1 - 嘉義大學
... thousand times greater than the Km for that substrate. After 9 minutes, 1% of the substrate had been converted to product, and the amount of product formed in the reaction mixture was 12 mol. If, in a separate experiment, one-third as much enzyme and twice as much substrate had been combined, how l ...
... thousand times greater than the Km for that substrate. After 9 minutes, 1% of the substrate had been converted to product, and the amount of product formed in the reaction mixture was 12 mol. If, in a separate experiment, one-third as much enzyme and twice as much substrate had been combined, how l ...
2010 Protein Metabolism I
... • Free amino acids, nucleic acids, amines, ammonia, nitrates, nitrites, urea ...
... • Free amino acids, nucleic acids, amines, ammonia, nitrates, nitrites, urea ...
Protein Kinases Structural Features
... for nucleophilic attack Activation Loop (DFG….p(S/T/Y)….APE) – The Asp group interacts with Mg for positioning Often contains a phosphorylation site that upon phosphorylation induces a conformational change on the loop that allows substrate to bind and also positions the catalytic Asp group of the c ...
... for nucleophilic attack Activation Loop (DFG….p(S/T/Y)….APE) – The Asp group interacts with Mg for positioning Often contains a phosphorylation site that upon phosphorylation induces a conformational change on the loop that allows substrate to bind and also positions the catalytic Asp group of the c ...
Molecules of Life
... • Enzymes are catalytic molecules • They speed the rate at which reactions approach equilibrium ...
... • Enzymes are catalytic molecules • They speed the rate at which reactions approach equilibrium ...
College 1 - Xray and NMR
... 2. ABPP probe is added and they only bind to active proteases, so none that are inhibited. 3. Active proteases, on which an ABPP probe is bound, become fluorescent. 4. Through SDS-PAGE it can be checked which proteases are active and which are not. ...
... 2. ABPP probe is added and they only bind to active proteases, so none that are inhibited. 3. Active proteases, on which an ABPP probe is bound, become fluorescent. 4. Through SDS-PAGE it can be checked which proteases are active and which are not. ...
NNI086 - Nestlé Nutrition Institute
... amino acids combine to form the proteins. Some amino acids are essential dietary components, since they are not synthetized by human metabolic processes. Proteins are present in and vital to every living cell. They are essential for healthy growth and development and also influence major functions o ...
... amino acids combine to form the proteins. Some amino acids are essential dietary components, since they are not synthetized by human metabolic processes. Proteins are present in and vital to every living cell. They are essential for healthy growth and development and also influence major functions o ...
All the following is correct about ribosomes EXCEPT
... Carbon chains form the skeletons of most organic molecules, the skeletons may be a. straight only b. branched only c. arranged in closed rings only d. All of them ...
... Carbon chains form the skeletons of most organic molecules, the skeletons may be a. straight only b. branched only c. arranged in closed rings only d. All of them ...
ELECTROPHORESIS
... Polyacrylamide Gel Electrophoresis (PAGE) Used routinely in the analysis of single stranded and double stranded DNA. Polyacrylamide is cross linked with TEMED to form a porous gel, thus allowing movement of DNA molecules. Separation of DNA is based on size. For example DNA bands made of 1000-2000 ba ...
... Polyacrylamide Gel Electrophoresis (PAGE) Used routinely in the analysis of single stranded and double stranded DNA. Polyacrylamide is cross linked with TEMED to form a porous gel, thus allowing movement of DNA molecules. Separation of DNA is based on size. For example DNA bands made of 1000-2000 ba ...
SafeView - NBS Biologicals
... Staining solution may be stored at room temperature and reused. SafeView is non-carcinogenic but may cause skin and eye irritations. Always wear gloves when working with the product. This product is distributed for laboratory research only. CAUTION: Not for diagnostic use. The safety and efficiency ...
... Staining solution may be stored at room temperature and reused. SafeView is non-carcinogenic but may cause skin and eye irritations. Always wear gloves when working with the product. This product is distributed for laboratory research only. CAUTION: Not for diagnostic use. The safety and efficiency ...
Chapter 12. Protein biosynthesis (P215, sP875)
... monocistronic: one mRNA encodes only a single polypeptide chain. mRNA in prokaryotes usually encodes more than one polypeptide chain. This is called polycistronic. ...
... monocistronic: one mRNA encodes only a single polypeptide chain. mRNA in prokaryotes usually encodes more than one polypeptide chain. This is called polycistronic. ...
Unknown function, JCSG
... belong to the phosphorylase/hydrolase-like fold in SCOP. These proteins show significant sequence divergence and have a broad phylogenetic distribution across both prokaryotes and eukaryotes. At the time, despite 40 unique experimentally determined structures in the PDB, only half of the family memb ...
... belong to the phosphorylase/hydrolase-like fold in SCOP. These proteins show significant sequence divergence and have a broad phylogenetic distribution across both prokaryotes and eukaryotes. At the time, despite 40 unique experimentally determined structures in the PDB, only half of the family memb ...
