Pepsinogen and Pepsin - The Journal of General Physiology
... T h e forces tending to hold the folds of proteins together in some specific fashion include hydrogen and hydrophobic bonds, electrostatic and disulfide bonds (33). The last are the strongest, but there are only three per molecule of pepsinogen, whereas the hydrogen and hydrophobic bonds are more nu ...
... T h e forces tending to hold the folds of proteins together in some specific fashion include hydrogen and hydrophobic bonds, electrostatic and disulfide bonds (33). The last are the strongest, but there are only three per molecule of pepsinogen, whereas the hydrogen and hydrophobic bonds are more nu ...
Molecular cloning of Per a 1 and definition of the cross
... allergens. However, the specificity of their mAb was not known, and the relationship of the sequences to other cockroach allergens could not be established. Taking our data in conjunction with that of Wu et al25 and Helm et al,15 it is clear that the Cr-PII sequences and Bla g Bd90K belong to the sa ...
... allergens. However, the specificity of their mAb was not known, and the relationship of the sequences to other cockroach allergens could not be established. Taking our data in conjunction with that of Wu et al25 and Helm et al,15 it is clear that the Cr-PII sequences and Bla g Bd90K belong to the sa ...
Fixation
... when samples are prepared for electron microscopy. (It is not used for light microscopy ...
... when samples are prepared for electron microscopy. (It is not used for light microscopy ...
Fixation
... when samples are prepared for electron microscopy. (It is not used for light microscopy ...
... when samples are prepared for electron microscopy. (It is not used for light microscopy ...
Assessing in silico the recruitment and functional spectrum of
... means of specific biosynthetic pathways and the corresponding genes are organized in biosynthetic gene clusters (BGCs) [5]. Compared to products of PM, secondary metabolites have a wider range of structures and biological activities [6]. This remarkable diversity reflects the random manner in which ...
... means of specific biosynthetic pathways and the corresponding genes are organized in biosynthetic gene clusters (BGCs) [5]. Compared to products of PM, secondary metabolites have a wider range of structures and biological activities [6]. This remarkable diversity reflects the random manner in which ...
Adjeitey_Cyril _Nii-Klu_2013_ thesis
... protonmotive force formed across the mitochondrial inner membrane as heat. It is hypothesized that proton leaks through UCP1 could be a molecular target to combat certain forms of obesity. Although it is well established that UCP1 is regulated by allosteric mechanisms, alternative methods such as po ...
... protonmotive force formed across the mitochondrial inner membrane as heat. It is hypothesized that proton leaks through UCP1 could be a molecular target to combat certain forms of obesity. Although it is well established that UCP1 is regulated by allosteric mechanisms, alternative methods such as po ...
Ribosomes of Mouse Liver following
... the procedure for separating free and membrane-bound ribosomes was modified to give more complete sedimenta tion of the free particles (cf. Chart 2 and Table 3). The distribution experiment was also performed with labeled total free ribosomes in which 7% of the counts were as monomers. In this case, ...
... the procedure for separating free and membrane-bound ribosomes was modified to give more complete sedimenta tion of the free particles (cf. Chart 2 and Table 3). The distribution experiment was also performed with labeled total free ribosomes in which 7% of the counts were as monomers. In this case, ...
Kinetic mechanism of the dimeric ATP sulfurylase from plants
... described [10]. Transformed Escherichia coli BL21(DE3) were grown at 37 ◦ C in Terrific broth containing 50 μg ml − 1 kanamycin until A600nm ∼ 0.6–0.9. Following induction with 1 mM isopropyl 1-thio-β-D-galactopyranoside, cell cultures were incubated overnight (20 ◦ C; 250 rev./min). Cell pellets we ...
... described [10]. Transformed Escherichia coli BL21(DE3) were grown at 37 ◦ C in Terrific broth containing 50 μg ml − 1 kanamycin until A600nm ∼ 0.6–0.9. Following induction with 1 mM isopropyl 1-thio-β-D-galactopyranoside, cell cultures were incubated overnight (20 ◦ C; 250 rev./min). Cell pellets we ...
Predicting Protein Stability Changes upon Mutation Using Database
... of interactions. Local interactions along the chain are described by torsion potentials, based on propensities of amino acids to be associated with backbone torsion angle domains. Non-local interactions along the sequence are represented by distance potentials, derived from propensities of amino aci ...
