Silk and its Biosynthesis in Silkworm Bombyx mori
... Sericin: Sericin is a hot water-soluble macromolecular globular protein with molecular mass of 10-310 kDa, cements the fibroin fibre that helps in the formation of cocoon. Sericin contributes about 20-30% of the total cocoon weight. Sericin is made up of 18 amino acids, most having strongly polar si ...
... Sericin: Sericin is a hot water-soluble macromolecular globular protein with molecular mass of 10-310 kDa, cements the fibroin fibre that helps in the formation of cocoon. Sericin contributes about 20-30% of the total cocoon weight. Sericin is made up of 18 amino acids, most having strongly polar si ...
Intestinal absorption of amino acids and peptides
... Competition for intestinal transport and transport groups T h e results of animal experiments, in particular experiments on competition for intestinal transport (usually in rat or hamster), supported by observations on intestinal transport defects in man, have suggested the existence of several ‘tra ...
... Competition for intestinal transport and transport groups T h e results of animal experiments, in particular experiments on competition for intestinal transport (usually in rat or hamster), supported by observations on intestinal transport defects in man, have suggested the existence of several ‘tra ...
Nitrate Reductases: Structure, Functions, and Effect of Stress Factors
... been supposed recently that only high-potential clusters are able, for thermodynamic reasons, to transfer electrons, whereas the role of the other two clusters is still unknown. Subunit α (104-150 kD) coded by the narG gene contains the [4Fe-4S] cluster and molybdenum cofactor, just on which the nit ...
... been supposed recently that only high-potential clusters are able, for thermodynamic reasons, to transfer electrons, whereas the role of the other two clusters is still unknown. Subunit α (104-150 kD) coded by the narG gene contains the [4Fe-4S] cluster and molybdenum cofactor, just on which the nit ...
STRONG AND WEAK HYDROGEN BONDS IN Sm/LSm
... unsaturation in the bonds. Weak interactions play a modest individual influence on chemical structures, however their cumulative effect can be profound and has a large influence on the conformational stability of a biomolecule [13,14]. What contributes to the stability of protein oligomers is the de ...
... unsaturation in the bonds. Weak interactions play a modest individual influence on chemical structures, however their cumulative effect can be profound and has a large influence on the conformational stability of a biomolecule [13,14]. What contributes to the stability of protein oligomers is the de ...
LABORATORY MANUAL ON BIOLOGICAL CHEMISTRY
... From 1995 to 1998, the head of biochemistry unit was prof. M.F. Tymochko. The main goal of his scientific research was the investigation of the fundamentals of adaptive-compensatory processes under various experimental conditions with emphasize on changes in oxygen-dependent reactions. He studied me ...
... From 1995 to 1998, the head of biochemistry unit was prof. M.F. Tymochko. The main goal of his scientific research was the investigation of the fundamentals of adaptive-compensatory processes under various experimental conditions with emphasize on changes in oxygen-dependent reactions. He studied me ...
pdf file
... generated by glutamate + malate addition (in the absence of nigericin) was much lower than that measured with succinate (see the fluorescence intensity scale in Fig. 3A and B). Again AEA delayed the rebuilding of the potential which was almost completely collapsed by ADP addition at the steady-state ...
... generated by glutamate + malate addition (in the absence of nigericin) was much lower than that measured with succinate (see the fluorescence intensity scale in Fig. 3A and B). Again AEA delayed the rebuilding of the potential which was almost completely collapsed by ADP addition at the steady-state ...
Document
... •List and discuss four forces that stabilize globular protein structure. •List important structural similarities and differences between myoglobin and hemoglobin. •Describe the mutation present in hemoglobin giving rise to sickle cell disease. ...
... •List and discuss four forces that stabilize globular protein structure. •List important structural similarities and differences between myoglobin and hemoglobin. •Describe the mutation present in hemoglobin giving rise to sickle cell disease. ...
Alpha and beta subunits of pyruvate dehydrogenase E1
... nearly all mitochondriate eukaryotes, this complex oxidatively decarboxylates pyruvate as it enters the mitochondrion, resulting in carbon dioxide, NADH, and acetyl-CoA, the latter of which then enters the Krebs cycle. E1 is a heterodimeric enzyme which begins this series of reactions by converting ...
