Regulation of Acetyl-Coenzyme A Carboxylase and Acetyl
... m easured according to K uhn et al. [13]. C ontrol sam ples contained neither CoA nor ATP, both of w hich were essential for high rates o f [ l - 14C ]acetate adsorption to the charcoal. Assay o f acetvl-CoA carboxylase Acetyl CoA carboxylase activity in chloroplasts was measured as an acetyl-C oA - ...
... m easured according to K uhn et al. [13]. C ontrol sam ples contained neither CoA nor ATP, both of w hich were essential for high rates o f [ l - 14C ]acetate adsorption to the charcoal. Assay o f acetvl-CoA carboxylase Acetyl CoA carboxylase activity in chloroplasts was measured as an acetyl-C oA - ...
Natural abundance of 15N in amino acids and
... Homogenization of 2 g frozen nodules in 10 ml of 80% (w/v) ethanol for 10 min was followed by centrifugation at 10 000 rpm for 15 min at temperatures below 4 °C. Ethanol in the supernatant was evaporated in a rotary evaporator at 40 °C, and the residue was put on a cation exchange resin (Dowex 50 W, ...
... Homogenization of 2 g frozen nodules in 10 ml of 80% (w/v) ethanol for 10 min was followed by centrifugation at 10 000 rpm for 15 min at temperatures below 4 °C. Ethanol in the supernatant was evaporated in a rotary evaporator at 40 °C, and the residue was put on a cation exchange resin (Dowex 50 W, ...
Phytochemistry
... proteins) of nonimmune origin with no enzymatic activity The Amaranthus extract was purified in a column [l-3]. Due to their specific binding properties, lectins have been used in studies of cell surface architecture, packed with blood group 'A' stroma (Table 1, Fig. 1). As blood-group typing, isola ...
... proteins) of nonimmune origin with no enzymatic activity The Amaranthus extract was purified in a column [l-3]. Due to their specific binding properties, lectins have been used in studies of cell surface architecture, packed with blood group 'A' stroma (Table 1, Fig. 1). As blood-group typing, isola ...
Characterization of transport systems for cysteine, lysine, alanine
... To derive accurate kinetic parameters, it is essential that uptake is relatively constant over the period of measurement. To determine the period in which this assumption holds for the skin strip model, skin strips were incubated as described above in KRP medium for a minimum of 30 min before transf ...
... To derive accurate kinetic parameters, it is essential that uptake is relatively constant over the period of measurement. To determine the period in which this assumption holds for the skin strip model, skin strips were incubated as described above in KRP medium for a minimum of 30 min before transf ...
PDF
... a high yield potential, low fertilization levels its tolerance to flood or salinity, drought survival. This plant is also commonly grown for animal feed and forage. Grass pea seeds may represent a potential source of several important nutrients for human and animal nutrition. On the other hand, the ...
... a high yield potential, low fertilization levels its tolerance to flood or salinity, drought survival. This plant is also commonly grown for animal feed and forage. Grass pea seeds may represent a potential source of several important nutrients for human and animal nutrition. On the other hand, the ...
Test Example
... (a) List the types of noncovalent interactions that are important in providing stability to the threedimensional structures of macromolecules. (b) Why is it important that these interactions be noncovalent, rather than covalent, bonds? Ans: (a) Noncovalent interactions include hydrogen bonds, ionic ...
... (a) List the types of noncovalent interactions that are important in providing stability to the threedimensional structures of macromolecules. (b) Why is it important that these interactions be noncovalent, rather than covalent, bonds? Ans: (a) Noncovalent interactions include hydrogen bonds, ionic ...
Lecture 18 Membranes 1: Lipids and Lipid Bilayers
... Membranes of living cells must be fluid -- must have transition temperatures below body temperature of the organism. Regulation of fluidity (especially in organisms that don’t rigorously control their body temperature) by lipid composition: 1. fatty acid chainlength (shorter → more fluid) 2. number ...
... Membranes of living cells must be fluid -- must have transition temperatures below body temperature of the organism. Regulation of fluidity (especially in organisms that don’t rigorously control their body temperature) by lipid composition: 1. fatty acid chainlength (shorter → more fluid) 2. number ...
Metabolic acidosis
... Initial screening to differentiate the high-AG acidose (1) history for evidence of drug and toxin ingestion and measurement of arterial blood gas to detect coexistent respiratory alkalosis ...
... Initial screening to differentiate the high-AG acidose (1) history for evidence of drug and toxin ingestion and measurement of arterial blood gas to detect coexistent respiratory alkalosis ...
