REDESIGN OF CARNITINE ACETYLTRANSFERASE SPECIFICITY BY PROTEIN ENGINEERING UNIVERSIDAD DE BARCELONA
... EST sequences from rat CrAT, and no such amino acid sequence can be aligned with protein sequences of CPT I, CPT II or COT from various organisms. Alternatively, if such an exon were to exist, expression of the processed mRNA containing exon 7bis in rat testis would be undetectable, even using PCR a ...
... EST sequences from rat CrAT, and no such amino acid sequence can be aligned with protein sequences of CPT I, CPT II or COT from various organisms. Alternatively, if such an exon were to exist, expression of the processed mRNA containing exon 7bis in rat testis would be undetectable, even using PCR a ...
http://doc.rero.ch
... solution once a cation is formed (it is assumed to still be a stable PP-II helix) and about the exact mechanism of ET. Direct through-space ET via vacuum tunneling between donor and acceptor is strongly distance dependent and not relevant in the case here, where a condensed medium (both peptide and ...
... solution once a cation is formed (it is assumed to still be a stable PP-II helix) and about the exact mechanism of ET. Direct through-space ET via vacuum tunneling between donor and acceptor is strongly distance dependent and not relevant in the case here, where a condensed medium (both peptide and ...
Safety Assessment of Soy Proteins and Peptides as Used in
... 71 kDa) and β (ca 50 kDa). The 11S globulin is a hexamer, and is made up of five different subunits, each of which consists of an acidic subunit A (ca 35 kDa) and a basic subunit B (ca 20 kDa), linked by a disulfide bond. The 11S globulin was found to dissociate into 2S, 3S or 7S forms in solutions ...
... 71 kDa) and β (ca 50 kDa). The 11S globulin is a hexamer, and is made up of five different subunits, each of which consists of an acidic subunit A (ca 35 kDa) and a basic subunit B (ca 20 kDa), linked by a disulfide bond. The 11S globulin was found to dissociate into 2S, 3S or 7S forms in solutions ...
Candida antarctica Anders G. Sandström
... crystals of sodium ammonium tartrate; the crystal shapes were mirrorimages of each other.15 Enantiomers have the same physical properties provided that they are in an achiral environment. Biological organisms contain a large quantity of enantiopure molecules, and therefore constitute chiral environm ...
... crystals of sodium ammonium tartrate; the crystal shapes were mirrorimages of each other.15 Enantiomers have the same physical properties provided that they are in an achiral environment. Biological organisms contain a large quantity of enantiopure molecules, and therefore constitute chiral environm ...
Classification and Phylogenetic Analysis of the cAMP
... classified accordingly. DNA sequences were translated with the Translate utility at Expasy (http://www.expasy.ch). Multiple Sequence Alignment. Multiple sequence alignments were performed using ClustalW 1.6 (Thompson et al. 1994). Priority was set to placing gaps within loops connecting secondary st ...
... classified accordingly. DNA sequences were translated with the Translate utility at Expasy (http://www.expasy.ch). Multiple Sequence Alignment. Multiple sequence alignments were performed using ClustalW 1.6 (Thompson et al. 1994). Priority was set to placing gaps within loops connecting secondary st ...
Modeling Multi-typed Structurally Viewed Chemicals with the UMLS
... hydrolysis reaction) that undoes the original reaction used in the conjugate’s creation. On the other hand, mixtures are made of two or more chemicals, where the chemicals are not joined by covalent bonds. Therefore, they can be mixed at different proportions (i.e., the composition can be varied). W ...
... hydrolysis reaction) that undoes the original reaction used in the conjugate’s creation. On the other hand, mixtures are made of two or more chemicals, where the chemicals are not joined by covalent bonds. Therefore, they can be mixed at different proportions (i.e., the composition can be varied). W ...
A first attempt to elucidate the amino acid sequence of some lichen
... The lowest content of Val was found in SAX. SAX contained lower amounts of Ile, Leu and Lys, and higher amounts of His, than SAE. Substantial differences in the amino acid composition of the purified lectins were expected for SAE, SAX, and Dictyonema glabratum (Atheliaceae) on the basis of their ...
... The lowest content of Val was found in SAX. SAX contained lower amounts of Ile, Leu and Lys, and higher amounts of His, than SAE. Substantial differences in the amino acid composition of the purified lectins were expected for SAE, SAX, and Dictyonema glabratum (Atheliaceae) on the basis of their ...
