Figure 6 The RAD51 ATP-binding site
... pGAT3, a member of the pGAT series of expression vectors that allow production of the target gene fused to a double amino-terminal tag consisting of a six histidine sequence followed by the glutathioneS-transferase protein 35. The BRC4 - RAD51 fusion protein was overexpressed in E. coli strain BL21( ...
... pGAT3, a member of the pGAT series of expression vectors that allow production of the target gene fused to a double amino-terminal tag consisting of a six histidine sequence followed by the glutathioneS-transferase protein 35. The BRC4 - RAD51 fusion protein was overexpressed in E. coli strain BL21( ...
Different Types of Labs for Students Considering a Career in
... Personnel in mass spectrometry labs perform techniques in which radioactive isotopes are used to measure cell metabolism; e.g., the rate of protein synthesis or the metabolism of a specific chemical, such as glucose. ...
... Personnel in mass spectrometry labs perform techniques in which radioactive isotopes are used to measure cell metabolism; e.g., the rate of protein synthesis or the metabolism of a specific chemical, such as glucose. ...
Plasma Proteins - neutralposture
... 10% Cu bound with albumin loosely Lower level of ceruloplasmin in Wilson’s disease, malnutrition, nephrosis, cirrhosis Increased level in active hepatitis, biliary cirrhosis, hemochromatosis, obstructive ...
... 10% Cu bound with albumin loosely Lower level of ceruloplasmin in Wilson’s disease, malnutrition, nephrosis, cirrhosis Increased level in active hepatitis, biliary cirrhosis, hemochromatosis, obstructive ...
Quality Attributes of Biologics
... metabolism and is secreted by the ultimobranchial gland of salmon. It is produced from either synthetic processes or microbial processes using recombinant DNA (rDNA) technology. The host cell-derived protein content and the host cell- or vector-derived DNA content of Calcitonin Salmon produced from ...
... metabolism and is secreted by the ultimobranchial gland of salmon. It is produced from either synthetic processes or microbial processes using recombinant DNA (rDNA) technology. The host cell-derived protein content and the host cell- or vector-derived DNA content of Calcitonin Salmon produced from ...
(FADD) in a total cell lysate
... increase the intensity of signal, several parameters were optimized like the choice of the parent ion (tryptic peptide) and of the transition as well as the MRM parameters. A relatively good detection of FADD in total cell lysates was only possible after optimization. Now, the next step will be quan ...
... increase the intensity of signal, several parameters were optimized like the choice of the parent ion (tryptic peptide) and of the transition as well as the MRM parameters. A relatively good detection of FADD in total cell lysates was only possible after optimization. Now, the next step will be quan ...
Slide 1
... • display biological samples or each MS/MS samples • display sequence coverages, number of total or unique peptides assigned to each protein etc. “Export” you can export your results into excel spreadsheets • the spreadsheet will contain the report you have chosen and a short description of the ...
... • display biological samples or each MS/MS samples • display sequence coverages, number of total or unique peptides assigned to each protein etc. “Export” you can export your results into excel spreadsheets • the spreadsheet will contain the report you have chosen and a short description of the ...
In Depth Analysis of the Spectra Unassigned by Database Search
... Due to unexpected PTMs, mutations, contaminants and novel peptides, nearly every proteomics mass spectrometry (MS) experiment produces a large amount of high-quality spectra not matched by any database peptides. The confident identification of these "non-database" peptides are valuable for all prote ...
... Due to unexpected PTMs, mutations, contaminants and novel peptides, nearly every proteomics mass spectrometry (MS) experiment produces a large amount of high-quality spectra not matched by any database peptides. The confident identification of these "non-database" peptides are valuable for all prote ...
GenScript - Protein Services
... Note: An extra of 4-6 aa will be left after protease cleavage of C-terminal tag, therefore, we DO NOT recommend the introduction of protease cleavage site between the target protein and the C-terminal tag. Do you want GenScript to remove tag(s) in the purification step? Yes ...
... Note: An extra of 4-6 aa will be left after protease cleavage of C-terminal tag, therefore, we DO NOT recommend the introduction of protease cleavage site between the target protein and the C-terminal tag. Do you want GenScript to remove tag(s) in the purification step? Yes ...
