BLAST
... all, the signal-to-noise ratio is greatly improved for the specific purpose of identifying protein relatives. It is accepted that convergence phenomena in aa sequences are very rare and thus aa similarity almost always means homology. Furthermore, aa sequences may still show a similarity derived fro ...
... all, the signal-to-noise ratio is greatly improved for the specific purpose of identifying protein relatives. It is accepted that convergence phenomena in aa sequences are very rare and thus aa similarity almost always means homology. Furthermore, aa sequences may still show a similarity derived fro ...
KASH `n Karry: The KASH domain family of cargo
... in one-cell embryos and also for many subsequent cell cycles. zyg-12 mutants die as embryos due to cellular aneuploidy. In addition, ZYG-12 protein isoforms are small. There are three ZYG-12 isoforms (Fig. 4), two that contain KASH domains (B and C) and one that does not (A). Also, N-terminal to its ...
... in one-cell embryos and also for many subsequent cell cycles. zyg-12 mutants die as embryos due to cellular aneuploidy. In addition, ZYG-12 protein isoforms are small. There are three ZYG-12 isoforms (Fig. 4), two that contain KASH domains (B and C) and one that does not (A). Also, N-terminal to its ...
Gene Section MAP2 (microtubule associated protein 2) -
... long repeating motif is characteristic of this protein. However, it is found that this motif is not sufficient by itself to bind to microtubules. Two contiguous sequences on either end of this repeating structure on both the amino and carboxyl ends enable the binding of this protein to the microtubu ...
... long repeating motif is characteristic of this protein. However, it is found that this motif is not sufficient by itself to bind to microtubules. Two contiguous sequences on either end of this repeating structure on both the amino and carboxyl ends enable the binding of this protein to the microtubu ...
Document
... so as to yield a straight line for reactions obeying Michaelis-Menten kinetics. This is done by plotting reaction velocity (V) versus velocity/substrate concentration (V/[S]). The slope of the line is equal to -KM and the x-intercept is Vmax ...
... so as to yield a straight line for reactions obeying Michaelis-Menten kinetics. This is done by plotting reaction velocity (V) versus velocity/substrate concentration (V/[S]). The slope of the line is equal to -KM and the x-intercept is Vmax ...
pGLO Transformation and Green Fluorescent Protein - Bio-Rad
... • Medium that contains nutrients for bacterial growth and gene expression – Carbohydrates – Amino acids – Nucleotides – Salts – Vitamins ...
... • Medium that contains nutrients for bacterial growth and gene expression – Carbohydrates – Amino acids – Nucleotides – Salts – Vitamins ...
pGLO Transformation and Purification of Green
... • Medium that contains nutrients for bacterial growth and gene expression – Carbohydrates – Amino acids – Nucleotides – Salts – Vitamins ...
... • Medium that contains nutrients for bacterial growth and gene expression – Carbohydrates – Amino acids – Nucleotides – Salts – Vitamins ...
Effects of Anions on the Positive Ion Electrospray Ionization Mass
... phase (by positive ion electrospray ionization mass spectrometry) with the net charge residing on the protein in solution will require that the described anion effect be taken into account. In addition, it appears that some control over the distribution of charge states on peptides and protein ions ...
... phase (by positive ion electrospray ionization mass spectrometry) with the net charge residing on the protein in solution will require that the described anion effect be taken into account. In addition, it appears that some control over the distribution of charge states on peptides and protein ions ...
Fish Protein Hydrolysate Production by Acid and Enzymatic Hydrolysis
... conditions and increases utilization of fish protein hydrolysis due to the better nutritional properties of the final product [6]. Even though enzymatic hydrolysis needs longer reaction time and requires higher production cost, the fish protein hydrolysate obtained would be more marketable and valua ...
... conditions and increases utilization of fish protein hydrolysis due to the better nutritional properties of the final product [6]. Even though enzymatic hydrolysis needs longer reaction time and requires higher production cost, the fish protein hydrolysate obtained would be more marketable and valua ...
