characterization of proteins from the cytoskeleton of giardia lamblia
... Proteins from the axonemes and disc cytoskeleton of Giardia lamblia have been examined by sodium dodecyl sulphate/polyacrylamide gel electrophoresis. In addition to tubulin and the 30 X 103 molecular weight disc protein, at least 18 minor components copurify with the two major proteins in Triton-ins ...
... Proteins from the axonemes and disc cytoskeleton of Giardia lamblia have been examined by sodium dodecyl sulphate/polyacrylamide gel electrophoresis. In addition to tubulin and the 30 X 103 molecular weight disc protein, at least 18 minor components copurify with the two major proteins in Triton-ins ...
nmr.evilia.190303
... The general formula for splitting is: # of lines in the multiplet (N)= 2nI + 1, where I is the spin of the neighboring nuclei and n is the number of identical nuclei. When I=1/2, this simplies to N=n+1. In structure work, J coupling is critical to measuring the bond angles and dihedral angles betwee ...
... The general formula for splitting is: # of lines in the multiplet (N)= 2nI + 1, where I is the spin of the neighboring nuclei and n is the number of identical nuclei. When I=1/2, this simplies to N=n+1. In structure work, J coupling is critical to measuring the bond angles and dihedral angles betwee ...
Sequence Variability Analysis of Human Class I and Class II MHC
... peptide binding, thymic repertoire bias and allograft rejection. While available 3D structural analysis suggests that polymorphisms are found primarily within the peptide-binding site, a broader informatic approach pinpointing functional polymorphisms relevant for immune recognition is currently lac ...
... peptide binding, thymic repertoire bias and allograft rejection. While available 3D structural analysis suggests that polymorphisms are found primarily within the peptide-binding site, a broader informatic approach pinpointing functional polymorphisms relevant for immune recognition is currently lac ...
Journal of Bacteriology
... and energy sources for growth and N2 fixation, whereas Azospirillum lipoferum can also use glucose (31). Recently, Azospirillum amazonense was described as a microaerobic, acid-tolerant, root-colonizing bacterium that can use sucrose to support growth and N2 fixation (8, 19). Biological N2 fixation ...
... and energy sources for growth and N2 fixation, whereas Azospirillum lipoferum can also use glucose (31). Recently, Azospirillum amazonense was described as a microaerobic, acid-tolerant, root-colonizing bacterium that can use sucrose to support growth and N2 fixation (8, 19). Biological N2 fixation ...
Understanding Tools and Techniques in Protein Structure Prediction
... available for protein structure visualization and protein identification and characterization. Thus bioinformatics tools for protein analysis provide a wealth of information related to sequences and structures of proteins. Use of computational tools is an essential kit for the biologist in this rapi ...
... available for protein structure visualization and protein identification and characterization. Thus bioinformatics tools for protein analysis provide a wealth of information related to sequences and structures of proteins. Use of computational tools is an essential kit for the biologist in this rapi ...
Characterisation and functional properties of watermelon (Citrullus
... The Osborne fractions of watermelon seeds had minimum solubility in the pH range 3–5 (data not shown). Protein solubility increased markedly below pH 3 and above pH 5. The considerable increase in protein solubility in acidic and alkaline environments was attributed to a gain in net negative or posi ...
... The Osborne fractions of watermelon seeds had minimum solubility in the pH range 3–5 (data not shown). Protein solubility increased markedly below pH 3 and above pH 5. The considerable increase in protein solubility in acidic and alkaline environments was attributed to a gain in net negative or posi ...
Journal of Agricultural and Food Chemistry
... fractions from soybean and amaranth. The protein fractions shared some common electrophoretic bands as well as a similar amino acid composition. The larger percent of denaturation in protein fractions, which is associated with enthalpy and the number of ruptured hydrogen bonds, corresponds to disapp ...
... fractions from soybean and amaranth. The protein fractions shared some common electrophoretic bands as well as a similar amino acid composition. The larger percent of denaturation in protein fractions, which is associated with enthalpy and the number of ruptured hydrogen bonds, corresponds to disapp ...
