Multiple linear regression for protein secondary structure prediction
... Prediction of the three-dimensional structure of a protein from its sequence is becoming a pressing problem for many biologists because the discrepancy continues to increase between the number of known protein sequences and the number of experimentally determined structures. The prediction of second ...
... Prediction of the three-dimensional structure of a protein from its sequence is becoming a pressing problem for many biologists because the discrepancy continues to increase between the number of known protein sequences and the number of experimentally determined structures. The prediction of second ...
Single-Amino Acid Substitutions Alter the Specificity and Affinity of
... Cyclic peptides containing an internal PDZ recognition motif that bind to syntrophin PDZ domains may adopt a similar conformation, since a constrained structure induced by disulfide-bonded cysteine residues is necessary for their interaction (13). In this report, we have generated recombinant PDZ do ...
... Cyclic peptides containing an internal PDZ recognition motif that bind to syntrophin PDZ domains may adopt a similar conformation, since a constrained structure induced by disulfide-bonded cysteine residues is necessary for their interaction (13). In this report, we have generated recombinant PDZ do ...
a-Aminoadipate aminotransferase from an extremely
... analysis of purified LysN was performed with a Beckman Optima XL-A analytical ultracentrifuge fitted with a Beckman An-60Ti analytical rotor. The subunit organization of the enzyme was analysed according to the procedure of Van Holde & Baldwin (1958). Enzyme assays. Enzyme assays were carried out ac ...
... analysis of purified LysN was performed with a Beckman Optima XL-A analytical ultracentrifuge fitted with a Beckman An-60Ti analytical rotor. The subunit organization of the enzyme was analysed according to the procedure of Van Holde & Baldwin (1958). Enzyme assays. Enzyme assays were carried out ac ...
Amino acid residues that determine functional specificity of NADP
... Apart from 344Lys, 345Tyr, and 351Val, several other amino acids contact cofactor in most considered structures: substrate-specific residues 103Leu, 105Thr, 337Ala, and 341Thr contact the nicotinamide nucleotide and thus spatially lie between the cofactor-binding and the substratebinding pockets. The ...
... Apart from 344Lys, 345Tyr, and 351Val, several other amino acids contact cofactor in most considered structures: substrate-specific residues 103Leu, 105Thr, 337Ala, and 341Thr contact the nicotinamide nucleotide and thus spatially lie between the cofactor-binding and the substratebinding pockets. The ...
Metabolismo dos aminoácidos e proteínas. II. Anabolismo
... Mutants of Aspergillus nidulans lacking NADP-GDH activity grow more poorly than wild-type strains on ammonium as a sole nitrogen source. The leaky growth of these mutants is indicative of an alternative pathway of ammonium assimilation and glutamate biosynthesis. A. nidulans mutants disrupted in th ...
... Mutants of Aspergillus nidulans lacking NADP-GDH activity grow more poorly than wild-type strains on ammonium as a sole nitrogen source. The leaky growth of these mutants is indicative of an alternative pathway of ammonium assimilation and glutamate biosynthesis. A. nidulans mutants disrupted in th ...
COMBINATION COMPRISING AT LEAST ONE AMINO ACID AND A
... acid supplementation, in an attempt to increase protein synthesis. The three BCAAs are valine, leucine, and isoleucine. : Previously, leucine has been shown to function, not only as a protein building block, but also as an inducer of signal transduction pathways that modulate translation initiation. ...
... acid supplementation, in an attempt to increase protein synthesis. The three BCAAs are valine, leucine, and isoleucine. : Previously, leucine has been shown to function, not only as a protein building block, but also as an inducer of signal transduction pathways that modulate translation initiation. ...
SURVEY AND SUMMARY Origins of tmRNA: the
... associated with Small protein B (SmpB). tmRNA is a hybrid molecule carrying out both transfer and messenger RNA activities, and its total length varies between about 260 and 430 nucleotides, depending on the cell species (Figure 4). It is always aminoacylated by alanine. As for SmpB, its topology ma ...
... associated with Small protein B (SmpB). tmRNA is a hybrid molecule carrying out both transfer and messenger RNA activities, and its total length varies between about 260 and 430 nucleotides, depending on the cell species (Figure 4). It is always aminoacylated by alanine. As for SmpB, its topology ma ...
Novel evolutionary lineages of the invertebrate oxytocin/vasopressin
... The common octopus, Octopus vulgaris, is the first invertebrate species that was shown to possess two oxytocin/vasopressin (OT/ VP) superfamily peptides, octopressin (OP) and cephalotocin (CT). Previously, we cloned a GPCR (G-protein-coupled receptor) specific to CT [CTR1 (CT receptor 1)]. In the pr ...