Oxidation and biosynthesis of fatty acids
... • Occurs mainly in liver and adipocytes, in mammary glands during lactation • Occurs in cytoplasm ...
... • Occurs mainly in liver and adipocytes, in mammary glands during lactation • Occurs in cytoplasm ...
Sample pages 2 PDF
... What Figure 2.11 tells us is that a conformational map for a dipeptide of glycine (the side chain in glycine is very small, just a hydrogen) has mostly allowed or partially allowed conformations and therefore polyglycine is flexible. One question that you might ask is how do we know that the conforma ...
... What Figure 2.11 tells us is that a conformational map for a dipeptide of glycine (the side chain in glycine is very small, just a hydrogen) has mostly allowed or partially allowed conformations and therefore polyglycine is flexible. One question that you might ask is how do we know that the conforma ...
ID_4450_General principles of metaboli_English_sem_5
... NAD+ and NADP+ FAD H2 and NADH + H+ FAD and FMN All of these FAD and NAD+ Why is it undesirable to have high concentrations of free fatty acids and lysophosphoglycerides in cells? They are unstable, free radicals that can react to form toxic substances They polymerize easily and can cause the cytoso ...
... NAD+ and NADP+ FAD H2 and NADH + H+ FAD and FMN All of these FAD and NAD+ Why is it undesirable to have high concentrations of free fatty acids and lysophosphoglycerides in cells? They are unstable, free radicals that can react to form toxic substances They polymerize easily and can cause the cytoso ...
MusselsAlive Report
... conditions of seed development, and the ω3/ω6 ratio is an excellent index to compare the relative nutritional value of lipids, where high values generally correspond to better quality foods. Due to the low content of ω6-PUFA, the ω3/ω6 ratios obtained were high (6.5), pointing out an excellent meat ...
... conditions of seed development, and the ω3/ω6 ratio is an excellent index to compare the relative nutritional value of lipids, where high values generally correspond to better quality foods. Due to the low content of ω6-PUFA, the ω3/ω6 ratios obtained were high (6.5), pointing out an excellent meat ...
Nitrification of Oxirne Compounds by Heterotrophic Bacteria
... Hydroxylamine and its aldehyde and ketone derivatives, the oximes, are known to arise in certain microbial processes. Endres (1935), Horner & Burk (1939), Aso, Migita & Ihda (1939), and Virtanen & Laine (1939) found small amounts of oxime in Axotobacter cultures grown with molecular nitrogen, nitrat ...
... Hydroxylamine and its aldehyde and ketone derivatives, the oximes, are known to arise in certain microbial processes. Endres (1935), Horner & Burk (1939), Aso, Migita & Ihda (1939), and Virtanen & Laine (1939) found small amounts of oxime in Axotobacter cultures grown with molecular nitrogen, nitrat ...
Plant cuticles shine: advances in wax biosynthesis
... phenotypes were not additive, suggesting that these gene products may act in the same process or pathway. On the basis of this genetic evidence, and by analogy with the Drosophila eye pigment ABC transporters, a working model has been proposed where heterodimers of ABCG11 and ABCG12 act in wax trans ...
... phenotypes were not additive, suggesting that these gene products may act in the same process or pathway. On the basis of this genetic evidence, and by analogy with the Drosophila eye pigment ABC transporters, a working model has been proposed where heterodimers of ABCG11 and ABCG12 act in wax trans ...
Cloning and Characterization of Human Urocortin
... CAMP accumulation from cells stably transfected with these receptors, and acts in vitro to releaseACTH from dispersedrat anterior pituitary cells. In addition, the CRF-binding protein binds humanurocortin with high affinity and can prevent urocortin-stimulated ACTH secretionin vitro. The inhibitory ...
... CAMP accumulation from cells stably transfected with these receptors, and acts in vitro to releaseACTH from dispersedrat anterior pituitary cells. In addition, the CRF-binding protein binds humanurocortin with high affinity and can prevent urocortin-stimulated ACTH secretionin vitro. The inhibitory ...
FATTY ACID CATABOLISM
... completely oxidized to CO2 in the citric acid cycle, resulting in further energy conservation. In some species and in some tissues, the acetyl-CoA has alternative fates. In liver, acetyl-CoA may be converted to ketone bodies—water-soluble fuels exported to the brain and other tissues when glucose is ...