The role of multifunctional M1 metallopeptidases
... family of zinc metallopeptidases all share the catalytic region containing a signature amino acid exopeptidase (GXMXN) and a zinc binding (HEXXH[18X]E) motif. In addition, M1 aminopeptidases often also contain additional membrane association and/or protein interaction motifs. These protein interacti ...
... family of zinc metallopeptidases all share the catalytic region containing a signature amino acid exopeptidase (GXMXN) and a zinc binding (HEXXH[18X]E) motif. In addition, M1 aminopeptidases often also contain additional membrane association and/or protein interaction motifs. These protein interacti ...
Redacted for Privacy
... sequence of his-gly-val-cys-phe-ala-ser-met (HGVCFASM), with a farnesyl group linked via thioether linkage to the cysteine residue. Methods were developed for the concerted investigation of the transmethylglutaconate shunt, the isolation of mevalonate-2-'4C labeled peptides, and ...
... sequence of his-gly-val-cys-phe-ala-ser-met (HGVCFASM), with a farnesyl group linked via thioether linkage to the cysteine residue. Methods were developed for the concerted investigation of the transmethylglutaconate shunt, the isolation of mevalonate-2-'4C labeled peptides, and ...
A Quaternion-Based Definition of Protein
... and P-straightness measures for all within(helix), within(sheet), and (protein and not helix and not sheet) residues in the Protein Data Bank.] ...
... and P-straightness measures for all within(helix), within(sheet), and (protein and not helix and not sheet) residues in the Protein Data Bank.] ...
Structural Characterization of Humanized Nanobodies with
... nanobodies as a novel adjunctive anti-pertussis agent. Moreover, we have identified the region on Linker 1 connecting Blocks I and II within the CyaA-RTX subdomain that could be a potential neutralizing epitope of CyaA-protective antigen. 2. Results and Discussion 2.1. Isolated CyaA-Hly-Specific Nan ...
... nanobodies as a novel adjunctive anti-pertussis agent. Moreover, we have identified the region on Linker 1 connecting Blocks I and II within the CyaA-RTX subdomain that could be a potential neutralizing epitope of CyaA-protective antigen. 2. Results and Discussion 2.1. Isolated CyaA-Hly-Specific Nan ...
Multiple Sequence Motifs in the Rubisco Small
... identified sequence motifs in the E1a-tp critical for protein import into chloroplasts. Serial Ala scanning mutants, E1a[T2A], E1a[T3A], E1a[T4A], E1a[T5A], and E1a[T6A], were generated by replacing the second, third, fourth, fifth, and sixth 10-amino acid blocks, respectively, with Alas (Fig. 2A). ...
... identified sequence motifs in the E1a-tp critical for protein import into chloroplasts. Serial Ala scanning mutants, E1a[T2A], E1a[T3A], E1a[T4A], E1a[T5A], and E1a[T6A], were generated by replacing the second, third, fourth, fifth, and sixth 10-amino acid blocks, respectively, with Alas (Fig. 2A). ...
Application Note
... advanced. This highly reactive amine derivatization reagent can be used in an easy one step procedure. The compound reacts with amines through nucleophilic attack on the carbonyl carbon of AQC. This reaction results in the loss of N-hydroxysuccinimide (NHS) and CO2 (Fig. 1). Excess AQC is rapidly hy ...
... advanced. This highly reactive amine derivatization reagent can be used in an easy one step procedure. The compound reacts with amines through nucleophilic attack on the carbonyl carbon of AQC. This reaction results in the loss of N-hydroxysuccinimide (NHS) and CO2 (Fig. 1). Excess AQC is rapidly hy ...
Peer-reviewed Article PDF
... for protozoan cells proliferation and considered as essential nutrients for the protozoan parasites. For example, Neurospora crassa mutants lacking ODC activity cannot grow unless putrescine, spermidine or spermines were added to the growing media [12,13]. The protozoan pathogens, Trypanosomatids de ...
