Active site mapping, biochemical properties and
... Trypanosoma brucei rhodesiense (MVAT4 strain), in reagent quantities from Pichia pastoris. Rhodesain was secreted as an active, mature protease. Site-directed mutagenesis of a cryptic glycosylation motif not previously identified allowed production of rhodesain suitable for crystallization. An invar ...
... Trypanosoma brucei rhodesiense (MVAT4 strain), in reagent quantities from Pichia pastoris. Rhodesain was secreted as an active, mature protease. Site-directed mutagenesis of a cryptic glycosylation motif not previously identified allowed production of rhodesain suitable for crystallization. An invar ...
Case Study 5 Literature - Department of Chemistry
... GAPDH from T. tenax—The sequence analysis revealed a single open reading frame comprising 1503 base pairs (Fig. 1, positions 130 –1632) corresponding to a polypeptide of 501 amino acid residues with a calculated molecular mass of 55 kDa. The deduced amino acid sequence corresponds with the partial a ...
... GAPDH from T. tenax—The sequence analysis revealed a single open reading frame comprising 1503 base pairs (Fig. 1, positions 130 –1632) corresponding to a polypeptide of 501 amino acid residues with a calculated molecular mass of 55 kDa. The deduced amino acid sequence corresponds with the partial a ...
Engineering of cyclodextrin glycosyltransferase reaction and
... resulted in loss of both activities [29]. The amylopullulanase from alkalophilic Bacillus sp. KSM-1378, however, contains two independent active sites [30,31] and the speci¢c hydrolytic activities can be separated by limited proteolysis with papain, yielding two protein fragments of which one has th ...
... resulted in loss of both activities [29]. The amylopullulanase from alkalophilic Bacillus sp. KSM-1378, however, contains two independent active sites [30,31] and the speci¢c hydrolytic activities can be separated by limited proteolysis with papain, yielding two protein fragments of which one has th ...
STING Millennium: a web-based suite of programs
... 8. cumulative statistics on protein family characteristics. All these activities require an integrated environment with computational tools that can visually and schematically map 3D information together with parameters and the patterns extracted from a number of databases, on the protein sequence. ...
... 8. cumulative statistics on protein family characteristics. All these activities require an integrated environment with computational tools that can visually and schematically map 3D information together with parameters and the patterns extracted from a number of databases, on the protein sequence. ...
Unusual properties of the fungal conventional kinesin neck domain
... 1996), but experimental evidence has been lacking so far. We therefore synthesized a NcKin neck domain peptide comprising amino acids 338±379 (peptide Kn1, Figure 1), which exceeds the expected coiled-coil by ®ve amino acid residues at both the N- and C-termini. The solubility and conformational sta ...
... 1996), but experimental evidence has been lacking so far. We therefore synthesized a NcKin neck domain peptide comprising amino acids 338±379 (peptide Kn1, Figure 1), which exceeds the expected coiled-coil by ®ve amino acid residues at both the N- and C-termini. The solubility and conformational sta ...
Tunicates and not cephalochordates are the closest living relatives
... test a number of evolutionary hypotheses through the analysis of individual genes such as ribosomal RNA (rRNA). However, phylogenies reconstructed from single or a small number of genes are hampered by stochastic effects limiting the statistical significance of the results. The genomic era is now pr ...
... test a number of evolutionary hypotheses through the analysis of individual genes such as ribosomal RNA (rRNA). However, phylogenies reconstructed from single or a small number of genes are hampered by stochastic effects limiting the statistical significance of the results. The genomic era is now pr ...
ref. #27 of the TIBS article
... the microsomal in vitro system (Mothes et al., 1997), we also wanted to test the relation between MGD and n for a protein that does not use this pathway. Synaptobrevin is known to insert a hydrophobic C-terminal segment into the microsomal membrane by an as yet poorly understood mechanism that does ...
... the microsomal in vitro system (Mothes et al., 1997), we also wanted to test the relation between MGD and n for a protein that does not use this pathway. Synaptobrevin is known to insert a hydrophobic C-terminal segment into the microsomal membrane by an as yet poorly understood mechanism that does ...