This Article Abstract Full Text (PDF) Alert me when this article is cited
... h on LB-kanamycin (or LB-ampicillin) containing 0.02% L-arabinose. The colonies were scraped into phosphate-buffered saline, and viable counts were determined from this suspension. Cells were harvested by centrifugation, leaving cell-free polysaccharide in the supernatant. The supernatant was treate ...
... h on LB-kanamycin (or LB-ampicillin) containing 0.02% L-arabinose. The colonies were scraped into phosphate-buffered saline, and viable counts were determined from this suspension. Cells were harvested by centrifugation, leaving cell-free polysaccharide in the supernatant. The supernatant was treate ...
Metabolism of “surplus” amino acids
... For an adult in N balance, apart from small amounts of amino acids required for the synthesis of neurotransmitters, hormones, etc, an amount of amino acids almost equal to that absorbed from the diet can be considered to be “surplus” in that it will be catabolized. The higher diet-induced thermogene ...
... For an adult in N balance, apart from small amounts of amino acids required for the synthesis of neurotransmitters, hormones, etc, an amount of amino acids almost equal to that absorbed from the diet can be considered to be “surplus” in that it will be catabolized. The higher diet-induced thermogene ...
IOSR Journal of Applied Chemistry (IOSR-JAC) e-ISSN: 2278-5736.
... The docking studies were performed on HVR protein (HIV-1 protease) and from the studies it was found that the compound SR-03[Resorcinol derivative (dock score -99.07 and hydrogen bond energy -7.17 KJ)] was found to have the greatest affinity to HVR protein (HIV-1 protease) followed by SR-02[Benzyl d ...
... The docking studies were performed on HVR protein (HIV-1 protease) and from the studies it was found that the compound SR-03[Resorcinol derivative (dock score -99.07 and hydrogen bond energy -7.17 KJ)] was found to have the greatest affinity to HVR protein (HIV-1 protease) followed by SR-02[Benzyl d ...
Isolation and characterization of cDNA clones encoding
... Induction of PR-proteins in TMV infected tobacco plants Equal amounts of protein isolated from TMV infected and mock inoculated tobacco plants (Nicoiiana labacum cv. Samsun NN) were separated on SDS-polyacrylamide gels and blotted onto nitrocellulose membranes. When these filters were probed with a ...
... Induction of PR-proteins in TMV infected tobacco plants Equal amounts of protein isolated from TMV infected and mock inoculated tobacco plants (Nicoiiana labacum cv. Samsun NN) were separated on SDS-polyacrylamide gels and blotted onto nitrocellulose membranes. When these filters were probed with a ...
Managing people in sport organisations
... Copyright © 2009 by Academic Press. All rights reserved. ...
... Copyright © 2009 by Academic Press. All rights reserved. ...
Probing the conformational changes of the yeast mitochondrial ADP
... very grateful to all Kunji lab members past and present. Dr Christof Bös, Dr Alex Hellawell and Dr John Mifsud provided essential training. Liz Cerson always had time to listen and be supportive through any 'life crisis'. ...
... very grateful to all Kunji lab members past and present. Dr Christof Bös, Dr Alex Hellawell and Dr John Mifsud provided essential training. Liz Cerson always had time to listen and be supportive through any 'life crisis'. ...
Myoglobin from equine skeletal muscle (M0630)
... CAS Number: 100684-32-0 Molecular Weight: 17.6 kDa1 Extinction Coefficient: EmM = 12.92 (555 nm)2 pI: 7.3 (major component) and 6.8 (minor component)3 Myoglobin from horse skeletal muscle is a single chain heme protein containing 153 amino acid residues. It posesses no disulfide bridges or free -SH ...
