Protein Purification and Analysis
... HPLC (high-performance liquid chromatography) Column can be: hydrophobic, (+) or (-) charged, stereospecific, etc. Resin needs to have incompressible beads high pressure pumps speed the movement of proteins down the column HPLC limits protein band spreading - increase resolution ...
... HPLC (high-performance liquid chromatography) Column can be: hydrophobic, (+) or (-) charged, stereospecific, etc. Resin needs to have incompressible beads high pressure pumps speed the movement of proteins down the column HPLC limits protein band spreading - increase resolution ...
No Slide Title
... HPLC (high-performance liquid chromatography) Column can be: hydrophobic, (+) or (-) charged, stereospecific, etc. Resin needs to have incompressible beads high pressure pumps speed the movement of proteins down the column HPLC limits protein band spreading - increase resolution ...
... HPLC (high-performance liquid chromatography) Column can be: hydrophobic, (+) or (-) charged, stereospecific, etc. Resin needs to have incompressible beads high pressure pumps speed the movement of proteins down the column HPLC limits protein band spreading - increase resolution ...
7- Immunological methods
... • In both cases, the tissue is treated to rupture the membranes, usually by using a kind of detergent such as Triton X-100. Dr. Gihan Gawish ...
... • In both cases, the tissue is treated to rupture the membranes, usually by using a kind of detergent such as Triton X-100. Dr. Gihan Gawish ...
CH 6: Proteins and Amino Acids
... a regular/daily basis for proteins to be made – Need all 20 a.a. to make most proteins – Animal sources of proteins contain all 9 essential aa (one exception, gelatin) – Plant sources are missing or low in at least one essential aa (one exception, soy) ...
... a regular/daily basis for proteins to be made – Need all 20 a.a. to make most proteins – Animal sources of proteins contain all 9 essential aa (one exception, gelatin) – Plant sources are missing or low in at least one essential aa (one exception, soy) ...
can detect white spot syndrome virus (wssv
... incubated with hybridoma conditioned media from each culture well (1:20 dilution in 5% Blotto: 5% nonfat drymilk, 0.1% Triton-X-100 in PBS) for 8 h. After extensive washing in 0.5% Blotto, the membrane was incubated in horseradish peroxidase conjugated goat anti-mouse IgG heavy and light chain speci ...
... incubated with hybridoma conditioned media from each culture well (1:20 dilution in 5% Blotto: 5% nonfat drymilk, 0.1% Triton-X-100 in PBS) for 8 h. After extensive washing in 0.5% Blotto, the membrane was incubated in horseradish peroxidase conjugated goat anti-mouse IgG heavy and light chain speci ...
lecture5lifes_chemical_basis
... Biochemistry because it was demonstrated that the conformation of a polypeptide chain can be predicted if the properties of its constituents are rigorously and precisely known. For this work Pauling got the Nobel prize in Chemistry in 1954. The helical content of a protein may vary anywhere between ...
... Biochemistry because it was demonstrated that the conformation of a polypeptide chain can be predicted if the properties of its constituents are rigorously and precisely known. For this work Pauling got the Nobel prize in Chemistry in 1954. The helical content of a protein may vary anywhere between ...
Chapter 2 slides
... • There are approximately 100 billion neurons in the human brain • There are about 100 times as many glial cells in the human brain • Similar origin, different functions • Other cells include ependymal cells, microglia and cells of the brain vasculature ...
... • There are approximately 100 billion neurons in the human brain • There are about 100 times as many glial cells in the human brain • Similar origin, different functions • Other cells include ependymal cells, microglia and cells of the brain vasculature ...
Biological Membranes
... bond creates a kink in the fatty acyl tail, making it more difficult for neighboring tails to interact and making the bilayer more fluid ...
... bond creates a kink in the fatty acyl tail, making it more difficult for neighboring tails to interact and making the bilayer more fluid ...
Figure S1. - BioMed Central
... Figure S9. Detailed information on Toll-like receptor (TLR) family (A) Diagram of the putative Toll-like receptor 13 dimer. The putative model of Tlr13 was constructed based on domain prediction and the crystal structure of other TLRs (refer to supplementary text). The Nglycosylation sites in our s ...
... Figure S9. Detailed information on Toll-like receptor (TLR) family (A) Diagram of the putative Toll-like receptor 13 dimer. The putative model of Tlr13 was constructed based on domain prediction and the crystal structure of other TLRs (refer to supplementary text). The Nglycosylation sites in our s ...
