Download Human Saliva Amylase Alpha

AR00016PU-N
OriGene Technologies Inc.
9620 Medical Center Drive, Ste 200
Rockville, MD 20850
UNITED STATES
Phone: +1-888-267-4436
Fax: +1-301-340-8606
[email protected]
OriGene EU
Acris Antibodies GmbH
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32052 Herford
GERMANY
Phone: +49-5221-34606-0
Fax: +49-5221-34606-11
[email protected]
Human Saliva Amylase Alpha
Alternate names:
1, 4-alpha-D-glucan glucanohydrolase 1, AMY1A, Salivary alpha-amylase
Catalog No.:
AR00016PU-N
Quantity:
1 kU
Concentration:
0.18 mg Protein/mg powder (Coomassie)
Background:
Amylases are secreted proteins that hydrolyze 1,4 alpha glucoside bonds in
oligosaccharides and polysaccharides. They catalyze the first step in digestion of dietary
starch and glycogen. Alternative splicing results in multiple transcript variants encoding
the same protein.
Salivary Amylase is a digestive enzyme secreted by salivary glands, it consists of a single
polypeptide chain with a molecular weight of 56kDa (family B) or 62kDa (family A), the
difference in molecular weight being due to the presence or absence of a carbohydrate
moiety.
The total serum concentration of amylase is about 120g/L of which about 50% is salivary
amylase.
Although salivary amylase and pancreatic amylase share 95% amino acid sequence
homology, they differ in molecular size, isoelectricpoint and antigenic properties.
Uniprot ID:
P04745
NCBI:
NP_001008219.1
GeneID:
276
Species:
Human
Source:
Saliva, Human Saliva
Format:
State: Lyophilized purified protein
Purity: >90% by SDS-PAGE
Buffer System: 150mM Sodium Chloride, 20mM Tris, 1mM Calcium Chloride, pH 7.2 without
preservatives.
Reconstitution: Restore with Tris saline or other buffer to desired concentration.
Description:
Amylase alpha, Human Saliva. Contaminants: Lipase: < 0.001% Proteases: < 0.001%
Ammonia: < 0.0121 Umole/mg
Specific Activity: 106.2 Units/mg powder at 37°C.
590 Units/mg Protein.One unit will catalyze the hydrolysis of one micromole of malto
pentaose, which through coupled reactions results in the formation of 5 micromoles of
glucose-6-phosphate per minute at 37°C. Measured at 340nm as an equimolar amount of
NADH produced by a coupled reaction.
Storage:
Prior to and following reconstitution store the protein at -20°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
For research and in vitro use only. Not for diagnostic or therapeutic work.
Material Safety Datasheets are available at www.acris-antibodies.com or on request.
Acris Antibodies is now part of the OriGene family. Learn more at www.origene.com
OG/20121030
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AR00016PU-N: Human Saliva Amylase Alpha
Caution:
The donors of the source materials have tested negative for HIV1, HIV2, HCV antibodies and
HBsAg, HIV-1 Antigen and Syphilis. No test guarantees a product to be non-infectious.
Therefore, all material derived from human fluids or tissues should be considered as
potentially infectious.
For research and in vitro use only. Not for diagnostic or therapeutic work.
Material Safety Datasheets are available at www.acris-antibodies.com or on request.
Acris Antibodies is now part of the OriGene family. Learn more at www.origene.com
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