Abstract Example
... affecting a number of cellular processes. For instance, Akt has been shown to contribute to cardiac hypertrophy while AMPK is a stress related kinase involved in the control of cardiac energy substrate utilization. Despite these diverse roles, a number of common signaling pathways do exist for these ...
... affecting a number of cellular processes. For instance, Akt has been shown to contribute to cardiac hypertrophy while AMPK is a stress related kinase involved in the control of cardiac energy substrate utilization. Despite these diverse roles, a number of common signaling pathways do exist for these ...
10858_2016_75_MOESM1_ESM
... α-helix region were used with distance cut-off criteria as follows: < 4.5 Å for 1H-1H and 1H-2H pairs and < 2.1 Å for H-15N pairs. These are sequential 1HNi-1HNi+1 distance (2.8 Å), 1HNi-1H Ni+2 distance (4.2 Å), intra residue 1H Ni-2Hαi ...
... α-helix region were used with distance cut-off criteria as follows: < 4.5 Å for 1H-1H and 1H-2H pairs and < 2.1 Å for H-15N pairs. These are sequential 1HNi-1HNi+1 distance (2.8 Å), 1HNi-1H Ni+2 distance (4.2 Å), intra residue 1H Ni-2Hαi ...
Xanthomonas campestris
... in bacterial pathogenicity [30]. Nevertheless, the functions of these genes in bacterial physiology and their relations in metabolism, catabolism, and pathogenicity are not well understood. Most proteins with reduced abundance in this interaction were components of translational machinery (59% ribos ...
... in bacterial pathogenicity [30]. Nevertheless, the functions of these genes in bacterial physiology and their relations in metabolism, catabolism, and pathogenicity are not well understood. Most proteins with reduced abundance in this interaction were components of translational machinery (59% ribos ...
BIOINFORMATICS ORIGINAL PAPER Going from where to why—interpretable prediction of protein subcellular localization
... thus used to draw conclusions about its cellular role, interaction partners and function in biological processes. During the past decade, huge number of novel proteins were discovered in the context of large-scale sequencing projects. Unfortunately, for a majority of these proteins their subcellular ...
... thus used to draw conclusions about its cellular role, interaction partners and function in biological processes. During the past decade, huge number of novel proteins were discovered in the context of large-scale sequencing projects. Unfortunately, for a majority of these proteins their subcellular ...
characteristics and stabilization of dnaase
... were stored separat,ely or recombined at - Is”, some activity was lost compared t’o t,he S-30 fraction (Fig. 2). Ko loss in enzymatic activit#y of fractions S-100 or W-Rib was observed after overnight dialysis. Again, addition of mercapt#o&hanol prevented rapid inactivation. Storage of the fractions ...
... were stored separat,ely or recombined at - Is”, some activity was lost compared t’o t,he S-30 fraction (Fig. 2). Ko loss in enzymatic activit#y of fractions S-100 or W-Rib was observed after overnight dialysis. Again, addition of mercapt#o&hanol prevented rapid inactivation. Storage of the fractions ...
Complement system
... thioester in the C3 to form C3(H2O), allowing binding factor B. factor D plasma protease cleave factor B to form C3(H2O)Bb a fluid C3 convertase, and can cleave C3 to C3a and C3b. Most of these C3b inactivated by H2O ...
... thioester in the C3 to form C3(H2O), allowing binding factor B. factor D plasma protease cleave factor B to form C3(H2O)Bb a fluid C3 convertase, and can cleave C3 to C3a and C3b. Most of these C3b inactivated by H2O ...
Lac
... synthesized in the mammary gland. It is presumed to enter the milk via "leakage" by the paracellular pathway, or by uptake with other components such as immunoglobulins. There does not seem to be a more specific mechanism of transport. Increases in milk concentration of serum albumin occur especiall ...
... synthesized in the mammary gland. It is presumed to enter the milk via "leakage" by the paracellular pathway, or by uptake with other components such as immunoglobulins. There does not seem to be a more specific mechanism of transport. Increases in milk concentration of serum albumin occur especiall ...
Teacher Guide DNA to Protein.ver8 - RI
... • Manipulate the DNA code and predict how it will change the sequence of mRNA and potentially a protein. • Explain the effects of various types of mutations, including substitutions, insertions and deletions, on the resulting transcription and translation of a gene. • Demonstrate how substituting ...
... • Manipulate the DNA code and predict how it will change the sequence of mRNA and potentially a protein. • Explain the effects of various types of mutations, including substitutions, insertions and deletions, on the resulting transcription and translation of a gene. • Demonstrate how substituting ...
Astrovirus Replication: An Overview
... released viruses could partially explain these discrepancies. For several viruses it has been observed that some of their proteins, both structural and nonstructural, undergo caspase-mediated proteolysis within the host cell,29–34 and in some cases such as human influenza virus or Aleutian mink dise ...
... released viruses could partially explain these discrepancies. For several viruses it has been observed that some of their proteins, both structural and nonstructural, undergo caspase-mediated proteolysis within the host cell,29–34 and in some cases such as human influenza virus or Aleutian mink dise ...
Slide 1
... 2) Muscles use fatty acids first, and then augment that with glucose oxidation, thus sparing glucose for periods of high energy output, and sparing bodily glucose for cells that are more directly dependent on it. 3) Fatty acids are mobilized from adipocytes and transported by serum albumin to variou ...
... 2) Muscles use fatty acids first, and then augment that with glucose oxidation, thus sparing glucose for periods of high energy output, and sparing bodily glucose for cells that are more directly dependent on it. 3) Fatty acids are mobilized from adipocytes and transported by serum albumin to variou ...
Teacher Guide DNA to Protein.ver8 - RI
... Manipulate the DNA code and predict how it will change the sequence of mRNA and potentially a protein. Explain the effects of various types of mutations, including substitutions, insertions and deletions, on the resulting transcription and translation of a gene. Demonstrate how substituting on ...
... Manipulate the DNA code and predict how it will change the sequence of mRNA and potentially a protein. Explain the effects of various types of mutations, including substitutions, insertions and deletions, on the resulting transcription and translation of a gene. Demonstrate how substituting on ...
Benchmarking homology detection procedures with low complexity
... techniques such as the Pfam hidden Markov models, common domain assignment is highly sensitive and applicable at far larger evolutionary separation than direct sequence comparison methods such as protein BLAST (Madera and Gough, 2002; Wistrand and Sonnhammer, 2005). If two proteins both have well-de ...
... techniques such as the Pfam hidden Markov models, common domain assignment is highly sensitive and applicable at far larger evolutionary separation than direct sequence comparison methods such as protein BLAST (Madera and Gough, 2002; Wistrand and Sonnhammer, 2005). If two proteins both have well-de ...
Caprotein by Mt. Capra Premium Goat
... With Mt. Capra Caprotein™, you get optimum levels of protein to promote healthy digestion and absorption. This natural supplement is especially valuable to people who are lactose intolerant, thanks to the beneficial properties of goat milk. Here are some important facts about the differences betwee ...
... With Mt. Capra Caprotein™, you get optimum levels of protein to promote healthy digestion and absorption. This natural supplement is especially valuable to people who are lactose intolerant, thanks to the beneficial properties of goat milk. Here are some important facts about the differences betwee ...
Valine Mydrogenase from Streptmzyces fiadipe
... The oxidativedeaminationactivityof VDH was measured as the increase of NADH absorbanceat 340 nm. The reaction mixture (1 ml) contained: 100 pmol glycine/KCl/KOH buffer, 10 pmol L-valine, 1.5 pmol NAD+ and 10 pmol2-mercaptoethanol;the final pH was 10-6,temperature 30 "C.One enzyme activity unit was d ...
... The oxidativedeaminationactivityof VDH was measured as the increase of NADH absorbanceat 340 nm. The reaction mixture (1 ml) contained: 100 pmol glycine/KCl/KOH buffer, 10 pmol L-valine, 1.5 pmol NAD+ and 10 pmol2-mercaptoethanol;the final pH was 10-6,temperature 30 "C.One enzyme activity unit was d ...
Sterilization & Disinfection
... The killing of microbes by either chemicals or radiation is proportional to the dose, which is defined as the product of the concentration multiplied by the time of exposure. ...
... The killing of microbes by either chemicals or radiation is proportional to the dose, which is defined as the product of the concentration multiplied by the time of exposure. ...
In vitro gastrointestinal digestion study of a novel bio-tofu
... residues of protein bound glutamine and lysine (Hsieh et al., 2014). Soy proteins and caseins are known to be good substrates for MTGase. Enzymatic modification of proteins by MTGase provides protein to distribute more homogeneously and evenly in network which increases the gel stability and in turn ...
... residues of protein bound glutamine and lysine (Hsieh et al., 2014). Soy proteins and caseins are known to be good substrates for MTGase. Enzymatic modification of proteins by MTGase provides protein to distribute more homogeneously and evenly in network which increases the gel stability and in turn ...
