The regulation of fatty acid biosynthesis in some
... discussed in the context of the suggestion made by Intriago and Floodgate (Journal of General Microbiology 137, 1503-1509,1991) that Ffexibucterstrains possess both the anaerobic and aerobic pathways for unsaturated fatty acid biosynthesis, the activity of the latter being modulated by the intracell ...
... discussed in the context of the suggestion made by Intriago and Floodgate (Journal of General Microbiology 137, 1503-1509,1991) that Ffexibucterstrains possess both the anaerobic and aerobic pathways for unsaturated fatty acid biosynthesis, the activity of the latter being modulated by the intracell ...
Exploring the Effects of Human Cytomegalovirus Infection on Host
... latently infect a majority of the world’s population. Though infections are usually minor, HCMV can cause serious problems in the immunocompromised and is a leading cause of birth defects. When HCMV infects a human cell, it effectively turns the cell into a “virus-producing factory”, taking control ...
... latently infect a majority of the world’s population. Though infections are usually minor, HCMV can cause serious problems in the immunocompromised and is a leading cause of birth defects. When HCMV infects a human cell, it effectively turns the cell into a “virus-producing factory”, taking control ...
Physiology of Saccharomyces cerevisiae in anaerobic glucose
... considerably higher than for CBS 8066,despite a similar cell composition. The major difference between the two yeast strains was that S.cerevisiae H1022 did not produce acetate, suggesting that the observed difference in cell yield may be ascribed to an uncoupling effect of acetic acid. The absence ...
... considerably higher than for CBS 8066,despite a similar cell composition. The major difference between the two yeast strains was that S.cerevisiae H1022 did not produce acetate, suggesting that the observed difference in cell yield may be ascribed to an uncoupling effect of acetic acid. The absence ...
Modification of halogen specificity of a vanadium‐dependent
... Schijndel et al. 1993), which was originally designated as CPO. Additional mutations should be performed to enhance CPO activity of the BPO from C. pilulifera. We have prepared another mutant enzyme, H480F; however, no enzymatic activity was detected for this protein (data not shown). It has been st ...
... Schijndel et al. 1993), which was originally designated as CPO. Additional mutations should be performed to enhance CPO activity of the BPO from C. pilulifera. We have prepared another mutant enzyme, H480F; however, no enzymatic activity was detected for this protein (data not shown). It has been st ...
biochemistry - Louis Bolk Instituut
... How can we do justice to life itself when studying the life sciences? Biochemistry is the area in the life sciences which pre-eminently offers insight into the continuous and manifold changes that occur in organisms. It shows substances to be not static but ever changing, in structure as well as fun ...
... How can we do justice to life itself when studying the life sciences? Biochemistry is the area in the life sciences which pre-eminently offers insight into the continuous and manifold changes that occur in organisms. It shows substances to be not static but ever changing, in structure as well as fun ...
Control of cytoplasmic pH under anoxic
... of different plants which, growing under different conditions, have different strategies of pH regulation. Thus, the choice of what organisms and cells are studied and with which techniques, not only engenders the problem of oversimplification, but also encourages a certain polarity amongst investig ...
... of different plants which, growing under different conditions, have different strategies of pH regulation. Thus, the choice of what organisms and cells are studied and with which techniques, not only engenders the problem of oversimplification, but also encourages a certain polarity amongst investig ...
biochemistry - Louis Bolk Institute
... How can we do justice to life itself when studying the life sciences? Biochemistry is the area in the life sciences which pre-eminently offers insight into the continuous and manifold changes that occur in organisms. It shows substances to be not static but ever changing, in structure as well as fun ...
... How can we do justice to life itself when studying the life sciences? Biochemistry is the area in the life sciences which pre-eminently offers insight into the continuous and manifold changes that occur in organisms. It shows substances to be not static but ever changing, in structure as well as fun ...
Metabolic implications of methionine excess. Effects of
... Daniel and Waisman (1969) were the only authors to study the adaptation of rats to a dietary excess of methionine, and their work was restricted to the catabolic pathway of this amino acid. Our purpose was to compare the effects of nutritional (fasting, high protein diet and methionine excess) and h ...
... Daniel and Waisman (1969) were the only authors to study the adaptation of rats to a dietary excess of methionine, and their work was restricted to the catabolic pathway of this amino acid. Our purpose was to compare the effects of nutritional (fasting, high protein diet and methionine excess) and h ...
The role of multifunctional M1 metallopeptidases
... appears to act as a receptor and a signalling molecule (reviewed in Mina-Osorio, 2008). However, these functions are likely to include other protein partners. APN acts as receptor for corona viruses (Kolb et al., 1998; Tusell et al., 2007), and appears to function in cholesterol uptake independent o ...
... appears to act as a receptor and a signalling molecule (reviewed in Mina-Osorio, 2008). However, these functions are likely to include other protein partners. APN acts as receptor for corona viruses (Kolb et al., 1998; Tusell et al., 2007), and appears to function in cholesterol uptake independent o ...