... CAS Number: 100684-32-0 Molecular Weight: 17.6 kDa1 Extinction Coefficient: EmM = 12.92 (555 nm)2 pI: 7.3 (major component) and 6.8 (minor component)3 Myoglobin from horse skeletal muscle is a single chain heme protein containing 153 amino acid residues. It posesses no disulfide bridges or free -SH ...
Proteases and proteolytic cleavage of storage
... known (Thanh and Shibasaki, 1977), the latter composed of different ratios of large and small subunits (Mtintz et al, 1986). Amino acid sequences of the large and small subunits are very homologous. Close to their N-terminus the large subunits contain an approximately 20 kDa large, strongly hydrophi ...
... known (Thanh and Shibasaki, 1977), the latter composed of different ratios of large and small subunits (Mtintz et al, 1986). Amino acid sequences of the large and small subunits are very homologous. Close to their N-terminus the large subunits contain an approximately 20 kDa large, strongly hydrophi ...
Dual-topology membrane proteins Escherichia coli Susanna Seppälä
... bacterium. It is part of the normal gut flora in humans and other animals, although there are pathogenic variants that may cause disease. Since its discovery by Theodor Escherich in 1885, E. coli has become a popular workhorse in laboratories worldwide, the main reasons being that the cells are easy ...
... bacterium. It is part of the normal gut flora in humans and other animals, although there are pathogenic variants that may cause disease. Since its discovery by Theodor Escherich in 1885, E. coli has become a popular workhorse in laboratories worldwide, the main reasons being that the cells are easy ...
ATP
... water to drink she would be dead within a week. Hoping to postpone the inevitable, her thirst drove her to drink the salty seawater. She was dead in two days. Why did drinking seawater kill the sailor faster than not drinking any water at all? ...
... water to drink she would be dead within a week. Hoping to postpone the inevitable, her thirst drove her to drink the salty seawater. She was dead in two days. Why did drinking seawater kill the sailor faster than not drinking any water at all? ...
Support Vector Machine-based classification of protein folds using
... become a necessary alternative to experimental determination of 3D structures. Among the computational approaches, fold recognition/threading methods have taken central stage. In instances where detection of homology becomes difficult even when using the best sequence comparison methods such as PSI- ...
... become a necessary alternative to experimental determination of 3D structures. Among the computational approaches, fold recognition/threading methods have taken central stage. In instances where detection of homology becomes difficult even when using the best sequence comparison methods such as PSI- ...
Lecture3
... Biosynthesis of protein comprises a series of processes involving DNA, which carries the genetic information, determines the exact structure of the protein to be synthesized. DNA controls the formation of the 3 types of RNA which determine the sequence of the amino acid and the molecular size of the ...
... Biosynthesis of protein comprises a series of processes involving DNA, which carries the genetic information, determines the exact structure of the protein to be synthesized. DNA controls the formation of the 3 types of RNA which determine the sequence of the amino acid and the molecular size of the ...
MB ChB PHASE I
... N FLOW THROUGH THE BIOSPHERE N in biomolecules comes ultimately from N2 (80% of the atmosphere). However, N2 isn’t readily exchanged with N found in organisms. ...
... N FLOW THROUGH THE BIOSPHERE N in biomolecules comes ultimately from N2 (80% of the atmosphere). However, N2 isn’t readily exchanged with N found in organisms. ...
gene cloning and identification of the Circumsporozoite protein of
... Chemicals, Piscataway, N.J.). Antibodies were radiolabeled with 125I by using lodogen (Pierce Chemical Co., Rockford, Ill.) according to the instructions of the manufacturers. Immunoassays. A two-site immunoradiometric assay (IRMA) was performed as described in reference 34. Flexible microtiter plat ...
... Chemicals, Piscataway, N.J.). Antibodies were radiolabeled with 125I by using lodogen (Pierce Chemical Co., Rockford, Ill.) according to the instructions of the manufacturers. Immunoassays. A two-site immunoradiometric assay (IRMA) was performed as described in reference 34. Flexible microtiter plat ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.