... of interactions. Local interactions along the chain are described by torsion potentials, based on propensities of amino acids to be associated with backbone torsion angle domains. Non-local interactions along the sequence are represented by distance potentials, derived from propensities of amino aci ...
Leukaemia Section t(4;17)(q12;q21) Atlas of Genetics and Cytogenetics in Oncology and Haematology
... formation of chimeric homodimers and thereby removing requirement of RXR for RARA to bind DNA. The homodimerization ability of RARA fusion proteins is critical for leukemic transformation. Recently, it was shown in a murine system that retroviral transduced FIP1L1/PDGFRA mediated transformation in v ...
... formation of chimeric homodimers and thereby removing requirement of RXR for RARA to bind DNA. The homodimerization ability of RARA fusion proteins is critical for leukemic transformation. Recently, it was shown in a murine system that retroviral transduced FIP1L1/PDGFRA mediated transformation in v ...
hotspots
... • Knowledge based method developed to identify interface residues from sequence • Based on system of neural networks • Takes as input: - sequence environment of residues - evolutionary profile of residues in the window - predicted solvent accessibility - predicted secondary structure - predicted con ...
... • Knowledge based method developed to identify interface residues from sequence • Based on system of neural networks • Takes as input: - sequence environment of residues - evolutionary profile of residues in the window - predicted solvent accessibility - predicted secondary structure - predicted con ...
Understanding the acetylome: translating targeted
... demonstrated that metabolic flux profiles showed distinct patterns, depending on the substrate and level of acetylation. When the carbon source was citrate, metabolism bypassed glyoxylate and metabolism shifted to a gluconeogenic-predominant pathway in coordination with increased acetylation. Collec ...
... demonstrated that metabolic flux profiles showed distinct patterns, depending on the substrate and level of acetylation. When the carbon source was citrate, metabolism bypassed glyoxylate and metabolism shifted to a gluconeogenic-predominant pathway in coordination with increased acetylation. Collec ...
A Molecular Basis for Multiple Herbicide Resistance in Black
... In 1999 the Edwards lab identified AmGSTF1, constitutively expressed in MHR black-grass but not in herbicide sensitive black-grass or target site ...
... In 1999 the Edwards lab identified AmGSTF1, constitutively expressed in MHR black-grass but not in herbicide sensitive black-grass or target site ...
2.3.3 Protein and amino acid metabolism
... glycolytic origin. Its amino group arises, via transamination, from other amino acids, principally the branched-chain amino acids. This alanine is converted to glucose by the liver. This ‘glucose–alanine cycle’ plays a major role in ferrying these amino groups to the liver for detoxification, withou ...
... glycolytic origin. Its amino group arises, via transamination, from other amino acids, principally the branched-chain amino acids. This alanine is converted to glucose by the liver. This ‘glucose–alanine cycle’ plays a major role in ferrying these amino groups to the liver for detoxification, withou ...
phospholipids
... • COX-2 levels increase in inflammatory disease states such as arthritis and cancer • Up-regulation of COX-2 is responsible for the increased formation of prostaglandins associated with ...
... • COX-2 levels increase in inflammatory disease states such as arthritis and cancer • Up-regulation of COX-2 is responsible for the increased formation of prostaglandins associated with ...
Regulation of Cytochrome bd Expression in Mycobacterium
... Rhodococcus and Nocardia have related properties. There are over 100 species that belong to the genus Mycobacterium and 16S rRNA sequence analyses reveals that all mycobacterial species are closely related (143, 159). Mycobacteria have been found to be widely distributed in the environment and most ...
... Rhodococcus and Nocardia have related properties. There are over 100 species that belong to the genus Mycobacterium and 16S rRNA sequence analyses reveals that all mycobacterial species are closely related (143, 159). Mycobacteria have been found to be widely distributed in the environment and most ...
(EC 2.2.1.1) active in the Calvin cycle of spinach chloroplasts
... triosephosphate isomerase, and 40 #1 of substrate. Substrate was prepared by incubation of 50 mM ribose-5phosphate with 5 U/ml each of ribulose-5-phosphate 3-epimerase and ribose-5-phosphate isomerase for 1 h. The commercially available yeast enzyme (Sigma) was used as a control. One unit is the amo ...