Can the Origin of the Genetic Code Be Explained - BIO
... affinities. To do this, they looked for RNA strands that bound certain amino acids preferentially, from a class of RNA molecules now dubbed aptamers. Using the directed evolution method SELEX, in which large pools of random RNA strands are synthesized and then sifted for particular functions (in thi ...
... affinities. To do this, they looked for RNA strands that bound certain amino acids preferentially, from a class of RNA molecules now dubbed aptamers. Using the directed evolution method SELEX, in which large pools of random RNA strands are synthesized and then sifted for particular functions (in thi ...
Essential amino acid
... Isoleucine, Leucine, and Valine: These hydrophobic amino acids are essential for the production and maintenance of body proteins. Lysine: Deficiency can lead to poor appetite, reduction in body weight, anemia, and a reduced ability to concentrate, as well as pneumonia, kidney disease (nephritis), an ...
... Isoleucine, Leucine, and Valine: These hydrophobic amino acids are essential for the production and maintenance of body proteins. Lysine: Deficiency can lead to poor appetite, reduction in body weight, anemia, and a reduced ability to concentrate, as well as pneumonia, kidney disease (nephritis), an ...
Assessment of the mathematical issues involved
... administering a large quantity of a specific amino acid on protein synthesis is a difficult task. There is a large body of literature, which has recently been reviewed in [4], indicating that leucine flooding doses promote protein synthesis in vitro when other amino acids are plentiful. One should b ...
... administering a large quantity of a specific amino acid on protein synthesis is a difficult task. There is a large body of literature, which has recently been reviewed in [4], indicating that leucine flooding doses promote protein synthesis in vitro when other amino acids are plentiful. One should b ...
BMC Struct Biol. BMC Struct Biol. 2003
... acid binding to site 2' and indeed the density for the fatty acid bound to site 2 is relatively weak, probably as a result of the reduced occupancy (Figure (Figure2c).2c). Thus it may be that binding to site 2' is required to drive the more extensive conformational change between domains I and II. A ...
... acid binding to site 2' and indeed the density for the fatty acid bound to site 2 is relatively weak, probably as a result of the reduced occupancy (Figure (Figure2c).2c). Thus it may be that binding to site 2' is required to drive the more extensive conformational change between domains I and II. A ...
Responses of Growing Broilers to Diets with Increased Sulfur Amino
... have to be determined only for lysine, which is usually taken as the reference amino acid, and optimum levels for the rest of the essential amino acids are obtained simply by using the respective optimum ratios. Several experimental methods are available to determine the ideal amino acid ratios as s ...
... have to be determined only for lysine, which is usually taken as the reference amino acid, and optimum levels for the rest of the essential amino acids are obtained simply by using the respective optimum ratios. Several experimental methods are available to determine the ideal amino acid ratios as s ...
Amino Acid Cost and Codon-Usage Biases in 6 Prokaryotic
... against all other proteins from that organism. Proteins with greater than 60% amino acid identity over a stretch of more than 60 amino acids in these intraorganismal searches were considered to be paralogous. This approach made our results directly comparable to Akashi and Gojobori (2002). Only the ...
... against all other proteins from that organism. Proteins with greater than 60% amino acid identity over a stretch of more than 60 amino acids in these intraorganismal searches were considered to be paralogous. This approach made our results directly comparable to Akashi and Gojobori (2002). Only the ...
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... studies used polymers, such as polyethylenimine with electron-donating character [13] and nitrile functionalized polyimides with electron-withdrawing character, to probe charge transfer interactions with the SWCNT surface [15]. Amphiphilic peptides containing aromatic moieties are also known to alte ...
... studies used polymers, such as polyethylenimine with electron-donating character [13] and nitrile functionalized polyimides with electron-withdrawing character, to probe charge transfer interactions with the SWCNT surface [15]. Amphiphilic peptides containing aromatic moieties are also known to alte ...
AmdurskyN-ChemPhysChem-2015-accepted-version
... the concentration of 8 mg mL−1) within wavelengths of 210–212 nm. The large redshift with respect to group (2) is attributed to the charged amine groups. Our results (the 8 mg mL−1 curve) of Lys are in line with previous reported spectra of Lys.4c Amino acids with negatively electrically charged sid ...
... the concentration of 8 mg mL−1) within wavelengths of 210–212 nm. The large redshift with respect to group (2) is attributed to the charged amine groups. Our results (the 8 mg mL−1 curve) of Lys are in line with previous reported spectra of Lys.4c Amino acids with negatively electrically charged sid ...
