The Guanine Nucleotide–Binding Switch in Three Dimensions
... between different membrane compartments in the cell. The inhibition of nucleotide release may be a rather accidental biochemical consequence of the interaction. Structures for RabGDI and RhoGDI have been solved, and they constitute two completely unrelated structural folds (3). The structures of the ...
... between different membrane compartments in the cell. The inhibition of nucleotide release may be a rather accidental biochemical consequence of the interaction. Structures for RabGDI and RhoGDI have been solved, and they constitute two completely unrelated structural folds (3). The structures of the ...
Searching for frameshift evolutionary relationships between protein
... least four representative domains were retained. This criterion was imposed in an effort to eliminate missequenced domains from our list. It is known that sequencing errors are quite common,10 and their presence among our list would lead to spurious frameshift relationships. By selecting only famili ...
... least four representative domains were retained. This criterion was imposed in an effort to eliminate missequenced domains from our list. It is known that sequencing errors are quite common,10 and their presence among our list would lead to spurious frameshift relationships. By selecting only famili ...
Amino acid sequence restriction in relation to proteolysis
... signals and sequence restrictions seem to apply to proteolysis, in a similar mode as earlier demonstrated for glycosylation. The pro-form cleavage may of course also be regulated by additional factors, and sequence restrictions may also apply to further types of proteolysis (especially, perhaps, to ...
... signals and sequence restrictions seem to apply to proteolysis, in a similar mode as earlier demonstrated for glycosylation. The pro-form cleavage may of course also be regulated by additional factors, and sequence restrictions may also apply to further types of proteolysis (especially, perhaps, to ...
search1
... • The first round of PSI-BLAST is a standard protein-protein BLAST search. The program builds a position-specific scoring matrix (PSSM or profile) from an alignment of the sequences returned with Expect values better (lower) than the inclusion threshold (default=0.005). • The PSSM will be used to ev ...
... • The first round of PSI-BLAST is a standard protein-protein BLAST search. The program builds a position-specific scoring matrix (PSSM or profile) from an alignment of the sequences returned with Expect values better (lower) than the inclusion threshold (default=0.005). • The PSSM will be used to ev ...
Evolutionary predictions of binding surfaces and interactions Olivier
... associated with specific functions and compiled, as, for example, in the INTERPRO database [13]. Global sequence homologies can also be identified by these methods or by the related and even more sensitive PSI-BLAST search [14,15], thereby suggesting putative functions and functional sites, by analo ...
... associated with specific functions and compiled, as, for example, in the INTERPRO database [13]. Global sequence homologies can also be identified by these methods or by the related and even more sensitive PSI-BLAST search [14,15], thereby suggesting putative functions and functional sites, by analo ...
A drug-controllable tag for visualizing newly synthesized proteins in
... tag whose presence on new proteins could be induced by the one-time administration of a small-molecule drug to either cells or animals. The strategy we devised was to incorporate a specific protease activity to confer self-removing behavior onto an epitope tag and then to use a corresponding proteas ...
... tag whose presence on new proteins could be induced by the one-time administration of a small-molecule drug to either cells or animals. The strategy we devised was to incorporate a specific protease activity to confer self-removing behavior onto an epitope tag and then to use a corresponding proteas ...
DV axis HT12
... oocyte, then to the cortex overlying the oocyte nucleus, and anchored there. Grk protein is synthesised, has limited diffusion, and signals to the follicle cells migrating overhead, which take on dorsal fates. ...
... oocyte, then to the cortex overlying the oocyte nucleus, and anchored there. Grk protein is synthesised, has limited diffusion, and signals to the follicle cells migrating overhead, which take on dorsal fates. ...
Supplementary Figure Captions (doc 46K)
... brightness (G,H) of the green (E,G) and red (F,H) liposomes in these incubations, as estimated using FIDA, is also shown (mean ± s.e.m., n = 10). These data show that whereas chloroform, as expected, causes liposome fusion (as indicated by a finite crosscorrelation signal, accompanied by a decrease ...
... brightness (G,H) of the green (E,G) and red (F,H) liposomes in these incubations, as estimated using FIDA, is also shown (mean ± s.e.m., n = 10). These data show that whereas chloroform, as expected, causes liposome fusion (as indicated by a finite crosscorrelation signal, accompanied by a decrease ...