Supplementary Methods and References
... PMSF and 1 x protease inhibitor cocktail [Sigma]). 500 μg protein extract was mixed with 25 μL GFP-Trap®-M magnetic beads (Chromotek), and incubated for 2h at 4°C with rotation. After collecting the beads on a magnetic platform, the supernatant was removed and beads were washed extensively three tim ...
... PMSF and 1 x protease inhibitor cocktail [Sigma]). 500 μg protein extract was mixed with 25 μL GFP-Trap®-M magnetic beads (Chromotek), and incubated for 2h at 4°C with rotation. After collecting the beads on a magnetic platform, the supernatant was removed and beads were washed extensively three tim ...
Purification, Identification and Characterisation of - DORAS
... physiological substrate has clearly been defined for this protease but its ability to effectively degrade gelatin suggests a candidate protein substrate in vivo and a possible role in extracellular matrix protein degradation. Key Words: Z-Pro-prolinal Insensitive Peptidase; prolyl oligopeptidase; se ...
... physiological substrate has clearly been defined for this protease but its ability to effectively degrade gelatin suggests a candidate protein substrate in vivo and a possible role in extracellular matrix protein degradation. Key Words: Z-Pro-prolinal Insensitive Peptidase; prolyl oligopeptidase; se ...
$doc.title
... developed and used to identify potent ABHD6 inhibitors. Matrixassisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS) was used to analyze hABHD6 by proteomic peptide fingerprinting and the identification covalent interactions between the active-site serine and potent inh ...
... developed and used to identify potent ABHD6 inhibitors. Matrixassisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS) was used to analyze hABHD6 by proteomic peptide fingerprinting and the identification covalent interactions between the active-site serine and potent inh ...
Top down - The Fenyo Lab
... RED: triplicate experiments, FAl treated grindate BLACK: duplicated experiments, FAl treated cells (then ground) SCORE: Log Ion Current / Log protein abundance ...
... RED: triplicate experiments, FAl treated grindate BLACK: duplicated experiments, FAl treated cells (then ground) SCORE: Log Ion Current / Log protein abundance ...
HIV protease cleaves poly(A)
... SV-PR-expressing cells when SQ was present (Figure 2A). Cell extracts were analysed further to determine the cleavage of eIF4GI and eIF4GII (Figures 2B and 2C). Previous findings revealed the presence of two forms of eIF4G of 220 and 150 kDa in BHK21 cells [27,28]. These different eIF4G forms may be ...
... SV-PR-expressing cells when SQ was present (Figure 2A). Cell extracts were analysed further to determine the cleavage of eIF4GI and eIF4GII (Figures 2B and 2C). Previous findings revealed the presence of two forms of eIF4G of 220 and 150 kDa in BHK21 cells [27,28]. These different eIF4G forms may be ...
Nutrition Label - Herbalwell.com
... Suggested Serving: Take one tablet with each protein- S u p p l e containing meal. Serving Size: 1 Tablet For Maximum Results: Use this product in conjunction with Servings Per Container: 60 other Herbalife products. ...
... Suggested Serving: Take one tablet with each protein- S u p p l e containing meal. Serving Size: 1 Tablet For Maximum Results: Use this product in conjunction with Servings Per Container: 60 other Herbalife products. ...
Design of specific peptide Inhibitors of Phospholipase A2
... Phospholipase A2 (E.C. 3.1.1.4) is a key enzyme of the cascade mechanism involved in the production of proinflammatory compounds known as eicosanoids [1]. Specific inhibitor design is therefore of highest interest [2,3]. The binding of phospholipase A2 to membrane surfaces and hydrolysis of phosphol ...
... Phospholipase A2 (E.C. 3.1.1.4) is a key enzyme of the cascade mechanism involved in the production of proinflammatory compounds known as eicosanoids [1]. Specific inhibitor design is therefore of highest interest [2,3]. The binding of phospholipase A2 to membrane surfaces and hydrolysis of phosphol ...
Answers
... CLUSTALW 1.8 was used for multiple alignment of the three sequences. Areas where there were difference between the sequences were identified using Boxshade version 3.21 – these are shown in the file “Boxshade results”. It can be see that the VEGA sequence has an extra 22 amino acids at the N terminu ...
... CLUSTALW 1.8 was used for multiple alignment of the three sequences. Areas where there were difference between the sequences were identified using Boxshade version 3.21 – these are shown in the file “Boxshade results”. It can be see that the VEGA sequence has an extra 22 amino acids at the N terminu ...