Document
... To study proteins is to study diversity! i.e., diversity of structure, function, chemistry, analysis, etc. To emphasize the scope of diversity, let us focus on structural diversity... • Structure is a shape, sequence, order, orientation, configuration, etc. of an atom or molecule. • Eg. The electro ...
... To study proteins is to study diversity! i.e., diversity of structure, function, chemistry, analysis, etc. To emphasize the scope of diversity, let us focus on structural diversity... • Structure is a shape, sequence, order, orientation, configuration, etc. of an atom or molecule. • Eg. The electro ...
Statistical Analysis of Amino Acid Patterns in Transmembrane
... database of transmembrane domains. Our analysis was performed with a novel formalism, which we call TMSTAT, for exactly calculating the expectancies of all pairs and triplets of residues in individual sequences, taking into account differential sequence composition and the substantial effect of ®nit ...
... database of transmembrane domains. Our analysis was performed with a novel formalism, which we call TMSTAT, for exactly calculating the expectancies of all pairs and triplets of residues in individual sequences, taking into account differential sequence composition and the substantial effect of ®nit ...
Derivation and testing of pair potentials for protein folding. When is
... many proteins from the random state. Over the years, a variety of amino acid pair-specific potentials have been formulated (Miyazawa & Jernigan, 1985, 1996). By way of illustration, we focus on potentials that are contact based, i.e., where an interaction between two residues is allowed if their dis ...
... many proteins from the random state. Over the years, a variety of amino acid pair-specific potentials have been formulated (Miyazawa & Jernigan, 1985, 1996). By way of illustration, we focus on potentials that are contact based, i.e., where an interaction between two residues is allowed if their dis ...
Comparison of the Structure of the Extrinsic 33 kDa Protein from
... combined to yield the whole sequence of the gene, which is shown in Fig. 1. This sequence contained two in-frame ATG codons upstream of the N-terminal leucine residue of the mature protein; they are located at positions 100 and 193, respectively. Among them, the ATG codon at nucleotide number 100 wa ...
... combined to yield the whole sequence of the gene, which is shown in Fig. 1. This sequence contained two in-frame ATG codons upstream of the N-terminal leucine residue of the mature protein; they are located at positions 100 and 193, respectively. Among them, the ATG codon at nucleotide number 100 wa ...
Science
... The PGC-1a–K778 peptide contains aromatic, hydrophobic amino acids at the +1 and +6 positions (relative to the acetylated lysine), as does the FOXO3a-K290 peptide at position +1. Alanine substitution of either the Tyr at the +1 position or the Phe at the +6 position of the PGC1a peptide reduced acti ...
... The PGC-1a–K778 peptide contains aromatic, hydrophobic amino acids at the +1 and +6 positions (relative to the acetylated lysine), as does the FOXO3a-K290 peptide at position +1. Alanine substitution of either the Tyr at the +1 position or the Phe at the +6 position of the PGC1a peptide reduced acti ...
Carotenoids Biosynthesis – a review
... Carotenoids are naturally occurring pigments synthesized as hydrocarbons (carotenes) and their oxygenated derivatives (xanthophylls) by plants and microorganisms. They consist of eight isoprenoid units joined in such a manner that the arrangement of isoprenoid units is reversed at the center of the ...
... Carotenoids are naturally occurring pigments synthesized as hydrocarbons (carotenes) and their oxygenated derivatives (xanthophylls) by plants and microorganisms. They consist of eight isoprenoid units joined in such a manner that the arrangement of isoprenoid units is reversed at the center of the ...
Document
... preserves the enzymes in a temporarily inactive state; where as high temperature destroys enzymatic activity because proteins are denatured by heat. b. Concentration of substrate. With the increase in substrate concentration, the velocity of the enzymatic reaction rises at first. The reaction ultima ...
... preserves the enzymes in a temporarily inactive state; where as high temperature destroys enzymatic activity because proteins are denatured by heat. b. Concentration of substrate. With the increase in substrate concentration, the velocity of the enzymatic reaction rises at first. The reaction ultima ...
Protein Sequences in the CAS Registry File on
... These codes are used for displaying or searching protein sequences with chain lengths of four or more. Dipeptides and tripeptides are also included in REGISTRY, but may be searched only by name or structure and not by sequence representation. For common amino acids, either one-letter or threeletter ...
... These codes are used for displaying or searching protein sequences with chain lengths of four or more. Dipeptides and tripeptides are also included in REGISTRY, but may be searched only by name or structure and not by sequence representation. For common amino acids, either one-letter or threeletter ...