Genetic Engineering - USF :: Biological Sciences
... Meera Nanjundan, Ph.D Department of Cell Biology, Microbiology, & Molecular Biology •Gene expression, Cloning, & Manipulation of Plasmids •Cell Culture •Transgenics •Mutagenesis •siRNA •Quantitative PCR •Generation of Antibodies •Use of Fluorescent Tags (i.e. GFP) •Protein Expression & Purification ...
... Meera Nanjundan, Ph.D Department of Cell Biology, Microbiology, & Molecular Biology •Gene expression, Cloning, & Manipulation of Plasmids •Cell Culture •Transgenics •Mutagenesis •siRNA •Quantitative PCR •Generation of Antibodies •Use of Fluorescent Tags (i.e. GFP) •Protein Expression & Purification ...
Microbot Preparation
... STREPAVIDIN AND BIOTIN Streptavidin has one of the strongest non-covalent interactions known to chemistry with the vitamin biotin Biotin is a water-soluble B-complex vitamin that can be easily added to antibodies Mechanism: - Biotinylated antibodies latch onto receptor proteins on a bacteria’s surf ...
... STREPAVIDIN AND BIOTIN Streptavidin has one of the strongest non-covalent interactions known to chemistry with the vitamin biotin Biotin is a water-soluble B-complex vitamin that can be easily added to antibodies Mechanism: - Biotinylated antibodies latch onto receptor proteins on a bacteria’s surf ...
We venture into proteins` potential as functional molecules by means
... specifically bind to nano-sized material particles by utilizing the function of antibody proteins that can specifically bind to bacteria and viruses. The antibody protein can act as the “glue in the nanoworld.” Various proteins have come into existence since life first appeared, and 10,000 proteins ...
... specifically bind to nano-sized material particles by utilizing the function of antibody proteins that can specifically bind to bacteria and viruses. The antibody protein can act as the “glue in the nanoworld.” Various proteins have come into existence since life first appeared, and 10,000 proteins ...
LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
... The α and β cyclic forms of D glucose are referred to as__________ The charged amino acid, which is electrically neutral, is called _________. _________ is the specific region on the enzyme at which substrate binds. _________ is the ring system present in cholesterol. Nitrogenous bases are conjugate ...
... The α and β cyclic forms of D glucose are referred to as__________ The charged amino acid, which is electrically neutral, is called _________. _________ is the specific region on the enzyme at which substrate binds. _________ is the ring system present in cholesterol. Nitrogenous bases are conjugate ...
Recombinant Expression of PDI in E. coli
... • Denaure proteins so that they are linear and able to migrate through the gel • Coat with SDS so that all molecules will have a negative charge and will migrate through the gel towards the positive electrode according to size. ...
... • Denaure proteins so that they are linear and able to migrate through the gel • Coat with SDS so that all molecules will have a negative charge and will migrate through the gel towards the positive electrode according to size. ...
Membranes
... • Exocytosis – transport out of cell – Vesicle (membrane package) fuses with plasma membrane, opens, releases outside – E.g. salty tears from tear glands ...
... • Exocytosis – transport out of cell – Vesicle (membrane package) fuses with plasma membrane, opens, releases outside – E.g. salty tears from tear glands ...
Supplementary Information (doc 33K)
... (5’-UUCUCCGAACGUGUCACGU-3’) was used as a control. Corresponding to siRNA sequences for hCINAP and non-silencing, shRNAs were synthesized and inserted into downstream of the U6 promoter in the lentiviral vector pGCSIL-Puromycin (GeneChem Co., Ltd., Shanghai, China). To further verify that the effect ...
... (5’-UUCUCCGAACGUGUCACGU-3’) was used as a control. Corresponding to siRNA sequences for hCINAP and non-silencing, shRNAs were synthesized and inserted into downstream of the U6 promoter in the lentiviral vector pGCSIL-Puromycin (GeneChem Co., Ltd., Shanghai, China). To further verify that the effect ...
ChIP-Chip analysis using GenomPlex-amplified - Sigma
... ChIP-Chip analysis using GenomePlex-amplified Genomic DNA Protein cross-linked with PC12 cell (rat) genomic DNA was isolated by immunoprecipitation, amplified using the GenomePlex WGA2 kits, reamplified with GenomePlex WGA3, and susequently labeled using the Nimblegen Dual-Color labeling kit. Labele ...
... ChIP-Chip analysis using GenomePlex-amplified Genomic DNA Protein cross-linked with PC12 cell (rat) genomic DNA was isolated by immunoprecipitation, amplified using the GenomePlex WGA2 kits, reamplified with GenomePlex WGA3, and susequently labeled using the Nimblegen Dual-Color labeling kit. Labele ...
The Cell Membrane
... 2. Carrier or Transport Proteins- binding site on protein surface "grabs" certain molecules and pulls them into the cell. ...
... 2. Carrier or Transport Proteins- binding site on protein surface "grabs" certain molecules and pulls them into the cell. ...
Protein sequencing by Edman degradation
... acrylamide. Samples may be in solution or bound to a PVDF-membrane. Coomassie staining is tolerated. Proteins larger than 10,000 Da can be desalted here by centrifugation in Prospin cartriges washing out salt, and binding the protein to a PVDF membrane. In order to avoid blocking the α-amino group e ...
... acrylamide. Samples may be in solution or bound to a PVDF-membrane. Coomassie staining is tolerated. Proteins larger than 10,000 Da can be desalted here by centrifugation in Prospin cartriges washing out salt, and binding the protein to a PVDF membrane. In order to avoid blocking the α-amino group e ...
