UACA / Nucling (Nuclear Membrane Marker) Antibody
... autoantigen UACA in patients with panuveitis. Biochem. Biophys. Res. Commun. 280: 1169-1176. | Ohkura, T., et al. 2004. Detection of the novel autoantibody (anti-UACA antibody) in patients with Graves disease. Biochem. Biophys. Res. Commun. 321: 432-440 ...
... autoantigen UACA in patients with panuveitis. Biochem. Biophys. Res. Commun. 280: 1169-1176. | Ohkura, T., et al. 2004. Detection of the novel autoantibody (anti-UACA antibody) in patients with Graves disease. Biochem. Biophys. Res. Commun. 321: 432-440 ...
Amino acid sequence fingerprints in divergent evolution of
... e-mail: [email protected] Proteins may basically be related by divergent or convergent evolution. In divergent evolution, i.e. the evolution from a common ancestor, there is only a small percentage of residues in the amino acid sequence of a protein, that have to be conserved in order the prot ...
... e-mail: [email protected] Proteins may basically be related by divergent or convergent evolution. In divergent evolution, i.e. the evolution from a common ancestor, there is only a small percentage of residues in the amino acid sequence of a protein, that have to be conserved in order the prot ...
Cartoon modeling of proteins
... How does this ordered soup of proteins maintain a such a large number of tightly synchronised feedback control systems? ...
... How does this ordered soup of proteins maintain a such a large number of tightly synchronised feedback control systems? ...
Cartoon modeling of proteins
... How does this ordered soup of proteins maintain a such a large number of tightly synchronised feedback control systems? ...
... How does this ordered soup of proteins maintain a such a large number of tightly synchronised feedback control systems? ...
Aquaporin IDI Prelab
... a. Why are the new water channels being developed referred to as biomimetic? ...
... a. Why are the new water channels being developed referred to as biomimetic? ...
What is a Gene?
... • If your hair is brown, it’s because your DNA code is telling your cells to make a specific protein. That protein makes your hair brown. ...
... • If your hair is brown, it’s because your DNA code is telling your cells to make a specific protein. That protein makes your hair brown. ...
Supporting Information File SF5
... guanidine thiocyanate and phenol in mono-phase solution, the thymus tissues were homogenized in TRI REAGENT (1 ml per 50-100 mg of tissue) in a Polytron homogenizer and light vertex and incubation (10 min, room temp). 0.2 ml of chloroform was added per ml of TRI REAGENT and incubation (10 min, room ...
... guanidine thiocyanate and phenol in mono-phase solution, the thymus tissues were homogenized in TRI REAGENT (1 ml per 50-100 mg of tissue) in a Polytron homogenizer and light vertex and incubation (10 min, room temp). 0.2 ml of chloroform was added per ml of TRI REAGENT and incubation (10 min, room ...
SilenciO - PROvendis
... initially only poor expression rate SilenciO-technology is based on a Galectin-1-knock down (>90%) by RNAi. This knock down reduces the metabolic load of the CHO expression system. The expression level of the protein of interest is increased by more than 1.5-fold. ...
... initially only poor expression rate SilenciO-technology is based on a Galectin-1-knock down (>90%) by RNAi. This knock down reduces the metabolic load of the CHO expression system. The expression level of the protein of interest is increased by more than 1.5-fold. ...
File - Biology with Radjewski
... insulin, which has 51 amino acids, to huge molecules such as the muscle protein titin, with 34,350 amino acids. ...
... insulin, which has 51 amino acids, to huge molecules such as the muscle protein titin, with 34,350 amino acids. ...
Surface Display
... Three rounds of panning against streptavidin at constant stringency (0.5% Tween) ...
... Three rounds of panning against streptavidin at constant stringency (0.5% Tween) ...
File - Mrs. LeCompte
... o Disulfide Bridges form between 2 cysteine amino acid monomers brought together by the folding of the protein. ...
... o Disulfide Bridges form between 2 cysteine amino acid monomers brought together by the folding of the protein. ...
Hemagglutinin Protein (HA1 Subunit) (His Tag)
... Western Australia/1756/1983(H15N2)) comprises 342 amino acids and has a predicted molecular mass of 37.6 kDa. The apparent molecular mass of the protein is approximately 51.6 kDa in SDS-PAGE under reducing conditions. ...
... Western Australia/1756/1983(H15N2)) comprises 342 amino acids and has a predicted molecular mass of 37.6 kDa. The apparent molecular mass of the protein is approximately 51.6 kDa in SDS-PAGE under reducing conditions. ...
