Download LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
M.Sc. DEGREE EXAMINATION – BIO TECHNOLOGY
FIRST SEMESTER – NOVEMBER 2007
AE 18
BT 1816 - IMMUNOLOGY
Date : 02/11/2007
Time : 1:00 - 4:00
Dept. No.
Max. : 100 Marks
PART – A
Answer ALL questions:
I. Choose the best answer out of the given choices.
(5 x 1 = 5)
1. Which of the following event does not occur during apoptosis?
a) Chromatin compaction
b) Nuclear fragmentation
c) Flocculent mitochondria
d) Cytoplasmic condensation
2. Mast cells are derived from _______________ progewnitor.
a) Eosinophil
b) Basophil
c) Monocyte
d) Erythroid
3. Which type of antibody molecule possesses the highest avidity?
a) Ig G
b) Ig M
c) Ig A
d) Ig D
4. An increased OD value indicates no infection in ______________ ELISA.
a) Competitive
b) Sandwich
c) Indirect ELISA
d) Direct
5. Which of the following molecule is not associated with endogenous antigen processing pathway?
a) TAP
b) Proteasome
c) Molecular chaperones
d) CLIP
II. State true or false; if false, give reasons.
(5 x 1 = 5)
6. The macrophages present in the kidney are called kupffer cells.
7. Phagolysosome formation is associated with exogenous mode of antigen processing.
8. Native forms of antigens are more immunogenic than denatured antigens.
9. Monoclonal antibodies take longer time span to be produced and are labour intensive as well.
10. Grave’s disease is an example of autoimmune disorder.
III. Complete the following.
(5 x 1 = 5)
11. _______________ discovered the role of phagocytosis in immunity.
12. Colony-stimulating factors are cytokines involved in the regulation of __________________.
13. ________________ ELISA is also called as class-capture ELISA.
14. ________________ is the first commercial monoclonal antibody approved by US-FDA.
15. ________________ immunization prevents infection at the site of pathogen entry itself.
IV. Define the following, each in about 50 words.
16. CDR
17. Affinity maturation
18. Endotoxin shock
19. Catalytic antibodies
20. Titre value
(5 x 1 = 5)
PART – B
V. Answer any FIVE of the following questions, each in about 350 words:
(5 x 8 = 40)
21. Tabulate the differences between the five classes of immunoglobulins.
22. Give an outline classification of immunity in the host system.
23. Explain the principle behind HAT media selection during the production of monoclonal antibodies.
24. How does the exogenous antigen processing pathway operate in the body to eliminate bacteria?
25. Describe in brief about the immune response against any one parasitic infection of your choice. Focuss
on both innate and acquired responses.
26. Write the principle and procedure of Ouchterlony Double Diffusion test. How will you interpret the
resulting precipitin patterns?
27. Comment on recombinant vector vaccines’ and DNA vaccines’ contribution to the field of vaccinology.
28. Bring out the recent advancements in the area of transplantation immunology.
PART – C
VI. Answer the following, each in about 1200 words:
(2 x 20 = 40)
29. a) What is hematopoiesis? List down and describe the salient features of both
lymphoid and myeloid lineage of cells.
(4+8+8)
(or)
b) Define an antigen. What are the various factors influencing immunogenicity?
Also, add a note on haptens.
(2+12+6)
30. a) ‘ELISA is an efficient diagnostic tool in immunotechnology’ – Elucidate this
statement, with special reference to the types of ELISA and their specific applications.
(or)
b) Write an essay on the applications of monoclonal antibodies in cancer diagnosis and treatment.
(10+10)
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