Ideas on Cell Respiration Demos
... fashion a 3-dimensional model of meiosis which was movable through the phases. They came up with excellent clever models that I wouldn't have thought of myself. They used snaps, velcro, felt, shoeboxes, aluminum pie plates, beads, and many other props. They had to present and explain each model to t ...
... fashion a 3-dimensional model of meiosis which was movable through the phases. They came up with excellent clever models that I wouldn't have thought of myself. They used snaps, velcro, felt, shoeboxes, aluminum pie plates, beads, and many other props. They had to present and explain each model to t ...
Selection of Functional Signal Peptide Cleavage Sites from a Library of Random Sequences.
... Selection of active mutants. To select for functional mutants, plasmid DNA from the random-sequence library was used to transform E. coli TG1 by the CaCl2 method (21). The transformation mixture was plated on Luria-Bertani plates containing 1 mg of ampicillin, 500 ,ug of ampicillin, or 12.5 ,ug of c ...
... Selection of active mutants. To select for functional mutants, plasmid DNA from the random-sequence library was used to transform E. coli TG1 by the CaCl2 method (21). The transformation mixture was plated on Luria-Bertani plates containing 1 mg of ampicillin, 500 ,ug of ampicillin, or 12.5 ,ug of c ...
Function and biotechnology of extremophilic enzymes in low water
... diminishing the number of intermolecular hydrogen bonds [20-22]. High salt concentrations critically affect the solubility, binding, stability, and crystallization of proteins [23]. The interactions between proteins and protein subunits in solution are also altered by salts. The electrostatic intera ...
... diminishing the number of intermolecular hydrogen bonds [20-22]. High salt concentrations critically affect the solubility, binding, stability, and crystallization of proteins [23]. The interactions between proteins and protein subunits in solution are also altered by salts. The electrostatic intera ...
LowSlides
... INTRACELLULAR RECEPTORS:“LIGAND-ACTIVATED TRANSCRIPTION FACTORS” HORMONES WITH INTRACELLULAR RECEPTORS ARE HYDROPHOBIC ALLOWING ...
... INTRACELLULAR RECEPTORS:“LIGAND-ACTIVATED TRANSCRIPTION FACTORS” HORMONES WITH INTRACELLULAR RECEPTORS ARE HYDROPHOBIC ALLOWING ...
Stability, catalytic versatility and evolution of the
... [1]. In their natural environment, enzymes need to be stable in order to maintain their native structures but also have to be flexible to allow conformational changes during catalysis. These opposing requirements are particularly striking for enzymes from extremophiles, which must be both stable and ...
... [1]. In their natural environment, enzymes need to be stable in order to maintain their native structures but also have to be flexible to allow conformational changes during catalysis. These opposing requirements are particularly striking for enzymes from extremophiles, which must be both stable and ...
Organic Compounds Essential to Human Functioning
... some macromolecules are made up of several copies of single units called monomer (mono- = one; -mer = part). Like beads in a long necklace, these monomers link by covalent bonds to form long polymers (poly= many). There are many examples of monomers and polymers among the organic compounds. ...
... some macromolecules are made up of several copies of single units called monomer (mono- = one; -mer = part). Like beads in a long necklace, these monomers link by covalent bonds to form long polymers (poly= many). There are many examples of monomers and polymers among the organic compounds. ...
Structural Characterization of Humanized Nanobodies with
... structure-stabilizing bridge in a β-roll structure within each RTX-Block region [10–12]. Moreover, CyaA is synthesized as an inactive precursor which requires a palmitoyl group be added at Lys983 by CyaC acyltransferase [7,13,14]. The CyaA-RTX subdomain is involved in toxin binding to target cells t ...
... structure-stabilizing bridge in a β-roll structure within each RTX-Block region [10–12]. Moreover, CyaA is synthesized as an inactive precursor which requires a palmitoyl group be added at Lys983 by CyaC acyltransferase [7,13,14]. The CyaA-RTX subdomain is involved in toxin binding to target cells t ...