Reticulons (RTNs) are endomembrane bound proteins with a
... Reticulons (RTNs) gained increasing attention in the recent years due to their involvement and crucial role especially in neurodegenerative diseases. RTNs are endomembrane bound proteins with a uniquely conserved C-terminal Reticulon homology domain (RHD), via which they anchor themselves to membran ...
... Reticulons (RTNs) gained increasing attention in the recent years due to their involvement and crucial role especially in neurodegenerative diseases. RTNs are endomembrane bound proteins with a uniquely conserved C-terminal Reticulon homology domain (RHD), via which they anchor themselves to membran ...
protein - mustafaaltinisik.org.uk
... Most of the 20 common amino acids found in proteins were discovered. 1864 Hoppe-Seyler crystallized, and named, the protein hemoglobin. 1894 Fischer proposed a lock-and-key analogy for enzyme-substrate interactions. 1897 Buchner and Buchner showed that cell-free extracts of yeast can ferment sucrose ...
... Most of the 20 common amino acids found in proteins were discovered. 1864 Hoppe-Seyler crystallized, and named, the protein hemoglobin. 1894 Fischer proposed a lock-and-key analogy for enzyme-substrate interactions. 1897 Buchner and Buchner showed that cell-free extracts of yeast can ferment sucrose ...
Sample exam 1
... elements of secondary structure like alpha helices and beta-pleated sheets. In the context of secondary structures and how they relate to the protein’s function, explain why this makes sense. 8. From the following data on an enzymatic reaction, determine (a) the type of inhibition, (b) KM for the su ...
... elements of secondary structure like alpha helices and beta-pleated sheets. In the context of secondary structures and how they relate to the protein’s function, explain why this makes sense. 8. From the following data on an enzymatic reaction, determine (a) the type of inhibition, (b) KM for the su ...
Final Exam: Multiple Choice Portion Biochem Block Spring 2016
... 23. (3 pts) Oral contraceptives for women resemble which molecule below? A) ATP B) pyruvate C) cholesterol D) oleate 24. Which statement describes best what happens in glycolysis? A) fructose is cleaved into two molecules of guanine, absorbing energy B) glucose is cleaved into two molecules of pyru ...
... 23. (3 pts) Oral contraceptives for women resemble which molecule below? A) ATP B) pyruvate C) cholesterol D) oleate 24. Which statement describes best what happens in glycolysis? A) fructose is cleaved into two molecules of guanine, absorbing energy B) glucose is cleaved into two molecules of pyru ...
Chlamydomonas reinhardtii strains carrying the stb1-1
... Chlamydomonas reinhardtii wild-type strain 137C was grown under 12h-light/12h-dark cycle with 2 % (v/v) CO2 constant bubbling during 4 days. Cells were then collected every three hours and crude extracts were prepared from each sample. Half of each samples was used to extract starch. The starch cont ...
... Chlamydomonas reinhardtii wild-type strain 137C was grown under 12h-light/12h-dark cycle with 2 % (v/v) CO2 constant bubbling during 4 days. Cells were then collected every three hours and crude extracts were prepared from each sample. Half of each samples was used to extract starch. The starch cont ...
Study Guide Questions Midterm 2
... 1. What are proteins made up of? What are the differences among essential, non-‐essential, and conditionally essential amino acids? 2. Proteins are linked by what type of bond? 3. Name specific functio ...
... 1. What are proteins made up of? What are the differences among essential, non-‐essential, and conditionally essential amino acids? 2. Proteins are linked by what type of bond? 3. Name specific functio ...
Colorimetric Methods for Determining Protein Concentration. Goals
... based or Coomassie blue binding. • Protein denaturation in these methods is absolutely required in order to get maximal ...
... based or Coomassie blue binding. • Protein denaturation in these methods is absolutely required in order to get maximal ...
A 27-year-old man presents to the outpatient clinic complaining of 2
... The next diagnostic step to would be to obtain the following lab tests: blood cultures x2 to identify if the infection is streptococcol. We would get a rapid strep test as well as throat culture to see if the initial sore throat 2 weeks ago may have been strep. We would obtain an antistreptolysin-O ...
... The next diagnostic step to would be to obtain the following lab tests: blood cultures x2 to identify if the infection is streptococcol. We would get a rapid strep test as well as throat culture to see if the initial sore throat 2 weeks ago may have been strep. We would obtain an antistreptolysin-O ...
How does prolyl hydroxylase-3 induce apoptosis in neuronal
... antibodies were used, in addition to the Novus Biological PHD3 antibody. However, multiple bands were also present, questioning the specificity of the antibody. In addition, the higher molecular weight bands detected with the various antibodies did not correspond to each other, suggesting that they ...
... antibodies were used, in addition to the Novus Biological PHD3 antibody. However, multiple bands were also present, questioning the specificity of the antibody. In addition, the higher molecular weight bands detected with the various antibodies did not correspond to each other, suggesting that they ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.