University of Groningen Plasticity of airway smooth muscle
... [3H]thymidine incorporation. This was mediated by B2 receptors as determined using the selective B2 receptor antagonist HOE140 and not dependent on cyclooxygenase as determined using indomethacin. Bradykinin-induced synergism with EGF could be suppressed by the protein kinase C (PKC) inhibitors GF10 ...
... [3H]thymidine incorporation. This was mediated by B2 receptors as determined using the selective B2 receptor antagonist HOE140 and not dependent on cyclooxygenase as determined using indomethacin. Bradykinin-induced synergism with EGF could be suppressed by the protein kinase C (PKC) inhibitors GF10 ...
Enzyme Mechanisms
... covalently bonded to substrate carbonyl carbon, moving - charge to the carbonyl O. Oxyanion is on the substrate's oxygen Oxyanion stabilized by additional interaction in addition to the protonated his 57: main-chain NH group from gly 193 H-bonds to oxygen atom (or ion) from the substrate, further st ...
... covalently bonded to substrate carbonyl carbon, moving - charge to the carbonyl O. Oxyanion is on the substrate's oxygen Oxyanion stabilized by additional interaction in addition to the protonated his 57: main-chain NH group from gly 193 H-bonds to oxygen atom (or ion) from the substrate, further st ...
as a PDF
... of these sites . However, none of the reported T kinases is capable ofphosphorylating all of these sites (particularly the site of Ser 262) in PHF-T. The action mechanism for the abnormal phosphorylation of PHF-T, therefore, remains to be established. Protein kinase FA was identified originally as a ...
... of these sites . However, none of the reported T kinases is capable ofphosphorylating all of these sites (particularly the site of Ser 262) in PHF-T. The action mechanism for the abnormal phosphorylation of PHF-T, therefore, remains to be established. Protein kinase FA was identified originally as a ...
De novo lipogenesis in the liver in health and disease: more than
... the substrate for the next round of elongation as it binds the thiol-group of the cysteine at the catalytic site of KS. The elongation ceases at the 16- or 18-carbon stage (Foster & Bloom, 1963; Carey, Dils & Hansen, 1970) with release of palmitic acid or stearic acid from ACP via activity of the th ...
... the substrate for the next round of elongation as it binds the thiol-group of the cysteine at the catalytic site of KS. The elongation ceases at the 16- or 18-carbon stage (Foster & Bloom, 1963; Carey, Dils & Hansen, 1970) with release of palmitic acid or stearic acid from ACP via activity of the th ...
Enzymes of Glycolysis Are Functionally Associated
... aldolase, phosphoglycerate mutase, and enolase) were also identified in an intermembrane space/outer mitochondrial membrane fraction. Enzyme activity assays confirmed that the entire glycolytic pathway was present in preparations of isolated Arabidopsis mitochondria, and the sensitivity of these act ...
... aldolase, phosphoglycerate mutase, and enolase) were also identified in an intermembrane space/outer mitochondrial membrane fraction. Enzyme activity assays confirmed that the entire glycolytic pathway was present in preparations of isolated Arabidopsis mitochondria, and the sensitivity of these act ...
What is Xtend
... protein synthesis is met leucine can then be used as a precursor for alanine and glutamine or to activate various signaling pathways (Layman, 2003), such as the mTOR pathway. It may sound like leucine is free to exert its powerful effect of mTOR activation, but one must remember that protein breakdo ...
... protein synthesis is met leucine can then be used as a precursor for alanine and glutamine or to activate various signaling pathways (Layman, 2003), such as the mTOR pathway. It may sound like leucine is free to exert its powerful effect of mTOR activation, but one must remember that protein breakdo ...
Elucidating Substrate and Inhibitor Binding Sites on the Surface of
... potential substrate binding subsite that can accommodate a variety of amino acid side chains. The predicted positions of Arg (dark blue), Lys (blue), His (cyan), Gln (magenta), Leu (green), Met (orange), Phe (black), Trp (purple) and Tyr (red) bound to the cavity are shown. The Cβ atoms of these sid ...
... potential substrate binding subsite that can accommodate a variety of amino acid side chains. The predicted positions of Arg (dark blue), Lys (blue), His (cyan), Gln (magenta), Leu (green), Met (orange), Phe (black), Trp (purple) and Tyr (red) bound to the cavity are shown. The Cβ atoms of these sid ...
Metabolomics of a Single Vacuole Reveals
... Our method to isolate vacuoles has several advantages over other methods such as the perfusion method, which is also a superior method for isolating a pure, single vacuole from a single cell (Sakano and Tazawa, 1984). However, the perfusion method is more time consuming than our vacuolar isolation m ...
... Our method to isolate vacuoles has several advantages over other methods such as the perfusion method, which is also a superior method for isolating a pure, single vacuole from a single cell (Sakano and Tazawa, 1984). However, the perfusion method is more time consuming than our vacuolar isolation m ...
FC Chimeric Fusion Protein Purified Human
... Fas (APO-1, CD95) is a 45 kDa cell surface protein that mediates apoptosis when cross-linked with agonistic anti-Fas antibodies or Fas ligand (FasL). Fas belongs to the TNF (tumor necrosis factor)/NGF (nerve growth factor) receptor family, and is expressed in various tissues and cells including the ...
