BMC Microbiology
... the induction of the yeast to mycelium transition. The second calcium uptake peak was observed 300 min after induction, at the time of DNA synthesis. When different substances that affected calcium uptake were added to the medium during the yeast to mycelium transition such as cobalt ions, ionophore ...
... the induction of the yeast to mycelium transition. The second calcium uptake peak was observed 300 min after induction, at the time of DNA synthesis. When different substances that affected calcium uptake were added to the medium during the yeast to mycelium transition such as cobalt ions, ionophore ...
A Novel Nuclear Pore Protein Nup82p Which
... against nucleoporins as well as WGA were used to inhibit nuclear accumulation of proteins and RNA export reactions (Featherstone et al., 1988; Finlay et al., 1987). Immunodepletion of nuclear pore proteins such as p62 and its associated p58 and p54 components from Xenopus egg extracts used to recons ...
... against nucleoporins as well as WGA were used to inhibit nuclear accumulation of proteins and RNA export reactions (Featherstone et al., 1988; Finlay et al., 1987). Immunodepletion of nuclear pore proteins such as p62 and its associated p58 and p54 components from Xenopus egg extracts used to recons ...
Bchm2000_P1 - U of L Class Index
... the column. Thus, smaller solutes are retarded relative to larger solutes which do not enter the pores. (27) The unknown protein must be exposed to conditions that (1) disrupt quaternary structure and (2) maintain quaternary structure. SDS-PAGE in the presence of reducing agent (2-mercaptoethanol) w ...
... the column. Thus, smaller solutes are retarded relative to larger solutes which do not enter the pores. (27) The unknown protein must be exposed to conditions that (1) disrupt quaternary structure and (2) maintain quaternary structure. SDS-PAGE in the presence of reducing agent (2-mercaptoethanol) w ...
Protein Requirements of Pregnant and Lactating Women
... curves in that report showed that for countries such as India, during pregnancy – something that might be obscured by the the GWG was between 8 and 10 kg at term, for birth weights weight gain due to the fetus as well as to fluid gain and fat acranging from < 2500 to > 3000 g, in women whose mean he ...
... curves in that report showed that for countries such as India, during pregnancy – something that might be obscured by the the GWG was between 8 and 10 kg at term, for birth weights weight gain due to the fetus as well as to fluid gain and fat acranging from < 2500 to > 3000 g, in women whose mean he ...
Developmental Analysis of a Putative ATP/ADP Carrier Protein
... reached the maximum level at 5–6 d like the developmental pattern of PMP38, and then decrease after exposure to light. After the completion of the peroxisome transition, proteins of the fatty acid >-oxidation enzymes were still detectable in leaf peroxisomes like the developmental pattern of PMP38, ...
... reached the maximum level at 5–6 d like the developmental pattern of PMP38, and then decrease after exposure to light. After the completion of the peroxisome transition, proteins of the fatty acid >-oxidation enzymes were still detectable in leaf peroxisomes like the developmental pattern of PMP38, ...
A single amino acid substitution in the haemagglutinin
... (Lamb et al., 2006). Although the described interaction between the HN and F proteins is generally agreed upon, the relationships between receptor recognition, HN structure, the HN–F interaction and fusion are still poorly understood (Lamb & Parks, 2007; Lamb et al., 2006; Li et al., 2004; Morrison, ...
... (Lamb et al., 2006). Although the described interaction between the HN and F proteins is generally agreed upon, the relationships between receptor recognition, HN structure, the HN–F interaction and fusion are still poorly understood (Lamb & Parks, 2007; Lamb et al., 2006; Li et al., 2004; Morrison, ...
capitolo 1 - Structural Biology
... Main properties of the lateral chains We recall briefly some features of the amino acids. Amino acids can be distinguished into different classes in view of their polarità. By varying the criterion for classification it changes the class to which the amino acids belong. Tryptophan and arginine for ...
... Main properties of the lateral chains We recall briefly some features of the amino acids. Amino acids can be distinguished into different classes in view of their polarità. By varying the criterion for classification it changes the class to which the amino acids belong. Tryptophan and arginine for ...
Plant mitochondria contain the protein translocase subunits TatB
... that the At5g43680 and related proteins grouped most closely with the TatB proteins from bacteria indicating that At5g43680 is a possible TatB like protein (Figure 2). Although some of the bootstrap values are low we believe the tree is accurate. We also tested Bayesian homology using MrBayes (Ronq ...
... that the At5g43680 and related proteins grouped most closely with the TatB proteins from bacteria indicating that At5g43680 is a possible TatB like protein (Figure 2). Although some of the bootstrap values are low we believe the tree is accurate. We also tested Bayesian homology using MrBayes (Ronq ...