Methodology for predicting semantic annotations of protein
... The framework of this thesis is located in the field of bioinformatics. Particularly in the area associated to machine learning and pattern recognition, where a methodology for predict semantic annotations of proteins based on a robust feature extraction is proposed. The first part of this thesis co ...
... The framework of this thesis is located in the field of bioinformatics. Particularly in the area associated to machine learning and pattern recognition, where a methodology for predict semantic annotations of proteins based on a robust feature extraction is proposed. The first part of this thesis co ...
Cloning and sequencing of a gene encoding acidophilic amylase
... conditions (data not shown). Therefore, the 160 kDa form was apparently not a disulphide-linkeddimer of the 90 kDa form. The amount of protein in the 90 kDa and 160 kDa bands was approximately the same, yet the starch-degrading activity of the 160 kDa protein was much higher (Fig. 1). Thus, the 160 ...
... conditions (data not shown). Therefore, the 160 kDa form was apparently not a disulphide-linkeddimer of the 90 kDa form. The amount of protein in the 90 kDa and 160 kDa bands was approximately the same, yet the starch-degrading activity of the 160 kDa protein was much higher (Fig. 1). Thus, the 160 ...
The CamSol Method of Rational Design of Protein Mutants with
... thousands of amino acid substitutions or insertions to identify specific mutations that are predicted to maximally increase the solubility of a protein while preserving its fundamental properties, including its functional structure and binding affinity. The method requires the knowledge of the nativ ...
... thousands of amino acid substitutions or insertions to identify specific mutations that are predicted to maximally increase the solubility of a protein while preserving its fundamental properties, including its functional structure and binding affinity. The method requires the knowledge of the nativ ...
Directed mutagenesis of the Trypanosoma cruzi trans
... protein with sialidase and sialyltransferase activities. The sialidase activity of this mutant exhibited the same pH sensitivity as the pS3-2 encoded product (not shown). Its level of sialyltransferase activity ranged from 12 to 25% that of the wild type T.cruzi TS enzyme. To determine if any of the ...
... protein with sialidase and sialyltransferase activities. The sialidase activity of this mutant exhibited the same pH sensitivity as the pS3-2 encoded product (not shown). Its level of sialyltransferase activity ranged from 12 to 25% that of the wild type T.cruzi TS enzyme. To determine if any of the ...
End-product control of enzymes of branched
... many provisos, one of which is that feedback effects on enzyme activity may be playing a role. To examine this possibility, cultures were grown without branched-chain amino acid supplementation, in the expectation of removing any amino acids bound to the enzymes. Mycelial extracts were assayed in re ...
... many provisos, one of which is that feedback effects on enzyme activity may be playing a role. To examine this possibility, cultures were grown without branched-chain amino acid supplementation, in the expectation of removing any amino acids bound to the enzymes. Mycelial extracts were assayed in re ...
Do asparagine-linked carbohydrate chains in glycoproteins have a
... Although current nucleic acid sequencing generally leads more rapidly to knowledge of the primary structure of a protein than classical protein sequencing, the latter remains essential to determine posttranslational modifications of the primary sequences. One of the most frequently occurring modific ...
... Although current nucleic acid sequencing generally leads more rapidly to knowledge of the primary structure of a protein than classical protein sequencing, the latter remains essential to determine posttranslational modifications of the primary sequences. One of the most frequently occurring modific ...
Molecular Characterization of a Hamster Oviduct
... BabOGP cDNA probe [20]. The BabOGP cDNA was partially amplified by the polymerase chain reaction (PCR) in the presence of DIG-11-dUTP. Based on the DNA sequence data described by Donnelly et al. [20], two oligonucleotides (a part of the sense or anti-sense sequence of a baboon oviduct-specific glyco ...
... BabOGP cDNA probe [20]. The BabOGP cDNA was partially amplified by the polymerase chain reaction (PCR) in the presence of DIG-11-dUTP. Based on the DNA sequence data described by Donnelly et al. [20], two oligonucleotides (a part of the sense or anti-sense sequence of a baboon oviduct-specific glyco ...
Document
... clear evidence that SL is involved in energy homeostasis, lipid metabolism and possibly growth. Insulin-like growth factors (IGFs) are mitogenic peptide hormones produced by all known vertebrates, mainly in the liver. Two separate IGFs (IGF-I and IGF-II) have been identified in bony fish and have be ...