... Fas (APO-1, CD95) is a 45 kDa cell surface protein that mediates apoptosis when cross-linked with agonistic anti-Fas antibodies or Fas ligand (FasL). Fas belongs to the TNF (tumor necrosis factor)/NGF (nerve growth factor) receptor family, and is expressed in various tissues and cells including the ...
PDF
... during the first 2 weeks of incubation; similar results have been reported by Shore (1965) in early development of Rana pipiens and R. sylvatica, although some striking changes in these proteins are observed in extracts of homologous organs when tadpoles and frogs of R. catesbeiana are compared (Man ...
... during the first 2 weeks of incubation; similar results have been reported by Shore (1965) in early development of Rana pipiens and R. sylvatica, although some striking changes in these proteins are observed in extracts of homologous organs when tadpoles and frogs of R. catesbeiana are compared (Man ...
A metabolic link to skeletal muscle wasting and regeneration
... suppresses phosphorylation of mTOR and 4E-BP1 and induces atrophy in C2C12 myotubes in vitro (Zhao et al., 2010). On the other hand, knock-down of AMPKα1/2 subunits has been shown to increase myotube diameter, associated with a marked increase in S6K1 and protein synthesis rate (Lantier et al., 2010 ...
... suppresses phosphorylation of mTOR and 4E-BP1 and induces atrophy in C2C12 myotubes in vitro (Zhao et al., 2010). On the other hand, knock-down of AMPKα1/2 subunits has been shown to increase myotube diameter, associated with a marked increase in S6K1 and protein synthesis rate (Lantier et al., 2010 ...
Full-Text PDF
... have found that the triolide of R-3HB forms crown ester complexes with alkali metals; Burger and Seebach [39] have shown that oligomers of R-3HB transport alkali and alkaline earth salts across methylene chloride layers in U-tubes; Seebach et al. [40] have demonstrated that PHB of 16 residues or mul ...
... have found that the triolide of R-3HB forms crown ester complexes with alkali metals; Burger and Seebach [39] have shown that oligomers of R-3HB transport alkali and alkaline earth salts across methylene chloride layers in U-tubes; Seebach et al. [40] have demonstrated that PHB of 16 residues or mul ...
A New Type of a Multifunctional ß
... Figure 1. Most common pathways for the degradation of fatty acid CoA esters by -oxidation enzymes in the different organisms. In the first reaction of the -oxidation cycle, acyl-CoA esters are desaturated to ⌬2-trans enoyl-CoA esters by acyl CoA oxidases or acyl CoA dehydrogenases. Oxidases are lo ...
... Figure 1. Most common pathways for the degradation of fatty acid CoA esters by -oxidation enzymes in the different organisms. In the first reaction of the -oxidation cycle, acyl-CoA esters are desaturated to ⌬2-trans enoyl-CoA esters by acyl CoA oxidases or acyl CoA dehydrogenases. Oxidases are lo ...
Adaptative biochemical pathways and regulatory networks in
... Physiological studies [12], EPS composition and metal-binding activity thereof [17, 18] revealed characteristic peculiarities of BAS-10 strain. Thus, a sequence of 1447 nt gene was generated from BAS-10 16S rDNA (Additional File 1) to perform phylogenetic clustering. Two ClustalW analyses were perfo ...
... Physiological studies [12], EPS composition and metal-binding activity thereof [17, 18] revealed characteristic peculiarities of BAS-10 strain. Thus, a sequence of 1447 nt gene was generated from BAS-10 16S rDNA (Additional File 1) to perform phylogenetic clustering. Two ClustalW analyses were perfo ...
A New Type of a Multifunctional ß-Oxidation
... Figure 1. Most common pathways for the degradation of fatty acid CoA esters by -oxidation enzymes in the different organisms. In the first reaction of the -oxidation cycle, acyl-CoA esters are desaturated to ⌬2-trans enoyl-CoA esters by acyl CoA oxidases or acyl CoA dehydrogenases. Oxidases are lo ...
... Figure 1. Most common pathways for the degradation of fatty acid CoA esters by -oxidation enzymes in the different organisms. In the first reaction of the -oxidation cycle, acyl-CoA esters are desaturated to ⌬2-trans enoyl-CoA esters by acyl CoA oxidases or acyl CoA dehydrogenases. Oxidases are lo ...
Lipid signaling
Lipid signaling, broadly defined, refers to any biological signaling event involving a lipid messenger that binds a protein target, such as a receptor, kinase or phosphatase, which in turn mediate the effects of these lipids on specific cellular responses. Lipid signaling is thought to be qualitatively different from other classical signaling paradigms (such as monoamine neurotransmission) because lipids can freely diffuse through membranes (see osmosis.) One consequence of this is that lipid messengers cannot be stored in vesicles prior to release and so are often biosynthesized ""on demand"" at their intended site of action. As such, many lipid signaling molecules cannot circulate freely in solution but, rather, exist bound to special carrier proteins in serum.