Amicon® Ultra-15 Centrifugal Filter Devices
... the provided centrifuge tube. The device can be spun in a swinging-bucket or fixed-angle rotor. Amicon® Ultra-15 devices are supplied non-sterile and are for single use only. The Amicon® Ultra-15 product line includes 5 different cutoffs (MWCO): ●● Amicon® Ultra 3K device — 3,000 MWCO ●● Amicon® Ult ...
... the provided centrifuge tube. The device can be spun in a swinging-bucket or fixed-angle rotor. Amicon® Ultra-15 devices are supplied non-sterile and are for single use only. The Amicon® Ultra-15 product line includes 5 different cutoffs (MWCO): ●● Amicon® Ultra 3K device — 3,000 MWCO ●● Amicon® Ult ...
Nutrition For Runners
... Not consuming enough calories after workouts Swayed by the “magic bullet” ...
... Not consuming enough calories after workouts Swayed by the “magic bullet” ...
Hitting the Target: Emerging Technologies in the Search for Kinase
... munoblotting of the majority of the proteins recognized by this antibody. Our laboratory used the apparent molecular size of protein bands on phospho-Akt substrate immunoblots to narrow down searches for candidate Akt substrates with Scansite (16). This approach successfully identified known and nov ...
... munoblotting of the majority of the proteins recognized by this antibody. Our laboratory used the apparent molecular size of protein bands on phospho-Akt substrate immunoblots to narrow down searches for candidate Akt substrates with Scansite (16). This approach successfully identified known and nov ...
Unit 3
... 2. To name the four “pendant bases” and describe the interactions between polymer chains that lead to the double helix. 3. To describe the process of replication of DNA and how complimentary bases end up producing a reliable copy of the original. 4. To describe how a three-base sequence, called a co ...
... 2. To name the four “pendant bases” and describe the interactions between polymer chains that lead to the double helix. 3. To describe the process of replication of DNA and how complimentary bases end up producing a reliable copy of the original. 4. To describe how a three-base sequence, called a co ...
4-6
... A major stimulus of muscle protein catabolism in ARF is insulin resistance. In muscle, the maximal rate of insulin-stimulated protein synthesis is depressed by ARF and protein degradation is increased even in the presence of insulin [9]. Acidosis was identified as an important factor in muscle prote ...
... A major stimulus of muscle protein catabolism in ARF is insulin resistance. In muscle, the maximal rate of insulin-stimulated protein synthesis is depressed by ARF and protein degradation is increased even in the presence of insulin [9]. Acidosis was identified as an important factor in muscle prote ...
Proteomic profiling of non-obese type 2 diabetic skeletal muscle
... Comparative gel electrophoretic analysis of normal versus diabetic skeletal muscle. In order to determine potential differences in the skeletal muscle proteome from normal Wistar rats versus diabetic GK rats, crude total tissue extracts were separated by high-resolution two-dimensional gel electroph ...
... Comparative gel electrophoretic analysis of normal versus diabetic skeletal muscle. In order to determine potential differences in the skeletal muscle proteome from normal Wistar rats versus diabetic GK rats, crude total tissue extracts were separated by high-resolution two-dimensional gel electroph ...
Biology Unit 1-Quiz #2A
... ________ 37) Which of the following is accurate? A- When an enzyme catalyzes a reaction, the substrate from the reaction must bind to the active site of the enzyme. B- When an enzyme catalyzes a reaction, the enzyme from the reaction must bind to the active site of the substrate. C- When an enzyme b ...
... ________ 37) Which of the following is accurate? A- When an enzyme catalyzes a reaction, the substrate from the reaction must bind to the active site of the enzyme. B- When an enzyme catalyzes a reaction, the enzyme from the reaction must bind to the active site of the substrate. C- When an enzyme b ...
Protein performance in emulsion stability
... In cases when there is insufficient solubilised protein available or protein with inadequate properties, the meat emulsion is weakened, and the fat expands during heating and subsequently breaks through the protein layer causing fat (and jelly) separation. The influence of fat in processed meat prod ...