Algorithms and a Software Application for the Discovery of Heparin
... order to prevent this, proteins that easily bind to heparin need to be added to the solution. Then, the unwanted substances can easily be filtered out. Certain sequences or patterns of amino acids are known to have a high probability of binding to heparin. Thus, proteins that contain large numbers o ...
... order to prevent this, proteins that easily bind to heparin need to be added to the solution. Then, the unwanted substances can easily be filtered out. Certain sequences or patterns of amino acids are known to have a high probability of binding to heparin. Thus, proteins that contain large numbers o ...
association of drg1 and drg2 with ribosomes from pea, arabidopsis
... the archetypes for the DRG1 and DRG2 orthologous groups (Caldon et al. 2001; Leipe et al. 2002). All eukaryotes examined contain at least one member of each group (Li and Trueb 2000; Tatusov et al. 2003). Amino acid identity among plant, animal, and fungal representatives of each orthologous group i ...
... the archetypes for the DRG1 and DRG2 orthologous groups (Caldon et al. 2001; Leipe et al. 2002). All eukaryotes examined contain at least one member of each group (Li and Trueb 2000; Tatusov et al. 2003). Amino acid identity among plant, animal, and fungal representatives of each orthologous group i ...
The role of lipids in the biogenesis of integral membrane
... full transporter function of LacY in cells lacking PE correlates with a structural alteration in the periplasmic domain P7 of LacY, as indicated by a loss of recognition by a conformation-sensitive monoclonal antibody (Fig. 1). PE is not required for membrane insertion of LacY, but it is required to ...
... full transporter function of LacY in cells lacking PE correlates with a structural alteration in the periplasmic domain P7 of LacY, as indicated by a loss of recognition by a conformation-sensitive monoclonal antibody (Fig. 1). PE is not required for membrane insertion of LacY, but it is required to ...
ppt - Chair of Computational Biology
... Several sequences in the GPCR dataset contained non-standard amino acid codes not present in the table of z-values. In such cases, the following substitutions were made. Where the sequence contained a ‘B’ (either an asparagine or aspartic acid) the residue was assigned as an asparagine ‘N’. Where th ...
... Several sequences in the GPCR dataset contained non-standard amino acid codes not present in the table of z-values. In such cases, the following substitutions were made. Where the sequence contained a ‘B’ (either an asparagine or aspartic acid) the residue was assigned as an asparagine ‘N’. Where th ...
ProtocolTargetDiscoveryVDS_Spring13
... Does the enzyme work in a complex of other proteins/macromolecules or does it do its job alone? ESSENTIALITY INFO Ideally we want an enzyme that is crucial for the survival or virulence of the organism. Some of the databases have information on this however, it may be necessary to read into the jour ...
... Does the enzyme work in a complex of other proteins/macromolecules or does it do its job alone? ESSENTIALITY INFO Ideally we want an enzyme that is crucial for the survival or virulence of the organism. Some of the databases have information on this however, it may be necessary to read into the jour ...
Patrick Cramer Anton Meinhart, Tobias Silberzahn and
... Applied Science) incubation on ice for 20 min. The slurry was cleared by centrifugation, and the supernatant was loaded onto a nickel-nitrilotriacetic acid-agarose column (Qiagen). The column was washed with 20 column volumes (CV) of buffer A followed by 5 CV of buffer B (buffer A but with 300 mM Na ...
... Applied Science) incubation on ice for 20 min. The slurry was cleared by centrifugation, and the supernatant was loaded onto a nickel-nitrilotriacetic acid-agarose column (Qiagen). The column was washed with 20 column volumes (CV) of buffer A followed by 5 CV of buffer B (buffer A but with 300 mM Na ...
8. ARTÍCULOS
... binding sites and, ¢nally, the HECT domain, which has already been shown to bind ubiquitin in a dithiothreitol-sensitive manner, thus con¢rming its ability to act as an E3 ubiquitin-protein ligase [17]. All the above-stated conserved regions present in HERC1 have already been involved in the formati ...
... binding sites and, ¢nally, the HECT domain, which has already been shown to bind ubiquitin in a dithiothreitol-sensitive manner, thus con¢rming its ability to act as an E3 ubiquitin-protein ligase [17]. All the above-stated conserved regions present in HERC1 have already been involved in the formati ...
C. Flow Chart
... hypothesized to be in thermodynamic equilibrium with their environment. Thus the primary determinant of a protein structure is considered to be its atomic interactions, i.e. its amino acid sequence. An analogous conjecture has arisen at the local scale. The modeling of protein loops is often conside ...
