48x36 Poster Template
... Atherosclerosis is associated with the buildup of plaques in the vascular walls. Currently, treatment for atherosclerosis involves preventative measures and surgical removal of plaque, angioplasty, and stent placement. We sought to develop an E. coli chassis delivering anti-atherosclerotic substance ...
... Atherosclerosis is associated with the buildup of plaques in the vascular walls. Currently, treatment for atherosclerosis involves preventative measures and surgical removal of plaque, angioplasty, and stent placement. We sought to develop an E. coli chassis delivering anti-atherosclerotic substance ...
liquid association-1..
... functionally associated genes are indeed uncorrelated • Microarray is too noisy •Biology is complex ...
... functionally associated genes are indeed uncorrelated • Microarray is too noisy •Biology is complex ...
Cell Signaling: A Molecular View
... Serotonin (blue) In all these figures the membrane is schematically shown in gray ...
... Serotonin (blue) In all these figures the membrane is schematically shown in gray ...
receptor
... signal finally triggers a specific cellular response. The response may be almost any imaginable cellular activity- such as catalysis by an enzyme, rearrangement of the cytoskeleton, or activation of specific genes in the nucleus. The cell signaling process helps ensure that crucial activities like t ...
... signal finally triggers a specific cellular response. The response may be almost any imaginable cellular activity- such as catalysis by an enzyme, rearrangement of the cytoskeleton, or activation of specific genes in the nucleus. The cell signaling process helps ensure that crucial activities like t ...
Chemical biology beyond binary codes
... while differing signi¢cantly in size, organization and functionality in the remaining portions of the two macrocycles. ...
... while differing signi¢cantly in size, organization and functionality in the remaining portions of the two macrocycles. ...
Protein Labeling
... • Receptor moieties must be amenable to genetic encoding as fusions to the protein of interest. • The receptors must be relatively small so as to not perturb protein function — an ideal receptor would be a short peptide sequence that could be inserted into various locations within the protein. • Che ...
... • Receptor moieties must be amenable to genetic encoding as fusions to the protein of interest. • The receptors must be relatively small so as to not perturb protein function — an ideal receptor would be a short peptide sequence that could be inserted into various locations within the protein. • Che ...
Anti-AP2M1 monoclonal antibody, clone 2D23
... transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 mu subunit binds to transmembrane cargo proteins; it recognizes the Y-X-X-Phi motifs. The surface region interacting with to the Y-X-X- ...
... transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 mu subunit binds to transmembrane cargo proteins; it recognizes the Y-X-X-Phi motifs. The surface region interacting with to the Y-X-X- ...
How and Why Does a Fly Turn Its Immune System Off?
... of PLoS Biology [19] builds on their past work showing that JNK signaling inhibits the imd pathway. The authors suggest that another extracellular signaling pathway, the JAK/STAT pathway, also inhibits imd signaling. Using cultured cells, they showed that the transcription factors Jra and Stat93E, w ...
... of PLoS Biology [19] builds on their past work showing that JNK signaling inhibits the imd pathway. The authors suggest that another extracellular signaling pathway, the JAK/STAT pathway, also inhibits imd signaling. Using cultured cells, they showed that the transcription factors Jra and Stat93E, w ...
Quality Control
... transfected with either Q25-GFP or Q103-GFP, as indicated, and sorted into populations containing the lowest or highest 10% of GFP fluorescence. Each lane contains lysates from ~40,000 cells. (B) Two-parameter FACS profiles of HEK cells transfected with GFP, Q25GFP, or Q103-GFP. GFP fluorescence is ...
... transfected with either Q25-GFP or Q103-GFP, as indicated, and sorted into populations containing the lowest or highest 10% of GFP fluorescence. Each lane contains lysates from ~40,000 cells. (B) Two-parameter FACS profiles of HEK cells transfected with GFP, Q25GFP, or Q103-GFP. GFP fluorescence is ...
邵吉民_Signaling_and_diseases
... down-regulation: decrease in number of receptors desensitization: decreased response to ligand stimulation up regulation: increase in number of receptors hypersensitivity: increased response to ligand stimulation, or self-activation without ligands ...
... down-regulation: decrease in number of receptors desensitization: decreased response to ligand stimulation up regulation: increase in number of receptors hypersensitivity: increased response to ligand stimulation, or self-activation without ligands ...