Planta
... VvCTr1 in a yeast strain lacking all Ctr transporters fully rescued the phenotype, while a deficient complementation was observed in a strain lacking only plasma membrane-bound Ctrs. Given the common subcellular localization of VvCTr1 and AtCOPT5 and the highest amino acid sequence similarity in com ...
... VvCTr1 in a yeast strain lacking all Ctr transporters fully rescued the phenotype, while a deficient complementation was observed in a strain lacking only plasma membrane-bound Ctrs. Given the common subcellular localization of VvCTr1 and AtCOPT5 and the highest amino acid sequence similarity in com ...
Protein Crystallization
... Success rate of crystallization may be dramatically increased by the use of alternative reservoirs and additives, rather that expanding the range of conditions ...
... Success rate of crystallization may be dramatically increased by the use of alternative reservoirs and additives, rather that expanding the range of conditions ...
Protein prediction methods – steps of analysis
... classified upon their relationships to phospholipid bilayers i.e., those that are soluble and exist in the cytoplasm, in the lumen of an organelle or in the extracellular environments and those that are membrane attached associated wit a lipid bilayer. They can be integral membrane proteins or they ...
... classified upon their relationships to phospholipid bilayers i.e., those that are soluble and exist in the cytoplasm, in the lumen of an organelle or in the extracellular environments and those that are membrane attached associated wit a lipid bilayer. They can be integral membrane proteins or they ...
Delivery of a Secreted Soluble Protein to the Vacuole via a
... For soluble proteins, it has been shown that the critical sorting event occurs in the TGN, and that secretion is a default destination, whereas transport to the vacuole requires positive sorting information. In the yeast Saccharomyces cerevisiae, the vacuolar membrane appears to be the default desti ...
... For soluble proteins, it has been shown that the critical sorting event occurs in the TGN, and that secretion is a default destination, whereas transport to the vacuole requires positive sorting information. In the yeast Saccharomyces cerevisiae, the vacuolar membrane appears to be the default desti ...
EVALUATION OF ANTIOXIDANT AND ANTI-ARTHRITIC ACTIVITIES OF SOME 2-ARYLAMINOTHIAZOLE DERIVATIVES
... control. Diclofenac at the concentration 100 µg/ml and 200 µg/ml was used as reference drug, which also showed significant inhibition of protein denaturation i.e. 55.25 % and 90.41 % respectively (*p<0.001). Thus, TTA50, TTA51, TTA54 and TTA55 showed significant anti arthritic activity as demonstrat ...
... control. Diclofenac at the concentration 100 µg/ml and 200 µg/ml was used as reference drug, which also showed significant inhibition of protein denaturation i.e. 55.25 % and 90.41 % respectively (*p<0.001). Thus, TTA50, TTA51, TTA54 and TTA55 showed significant anti arthritic activity as demonstrat ...
CDPKs – a kinase for every Ca signal?
... by phospholipids such as phosphatidylserine and phosphatidylinositol is synergistic. However, because these lipids are not considered to be signaling molecules, it is arguable whether they serve as regulators or as structural components of membraneassociated CDPKs. One possible scenario is that cert ...
... by phospholipids such as phosphatidylserine and phosphatidylinositol is synergistic. However, because these lipids are not considered to be signaling molecules, it is arguable whether they serve as regulators or as structural components of membraneassociated CDPKs. One possible scenario is that cert ...
Nutrient Uptake, Solute Transport
... location to another is known as transport. Plants exchange solutes and water with their environment and among their tissues and organs. Both local and longdistance transport processes in plants are controlled largely by cellular membranes. Active and passive membrane transports are distinguished. Me ...
... location to another is known as transport. Plants exchange solutes and water with their environment and among their tissues and organs. Both local and longdistance transport processes in plants are controlled largely by cellular membranes. Active and passive membrane transports are distinguished. Me ...
Atom depth in protein structure and function
... water shell can be effectively represented in a crystal. It can be argued that hydrogen/deuterium (H/D) exchange rates, as measured in solution by nuclear magnetic resonance (NMR) [9], can provide a reliable test; however, there are also serious limitations. Common NMR techniques can directly monito ...
... water shell can be effectively represented in a crystal. It can be argued that hydrogen/deuterium (H/D) exchange rates, as measured in solution by nuclear magnetic resonance (NMR) [9], can provide a reliable test; however, there are also serious limitations. Common NMR techniques can directly monito ...
Regulation of cellular homoeostasis by reversible lysine acetylation
... activity in yeast, where the reduction in calories caused an increase in available NAD+, which is required for sirtuin function [11]. The ability of SIRT1 homologues to increase lifespan in various organisms led to the search for novel chemical activators. One of the best known is resveratrol, an an ...
... activity in yeast, where the reduction in calories caused an increase in available NAD+, which is required for sirtuin function [11]. The ability of SIRT1 homologues to increase lifespan in various organisms led to the search for novel chemical activators. One of the best known is resveratrol, an an ...
5-Cell and Molecular Biology (Golgi etc)
... These oligosaccharide processing pathways occur in a correspondingly organized sequence in the Golgi stack, with each cisterna containing its own set of processing enzymes Proteins are modified in successive stages as they move from cisterna to cisterna across the stack So that the stack forms ...
... These oligosaccharide processing pathways occur in a correspondingly organized sequence in the Golgi stack, with each cisterna containing its own set of processing enzymes Proteins are modified in successive stages as they move from cisterna to cisterna across the stack So that the stack forms ...
Molecular changes associated with the setting up of secondary
... of the TDFs. Among these, seven correspond to new genes encoding putative regulatory proteins. Emphasis was laid upon two genes encoding, respectively, an AP2/ERF-like transcription factor (PtaERF1) and a RING finger protein (PtaRHE1); their differential expression was further confirmed by reverse nor ...
... of the TDFs. Among these, seven correspond to new genes encoding putative regulatory proteins. Emphasis was laid upon two genes encoding, respectively, an AP2/ERF-like transcription factor (PtaERF1) and a RING finger protein (PtaRHE1); their differential expression was further confirmed by reverse nor ...
Chaperone-assisted protein folding: the path to discovery from a
... kinetically trapped states during folding. We suggested that the confining environment of the chaperonin cage not only prevents aggregation but also can smooth rugged foldingenergy landscapes, allowing folding to occur within a biologically relevant time frame43. A chaperone relay for protein foldin ...
... kinetically trapped states during folding. We suggested that the confining environment of the chaperonin cage not only prevents aggregation but also can smooth rugged foldingenergy landscapes, allowing folding to occur within a biologically relevant time frame43. A chaperone relay for protein foldin ...
IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) e-ISSN: 2278-3008.
... Insulin is a peptide hormone, produced by beta cells of the pancreas, and is central to regulating carbohydrate and fat metabolism in the body. Insulin causes cells in the liver, skeletal muscles, and fat tissue to absorb glucose from the blood. In the liver and skeletal muscles, glucose is stored a ...
... Insulin is a peptide hormone, produced by beta cells of the pancreas, and is central to regulating carbohydrate and fat metabolism in the body. Insulin causes cells in the liver, skeletal muscles, and fat tissue to absorb glucose from the blood. In the liver and skeletal muscles, glucose is stored a ...
Insights into digestion and absorption of major nutrients in humans
... glycosidic bonds between the carbon of one sugar and a hydroxyl group on another sugar. The bond may be either ␣ or , depending on its position above or below the plane of the sugar. [Modified from Ref. 6.] ...
... glycosidic bonds between the carbon of one sugar and a hydroxyl group on another sugar. The bond may be either ␣ or , depending on its position above or below the plane of the sugar. [Modified from Ref. 6.] ...
Genetically Encoded Fluorescent Reporters of Histone
... in intact, living cells. Here, we describe a new class of fluorescent reporters that can be used in single, living cells to track histone methylation with high spatial and temporal resolution. The general reporter design is shown in Figure 1a. A peptide substrate corresponding to the methylation sit ...
... in intact, living cells. Here, we describe a new class of fluorescent reporters that can be used in single, living cells to track histone methylation with high spatial and temporal resolution. The general reporter design is shown in Figure 1a. A peptide substrate corresponding to the methylation sit ...
Exploration of the Dynamic Properties of Protein Complexes
... Protein complexes are not static, but rather highly dynamic with subunits that undergo 1-dimensional diffusion with respect to each other. Interactions within protein complexes are modulated through regulatory inputs that alter interactions and introduce new components and deplete existing component ...
... Protein complexes are not static, but rather highly dynamic with subunits that undergo 1-dimensional diffusion with respect to each other. Interactions within protein complexes are modulated through regulatory inputs that alter interactions and introduce new components and deplete existing component ...