... nearly all mitochondriate eukaryotes, this complex oxidatively decarboxylates pyruvate as it enters the mitochondrion, resulting in carbon dioxide, NADH, and acetyl-CoA, the latter of which then enters the Krebs cycle. E1 is a heterodimeric enzyme which begins this series of reactions by converting ...
Control of grape berry development
... You can change this image to be appropriate for your topic by inserting an image in this space or use the alternate title slide with lines. Note: only one image should be used and do not overlap the title text. Enter your Business Unit or Flagship name in the ribbon above the url. Add collaborator l ...
... You can change this image to be appropriate for your topic by inserting an image in this space or use the alternate title slide with lines. Note: only one image should be used and do not overlap the title text. Enter your Business Unit or Flagship name in the ribbon above the url. Add collaborator l ...
Purification and Characterization of a Novel Pumpkin Short
... SDS-PAGE and western blot employing the antiserum raised against the Arabidopsis short-chain ACOX. Since the Arabidopsis short-chain ACOX shares high homology (up to 37%) at the amino acid level with mammalian ACDH, it would be interesting to detect some cross-reactivity with mitochondrial proteins ...
... SDS-PAGE and western blot employing the antiserum raised against the Arabidopsis short-chain ACOX. Since the Arabidopsis short-chain ACOX shares high homology (up to 37%) at the amino acid level with mammalian ACDH, it would be interesting to detect some cross-reactivity with mitochondrial proteins ...
Mitochondrial Membrane Potential in Cardiac
... concentrations, J-aggregates form and emit red light, whereas at low concentrations the monomer form emits green light (Griffiths 2000). Several criteria must be met for these probes to be useful in the estimation of MMP: they should not cause loss of mitochondrial function and/or depletion of MMP a ...
... concentrations, J-aggregates form and emit red light, whereas at low concentrations the monomer form emits green light (Griffiths 2000). Several criteria must be met for these probes to be useful in the estimation of MMP: they should not cause loss of mitochondrial function and/or depletion of MMP a ...
Modulating the cobalt redox potential through imidazole hydrogen
... and 1-H+ (375 vs. 150 Hz) is due to the distinct nuclear quadrupolar properties of 59Co, which signicantly increase the t1 and t2 relaxation rates of the directly bound carbon. These effects also prevent the observation of resolved 13C-multiplets due to scalar coupling between 13C and 59Co. Other br ...
... and 1-H+ (375 vs. 150 Hz) is due to the distinct nuclear quadrupolar properties of 59Co, which signicantly increase the t1 and t2 relaxation rates of the directly bound carbon. These effects also prevent the observation of resolved 13C-multiplets due to scalar coupling between 13C and 59Co. Other br ...
Identification of Bioactive Peptide Sequences from Amaranth
... From the whole grain, tasteful soups, stews, sauces, porridges, and soufflés can be prepared. Boiled grains can be used like rice and couscous, which is traditionally made with semolina of wheat. When amaranth grains are boiled, the starch is leaching out and is gelatinized. This causes the cooking ...
... From the whole grain, tasteful soups, stews, sauces, porridges, and soufflés can be prepared. Boiled grains can be used like rice and couscous, which is traditionally made with semolina of wheat. When amaranth grains are boiled, the starch is leaching out and is gelatinized. This causes the cooking ...
Enzyme - MACscience
... This is called the active site. The active site is usually a cleft or pocket at the surface of the enzyme. Substrate modification occurs at the active site. Enzymes are substrate-specific, although specificity varies from enzyme to enzyme: High specificity: The enzyme will only bind with a single ty ...
... This is called the active site. The active site is usually a cleft or pocket at the surface of the enzyme. Substrate modification occurs at the active site. Enzymes are substrate-specific, although specificity varies from enzyme to enzyme: High specificity: The enzyme will only bind with a single ty ...
Chapter 8: Fibrous Proteins
... Fibrous proteins are highly elongated polypeptides composed of a single secondary structure element Primary component of skin, tendon, bone, connective tissues, etc Function as structural material that have protective, connective or supportive roles Simplicity of structure makes relation between str ...
... Fibrous proteins are highly elongated polypeptides composed of a single secondary structure element Primary component of skin, tendon, bone, connective tissues, etc Function as structural material that have protective, connective or supportive roles Simplicity of structure makes relation between str ...