... The common octopus, Octopus vulgaris, is the first invertebrate species that was shown to possess two oxytocin/vasopressin (OT/ VP) superfamily peptides, octopressin (OP) and cephalotocin (CT). Previously, we cloned a GPCR (G-protein-coupled receptor) specific to CT [CTR1 (CT receptor 1)]. In the pr ...
the incorporation of c from sodium acetate- 2
... of minced mouse brain taken from the day-old mouse. They found to their surprise that 14C from uniformly labelled glucose was incorporated into all the amino acids, both essential and non-essential for the mouse, except threonine and proline (Rafelson et al. 1951). Following up this discovery they s ...
... of minced mouse brain taken from the day-old mouse. They found to their surprise that 14C from uniformly labelled glucose was incorporated into all the amino acids, both essential and non-essential for the mouse, except threonine and proline (Rafelson et al. 1951). Following up this discovery they s ...
pH-induced conformational changes in human ABO(H) blood group
... chosen for this study as it was known to have loop stability intermediate between GTA and GTB (Johal et al. 2012). In all 12 structures to which galactose was added regardless of the pH, a galactose molecule is present in the acceptorbinding site. UDP is also bound to the donor-binding site, althoug ...
... chosen for this study as it was known to have loop stability intermediate between GTA and GTB (Johal et al. 2012). In all 12 structures to which galactose was added regardless of the pH, a galactose molecule is present in the acceptorbinding site. UDP is also bound to the donor-binding site, althoug ...
Identifying producers of antibacterial compounds by
... At the same time that the pharmaceutical sector is abandoning natural products for antibiotics and other clinical indications, recently available genome sequences from multiple bacterial and environmental metagenomes have revealed a remarkably rich potential for the production of natural products4,5 ...
... At the same time that the pharmaceutical sector is abandoning natural products for antibiotics and other clinical indications, recently available genome sequences from multiple bacterial and environmental metagenomes have revealed a remarkably rich potential for the production of natural products4,5 ...
Characterization of Low Molecular Weight Glutenin Subunits by
... for LMW-glutenin subunits. Also present were sequences that showed a close correspondence to those derived from DNA sequences (NH 2-MetGlu-Thr-Ser-Cys-. . .), although the characteristic cysteine was not always present at position 5. C-type N-terminal sequences corresponded mainly to those associate ...
... for LMW-glutenin subunits. Also present were sequences that showed a close correspondence to those derived from DNA sequences (NH 2-MetGlu-Thr-Ser-Cys-. . .), although the characteristic cysteine was not always present at position 5. C-type N-terminal sequences corresponded mainly to those associate ...
Descriptions of translation related genes that
... SS (TAE2) Structural constituent of ribosome, has similarity with E. coli S8 and rat S15a MS (TAE4) ribosomal proteins VS Protein component of the small (40S) ribosomal subunit; nearly identical to Rps27Bp and has similarity to rat S27 ribosomal protein SS Ribosomal protein 10 (rp10) of the small (4 ...
... SS (TAE2) Structural constituent of ribosome, has similarity with E. coli S8 and rat S15a MS (TAE4) ribosomal proteins VS Protein component of the small (40S) ribosomal subunit; nearly identical to Rps27Bp and has similarity to rat S27 ribosomal protein SS Ribosomal protein 10 (rp10) of the small (4 ...
The Implausibility of Metabolic Cycles on the
... of reactants to products without itself being changed almost guarantees that a catalyst can initiate successive “cycles” of the same reaction. Metabolic cycles are different. Strictly, they are by definition restricted to biochemistry. Like catalytic cycles, they too result in repeated conversions of ...
... of reactants to products without itself being changed almost guarantees that a catalyst can initiate successive “cycles” of the same reaction. Metabolic cycles are different. Strictly, they are by definition restricted to biochemistry. Like catalytic cycles, they too result in repeated conversions of ...
Amino Acid Analysis Amino acid analysis refers to the methodology
... Hydrolysis of protein and peptide samples is necessary for amino acid analysis of these molecules. The glassware used for hydrolysis must be very clean to avoid erroneous results. Glove powders and fingerprints on hydrolysis tubes may cause contamination. To clean glass hydrolysis tubes, boil tubes ...
... Hydrolysis of protein and peptide samples is necessary for amino acid analysis of these molecules. The glassware used for hydrolysis must be very clean to avoid erroneous results. Glove powders and fingerprints on hydrolysis tubes may cause contamination. To clean glass hydrolysis tubes, boil tubes ...
