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Anthony C. Forster Department of Cell and Molecular Biology Uppsala University, Sweden SEMINAR May, 11th 14.30 – Room A206 Faculty of Science Via Sommarive, 5 Povo, Trento Applications of Protein Synthesis in Synthetic Biology and Drug Discovery We have developed a simplified, purified, bacterial translation system to facilitate studies of substrate recognition in protein synthesis and enable new applications. One application is directed evolution in vitro of small-molecule, peptidomimetic drug candidates by redesigning the genetic code for the synthesis and display of polymers containing unnatural amino acids (Forster et al. 2003, 2004). This technology was recently commercialized by two start-up biotech companies (Ra Pharmaceuticals Inc. and PeptiDream Inc.). Another application is construction of scalable, purified, in vitro translation systems. This is proceeding by “BioBrick” construction in E. coli using orthogonal replication, promoter and terminator parts (Du et al. 2011). A long-term goal is achieving better understanding and manipulation of biological replication by reconstituting it completely in vitro. Of the 151 genes postulated to be necessary for self-replication from small molecules (synthetic life), protein synthesis constitutes 96% (Forster and Church, 2006). CIBIO Via delle Regole, 101 Mattarello – Trento Tel. 0461 282742 [email protected]