MSH2 (Mismatch Repair Protein 2)
... Staining of formalin-fixed tissue sections requires treating the tissue sections in boiling 10mM citrate buffer, pH 6.0 (Lab Vision catalog # AP-9003), for 10-20 minutes followed by cooling at room temperature for 20 min. ...
... Staining of formalin-fixed tissue sections requires treating the tissue sections in boiling 10mM citrate buffer, pH 6.0 (Lab Vision catalog # AP-9003), for 10-20 minutes followed by cooling at room temperature for 20 min. ...
Bio 263/F94/T2
... d. homogenization 41. Which technique could be used to detect the differences in conformation (shape) seen in enzymes that are bound or unbound to their substrates? a. X-ray diffraction d. isoelectric focussing b. SDS-polyacrylamide gel electrophoresis e. CM-cellulose chromatography c. IR spectromet ...
... d. homogenization 41. Which technique could be used to detect the differences in conformation (shape) seen in enzymes that are bound or unbound to their substrates? a. X-ray diffraction d. isoelectric focussing b. SDS-polyacrylamide gel electrophoresis e. CM-cellulose chromatography c. IR spectromet ...
Protein Structure and Folding
... ways N cysteins can pair with each other. Some proteins like many neutrophic factors contain odd number of cysteins. What kind of pair would you expect in this case? 2. Rearrange equation H - TS = RTln[D]/[N], where [D] is the concentration of the denatured and [N] the native protein, to [D]/([D]+ ...
... ways N cysteins can pair with each other. Some proteins like many neutrophic factors contain odd number of cysteins. What kind of pair would you expect in this case? 2. Rearrange equation H - TS = RTln[D]/[N], where [D] is the concentration of the denatured and [N] the native protein, to [D]/([D]+ ...
Biosynthesis and degradation of proteins
... Protein degradation systems Ubiquitin and proteasome Activation of proteases Protease inhibitors ...
... Protein degradation systems Ubiquitin and proteasome Activation of proteases Protease inhibitors ...
Organic Biomolecules Fill in Notes 2016
... • Only contain the elements carbon, hydrogen, and oxygen in a specific ratio of 1:2:1 Example: formula for glucose is C6H12O6 ...
... • Only contain the elements carbon, hydrogen, and oxygen in a specific ratio of 1:2:1 Example: formula for glucose is C6H12O6 ...
macromolecule_sheets
... 4. What two functional groups are found in monosaccharides? 5. Name three common monosaccharides and give the molecular formula for each. 6. What suffix is commonly found on the end of sugar names? 7. How are monosaccharides used in a cell? 8. What is a disaccharide? Name two common disaccharides. 9 ...
... 4. What two functional groups are found in monosaccharides? 5. Name three common monosaccharides and give the molecular formula for each. 6. What suffix is commonly found on the end of sugar names? 7. How are monosaccharides used in a cell? 8. What is a disaccharide? Name two common disaccharides. 9 ...
E U F T DG Unfolded state, ensemble Native fold, one
... Learning Objectives • After this lesson you should be able to: ...
... Learning Objectives • After this lesson you should be able to: ...
FCS-FS-8. Students will discuss why proteins are important in food
... have a role in both living organisms and in food products ...
... have a role in both living organisms and in food products ...
Protein Structure HW Key
... 16. Discuss how proteins are purified. Depends on the protein, but usually start with some crude source and then a centrifugation step to remove debris. After that, a couple of chromatography steps to purify. 17. What is specific activity? Briefly describe how it is determined. Activity/mg protein. ...
... 16. Discuss how proteins are purified. Depends on the protein, but usually start with some crude source and then a centrifugation step to remove debris. After that, a couple of chromatography steps to purify. 17. What is specific activity? Briefly describe how it is determined. Activity/mg protein. ...
ESBA Go Lean Protein Evaluation
... SNAP-Ed Activity Evaluation Form 00/00/17 with [Educator]: Go Lean with Protein For each statement the middle, please place an “X” in one of the boxes on each side that best represents your perceptions before the workshop (left) and now, after the workshop (right). BEFORE this Workshop Disagree Unsu ...
... SNAP-Ed Activity Evaluation Form 00/00/17 with [Educator]: Go Lean with Protein For each statement the middle, please place an “X” in one of the boxes on each side that best represents your perceptions before the workshop (left) and now, after the workshop (right). BEFORE this Workshop Disagree Unsu ...
Cell Membrane Structure & Function
... • _______________________________ _______________________________ _______________________________ _______________________________ _______________________________ _______________________________ _______________________________ _______________________________ _______________________________ ...
... • _______________________________ _______________________________ _______________________________ _______________________________ _______________________________ _______________________________ _______________________________ _______________________________ _______________________________ ...
Station A 1. Why are polar water molecules attracted to other polar
... 2. Which biomolecule is an enzyme composed of? What are its monomers called? ...
... 2. Which biomolecule is an enzyme composed of? What are its monomers called? ...
Week 5 Assignment 1. Reverse Phase Arrays (RPA) involve printing
... A protein array with purified proteins printed on it belongs to which of the following categories of arrays when probed with serum samples containing autoantibodies? 0.5 points Forward Phase arrays Reverse phase arrays ...
... A protein array with purified proteins printed on it belongs to which of the following categories of arrays when probed with serum samples containing autoantibodies? 0.5 points Forward Phase arrays Reverse phase arrays ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.