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Chapter 28: Prokaryotes • • • • • • Prokaryotes are cellular and therefore are living organisms. Significance: oldest, simplest, and most abundant form of life important for ecology (photosynthesis, recycling, symbiotic relationships, nitrogen fixing, and bioremediation) can cause disease by either: (a) destroying cells or (b) producing toxins May be treated with antibiotics (Ex: penicillin) Classification into two groups: Archaea and Bacteria (or Eubacteria) Basic cell shapes: bacillus (rod-shaped) coccus (spherical) spirillum (spiral or helical) *** Gram stain can be used to help identify bacteria and then also help with the choice of antibiotics. Size: about 1 micron (micron = 0.000001 m) Structure of bacteria: See Fig. 28.3 (p. 542) basic structures: cell wall plasma membrane cytoplasm containing ribosomes DNA chromosome (circular) in a nucleoid some also have: gelatinous capsule flagella for movement pili for attaching to surfaces plasmids - extra pieces of DNA Fig. 28.11.b Presence of F plasmid (conjugative plasmid) allows conjugation in prokaryotes Fig. 28.14(left) Fig. 28.14(right) Generalized transduction – Viruses pack host DNA and transfer it upon subsequent infection. Specialized transduction is shown by phage exhibiting lysogenic life cycle Fig. 28.15 Natural transformation- One cell dies and releases its contents into environment. Prokaryotic metabolism • Autotrophs or heterotrophs obtain carbon and energy through light and chemical reactions • Photoautotrophs carry out photosynthesis and obtain carbon from carbon dioxide • Chemolithoautotrophs obtain energy by oxidizing inorganic substances • Photoheterotrophs use light for energy but obtain carbon from organic molecules • Chemoheterotrophs obtain carbon and energy from organic molecules Bacterial Diseases • Transfer sources – Mucus or saliva droplets, contaminated food and water and insect vectors • Causes Tuberculosis • Tooth decay • Helicobacter pylori causes ulcers • Causes sexually transmitted diseases gonorrhea, syphilis and chlamydia • Gram-negative bacteria (pseudomonads) – plant diseases This project is funded by a grant awarded under the President’s Community Based Job Training Grant as implemented by the U.S. Department of Labor’s Employment and Training Administration (CB-15-162-06-60). NCC is an equal opportunity employer and does not discriminate on the following basis: against any individual in the United States, on the basis of race, color, religion, sex, national origin, age disability, political affiliation or belief; and against any beneficiary of programs financially assisted under Title I of the Workforce Investment Act of 1998 (WIA), on the basis of the beneficiary’s citizenship/status as a lawfully admitted immigrant authorized to work in the United States, or his or her participation in any WIA Title I-financially assisted program or activity. “This workforce solution was funded by a grant awarded under the President’s Community-Based Job Training Grants as implemented by the U.S. Department of Labor’s Employment and Training Administration. The solution was created by the grantee and does not necessarily reflect the official position of the U.S. Department of Labor. The Department of Labor makes no guarantees, warranties, or assurances of any kind, express or implied, with respect to such information, including any information on linked sites and including, but not limited to, accuracy of the information or its completeness, timeliness, usefulness, adequacy, continued availability, or ownership. This solution is copyrighted by the institution that created it. Internal use by an organization and/or personal use by an individual for non-commercial purposes is permissible. All other uses require the prior authorization of the copyright owner.”