4. characterisation of novel proteins
... Refined soybean oil from MON87769 contains 20-30% SDA (% total fatty acids), and 5-8% gamma-linolenic acid (GLA), neither of which is present in conventional soybean oil. SDA soybean oil also contains slightly higher levels of alpha-linolenic acid (ALA) and palmitic acid as well as lower levels of o ...
... Refined soybean oil from MON87769 contains 20-30% SDA (% total fatty acids), and 5-8% gamma-linolenic acid (GLA), neither of which is present in conventional soybean oil. SDA soybean oil also contains slightly higher levels of alpha-linolenic acid (ALA) and palmitic acid as well as lower levels of o ...
Food and Fluid Guidelines Before, During, and After Exercise
... form of glucose as blood sugar. A workout that is of moderate intensity draws about half of the energy demand from muscle glycogen and blood glucose, and as exercise intensity increases, about two-thirds of needed energy comes from these 2 sources. Liver glycogen is used to maintain blood sugar leve ...
... form of glucose as blood sugar. A workout that is of moderate intensity draws about half of the energy demand from muscle glycogen and blood glucose, and as exercise intensity increases, about two-thirds of needed energy comes from these 2 sources. Liver glycogen is used to maintain blood sugar leve ...
The Role of Cyclin B in Meiosis I
... Adult clams (Spisula solidissima) were collected by the Marine Resources staff of the Marine Biological Laboratory, Woods Hole, MA, and maintained in sea water at 14-18°C for up to several months. Oocytes were collected by dissection (Rosenthal et al., 1980), settled 3-5 times at room temperature in ...
... Adult clams (Spisula solidissima) were collected by the Marine Resources staff of the Marine Biological Laboratory, Woods Hole, MA, and maintained in sea water at 14-18°C for up to several months. Oocytes were collected by dissection (Rosenthal et al., 1980), settled 3-5 times at room temperature in ...
AP Biology Cell Unit Exam - Speedway High School
... 60) Which of the following are directly associated with photosystem I? A) extraction of hydrogen electrons from the splitting of water B) generation of molecular oxygen C) passing electrons to the thylakoid membrane electron transport chain D) harvesting of light energy by ATP E) receiving electron ...
... 60) Which of the following are directly associated with photosystem I? A) extraction of hydrogen electrons from the splitting of water B) generation of molecular oxygen C) passing electrons to the thylakoid membrane electron transport chain D) harvesting of light energy by ATP E) receiving electron ...
Identification of a Cytoplasm to Vacuole Targeting Determinant in
... series of vesicle-mediated transport steps, the proteins are carried from the ER through the subcompartments of the Golgi before being directed to the vacuole (reviewed in Pryer et al., 1992). Transit through this portion of the ...
... series of vesicle-mediated transport steps, the proteins are carried from the ER through the subcompartments of the Golgi before being directed to the vacuole (reviewed in Pryer et al., 1992). Transit through this portion of the ...
Exam_2005 - The University of Sydney
... 1 kg of human tissue, on average, contains somewhere between 0.5 and 5 mg ATP In a healthy cell, the [ATP] is always much less than the [ADP] The total adenine nucleotide pool ([ATP] + [ADP] + [AMP]) in cells is about 5 mM ATP can be produced in the mitochondria of liver cells and transported in the ...
... 1 kg of human tissue, on average, contains somewhere between 0.5 and 5 mg ATP In a healthy cell, the [ATP] is always much less than the [ADP] The total adenine nucleotide pool ([ATP] + [ADP] + [AMP]) in cells is about 5 mM ATP can be produced in the mitochondria of liver cells and transported in the ...
Amino Acid Analysis Amino acid analysis refers to the methodology
... Hydrolysis of protein and peptide samples is necessary for amino acid analysis of these molecules. The glassware used for hydrolysis must be very clean to avoid erroneous results. Glove powders and fingerprints on hydrolysis tubes may cause contamination. To clean glass hydrolysis tubes, boil tubes ...
... Hydrolysis of protein and peptide samples is necessary for amino acid analysis of these molecules. The glassware used for hydrolysis must be very clean to avoid erroneous results. Glove powders and fingerprints on hydrolysis tubes may cause contamination. To clean glass hydrolysis tubes, boil tubes ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.