The Post-Game/Practice Meal
... Rehydration is also an important part of recovery after exercise. In general, 16-24 oz of fluid should be consumed for every pound lost during exercise. For some athletes, drinking a sports drink that contains carbohydrates and electrolytes, such as sodium, can be beneficial after exercise. Sodium h ...
... Rehydration is also an important part of recovery after exercise. In general, 16-24 oz of fluid should be consumed for every pound lost during exercise. For some athletes, drinking a sports drink that contains carbohydrates and electrolytes, such as sodium, can be beneficial after exercise. Sodium h ...
Document
... • Cats are specifically adapted for a high protein, low carb diet. They depend on gluconeogenesis as a major source of energy. Cat’s are limited in their ability to conserve protein due to continuous protein catabolism. • Metabolism of excess amino acids increases liver and kidney overload. ...
... • Cats are specifically adapted for a high protein, low carb diet. They depend on gluconeogenesis as a major source of energy. Cat’s are limited in their ability to conserve protein due to continuous protein catabolism. • Metabolism of excess amino acids increases liver and kidney overload. ...
Bioproduction of recombinant protein
... The customized cell lines can be constructed both for validation of candidate genes and recombinant protein production. The gene transfer using lentiviral methods has two main benefits: > stable cell lines are established in a very short time, ...
... The customized cell lines can be constructed both for validation of candidate genes and recombinant protein production. The gene transfer using lentiviral methods has two main benefits: > stable cell lines are established in a very short time, ...
F212 2.1.1 Biological Molecules Proteins
... • Biuret is made up of sodium hydroxide and copper sulphate, when these are added to protein they will turn lilac in colour. • Which solution contains proteins? ...
... • Biuret is made up of sodium hydroxide and copper sulphate, when these are added to protein they will turn lilac in colour. • Which solution contains proteins? ...
chapter 7 cell membrane
... Controls traffic into & out of the cell selectively permeable allowing some substances to cross more easily than others ...
... Controls traffic into & out of the cell selectively permeable allowing some substances to cross more easily than others ...
Serum Total Protein
... – Polyclonal increase • Only disorders affecting the concentration of albumin and/or the immunoglobulins will give rise to abnormal total protein levels. • Other serum proteins are never present in high enough concentrations for changes to have a significant overall effect. ...
... – Polyclonal increase • Only disorders affecting the concentration of albumin and/or the immunoglobulins will give rise to abnormal total protein levels. • Other serum proteins are never present in high enough concentrations for changes to have a significant overall effect. ...
Rat LIFR Protein (His Tag)
... 1. Measured by its ability to bind mouse LIF-Fc (Cat:50755-M02H) in a functional ELISA. 2. Measured by its ability to inhibit the recombinant human LIF mediated inhibition in the M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 8-40 ng/mL in the presence of 2 ng/mL recombinant ...
... 1. Measured by its ability to bind mouse LIF-Fc (Cat:50755-M02H) in a functional ELISA. 2. Measured by its ability to inhibit the recombinant human LIF mediated inhibition in the M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 8-40 ng/mL in the presence of 2 ng/mL recombinant ...
Coffee: More Than Just a Jolt in the Morning
... Some proponents of plant protein note that if all the agricultural land that is used for livestock was switched to growing grains, the world could feed several times as many people as are fed now (5). Although this switch would create a surplus of food there are still other reasons for switching the ...
... Some proponents of plant protein note that if all the agricultural land that is used for livestock was switched to growing grains, the world could feed several times as many people as are fed now (5). Although this switch would create a surplus of food there are still other reasons for switching the ...
Cells are exposed to DNA damaging agents that can affect their
... In the recent years, our group has been working on the challenge of using single-particle EM to build 3D reconstructions of small and/or asymmetric macromolecules (1-5). These are important steps to solve because most of the molecules of interest in biology do not have any symmetry, especially those ...
... In the recent years, our group has been working on the challenge of using single-particle EM to build 3D reconstructions of small and/or asymmetric macromolecules (1-5). These are important steps to solve because most of the molecules of interest in biology do not have any symmetry, especially those ...
Nutrients
... • Location of Protein in feed material • The "germ" is the part of the grain kernel which usually contains available protein. ...
... • Location of Protein in feed material • The "germ" is the part of the grain kernel which usually contains available protein. ...
Anti-HSP70 Catalog# SPC- 1 78C/D Size: 25/100µg This product is
... expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the Cterminal half (3). The structure of this ATP binding domain displays mult ...
... expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the Cterminal half (3). The structure of this ATP binding domain displays mult ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.