(A6103) - Datasheet - Sigma
... aprotinin after heating to 60–80 °C. The high basicity of aprotinin causes it to adhere to commonly used dialysis tubing and even gel filtration matrices, but the use of acetylated materials and concentrated salt solutions (e.g., ≥0.1 M NaCl in buffer) minimizes this problem.5 Sterilization may be a ...
... aprotinin after heating to 60–80 °C. The high basicity of aprotinin causes it to adhere to commonly used dialysis tubing and even gel filtration matrices, but the use of acetylated materials and concentrated salt solutions (e.g., ≥0.1 M NaCl in buffer) minimizes this problem.5 Sterilization may be a ...
Publication: Amino acid profile of organically grown alternative
... artichoke originated in the American continent and has been first, a source for human food, and secondly, a source of animal food (Ben Chekroun et al., 1996). The main component of tubers’ dry matter content are carbohydrates and the greatest part of these consist of water soluble inulin (concentrat ...
... artichoke originated in the American continent and has been first, a source for human food, and secondly, a source of animal food (Ben Chekroun et al., 1996). The main component of tubers’ dry matter content are carbohydrates and the greatest part of these consist of water soluble inulin (concentrat ...
The exam is worth 200 points, divided into 7 questions. You must do
... (d) (5 pts) Indicate the positions of chymotrypsin cleavage, CNBr cleavage, and cleavage of the reduced and modified peptides with trypsin. (e) (6 pts) Indicate the charges of the four tryptic fragments at pH 8. ...
... (d) (5 pts) Indicate the positions of chymotrypsin cleavage, CNBr cleavage, and cleavage of the reduced and modified peptides with trypsin. (e) (6 pts) Indicate the charges of the four tryptic fragments at pH 8. ...
Amino acid profile of organically grown alternative agricultural
... artichoke originated in the American continent and has been first, a source for human food, and secondly, a source of animal food (Ben Chekroun et al., 1996). The main component of tubers’ dry matter content are carbohydrates and the greatest part of these consist of water soluble inulin (concentrat ...
... artichoke originated in the American continent and has been first, a source for human food, and secondly, a source of animal food (Ben Chekroun et al., 1996). The main component of tubers’ dry matter content are carbohydrates and the greatest part of these consist of water soluble inulin (concentrat ...
Are Hydrophobins and/or Non-Specific Lipid Transfer Proteins
... residues. These modifications must be accompanied with a shift of the isoelectric point from basic to acidic. One can imagine, that depending on mode and degree of modification quite a number of LTP1-species could be generated with molecular masses among 10 kDa and multiple acidic isolectric points. ...
... residues. These modifications must be accompanied with a shift of the isoelectric point from basic to acidic. One can imagine, that depending on mode and degree of modification quite a number of LTP1-species could be generated with molecular masses among 10 kDa and multiple acidic isolectric points. ...
Handout 14, 15 - U of L Class Index
... This is due to the extensive interaction between the two, covering 25nm2 of the surface area and involving the acceptor arm and the anticodon loop of the tRNA, as well as individual nucleotides in the D and the TΨC arm The interaction between enzyme and aa is less extensive, the aa is smaller, sever ...
... This is due to the extensive interaction between the two, covering 25nm2 of the surface area and involving the acceptor arm and the anticodon loop of the tRNA, as well as individual nucleotides in the D and the TΨC arm The interaction between enzyme and aa is less extensive, the aa is smaller, sever ...
Crystal structure of yeast hexokinase Pl in complex
... Hexokinase catalyses the formation of hexose-6-phosphate (mainly glucose-6phosphate, Glc-6P) from hexose using ATP as a phosphoryl donor. The isoenzymes differ in their activity toward particular hexoses. The enzymatic activity of PI isoenzyme with fructose and mannose is 2.6 and 0.6 times than that ...
... Hexokinase catalyses the formation of hexose-6-phosphate (mainly glucose-6phosphate, Glc-6P) from hexose using ATP as a phosphoryl donor. The isoenzymes differ in their activity toward particular hexoses. The enzymatic activity of PI isoenzyme with fructose and mannose is 2.6 and 0.6 times than that ...
Affinity Chromatography
... the partner as a template on which to model a biomimetic ligand (40); second, construction of a molecule which displays complementarity to exposed residues in the target site (41–44); and third, direct mimicking of natural biological recognition interactions (45). Peptidal templates comprising two o ...
... the partner as a template on which to model a biomimetic ligand (40); second, construction of a molecule which displays complementarity to exposed residues in the target site (41–44); and third, direct mimicking of natural biological recognition interactions (45). Peptidal templates comprising two o ...
APC5 Antibody
... to be essential for the normal progression of mitosis. APC5 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading ...
... to be essential for the normal progression of mitosis. APC5 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading ...