Identification of growth-dependent transcripts in the haloalkaliphilic
... subjected to further analysis. cDNA 1500 (~450 bp) was an ortholog of the histidine triad protein (HIT) superfamily (Hint branch) (Nmag_2944, E=7e-46) and cDNA 6350 (300 bp) was an ortholog of extracellular solutebinding protein family 5 of the ABC type peptide transporters (ABCt) (Nmag_4050, E=2e-5 ...
... subjected to further analysis. cDNA 1500 (~450 bp) was an ortholog of the histidine triad protein (HIT) superfamily (Hint branch) (Nmag_2944, E=7e-46) and cDNA 6350 (300 bp) was an ortholog of extracellular solutebinding protein family 5 of the ABC type peptide transporters (ABCt) (Nmag_4050, E=2e-5 ...
AP Biology The Biochemistry and Cell Signaling Pathway of the
... the dark coat-color phenotype. Based on your knowledge of the MC1R signaling pathway (Question 3), cell signaling, and the chemistry of the amino acid changes (Question 4), write a hypothesis for each of the following questions. a. How could the two extracellular mutations lead to the dark phenotype ...
... the dark coat-color phenotype. Based on your knowledge of the MC1R signaling pathway (Question 3), cell signaling, and the chemistry of the amino acid changes (Question 4), write a hypothesis for each of the following questions. a. How could the two extracellular mutations lead to the dark phenotype ...
G-protein - cloudfront.net
... Extends through the cell membrane. Intracellular part functions as a “kinase”, which transfers P from ATP to tyrosine on a substrate protein. ...
... Extends through the cell membrane. Intracellular part functions as a “kinase”, which transfers P from ATP to tyrosine on a substrate protein. ...
Chapter 11 PowerPoint 2016
... • Pathway similarities suggest that ancestral signaling molecules evolved in prokaryotes and were modified later in eukaryotes • The concentration of signaling molecules allows bacteria to detect population density ...
... • Pathway similarities suggest that ancestral signaling molecules evolved in prokaryotes and were modified later in eukaryotes • The concentration of signaling molecules allows bacteria to detect population density ...
Comparison with previous screens
... gene groups, such as those encoding kinesins, actin-binding proteins, kinases or phosphatases [6-9]. Our screen shares certain features with both the genome-wide and the gene-classspecific approaches. We performed RNAi experiments on a limited number of genes, but these genes include about one half ...
... gene groups, such as those encoding kinesins, actin-binding proteins, kinases or phosphatases [6-9]. Our screen shares certain features with both the genome-wide and the gene-classspecific approaches. We performed RNAi experiments on a limited number of genes, but these genes include about one half ...
shh
... – no-tail (ntl; fly brachyenteron), floating head (flh), momo (mom) have an absence of notochord • flh or mom: MFP present with associated LFP; LFP absent when MFP absent • In ntl: get expansion of MFP and LFP • LFP can develop without notochord (as can MFP), but LFP only in presence of MFP ...
... – no-tail (ntl; fly brachyenteron), floating head (flh), momo (mom) have an absence of notochord • flh or mom: MFP present with associated LFP; LFP absent when MFP absent • In ntl: get expansion of MFP and LFP • LFP can develop without notochord (as can MFP), but LFP only in presence of MFP ...
Gene Section GPC1 (glypican 1) Atlas of Genetics and Cytogenetics
... system (CNS) and skeletal system during development but also in many other tissues in the adult. ...
... system (CNS) and skeletal system during development but also in many other tissues in the adult. ...
Gene Section DDIT4 (DNA-damage-inducible transcript 4) Atlas of Genetics and Cytogenetics
... siRNA experiments in clear cell renal cell carcinoma (ccRCC) cell lines 786-O, A498 and Caki-2 (Kucejova et al., 2011). MEFs deficient for ataxia telangiectasia mutated (ATM) are unable to induce HIF1α (and REDD1) in response to hypoxia suggesting that, at least in some contexts, ATM may be required ...
... siRNA experiments in clear cell renal cell carcinoma (ccRCC) cell lines 786-O, A498 and Caki-2 (Kucejova et al., 2011). MEFs deficient for ataxia telangiectasia mutated (ATM) are unable to induce HIF1α (and REDD1) in response to hypoxia suggesting that, at least in some contexts, ATM may be required ...
Connecting oxidative stress, auxin, and cell cycle regulation through
... results). Upon activation of a MAPK pathway, MAPKs often induce expression of specific sets of genes. This expression mostly occurs through translocation of the active MAPK to the nucleus where the MAPK phosphorylates and thereby regulates the activity of specific transcription factors. However, MAP ...