IN THIS ISSUE Mutating it all Discovering ubiquitylation
... presence of Zn2+. In areas of high Zn2+ concentration ...
... presence of Zn2+. In areas of high Zn2+ concentration ...
生物物理学 I Handout No. 2 ① ② ③ ④ ⑤
... a solute. The carrier protein shown can exist in two conformational states: in state "pong" the binding sites for solute A are exposed on the outside of the bilayer; in state "ping" the same sites are exposed on the other side of the bilayer. The transition between the two states is proposed to occu ...
... a solute. The carrier protein shown can exist in two conformational states: in state "pong" the binding sites for solute A are exposed on the outside of the bilayer; in state "ping" the same sites are exposed on the other side of the bilayer. The transition between the two states is proposed to occu ...
Chapter 10 Intracellular Compartments and Transport
... A common pool of ribosomes is used to synthesize both the proteins that stay in the cytosol and those that are transported into membrane-enclosed organelles, including the ER ...
... A common pool of ribosomes is used to synthesize both the proteins that stay in the cytosol and those that are transported into membrane-enclosed organelles, including the ER ...
medmicro4-weapons delivery – G+
... In most species studied to date R = D-alanine R’ = N-acetylglucosamine In S. pneumoniae and S. suis R = phosphodiester linked choline - chemically more stable than ester-linked D-Ala ...
... In most species studied to date R = D-alanine R’ = N-acetylglucosamine In S. pneumoniae and S. suis R = phosphodiester linked choline - chemically more stable than ester-linked D-Ala ...
Auxiliary proteins of photosystem II: tuning the enzyme for optimal
... was slowed and the PSII-specific electron acceptor 2,5 dimethyl-p-benzoquinone blocked QAoxidation. These effects could be prevented, and in some cases reversed, by the addition of HCO3-, a PS II-specific cofactor that binds to the non-heme iron between QA and QB. In addition, we have obtained the X ...
... was slowed and the PSII-specific electron acceptor 2,5 dimethyl-p-benzoquinone blocked QAoxidation. These effects could be prevented, and in some cases reversed, by the addition of HCO3-, a PS II-specific cofactor that binds to the non-heme iron between QA and QB. In addition, we have obtained the X ...
MEMBRANE STRUCTURE
... MEMBRANE PROTEINS • INTEGRAL or TRANS – MEMBRANE PROTEINS • LIPID-ANCHORED MEMBRANE PROTEINS • PERIPHERAL MEMBRANE PROTEINS ...
... MEMBRANE PROTEINS • INTEGRAL or TRANS – MEMBRANE PROTEINS • LIPID-ANCHORED MEMBRANE PROTEINS • PERIPHERAL MEMBRANE PROTEINS ...
A little less conjugation, a little more accuracy
... this issue focuses on methods to modify proteins in a site-selective manner. Proteins can fold into an incredibly diverse range of structures despite being made from only a limited number of building blocks — the twenty-or-so proteinogenic amino acids. The modular nature of proteins has enabled thei ...
... this issue focuses on methods to modify proteins in a site-selective manner. Proteins can fold into an incredibly diverse range of structures despite being made from only a limited number of building blocks — the twenty-or-so proteinogenic amino acids. The modular nature of proteins has enabled thei ...
Recombinant Human PKA 2 beta (regulatory subunit) protein
... Replacement or refund for products not performing as stated on the datasheet Valid for 12 months from date of delivery Response to your inquiry within 24 hours We provide support in Chinese, English, French, German, Japanese and Spanish Extensive multi-media technical resources to help you We invest ...
... Replacement or refund for products not performing as stated on the datasheet Valid for 12 months from date of delivery Response to your inquiry within 24 hours We provide support in Chinese, English, French, German, Japanese and Spanish Extensive multi-media technical resources to help you We invest ...
The antibody validation experiments were essentially performed as
... 7. The growth medium was removed and 40 µl lysis buffer (150 mM NaCl, 50 mM Tris pH 8, 1% Triton X-100, 0.5% Sodium deoxycholate, 0.1% SDS) was added to each well. The plate was then incubated for 30 min on ice. During the incubation the plate was vortexed ...
... 7. The growth medium was removed and 40 µl lysis buffer (150 mM NaCl, 50 mM Tris pH 8, 1% Triton X-100, 0.5% Sodium deoxycholate, 0.1% SDS) was added to each well. The plate was then incubated for 30 min on ice. During the incubation the plate was vortexed ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.