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... Supplementary Fig. 1. Homology model of RT-460 generated by using 1V04 as a template. Panel A shows a ribbon diagram representing the modelled structure of RT-460 enzyme, viewed along the axis with the catalytic and the structural calcium (yellow spheres). Mutated amino acid residues (H115W, R192K, ...
... Supplementary Fig. 1. Homology model of RT-460 generated by using 1V04 as a template. Panel A shows a ribbon diagram representing the modelled structure of RT-460 enzyme, viewed along the axis with the catalytic and the structural calcium (yellow spheres). Mutated amino acid residues (H115W, R192K, ...
Human Signaling Protein 14-3-3 Interacts With
... Yeast two-hybrid constructs and transformation. The cytoplasmic domains of platelet GPIba and GPIbb were each polymerase chain reaction (PCR)-amplified from platelet cDNA generated by platelet RNA reverse transcription. Platelet RNA was isolated from the therapeutic pheresis material of a patient wi ...
... Yeast two-hybrid constructs and transformation. The cytoplasmic domains of platelet GPIba and GPIbb were each polymerase chain reaction (PCR)-amplified from platelet cDNA generated by platelet RNA reverse transcription. Platelet RNA was isolated from the therapeutic pheresis material of a patient wi ...
Protein conformational changes induced by adsorption onto material
... and plasminogen [40]. This has been nicely shown by Brash and co-workers, using radioactively labeled proteins to follow their binding on glass [41]. Since competition depends on the dissociation rate constant of the protein from the surface, it is of importance to characterize this parameter. Many ...
... and plasminogen [40]. This has been nicely shown by Brash and co-workers, using radioactively labeled proteins to follow their binding on glass [41]. Since competition depends on the dissociation rate constant of the protein from the surface, it is of importance to characterize this parameter. Many ...
Sato Talk
... while the origin of mitochondria is likely to be an ancestor of alpha proteobacteria. This inference is mainly supported by the comparison of genomic sequences of the organelles and various bacteria. However, we recently pointed out that, based on accumulating database information and analytical dat ...
... while the origin of mitochondria is likely to be an ancestor of alpha proteobacteria. This inference is mainly supported by the comparison of genomic sequences of the organelles and various bacteria. However, we recently pointed out that, based on accumulating database information and analytical dat ...
Supplementary materials
... protein gels. Left panel: 2-D patterns of the soluble-fraction proteins isolated from S. oneidensis MR-1 after whole-cell lysate. Proteins were separated by two-dimensional gel electrophoresis with isoeletric focusing in the first dimension and sodium dodecyl sulfate electrophoresis in the second di ...
... protein gels. Left panel: 2-D patterns of the soluble-fraction proteins isolated from S. oneidensis MR-1 after whole-cell lysate. Proteins were separated by two-dimensional gel electrophoresis with isoeletric focusing in the first dimension and sodium dodecyl sulfate electrophoresis in the second di ...
Module 17 / Microscopic Level—The Sarcomere
... Histological sections of muscle show the anatomical features of the sarcomeres. Thick filaments, composed of myosin, are visible as the A band of a sarcomere. Thin filaments, composed of actin, attach to a protein in the Z disc (or Z line) called alpha-actinin, and they are present across the entire ...
... Histological sections of muscle show the anatomical features of the sarcomeres. Thick filaments, composed of myosin, are visible as the A band of a sarcomere. Thin filaments, composed of actin, attach to a protein in the Z disc (or Z line) called alpha-actinin, and they are present across the entire ...
Option 3 Forensic chemistry
... explain how it operates. Explain how it can be used in forensic analysis. 34. Use secondary sources to analyse examples of how analytical techniques can be used by forensic chemists. Revision Questions - Jacaranda Chemistry 2. Set ...
... explain how it operates. Explain how it can be used in forensic analysis. 34. Use secondary sources to analyse examples of how analytical techniques can be used by forensic chemists. Revision Questions - Jacaranda Chemistry 2. Set ...
Characterizing transcription factor binding sites using formaldehyde
... be optimized for binding of the factor that actually contacts DNA. An example of a binding site that is refractory to in vitro analysis comes from the ChET8 promoter. ChET8 was originally identified in a screen for E2F1 target genes and was confirmed as containing a strong in vivo binding site for mul ...
... be optimized for binding of the factor that actually contacts DNA. An example of a binding site that is refractory to in vitro analysis comes from the ChET8 promoter. ChET8 was originally identified in a screen for E2F1 target genes and was confirmed as containing a strong in vivo binding site for mul ...