G-protein linked receptor
... Membranes continuous from cell to cell ER continuous from cell to cell thru “desmotubule” Limited to small molecules (<800 Da), but can open to let through 20,000 Da Primarily (but not exclusively) formed during cell division ...
... Membranes continuous from cell to cell ER continuous from cell to cell thru “desmotubule” Limited to small molecules (<800 Da), but can open to let through 20,000 Da Primarily (but not exclusively) formed during cell division ...
Presentation - people.vcu.edu
... binding to Frizzled (Kohn and Moon, 2005; Jenny and Mlodzik 2006) • Wnt will bind to Fz extracellularly, which will change Fz affinity to Dsh and cause it to interact intracellularly. • Dsh goes on to activate JNK and other proteins in order to affect cell movement through actin filament rearrangeme ...
... binding to Frizzled (Kohn and Moon, 2005; Jenny and Mlodzik 2006) • Wnt will bind to Fz extracellularly, which will change Fz affinity to Dsh and cause it to interact intracellularly. • Dsh goes on to activate JNK and other proteins in order to affect cell movement through actin filament rearrangeme ...
Case Study: Can A549 Cells Maintain Functionality and Signaling in
... secure a large inventory of a satisfactory lot for future use. A549 cells are derived from adenocarcinoma human alveolar basal epithelial cells. A549 cells are used as a model to study human cell biology, including signaling pathways. It is common to transfect reporter constructs into cell lines suc ...
... secure a large inventory of a satisfactory lot for future use. A549 cells are derived from adenocarcinoma human alveolar basal epithelial cells. A549 cells are used as a model to study human cell biology, including signaling pathways. It is common to transfect reporter constructs into cell lines suc ...
Document
... • Part of the GPCR C-terminus is tethered to the membrane by palmitoylation through a thioester linkage to a conserved Cys. • The G protein subunit has myristate attached via an amide linkage to an N-terminal Gly to effect membrane association. • The G protein subunit is incorporated into the me ...
... • Part of the GPCR C-terminus is tethered to the membrane by palmitoylation through a thioester linkage to a conserved Cys. • The G protein subunit has myristate attached via an amide linkage to an N-terminal Gly to effect membrane association. • The G protein subunit is incorporated into the me ...
Bacteria on target
... When ferric citrate binds, FecA activates periplasmic FecR, which then activates the sigma factor FecI, resulting in gene expression ...
... When ferric citrate binds, FecA activates periplasmic FecR, which then activates the sigma factor FecI, resulting in gene expression ...
View Full PDF - Biochemical Society Transactions
... the Snf1 kinase complex is regulated by the Rgt1–Glc7 phosphatase complex and by Hxk2. In the absence of any one of these proteins a conformational change is produced in the Snf1 complex that activates its protein kinase function. Thus, when Snf1 is activated by mutations in the HXK2 gene, it was ex ...
... the Snf1 kinase complex is regulated by the Rgt1–Glc7 phosphatase complex and by Hxk2. In the absence of any one of these proteins a conformational change is produced in the Snf1 complex that activates its protein kinase function. Thus, when Snf1 is activated by mutations in the HXK2 gene, it was ex ...
Definition of a RACK1 Interaction Network in Drosophila
... Identification of 37 RACK1 interacting proteins using Data-dependent acquisition In order to define the RACK1 interactome in Drosophila melanogaster, N- or C-terminal FLAG-tagged RACK1 were transiently expressed in Drosophila S2 cells, in mock or virus-infected conditions (Figure 1). A vector expres ...
... Identification of 37 RACK1 interacting proteins using Data-dependent acquisition In order to define the RACK1 interactome in Drosophila melanogaster, N- or C-terminal FLAG-tagged RACK1 were transiently expressed in Drosophila S2 cells, in mock or virus-infected conditions (Figure 1). A vector expres ...
“ Signal Transduction”?
... (GIST). However, scientists found no mutation of Abl kinase in these tumor cells. Please explain the underlying mechanism of how Gleevec is still working in this kind of cancer cells even without c-Abl mutation. ...
... (GIST). However, scientists found no mutation of Abl kinase in these tumor cells. Please explain the underlying mechanism of how Gleevec is still working in this kind of cancer cells even without c-Abl mutation. ...
