* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Download What are the three steps in PCR?
DNA sequencing wikipedia , lookup
Metagenomics wikipedia , lookup
Zinc finger nuclease wikipedia , lookup
Genetic engineering wikipedia , lookup
Designer baby wikipedia , lookup
Comparative genomic hybridization wikipedia , lookup
Site-specific recombinase technology wikipedia , lookup
SNP genotyping wikipedia , lookup
Therapeutic gene modulation wikipedia , lookup
United Kingdom National DNA Database wikipedia , lookup
Genome editing wikipedia , lookup
Gel electrophoresis of nucleic acids wikipedia , lookup
Bisulfite sequencing wikipedia , lookup
Nucleic acid analogue wikipedia , lookup
Non-coding DNA wikipedia , lookup
Restriction enzyme wikipedia , lookup
Genomic library wikipedia , lookup
Artificial gene synthesis wikipedia , lookup
Vectors in gene therapy wikipedia , lookup
DNA supercoil wikipedia , lookup
Transformation (genetics) wikipedia , lookup
DNA vaccination wikipedia , lookup
Cre-Lox recombination wikipedia , lookup
What are the three steps in PCR? What are the three steps in PCR? Denaturation Hybridization of Primer DNA replication What is the enzyme used for PCR? What is the enzyme used for PCR? DNA Polymerase from a hyperthermophile This is why the enzyme is not destroyed at 94 degrees Celsius What are introns? What are introns? Introns are non-coding sequences found in structural genes in eukaryotes They are removed during splicing What is reverse transcriptase? What is reverse transcriptase? It is an enzyme that copies RNA into DNA It is produced by certain viruses What are restriction enzymes? What are restriction enzymes? They are enzymes that cut DNA at specific base sequences They are produced by bacteria Many of them cut the DNA so that they produce “sticky ends” or single stranded ends What are vectors used in making recombinant DNA? What are vectors used in making recombinant DNA? Vectors are self replicating pieces of DNA They are used to get DNA into cells Two commonly used vectors are plasmids and bacteriophage What are three techniques used to get recombinant DNA into cells? What are three techniques used to get recombinant DNA into cells? Transformation Electroporation Microinjection (for eukaryotic cells) What does RFLP stand for? What does RFLP stand for? Restriction fragment length polymorphism It is often used in DNA fingerprinting It requires gel electrophoresis which separates DNA by size