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LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034 B.Sc. DEGREE EXAMINATION – PLANT BIOLOGY & PLANT BIO-TECH. FIFTH SEMESTER – APRIL 2012 PB 5406 - IMMUNOLOGY AND IMMUNOTECHNOLOGY Date : 30-04-2012 Time : 1:00 - 4:00 Dept. No. Max. : 100 Marks Part –A Answer all, each answer within 50 words: (10x2=20 marks) 1. What is agglutination? 2. Define cell mediated immunity. 3. Define Haptens. 4. What is the function of suppressor T cells? 5. Define epitope. 6. How hybridomas are selected? 7. Distinguish between cytokines and hormones. 8. Define antigens. 9. What are the two pathways involved in monoclonal antibodies? 10. What are cytotoxic T cells? Part B Answer the following each answer within 500 words. Draw diagrams wherever necessary: (5x7=35 marks) 11. a) Explain innate and adaptive immunity. OR b) How will you isolate serum from blood? 12. a) Write short notes on: i) agglutination inhibition ii) complement fixation OR b) Explain radioimmunoassay technique. 13. a) Explain the properties of Cytokines. OR b) Describe the steps involved in western blot. 14. a) Explain T-lymphocytes. OR b) Write about primary and secondary immune response? 15. a) Differentiate polyclonal antibodies from monoclonal antibodies. OR b) Explain the pathways involved in hybridoma technology. Part C Answer any three of the following each answer within 1200 words. Draw diagrams wherever necessary: (3x15=45 marks) 16. Explain the different types of immunoglobulins. 17. Explain antigen-antibody interaction. 18. Discuss the applications of monoclonal antibodies. 19. Explain the types and role of cytokines in immunity and mention its functions. 20. Define and explain cell mediated and humoral immunity. ******************