Clean Solutions Fuel Affinity Chromatography
... -bacterial systems such as yeast, insect, mammalian or plant cells are also used3. Purification results from the specific interaction between two reaction partners. One reaction partner is the protein tag, the other is a ligand or antibody covalently bound to a matrix. The recombinant protein (or th ...
... -bacterial systems such as yeast, insect, mammalian or plant cells are also used3. Purification results from the specific interaction between two reaction partners. One reaction partner is the protein tag, the other is a ligand or antibody covalently bound to a matrix. The recombinant protein (or th ...
Plant growth: the translational connection
... machinery to regulate cell proliferation in unicellular organisms and body growth in multicellular organisms [5–7]. TOR responds to various inputs linked to the energy available to the cell such as amino acids, ATP status and stresses, but also to mitosis-promoting hormones (insulin) in multicellula ...
... machinery to regulate cell proliferation in unicellular organisms and body growth in multicellular organisms [5–7]. TOR responds to various inputs linked to the energy available to the cell such as amino acids, ATP status and stresses, but also to mitosis-promoting hormones (insulin) in multicellula ...
cover_article_1135_en_US
... via Ca2+/calmodulin (CaM)/signaling to human vacuolar protein sorting 34 (hVps34) due to AKT inhibition. DC120 also attenuated the inhibitory effect of AKT on C-Raf by decreasing the phosphorylation of C-Raf at Ser259 and activated the mitogen-activated protein kinase (MAPK) pathway. The activation ...
... via Ca2+/calmodulin (CaM)/signaling to human vacuolar protein sorting 34 (hVps34) due to AKT inhibition. DC120 also attenuated the inhibitory effect of AKT on C-Raf by decreasing the phosphorylation of C-Raf at Ser259 and activated the mitogen-activated protein kinase (MAPK) pathway. The activation ...
Slide 1
... subunits that together form the functional nAChR are shown. Note that each of the nAChR subunits wraps back and forth through the membrane four times and that the mature receptor is composed of five subunits. (B) Gprotein coupled receptors bind transmitter and, through a series of conformational cha ...
... subunits that together form the functional nAChR are shown. Note that each of the nAChR subunits wraps back and forth through the membrane four times and that the mature receptor is composed of five subunits. (B) Gprotein coupled receptors bind transmitter and, through a series of conformational cha ...
Lecture 14 Student Powerpoint
... Eukaryotes Previously we discussed aspects of transcription that involved the production of a primary transcript and the processing of this into aspects of how this is processed into an mRNA in Eukaryotes. This lecture focuses on these topics and on how signals are perceived that modulate the expres ...
... Eukaryotes Previously we discussed aspects of transcription that involved the production of a primary transcript and the processing of this into aspects of how this is processed into an mRNA in Eukaryotes. This lecture focuses on these topics and on how signals are perceived that modulate the expres ...
Hedgehog signaling pathway
The Hedgehog signaling pathway (or signalling pathway; see spelling differences) is a signaling pathway that transmits information to embryonic cells required for proper development. Different parts of the embryo have different concentrations of hedgehog signaling proteins. The pathway also has roles in the adult. Diseases associated with the malfunction of this pathway include basal cell carcinoma.The Hedgehog signaling pathway is one of the key regulators of animal development and is present in all bilaterians. The pathway takes its name from its polypeptide ligand, an intercellular signaling molecule called Hedgehog (Hh) found in fruit flies of the genus Drosophila. Hh is one of Drosophila's segment polarity gene products, involved in establishing the basis of the fly body plan. The molecule remains important during later stages of embryogenesis and metamorphosis.Mammals have three Hedgehog homologues, DHH, IHH, and SHH, of which Sonic (SHH) is the best studied. The pathway is equally important during vertebrate embryonic development. In knockout mice lacking components of the pathway, the brain, skeleton, musculature, gastrointestinal tract and lungs fail to develop correctly. Recent studies point to the role of Hedgehog signaling in regulating adult stem cells involved in maintenance and regeneration of adult tissues. The pathway has also been implicated in the development of some cancers. Drugs that specifically target Hedgehog signaling to fight this disease are being actively developed by a number of pharmaceutical companies.