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TaqI EcoRV SlSERK1 Probe HindIII EcoRV Kb BglII SlSERK3B Probe Kb 10 8 6 5 4 10 8 SlSERK1 6 SlSERK3A 5 SlSERK3B 4 3 3 2 2 1.5 1.5 1 1 Figure S3. DNA-blot analysis of SlSERK family members in tomato cv. Motelle. Genomic DNA, 5 µg, was digested with the indicated restriction enzymes and DNA blots were prepared according to standard protocols. The blots were hybridized with a 32P labeled probe in 50% (v/v) formamide at 42ºC. Final blot washes for SlSERK3B and SlSERK1 were performed in 1X SSC, 0.1% SDS at 42ºC and at 65ºC, respectively. The SlSERK3B probe consists of a cDNA fragment spanning nucleotides 12191536 in the kinase domain. The SlSERK1 probe consists of a cDNA fragment spanning nucleotides 318-610 in LRR3-LRR5 region. In agreement with the tomato genome analysis, the band patterns with SlSERK3B probe revealed the presence of 3 members of SERK genes in tomato. The SlSERK1 probe detected only fragments expected to correspond to SERK1. Arrows point to the SlSERK member with the matching gene color.