... In cases when there is insufficient solubilised protein available or protein with inadequate properties, the meat emulsion is weakened, and the fat expands during heating and subsequently breaks through the protein layer causing fat (and jelly) separation. The influence of fat in processed meat prod ...
chapter 23
... of cellulose make up plant cell walls. More than 50% of the total organic matter in the world is cellulose. People cannot digest cellulose, but when we eat fiber, which is cellulose, it speeds the movement of food through the digestive tract. Microorganisms that can digest cellulose are present in t ...
... of cellulose make up plant cell walls. More than 50% of the total organic matter in the world is cellulose. People cannot digest cellulose, but when we eat fiber, which is cellulose, it speeds the movement of food through the digestive tract. Microorganisms that can digest cellulose are present in t ...
ATP - Mhanafi123`s Blog
... lactate include brain, gastrointestinal tract, renal medulla, retina, and skin. Lactate production is also increased in septic shock, and many cancers also produce lactate. ...
... lactate include brain, gastrointestinal tract, renal medulla, retina, and skin. Lactate production is also increased in septic shock, and many cancers also produce lactate. ...
Slide 1
... enzymes safely isolating them from the rest of the cytoplasm. -fuse to food vacuoles and digest the contents. -fuse to “captured” vacuoles and ...
... enzymes safely isolating them from the rest of the cytoplasm. -fuse to food vacuoles and digest the contents. -fuse to “captured” vacuoles and ...
lecture07_13
... For each position l in the input sequence, check if substring starting at position l matches the motif. Example: find the consensus motif NTAHAWT in the promoter of a gene >promoter of gene A ...
... For each position l in the input sequence, check if substring starting at position l matches the motif. Example: find the consensus motif NTAHAWT in the promoter of a gene >promoter of gene A ...
TD3 5.08 – PKS/NRPS pathways and mass
... - biosynthesizes yersiniabactin (Ybt), an Fe III chelator - Ybt strips Fe3+ from host proteins and is used for Y pestis survival - Ybt synthesized by hybrid NRPS/PKS system D= Dalton = g/mol ...
... - biosynthesizes yersiniabactin (Ybt), an Fe III chelator - Ybt strips Fe3+ from host proteins and is used for Y pestis survival - Ybt synthesized by hybrid NRPS/PKS system D= Dalton = g/mol ...
Elastin
... Elastin is an insoluble protein polymer synthesized from a precursor, TROPOELASTIN, which is a linear polypeptide composed of about 700 amino acids that are primarily small and nonpolar (for example, glycine, alanine, and valine). Tropoelastin is secreted by the cell into the extracellular space. Th ...
... Elastin is an insoluble protein polymer synthesized from a precursor, TROPOELASTIN, which is a linear polypeptide composed of about 700 amino acids that are primarily small and nonpolar (for example, glycine, alanine, and valine). Tropoelastin is secreted by the cell into the extracellular space. Th ...
Western blot
The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used to detect specific proteins in a sample of tissue homogenate or extract. It uses gel electrophoresis to separate native proteins by 3-D structure or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are stained with antibodies specific to the target protein. The gel electrophoresis step is included in western blot analysis to resolve the issue of the cross-reactivity of antibodies.There are many reagent companies that specialize in providing antibodies (both monoclonal and polyclonal antibodies) against tens of thousands of different proteins. Commercial antibodies can be expensive, although the unbound antibody can be reused between experiments. This method is used in the fields of molecular biology, immunogenetics and other molecular biology disciplines. A number of search engines, such as CiteAb, Antibodypedia, and SeekProducts, are available that can help researchers find suitable antibodies for use in western blotting.Other related techniques include dot blot analysis, immunohistochemistry and immunocytochemistry where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA).The method originated in the laboratory of Harry Towbin at the Friedrich Miescher Institute. The name western blot was given to the technique by W. Neal Burnette and is a play on the name Southern blot, a technique for DNA detection developed earlier by Edwin Southern. Detection of RNA is termed northern blot and was developed by George Stark at Stanford.