Saito et al, 2009 - The EMBO Journal
... the membrane in a GTP-bound form and nucleotide hydrolysis parallels events leading to separation of clathrin-coated vesicles from the plasma membrane (Doherty and McMahon, 2009). Surprisingly, the formation of clathrin-coated vesicles at the plasma membrane in Saccharomyces cerevisiae and at the TG ...
... the membrane in a GTP-bound form and nucleotide hydrolysis parallels events leading to separation of clathrin-coated vesicles from the plasma membrane (Doherty and McMahon, 2009). Surprisingly, the formation of clathrin-coated vesicles at the plasma membrane in Saccharomyces cerevisiae and at the TG ...
Physical Chemistry, Short talk PC-013 Protein
... In this contribution we present strategies to characterize protein-nucleotide interactions in large biological assemblies using the example of the (double-) hexameric DnaB helicase from Helicobacter pylori with a molecular weight of 672 kDa. The binding of nucleotides (ATP-analogues and single-stran ...
... In this contribution we present strategies to characterize protein-nucleotide interactions in large biological assemblies using the example of the (double-) hexameric DnaB helicase from Helicobacter pylori with a molecular weight of 672 kDa. The binding of nucleotides (ATP-analogues and single-stran ...
Fluorescence Spectroscopy
... incandescent lamp or a laser and absorbed by the fluorophore, creating an excited electronic singlet state (S1'). This process distinguishes fluorescence from chemiluminescence, in which the excited state is populated by a chemical reaction. Stage 2 : Excited-State Lifetime. The excited state exists ...
... incandescent lamp or a laser and absorbed by the fluorophore, creating an excited electronic singlet state (S1'). This process distinguishes fluorescence from chemiluminescence, in which the excited state is populated by a chemical reaction. Stage 2 : Excited-State Lifetime. The excited state exists ...
Creating Multiple Sequence Alignments
... change ‘Model’ to Amino Acid|No. of differences. (leave the default parameters in the other options). Click on Compute. E2. See the tree in the Tree Explorer window. E3. To select a branch, left-click on it. If you right-click on a branch, you will find several options to perform different operation ...
... change ‘Model’ to Amino Acid|No. of differences. (leave the default parameters in the other options). Click on Compute. E2. See the tree in the Tree Explorer window. E3. To select a branch, left-click on it. If you right-click on a branch, you will find several options to perform different operation ...
Homology Modeling Zinc Fingers – Introduction zf
... the residues that are used in DNA recognition. The Consensus Server, developed in part by Dr. Camacho, was used to perform the homology modeling (http://structure.bu.edu/cgi-bin/consensus/consensus.cgi) Since threading algorithms are used in the Consensus method, the side chains of amino acids can o ...
... the residues that are used in DNA recognition. The Consensus Server, developed in part by Dr. Camacho, was used to perform the homology modeling (http://structure.bu.edu/cgi-bin/consensus/consensus.cgi) Since threading algorithms are used in the Consensus method, the side chains of amino acids can o ...
02 NCAC 09E .0109 NON‑PROTEIN NITROGEN (a) Urea and other
... The directions for use and the caution statement shall be in type of such size so placed on the label that they will be read and understood by ordinary persons under customary conditions of purchase and use. (c) The labeling of all feeds containing non-protein nitrogen ingredients, additional to oth ...
... The directions for use and the caution statement shall be in type of such size so placed on the label that they will be read and understood by ordinary persons under customary conditions of purchase and use. (c) The labeling of all feeds containing non-protein nitrogen ingredients, additional to oth ...
FEED NUTRIENTS
... Proteins are complex organic macromolecules containing carbon, hydrogen, oxygen, nitrogen, and usually sulfur. They consist of one or more chains of amino acids. Proteins are fundamental components of all body cells and include many substances (such as enzymes, hormones, and antibodies) necessary fo ...
... Proteins are complex organic macromolecules containing carbon, hydrogen, oxygen, nitrogen, and usually sulfur. They consist of one or more chains of amino acids. Proteins are fundamental components of all body cells and include many substances (such as enzymes, hormones, and antibodies) necessary fo ...
Mapping Enzyme Active Sites in Complex Proteomes
... of mechanistic studies of particular enzyme classes to create chemical probes with predictable proteome reactivities. By incorporating as the RG well-known affinity labels, ABPP probes have been developed that target, for example, serine hydrolases5,7 and cysteine proteases.8 In each of these cases, ...
... of mechanistic studies of particular enzyme classes to create chemical probes with predictable proteome reactivities. By incorporating as the RG well-known affinity labels, ABPP probes have been developed that target, for example, serine hydrolases5,7 and cysteine proteases.8 In each of these cases, ...