... results). Upon activation of a MAPK pathway, MAPKs often induce expression of specific sets of genes. This expression mostly occurs through translocation of the active MAPK to the nucleus where the MAPK phosphorylates and thereby regulates the activity of specific transcription factors. However, MAP ...
regulation of eukaryotic gene expression
... • Specific transcription factors bind to enhancer regions or, in a few cases, to silencers and modulate the formation of the initiation complex, thus regulating the rate of initiation of transcription. • Each gene contains a variety of enhancer or silencer sequences in its regulatory region. The exa ...
... • Specific transcription factors bind to enhancer regions or, in a few cases, to silencers and modulate the formation of the initiation complex, thus regulating the rate of initiation of transcription. • Each gene contains a variety of enhancer or silencer sequences in its regulatory region. The exa ...
No Slide Title
... (Nüsslein-Volhard and Wieschaus): Idea is to find all possible genes that when ko’d could lead to a developmental anomaly… Approach: randomly mutagenize whole genome; Isolate single gene mutants; Study their phenotypes (in embryo) 40.000 individual mutations created: 150 very clear segmentation phen ...
... (Nüsslein-Volhard and Wieschaus): Idea is to find all possible genes that when ko’d could lead to a developmental anomaly… Approach: randomly mutagenize whole genome; Isolate single gene mutants; Study their phenotypes (in embryo) 40.000 individual mutations created: 150 very clear segmentation phen ...
Gene7-26
... proteins are associated with the plasma membrane. When bound by GDP the trimer remains intact and is inert. When the GDP is replaced by GTP, the a subunit is released from the bg dimer. Either the a monomer or the bg dimer then activates or represses a target protein. Monomeric G proteins are cytoso ...
... proteins are associated with the plasma membrane. When bound by GDP the trimer remains intact and is inert. When the GDP is replaced by GTP, the a subunit is released from the bg dimer. Either the a monomer or the bg dimer then activates or represses a target protein. Monomeric G proteins are cytoso ...
Supplementary Information (doc 5790K)
... cultured at different densities. (C) Confocal microscopy of YAP in MDA-MB-231 cells cultured in low (Low) or high (High) cell density. Scale bar, 20 μm. (D) Immunoblot analysis of YAP protein stability in cyclohexamide-treated MDAMB-231 ...
... cultured at different densities. (C) Confocal microscopy of YAP in MDA-MB-231 cells cultured in low (Low) or high (High) cell density. Scale bar, 20 μm. (D) Immunoblot analysis of YAP protein stability in cyclohexamide-treated MDAMB-231 ...
10456_2015_9485_MOESM2_ESM
... Supplementary Table 1. Genes up- or down-regulated 2 times or more by the depletion of EZH2 in HUVEC. Supplementary Table 2. Biological Process Gene Ontology terms that were significantly overrepresented in the analysis of the list of genes upregulated 2 times or more by EZHdepletion. Supplementary ...
... Supplementary Table 1. Genes up- or down-regulated 2 times or more by the depletion of EZH2 in HUVEC. Supplementary Table 2. Biological Process Gene Ontology terms that were significantly overrepresented in the analysis of the list of genes upregulated 2 times or more by EZHdepletion. Supplementary ...
Sp100 is important for the stimulatory effect of
... To investigate the impact of Sp100 on HIPK2-induced p53 activity, human U2OS cells were transfected with various combinations of expression vectors encoding Sp100 and the wild-type and kinase inactive (K221A) point mutant of HIPK2, along with a pG13-luc luciferase reporter gene controlled by multime ...
... To investigate the impact of Sp100 on HIPK2-induced p53 activity, human U2OS cells were transfected with various combinations of expression vectors encoding Sp100 and the wild-type and kinase inactive (K221A) point mutant of HIPK2, along with a pG13-luc luciferase reporter gene controlled by multime ...
- IMSA Digital Commons
... responsible for the dominating presence of glial cells in the adult brain remains well supported. ...
... responsible for the dominating presence of glial cells in the adult brain remains well supported. ...
MoFlo Fluorescence Resonance Energy Transfer E T
... An increase in the intensity of cells on the Y axis indicates FRET between Y-AID and C-ARH (lower plots), but not between Y-AID and C-ARHΔPTB (upper plots). Cells in the upper right quadrant in plots in Figure 2b were scored as being positive for FRET, and percentages in parentheses are the fraction ...