Lecture Note 1
... applications in life sciences and medicine. Liquid chromatography turned out to be a major advancement towards achieving sensitivity and power of resolving the closelyrelated metabolites. Reversed-phase chromatography, for example, has proved to be an excellent tool for resolving and analyzing the s ...
... applications in life sciences and medicine. Liquid chromatography turned out to be a major advancement towards achieving sensitivity and power of resolving the closelyrelated metabolites. Reversed-phase chromatography, for example, has proved to be an excellent tool for resolving and analyzing the s ...
A typical hemoglobin molecule consists of a "heme" part and a
... A typical hemoglobin molecule consists of a "heme" part and a protein part=globin. The "heme" part lies in the interior of the hemoglobin molecule. At the center of the heme is an Fe(II), ferrous, atom. Four of the six coordination sites around this atom are occupied by nitrogen atoms from a porphyr ...
... A typical hemoglobin molecule consists of a "heme" part and a protein part=globin. The "heme" part lies in the interior of the hemoglobin molecule. At the center of the heme is an Fe(II), ferrous, atom. Four of the six coordination sites around this atom are occupied by nitrogen atoms from a porphyr ...
Biol 1107 Biomolecules Lab Fall 2003
... agarose gel also contains ethidium bromide which binds to the DNA or RNA and fluoresces under uv light. Thus, after the nucleic acids have been separated by electrophoresis, the gel is placed under uv light to visualize the DNA or RNA. The locations of the DNA or RNA appear as bands within the gel, ...
... agarose gel also contains ethidium bromide which binds to the DNA or RNA and fluoresces under uv light. Thus, after the nucleic acids have been separated by electrophoresis, the gel is placed under uv light to visualize the DNA or RNA. The locations of the DNA or RNA appear as bands within the gel, ...
Monoclonal antibodies - Federation of American Societies for
... the blood had an infinite ability to combine with any substance, yet superficially seemed to be a nearly uniform group of proteins. This confounded him and bolstered his determination to decipher antibody structure and function. In the late 1950’s, he took an innovative approach to the problem. Inst ...
... the blood had an infinite ability to combine with any substance, yet superficially seemed to be a nearly uniform group of proteins. This confounded him and bolstered his determination to decipher antibody structure and function. In the late 1950’s, he took an innovative approach to the problem. Inst ...
Increased MS Protein Identifi cation Rates Using 75 cm Long nano
... Peptide and Protein Identifications From a proteomics perspective, researchers in the field are commonly interested in the number of peptides identified, either in terms of peptide spectral matches, unique peptides or protein groups. As shown in Figure 4, the 75 cm column consistently results in the ...
... Peptide and Protein Identifications From a proteomics perspective, researchers in the field are commonly interested in the number of peptides identified, either in terms of peptide spectral matches, unique peptides or protein groups. As shown in Figure 4, the 75 cm column consistently results in the ...
COT102 Decision Document draft only
... The cyclopropenoid fatty acids (CPFA) including sterculic, malvalic, and dihydrosterculic are unique fatty acids in cotton that are considered undesirable, anti-nutrients (OECD, 2008) Regulatory Decision After reviewing the scientific data and information relevant to the application of Syngenta Phil ...
... The cyclopropenoid fatty acids (CPFA) including sterculic, malvalic, and dihydrosterculic are unique fatty acids in cotton that are considered undesirable, anti-nutrients (OECD, 2008) Regulatory Decision After reviewing the scientific data and information relevant to the application of Syngenta Phil ...
Show DNA to Protein HC
... • Split genes can code for different proteins or different regions of same polypeptide • Introns increase the cross over frequency between 2 alleles which increases diversity ...
... • Split genes can code for different proteins or different regions of same polypeptide • Introns increase the cross over frequency between 2 alleles which increases diversity ...
Citrátový cyklus a dýchací řetězec
... (e- and H+) in electron transport system. •location: inner mitochondrial membrane •composition: enzyme complexes I – IV, 2 mobile carriers of electrons – coenzyme Q (ubiquinone) and cytochrome c •function: transport of electrons in series of redox reactions and H+. Oxygen is a final acceptor of elec ...
... (e- and H+) in electron transport system. •location: inner mitochondrial membrane •composition: enzyme complexes I – IV, 2 mobile carriers of electrons – coenzyme Q (ubiquinone) and cytochrome c •function: transport of electrons in series of redox reactions and H+. Oxygen is a final acceptor of elec ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.