... completely oxidized to CO2 in the citric acid cycle, resulting in further energy conservation. In some species and in some tissues, the acetyl-CoA has alternative fates. In liver, acetyl-CoA may be converted to ketone bodies—water-soluble fuels exported to the brain and other tissues when glucose is ...
medical chemistry and biochemistry
... 5. Identify anaplerotic reaction of the cycle. L27 Gluconeogenesis 1. Describe the overall purpose of gluconeogenesis, its reactants and products, its cellular localization, and its tissue distribution. 2. Differentiate the enzymes involved in glycolysis vs gluconeogenesis. 3. Explain the contribut ...
... 5. Identify anaplerotic reaction of the cycle. L27 Gluconeogenesis 1. Describe the overall purpose of gluconeogenesis, its reactants and products, its cellular localization, and its tissue distribution. 2. Differentiate the enzymes involved in glycolysis vs gluconeogenesis. 3. Explain the contribut ...
glucuronidation of opioids, carboxylic acid
... in the endoplasmic reticulum and participate in the metabolic elimination of many endogenous compounds and xenobiotics (1). Compounds with a wide variety of chemical moieties, such as amines, hydroxylated compounds, and carboxylic acids, are substrates for UGT isoforms. UGTs that are members of the ...
... in the endoplasmic reticulum and participate in the metabolic elimination of many endogenous compounds and xenobiotics (1). Compounds with a wide variety of chemical moieties, such as amines, hydroxylated compounds, and carboxylic acids, are substrates for UGT isoforms. UGTs that are members of the ...
Assessment of the mathematical issues involved
... experiment ends. This assumption might be of slight concern during a prolonged tracer infusion for slowly turning over pools, because there may be some recycling of tracer. This assumption is reasonable in the flooding dose situation for muscle because all sample collection is complete within 2 h. F ...
... experiment ends. This assumption might be of slight concern during a prolonged tracer infusion for slowly turning over pools, because there may be some recycling of tracer. This assumption is reasonable in the flooding dose situation for muscle because all sample collection is complete within 2 h. F ...
26_Catabolism of tryacylglycerols oxidation of fatty acids a
... acids and monoacylglycerol •Monoacylglycerol is hydrolyzed to fatty acid and glycerol or by a hormone-sensitive lipase or by more specific and more active monoacylglycerol lipase ...
... acids and monoacylglycerol •Monoacylglycerol is hydrolyzed to fatty acid and glycerol or by a hormone-sensitive lipase or by more specific and more active monoacylglycerol lipase ...
Document
... What is an amino acid? Twenty different kinds of amino acids are used by living organisms to produce proteins An amino acid is a molecule containing an amine (-NH2) an acid (-COOH) and a third chemical group (-R) that defines the amino acid. In glycine, the simplest amino acid, R is –H, or a hydrog ...
... What is an amino acid? Twenty different kinds of amino acids are used by living organisms to produce proteins An amino acid is a molecule containing an amine (-NH2) an acid (-COOH) and a third chemical group (-R) that defines the amino acid. In glycine, the simplest amino acid, R is –H, or a hydrog ...
Regulation of Primary Metabolism in Response to
... Based on enzyme activity assays and metabolic responses to waterlogging of the legume Lotus japonicus, it was previously suggested that, during hypoxia, the tricarboxylic acid cycle switches to a noncyclic operation mode. Hypotheses were postulated to explain the alternative metabolic pathways invol ...
... Based on enzyme activity assays and metabolic responses to waterlogging of the legume Lotus japonicus, it was previously suggested that, during hypoxia, the tricarboxylic acid cycle switches to a noncyclic operation mode. Hypotheses were postulated to explain the alternative metabolic pathways invol ...
The Puzzle of the Krebs Citric Acid Cycle: Assembling the Pieces of
... enone could take place. A substitution reaction on the feeder could also occur. Consider, for example, the first possibility mentioned; then many different keto compounds could participate in this addition reaction. This problem arises again in searching the next sequence of reactions, since the con ...
... enone could take place. A substitution reaction on the feeder could also occur. Consider, for example, the first possibility mentioned; then many different keto compounds could participate in this addition reaction. This problem arises again in searching the next sequence of reactions, since the con ...
Direction of Krebs cycle Which way does the citric acid cycle turn
... Since Krebs' discovery of the citric acid cycle, some of the remaining steps of the cycle have been proven to operate reversibly and in segments, the directionalities of which are dictated by the presence or absence of oxygen. To address this in more detail these steps are examined individually in c ...
... Since Krebs' discovery of the citric acid cycle, some of the remaining steps of the cycle have been proven to operate reversibly and in segments, the directionalities of which are dictated by the presence or absence of oxygen. To address this in more detail these steps are examined individually in c ...
Hepoxilin
Hepoxilins (HxA3 and HxB3) are nonclassic eicosanoid hormones involved in inflammation.