this report are provided in Table 1. The soy protein and peptide derivatives form a broad category of materials which are prepared by extraction from soy and partial hydrolysis to yield cosmetic ingre ...
... Definition and Manufacture The definitions and functions of the soy peptide ingredients included in this report are provided in Table 1. The soy protein and peptide derivatives form a broad category of materials which are prepared by extraction from soy and partial hydrolysis to yield cosmetic ingre ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.