... for protozoan cells proliferation and considered as essential nutrients for the protozoan parasites. For example, Neurospora crassa mutants lacking ODC activity cannot grow unless putrescine, spermidine or spermines were added to the growing media [12,13]. The protozoan pathogens, Trypanosomatids de ...
amino acids M
... -Proteins with the same function from different organisms have similar primary structures -Amino-acid sequence determines the 3-D structure in which the protein folds and its biological function. -Proteins can have very different sizes (from 30 to ~ 100,000 residues) ...
... -Proteins with the same function from different organisms have similar primary structures -Amino-acid sequence determines the 3-D structure in which the protein folds and its biological function. -Proteins can have very different sizes (from 30 to ~ 100,000 residues) ...
PLP-dependent Enzymes: a Powerful Tool for - Beilstein
... solved in the last 5 years, are accessible from databases. Catalytic versatility and promiscuity Enzymes are generally believed to be very specific in their action, i. e. to be endowed with a strict reaction and substrate specificity. However, as a matter of fact, many enzymes are able to catalyze m ...
... solved in the last 5 years, are accessible from databases. Catalytic versatility and promiscuity Enzymes are generally believed to be very specific in their action, i. e. to be endowed with a strict reaction and substrate specificity. However, as a matter of fact, many enzymes are able to catalyze m ...
manualE6901
... a native recombinant protein without the use of a protease. Each intein tag contains a chitin binding domain (CBD) for the affinity purification of the fusion protein on a chitin resin (2-4). Induction of on-column cleavage, using thiol reagents such as dithiothreitol (DTT), releases the target prot ...
... a native recombinant protein without the use of a protease. Each intein tag contains a chitin binding domain (CBD) for the affinity purification of the fusion protein on a chitin resin (2-4). Induction of on-column cleavage, using thiol reagents such as dithiothreitol (DTT), releases the target prot ...
Determination of Protein Concentrations Using AAA
... AAA-Direct has many advantages over derivatization methods for AAA. The derivatized amino acid products may not be stable, depending on the derivatization method, and the reagents can cause interferences during chromatography. Lengthy sample preparation and derivatization steps are often needed. Der ...
... AAA-Direct has many advantages over derivatization methods for AAA. The derivatized amino acid products may not be stable, depending on the derivatization method, and the reagents can cause interferences during chromatography. Lengthy sample preparation and derivatization steps are often needed. Der ...
Chemical Classification of Cyclic Depsipeptides
... A third group of CDPs contains both α-hydroxy and β-hydroxy acids (Figure 9), of which the type of β-hydroxy acid is the next relevant feature for further classification, similar to the βhydroxy acids. Figure 9 shows their classification into two major groups which can be further divided into other ...
... A third group of CDPs contains both α-hydroxy and β-hydroxy acids (Figure 9), of which the type of β-hydroxy acid is the next relevant feature for further classification, similar to the βhydroxy acids. Figure 9 shows their classification into two major groups which can be further divided into other ...
ZRII ACHIEVE™ PRODUCT INFORMATION
... tannic acids, phytonutrients, trace minerals and other beneficial nutrients. Why did you include non-GMO soy protein in the product? Soy is naturally high in protein, rich in complex carbohydrates, high in fiber, and free of lactose, making it a good protein choice for many people. Achieve uses only ...
... tannic acids, phytonutrients, trace minerals and other beneficial nutrients. Why did you include non-GMO soy protein in the product? Soy is naturally high in protein, rich in complex carbohydrates, high in fiber, and free of lactose, making it a good protein choice for many people. Achieve uses only ...
Changes in chemical composition in male turkeys
... The viscera and feather contents generally decreased with increasing age (Table 1), resulting in an increase in carcass content from 75.2 to 90.2% of BW between 1 and 15 wk. These tendencies are also reflected in the scale parameter (b) of equation 2b, which was greater than unity for the carcass an ...
... The viscera and feather contents generally decreased with increasing age (Table 1), resulting in an increase in carcass content from 75.2 to 90.2% of BW between 1 and 15 wk. These tendencies are also reflected in the scale parameter (b) of equation 2b, which was greater than unity for the carcass an ...