... clear evidence that SL is involved in energy homeostasis, lipid metabolism and possibly growth. Insulin-like growth factors (IGFs) are mitogenic peptide hormones produced by all known vertebrates, mainly in the liver. Two separate IGFs (IGF-I and IGF-II) have been identified in bony fish and have be ...
¹⁵N Heteronuclear Single Quantum Coherence
... hemoglobin α- and β-subunits are too distant to be covalently linked by a short (i.e., <8 residues) peptide (Shaanan, 1983). To generate a single-chain hemoglobin we have chosen to relocate the β-subunit termini by circular permutation, to allow for novel covalent connections between subunits using ...
... hemoglobin α- and β-subunits are too distant to be covalently linked by a short (i.e., <8 residues) peptide (Shaanan, 1983). To generate a single-chain hemoglobin we have chosen to relocate the β-subunit termini by circular permutation, to allow for novel covalent connections between subunits using ...
Citrate synthase proteins in extremophilic organisms: Studies within
... examine thermal stability, local flexibility, collective motions, and unfolding kinetics of CS enzymes functioning in organisms that live in very different environmental conditions. The adaptations of life to different environmental conditions can be observed at various levels of organization, includ ...
... examine thermal stability, local flexibility, collective motions, and unfolding kinetics of CS enzymes functioning in organisms that live in very different environmental conditions. The adaptations of life to different environmental conditions can be observed at various levels of organization, includ ...
Unconventional serine proteases: Variations on the catalytic Ser/His
... share the same protein fold and have their catalytic residues in the order of His/Asp/Ser from the N to C terminus. Their active site regions are composed of (1) the substrate binding groove where nonspecific mainchain hydrogen bond interactions occur between the enzyme and substrate, (2) the substr ...
... share the same protein fold and have their catalytic residues in the order of His/Asp/Ser from the N to C terminus. Their active site regions are composed of (1) the substrate binding groove where nonspecific mainchain hydrogen bond interactions occur between the enzyme and substrate, (2) the substr ...
Comparative analysis of proteins with a mucus
... more similar to each other than to the MUB domains in other proteins of the same species. This suggests that the introduction of multiple copies of the domain in a single protein occurred after speciation. In a few proteins, two different versions of the MUB domain can be distinguished; experiments ...
... more similar to each other than to the MUB domains in other proteins of the same species. This suggests that the introduction of multiple copies of the domain in a single protein occurred after speciation. In a few proteins, two different versions of the MUB domain can be distinguished; experiments ...
Studying Protein Flexibility in a Statistical Framework: Tools and
... motions and get a sense of the full size the motions “universe.” There are currently 13191 entries in the NCBI's PubMed database that contain phrases such as “conformational change” or “macromolecular motion.” The increase of these terms over the years is diagrammed in Figure 5 and the search method ...
... motions and get a sense of the full size the motions “universe.” There are currently 13191 entries in the NCBI's PubMed database that contain phrases such as “conformational change” or “macromolecular motion.” The increase of these terms over the years is diagrammed in Figure 5 and the search method ...
embor2011116-sup-0001
... proteins that are most important for promoting the formation of amyloid aggregates. It uses the Dubay et al. algorithm, but considers only the intrinsic factors (charge, hydrophobicity and PATs) to calculate the amyloid aggregation propensities for all the naturally occurring amino acid residues. Wh ...
... proteins that are most important for promoting the formation of amyloid aggregates. It uses the Dubay et al. algorithm, but considers only the intrinsic factors (charge, hydrophobicity and PATs) to calculate the amyloid aggregation propensities for all the naturally occurring amino acid residues. Wh ...
Reactive cysteine in proteins: Protein folding - Genoma
... as thiol/disulfide oxido-reductases. Thioredoxin appears to be a very ancient protein since it is widespread among all the living organisms. These small proteins (12–13 kDa) possess disulfide reductase activity endowed by two vicinal cysteines present in a CXXC motif (typically CGPC), which are used ...
... as thiol/disulfide oxido-reductases. Thioredoxin appears to be a very ancient protein since it is widespread among all the living organisms. These small proteins (12–13 kDa) possess disulfide reductase activity endowed by two vicinal cysteines present in a CXXC motif (typically CGPC), which are used ...