... hypothesized to be in thermodynamic equilibrium with their environment. Thus the primary determinant of a protein structure is considered to be its atomic interactions, i.e. its amino acid sequence. An analogous conjecture has arisen at the local scale. The modeling of protein loops is often conside ...
Protein thermal stability: insights from atomic displacement
... the percentage Glu residues in high B value regions is nearly twice and that of Lys is nearly 1.5 times higher in thermophiles than mesophiles. In contrast, the percentages of Ser and Thr in high B value regions of thermophiles are decreased by half (Table IV). These are also related to larger p-val ...
... the percentage Glu residues in high B value regions is nearly twice and that of Lys is nearly 1.5 times higher in thermophiles than mesophiles. In contrast, the percentages of Ser and Thr in high B value regions of thermophiles are decreased by half (Table IV). These are also related to larger p-val ...
Lecture 06 Absorption 1
... The macrominerals for the most part rely on diffusion controlled mechanisms combined with specific channel proteins to pass into the system. Gradients across the membrane can be driven by unidirectional and bidirectional ATPase enzymes Example Na+/K+ ATPase ...
... The macrominerals for the most part rely on diffusion controlled mechanisms combined with specific channel proteins to pass into the system. Gradients across the membrane can be driven by unidirectional and bidirectional ATPase enzymes Example Na+/K+ ATPase ...
Sample pages 2 PDF
... amino group as shown in Fig. 2.1. The amide bonds are specifically called the peptide bonds. If two amino acids are condensed, the product is called as dipeptide. When another amino acid condenses to this dipeptide, a tripeptide is formed. In this manner, a chain of amino acids can be linked to make ...
... amino group as shown in Fig. 2.1. The amide bonds are specifically called the peptide bonds. If two amino acids are condensed, the product is called as dipeptide. When another amino acid condenses to this dipeptide, a tripeptide is formed. In this manner, a chain of amino acids can be linked to make ...
Gene regulation in physiological stress
... have become relatively simple tools that can be put to excellent use in comparative biochemistry. Gene discovery techniques such as cDNA array screening are providing amazing opportunities for identifying the genes that are turned on in animals under different environmental stresses, frequently high ...
... have become relatively simple tools that can be put to excellent use in comparative biochemistry. Gene discovery techniques such as cDNA array screening are providing amazing opportunities for identifying the genes that are turned on in animals under different environmental stresses, frequently high ...
Functional decorations: post-translational modifications and heart
... (O-GlcNAcylation) to serine or threonine residues of predominantly nucleocytoplasmic proteins. O-GlcNAc thus has the potential to compete with phosphorylation for binding sites [49]. This has led to the proposal of OGlcNAc-phosphate crosstalk (see below), although there are currently few examples of ...
... (O-GlcNAcylation) to serine or threonine residues of predominantly nucleocytoplasmic proteins. O-GlcNAc thus has the potential to compete with phosphorylation for binding sites [49]. This has led to the proposal of OGlcNAc-phosphate crosstalk (see below), although there are currently few examples of ...
BMC Bioinformatics
... Background: Type III secretion system (T3SS) is a specialized protein delivery system in gramnegative bacteria that injects proteins (called effectors) directly into the eukaryotic host cytosol and facilitates bacterial infection. For many plant and animal pathogens, T3SS is indispensable for diseas ...
... Background: Type III secretion system (T3SS) is a specialized protein delivery system in gramnegative bacteria that injects proteins (called effectors) directly into the eukaryotic host cytosol and facilitates bacterial infection. For many plant and animal pathogens, T3SS is indispensable for diseas ...
The dinitrogenase reductase
... allosteric inhibition by having Tyr397 residue modified by adenylylation. • The addition and removal of the AMP group to the glutamine synthetase are catalyzed by two active sites of the same bifunctional adenylyltransferase (AT). • The substrate specificity of AT was found to be controlled by a reg ...
... allosteric inhibition by having Tyr397 residue modified by adenylylation. • The addition and removal of the AMP group to the glutamine synthetase are catalyzed by two active sites of the same bifunctional adenylyltransferase (AT). • The substrate specificity of AT was found to be controlled by a reg ...
everything you need to know about protein - Bio
... this lost tissue as quickly as possible. One type of protein may offer specific benefits that another protein may not. Since your body uses the different attributes of proteins for a wide variety of functions, it is best to consume several types of protein each day. For this reason Bio-Synergy uses ...
... this lost tissue as quickly as possible. One type of protein may offer specific benefits that another protein may not. Since your body uses the different attributes of proteins for a wide variety of functions, it is best to consume several types of protein each day. For this reason Bio-Synergy uses ...