... An increase in the intensity of cells on the Y axis indicates FRET between Y-AID and C-ARH (lower plots), but not between Y-AID and C-ARHΔPTB (upper plots). Cells in the upper right quadrant in plots in Figure 2b were scored as being positive for FRET, and percentages in parentheses are the fraction ...
1984 BS, Seoul National University, Korea
... The N-end rule pathway is a proteolytic system in which destabilizing N-terminal residues of short-lived proteins function as a class of degradation signals (degrons), called N-degrons. Ndegrons are recognized by specific recognition components, such as ubiquitin ligases which mediate ubiquitination ...
... The N-end rule pathway is a proteolytic system in which destabilizing N-terminal residues of short-lived proteins function as a class of degradation signals (degrons), called N-degrons. Ndegrons are recognized by specific recognition components, such as ubiquitin ligases which mediate ubiquitination ...
PPT
... Chemical signals are converted from one type of signal into another to elicit a molecular response from the organism. All organisms require signaling pathways to live. ...
... Chemical signals are converted from one type of signal into another to elicit a molecular response from the organism. All organisms require signaling pathways to live. ...
Direct contributions of Otx2 as a positional tag to global gene
... for the formation of the eggshell, a complex structure that protects the embryo and mediates its interaction with the environment. Genetic studies of Drosophila oogenesis established it as a premiere model for the mechanistic analysis of developmental signaling [1]. Based on the results of these stu ...
... for the formation of the eggshell, a complex structure that protects the embryo and mediates its interaction with the environment. Genetic studies of Drosophila oogenesis established it as a premiere model for the mechanistic analysis of developmental signaling [1]. Based on the results of these stu ...
Cell Communication
... Pathway branching and “cross-talk” further help cell coordinate incoming ...
... Pathway branching and “cross-talk” further help cell coordinate incoming ...
Unit 3. Basic of Biopolymers (3) Control of Protein Function
... targeted to cellular compartments by signal sequences or by attachment of a lipid tail that inserts into membranes. directed to a complex of interacting proteins by a structural interaction domain Localization is a dynamic process and a given protein may be targeted to different compartments at diff ...
... targeted to cellular compartments by signal sequences or by attachment of a lipid tail that inserts into membranes. directed to a complex of interacting proteins by a structural interaction domain Localization is a dynamic process and a given protein may be targeted to different compartments at diff ...
cell communication powerpoint
... molecular interactions relay signals from receptors to target molecules in the cell • usually involves multiple steps – Benefits: • amplify a signal: A few molecules can produce a large cellular response • more opportunities for coordination and regulation of the cellular response ...
... molecular interactions relay signals from receptors to target molecules in the cell • usually involves multiple steps – Benefits: • amplify a signal: A few molecules can produce a large cellular response • more opportunities for coordination and regulation of the cellular response ...
MCDB 4650 Class 19 Patterning of the Limb
... What might induce the expression of Shh only in the posterior region of each limb bud ? a. Hox gene expression b. FGF signaling c. BMP signaling d. Wnt signaling ...
... What might induce the expression of Shh only in the posterior region of each limb bud ? a. Hox gene expression b. FGF signaling c. BMP signaling d. Wnt signaling ...
Hedgehog signaling pathway
The Hedgehog signaling pathway (or signalling pathway; see spelling differences) is a signaling pathway that transmits information to embryonic cells required for proper development. Different parts of the embryo have different concentrations of hedgehog signaling proteins. The pathway also has roles in the adult. Diseases associated with the malfunction of this pathway include basal cell carcinoma.The Hedgehog signaling pathway is one of the key regulators of animal development and is present in all bilaterians. The pathway takes its name from its polypeptide ligand, an intercellular signaling molecule called Hedgehog (Hh) found in fruit flies of the genus Drosophila. Hh is one of Drosophila's segment polarity gene products, involved in establishing the basis of the fly body plan. The molecule remains important during later stages of embryogenesis and metamorphosis.Mammals have three Hedgehog homologues, DHH, IHH, and SHH, of which Sonic (SHH) is the best studied. The pathway is equally important during vertebrate embryonic development. In knockout mice lacking components of the pathway, the brain, skeleton, musculature, gastrointestinal tract and lungs fail to develop correctly. Recent studies point to the role of Hedgehog signaling in regulating adult stem cells involved in maintenance and regeneration of adult tissues. The pathway has also been implicated in the development of some cancers. Drugs that specifically target Hedgehog signaling to fight this disease are being actively developed by a number of pharmaceutical companies.