... triosephosphate isomerase, and 40 #1 of substrate. Substrate was prepared by incubation of 50 mM ribose-5phosphate with 5 U/ml each of ribulose-5-phosphate 3-epimerase and ribose-5-phosphate isomerase for 1 h. The commercially available yeast enzyme (Sigma) was used as a control. One unit is the amo ...
amino acid, peptides, proteins, enzymes, and nucleic acids
... figures greatly in the present chapter. We will approach the very important chemistry of amino acids and their derivatives in three stages. First, simple a-amino acids will be considered with emphasis on how the properties of amine functions and of acid functions are modified in molecules that posse ...
... figures greatly in the present chapter. We will approach the very important chemistry of amino acids and their derivatives in three stages. First, simple a-amino acids will be considered with emphasis on how the properties of amine functions and of acid functions are modified in molecules that posse ...
PDF file
... residue 206. Plasmids were sequenced to confirm their authenticity. Expression and Purification of Recombinant Proteins—E. coli BL21(DE3) cells transformed with the GST-PIR1 constructs were grown in LB containing 100 mg/ml ampicilin and 2% (w/v) glucose at 37 °C until the OD600 was 0.7. The cells we ...
... residue 206. Plasmids were sequenced to confirm their authenticity. Expression and Purification of Recombinant Proteins—E. coli BL21(DE3) cells transformed with the GST-PIR1 constructs were grown in LB containing 100 mg/ml ampicilin and 2% (w/v) glucose at 37 °C until the OD600 was 0.7. The cells we ...
Reconstitution of an Allophycocyanin Trimer Complex Containing
... m ain, Lcm polypeptides co n tain tw o to four such repeats, which are also hom ologous to the N -term inal dom ains o f rod and rod-core linkers (B ryant, 1988; H o u m ard e t al., 1990). In phycobil isomes containing Lcm w ith fo u r repeats, the C-term inal dom ain has been suggested to link PC ...
... m ain, Lcm polypeptides co n tain tw o to four such repeats, which are also hom ologous to the N -term inal dom ains o f rod and rod-core linkers (B ryant, 1988; H o u m ard e t al., 1990). In phycobil isomes containing Lcm w ith fo u r repeats, the C-term inal dom ain has been suggested to link PC ...
The Ostrich (Struthio camelus) egg
... Measurement of the apparent affinity constant (Ka, app) for M. luteus cells The initial velocity of lysis was determined at 20°; pH 6.2; I = 0.181 from measurements carried out at 650 nm with a Beckman Acta III spectrophotometer, as already described 8, following the method of LOCQUET et al. 17. It ...
... Measurement of the apparent affinity constant (Ka, app) for M. luteus cells The initial velocity of lysis was determined at 20°; pH 6.2; I = 0.181 from measurements carried out at 650 nm with a Beckman Acta III spectrophotometer, as already described 8, following the method of LOCQUET et al. 17. It ...
Ph.D. Thesis Azar Shahpiri
... acid (GA) signal by synthesizing hydrolytic enzymes that are released to the endosperm, before undergoing cell death. The barley (Hordeum vulgare) aleurone layer can be separated from the other seed tissues and maintained in culture, allowing the study of GA, abscisic acid (ABA) and other signals in ...
... acid (GA) signal by synthesizing hydrolytic enzymes that are released to the endosperm, before undergoing cell death. The barley (Hordeum vulgare) aleurone layer can be separated from the other seed tissues and maintained in culture, allowing the study of GA, abscisic acid (ABA) and other signals in ...
File
... A) are used to form amino acids. B) form between fatty acids. C) are formed by a hydrolysis reaction. D) link amino acids. Answer: D Topic: 3.11 Skill: Knowledge/Comprehension 46) Which of the following statements about enzymes is false? A) They increase the rate of chemical reactions. B) They funct ...
... A) are used to form amino acids. B) form between fatty acids. C) are formed by a hydrolysis reaction. D) link amino acids. Answer: D Topic: 3.11 Skill: Knowledge/Comprehension 46) Which of the following statements about enzymes is false? A) They increase the rate of chemical reactions. B) They funct ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.