Electromagnetic Properties of Biomolecules
... range includes IR, visible and UV light. These computational predictions were confirmed by comparison of: a) Absorption characteristics of light absorbing proteins and their characteristic frequencies, b) Frequency selective light effects on cell growth and characteristic frequencies of growth facto ...
... range includes IR, visible and UV light. These computational predictions were confirmed by comparison of: a) Absorption characteristics of light absorbing proteins and their characteristic frequencies, b) Frequency selective light effects on cell growth and characteristic frequencies of growth facto ...
EF-Tu PROTEIN DOMAINS
... as it plays a key role in protein biosynthesis. It is a model, multifunctional GTP-protein. This protein is also in the centre of interest as a possible target for new antibiotics. Moreover, the high homology in structure and function makes EF-Tu proteins suitable for the studies of evolutionary rel ...
... as it plays a key role in protein biosynthesis. It is a model, multifunctional GTP-protein. This protein is also in the centre of interest as a possible target for new antibiotics. Moreover, the high homology in structure and function makes EF-Tu proteins suitable for the studies of evolutionary rel ...
The Chemistry of Burgers
... The burger part of a cheeseburger is short for hamburger, which is what Americans call a cooked patty of ground meat, usually beef. Immigrants to the U.S. in the early 1900s brought with them favorite recipes, one of which was shredded or minced cooked beef, a back-home favorite of many Germans, som ...
... The burger part of a cheeseburger is short for hamburger, which is what Americans call a cooked patty of ground meat, usually beef. Immigrants to the U.S. in the early 1900s brought with them favorite recipes, one of which was shredded or minced cooked beef, a back-home favorite of many Germans, som ...
A single amino acid change, Q114R, in the cleavage
... cleavage-site mutants exhibited a marked decrease in their replication rate compared with rNDV. The mutant rNDVQ114R, K115R had 1.0 log10 lower and rNDV-Q114R, I118V, rNDV-Q114R, K115R, I118V and rNDV-Q114R had 1.5–2.0 log10 lower virus yield compared with rNDV at 48 h p.i. This result corroborated ...
... cleavage-site mutants exhibited a marked decrease in their replication rate compared with rNDV. The mutant rNDVQ114R, K115R had 1.0 log10 lower and rNDV-Q114R, I118V, rNDV-Q114R, K115R, I118V and rNDV-Q114R had 1.5–2.0 log10 lower virus yield compared with rNDV at 48 h p.i. This result corroborated ...
Topic 3 The chemistry of life
... the reactions may occur faster. Enzymes are organic catalysts. They are proteins. The amino acids that make up these enzymes allow a tertiary and/or quaternary structure. Because each enzyme has a specific amino acid sequence, enzymes have a specific three-dimensional shape. The molecule an enzy ...
... the reactions may occur faster. Enzymes are organic catalysts. They are proteins. The amino acids that make up these enzymes allow a tertiary and/or quaternary structure. Because each enzyme has a specific amino acid sequence, enzymes have a specific three-dimensional shape. The molecule an enzy ...
Topic 3 The chemistry of life
... the reactions may occur faster. Enzymes are organic catalysts. They are proteins. The amino acids that make up these enzymes allow a tertiary and/or quaternary structure. Because each enzyme has a specific amino acid sequence, enzymes have a specific three-dimensional shape. The molecule an enzy ...
... the reactions may occur faster. Enzymes are organic catalysts. They are proteins. The amino acids that make up these enzymes allow a tertiary and/or quaternary structure. Because each enzyme has a specific amino acid sequence, enzymes have a specific three-dimensional shape. The molecule an enzy ...
TheraGest - ProThera
... down fat, carbohydrate, and protein. Digestive enzymes assist the body in breaking down large food particles into smaller particles that can be efficiently absorbed by the intestinal tract. Improper digestion can lead to abdominal discomfort such as bloating, feeling of fullness and constipation as ...
... down fat, carbohydrate, and protein. Digestive enzymes assist the body in breaking down large food particles into smaller particles that can be efficiently absorbed by the intestinal tract. Improper digestion can lead to abdominal discomfort such as bloating, feeling of fullness and constipation as ...
Waiting for the sun to come out: How photosynthesis adapts to
... regulates biomass accumulation depending on levels of ambient light. Tic62 itself forms part of the molecular complex that enables specific proteins to be imported into the chloroplast from the surrounding cytoplasm, and was already known to anchor FNR to the so-called thylakoids, the photosynthetic ...
... regulates biomass accumulation depending on levels of ambient light. Tic62 itself forms part of the molecular complex that enables specific proteins to be imported into the chloroplast from the surrounding cytoplasm, and was already known to anchor FNR to the so-called thylakoids, the photosynthetic ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.