Accelerating Protein ID for Deep Proteome Profiling
... can be reflected in the score from the subsequent database search. Here, the score distribution (Figure 4, top) indicated that the majority of MS/MS spectra acquired were of high quality, score of 8 or more (blue). When the distribution for the MS/MS spectra that resulted in a confident peptide ID i ...
... can be reflected in the score from the subsequent database search. Here, the score distribution (Figure 4, top) indicated that the majority of MS/MS spectra acquired were of high quality, score of 8 or more (blue). When the distribution for the MS/MS spectra that resulted in a confident peptide ID i ...
Final Report SID5
... The bacterium Streptococcus uberis is a common cause of intramammary infection in dairy cattle and is a leading cause of bovine mastitis worldwide. In the UK it has recently been shown that S. uberis is the most common cause of clinical mastitis. The ability of the organism to grow in milk has been ...
... The bacterium Streptococcus uberis is a common cause of intramammary infection in dairy cattle and is a leading cause of bovine mastitis worldwide. In the UK it has recently been shown that S. uberis is the most common cause of clinical mastitis. The ability of the organism to grow in milk has been ...
Biological assays are gaining ground
... the technique that gave the most reproducible results. RNA-interference has developed into an extremely powerful tool for target identification by linking loss of function phenotypes to cellular pathways. Tschulena et al., who used dynamic impedance analysis of knock-downs, report in this issue (see ...
... the technique that gave the most reproducible results. RNA-interference has developed into an extremely powerful tool for target identification by linking loss of function phenotypes to cellular pathways. Tschulena et al., who used dynamic impedance analysis of knock-downs, report in this issue (see ...
The samples were dissolved in 25 µL of 100 mM ammonium
... then reducing with DTT at 90°C for 20 min. Alkylation was done using iodoacetamide (IAM) for 1 hour at room temperature followed by a quenching step with DTT to remove excess IAM. The enzyme samples were then diluted 10-fold with 25 mM ammonium bicarbonate and enzyme was added (1:50 enzyme:substrate ...
... then reducing with DTT at 90°C for 20 min. Alkylation was done using iodoacetamide (IAM) for 1 hour at room temperature followed by a quenching step with DTT to remove excess IAM. The enzyme samples were then diluted 10-fold with 25 mM ammonium bicarbonate and enzyme was added (1:50 enzyme:substrate ...
Solid Tumour Section Bone: t(1;17)(p34;p13) in aneurysmal bone cyst
... and domains with similarity with BCLAF1 and with CASC3/MLN51 in the C-terminal region. It is part of the transcription regulatory complex TRAP/Mediator, and a component of the spliceosome. It both activates pre-mRNA splicing and induces mRNA degradation. The arginine/serine-rich N-term of THRAP3 is ...
... and domains with similarity with BCLAF1 and with CASC3/MLN51 in the C-terminal region. It is part of the transcription regulatory complex TRAP/Mediator, and a component of the spliceosome. It both activates pre-mRNA splicing and induces mRNA degradation. The arginine/serine-rich N-term of THRAP3 is ...
SystemsBiologyPaper
... analyzed in the mass spectrometer [7]. There have been several methods created to use isotope labels to identify two protein populations in different states or at different time points [7, 9]. These methods allow for a more dynamic analysis of protein populations. One other technique that may gain p ...
... analyzed in the mass spectrometer [7]. There have been several methods created to use isotope labels to identify two protein populations in different states or at different time points [7, 9]. These methods allow for a more dynamic analysis of protein populations. One other technique that may gain p ...
ClgR regulation of chaperone and protease systems is essential for
... The genome of Mycobacterium tuberculosis encodes approximately 50 proteases, including two paralogues of ClpP and the associated ATPase chaperones ClpC and ClpX (RibeiroGuimarães & Pessolani, 2007). In addition, M. tuberculosis possesses FtsH and is one of a select group of bacteria that possess a ...
... The genome of Mycobacterium tuberculosis encodes approximately 50 proteases, including two paralogues of ClpP and the associated ATPase chaperones ClpC and ClpX (RibeiroGuimarães & Pessolani, 2007). In addition, M. tuberculosis possesses FtsH and is one of a select group of bacteria that possess a ...