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ULM School of Health Professions
Spring 2017
COURSE TITLE: MLSC 3030, Medical Microbiology I
I.
Contact Information
Instructor:
Jessica Lasiter, MLS (ASCP)CM
Phone:
318.342.1631
Email:
[email protected]
Webpage:
www.ulm.edu/mls
Office:
Sugar 253
Office Hours:
1:00 p.m. - 3:00 p.m. Monday – Wednesday
7:30 a.m. - 11:30 a.m. Friday
Other hours available by appointment
Preferred mode of communication with instructor: email
I.
Course Netiquette Expectations
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II.
Always think before you write. So please think twice before you hit submit.
Keep it relevant. There are places to chat and post for fun everyday stuff. Do not
stray from the discussion in the assigned questions.
Never use all caps. This is the equivalent of yelling in the online world. It is not
fun to read. Only use capital letters when appropriate.
Make sure that you are using appropriate grammar and structure. In other
words, I don’t want to see anyone writing “R U” instead of “are you”. There are
people in the class that may not understand this type of abbreviation, not to
mention it does nothing to help expand your writing and vocabulary skills.
Emoticons are fine as long as they are appropriate. A smile
is welcome,
anything offensive is not.
Treat people the same as you would face-to-face. In other words, it is easy to
hide behind the computer. In some cases, it empowers people to treat others in
ways they would not in person. Remember there is a person behind the name on
your screen. Treat all with dignity and respect and you can expect that in return.
Email Policy
Various services on campus require official “warhawks” student email, and will
not accept a third party account such as Yahoo or Google. The myULM
portal (http://my.ulm.edu) is the best way to receive important notices and to
know about activities on campus.
Warhawks.ulm.edu (students) and ulm.edu (faculty) are considered the official
university email address. When conducting university business through email,
please use only a student’s warhawks.ulm.edu account and/or faculty’s
ulm.edu account.
II.
Course Description
Lasiter MLSC 3030:1701
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ULM School of Health Professions
Spring 2017
4 cr. The morphology, physiology, and immunogenicity of disease-causing bacteria,
methods of isolation, identification, susceptibility testing, and control of pathogenic
bacteria and diseases. Three hours lecture and one hour laboratory.
III.
Course Prerequisites/Corequisites
MLSC majors only
IV.
Course Objectives (Lecture)
A. Define these terms or phrases: microbe, bacteria, virus, parasite, fungus, prion,
rickettsia, virulence, virulence factors, mode of transmission.
B. Discuss the major events in the history of medical microbiology.
C. State the Gram staining reaction, shape, and virulence factors of the major
bacterial pathogens of man.
D. State the principle of the major diagnostic test in the microbiology laboratory.
E. List the various health hazards inherent in the microbiology laboratory and
discuss how they can be minimized.
F. List the steps in the Gram staining procedure and state the principle.
G. Define “antibiotic resistance” and discuss how this can occur in the microbial
world.
H. Define “sensitivity testing” and describe the various methods to perform this
analysis.
I. Describe the proper way to obtain isolated bacterial colonies.
J. List the major types of culture media and state the utility of each.
K. Describe the proper way to “read culture plates.”
L. Describe the performance and interpretation of miniaturized biochemical test
batteries such as the Analytical Profile Index.
Course Objectives (Laboratory)
A. Perform a “streak plate” procedure to obtain isolated bacterial colonies.
B. Evaluate a culture plate containing bacterial growth and determine which colonies
should be worked up for identification and antibiotic sensitivity studies.
C. Examine a Gram stain smear and identify the Gram Stain reaction and
morphology of the bacteria.
D. Perform an antibiotic sensitivity procedure on an isolated bacterial colony to
determine which antibiotics are effective against the organism.
E. Perform a Gram stain on a bacterial smear to determine its Gram stain reaction
and shape.
F. Demonstrate the correct biochemical methods for identifying pathogens
G. Demonstrate setting up an Analytical Profile Index procedure to identify bacteria
biochemically.
H. Perform acid-fast bacilli stains.
* Detailed objectives will be provided for each lecture.
V.
Course Topics
Introduction to Medical Microbiology, The Gram Positive Cocci, Neisseria (Gram
Negative Diplococci), Haemophilus (Tiny Gram Negative Bacilli),
Enterobacteriaceae (Fermentative Gram Negative Bacilli), Nonfermentative Gram
Negative Bacilli, Miscellaneous Gram Negative Bacilli, Aerobic Gram-Positive Non-
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ULM School of Health Professions
Spring 2017
Spore Forming Bacilli, The Aerobic Gram-Positive Spore-Forming Bacilli (Bacillus
Genus), Anaerobic bacteria, Spirochetes and Curved Rods, Mycobacteria, Rickettsia,
Mycoplasma and Ureaplasma
VI.
Instructional Methods and Activities
The instructional methods and activities of this course will include lecture, video
presentation of pertinent concepts, slide presentations, case history studies, class
discussion, objective examinations, pop tests, practical examinations and notebook
construction. Oral presentations, written critiques of video presentations, and
research projects may also be used.
VII.
Evaluation and Grade Assignment
A. Lecture Grade Average
The lecture grade average will be determined by the student’s scores on scheduled
chapter examinations, pop test results, student projects and the final examination
(comprehensive). There will be approximately five comprehensive lecture
examinations and the final comprehensive examination. The lecture grade
average comprises 75% of the final course grade. The final exam is
comprehensive and counts as 20% of the lecture grade average.
Laboratory Grade Average
The laboratory grade average is derived from the laboratory journal grade, quizzes
and practicals and counts as 20% of the final course grade. The total points earned
divided by the total points possible will be used to calculate the laboratory grade
average. The final laboratory exam is comprehensive and counts as 20% of the
laboratory grade average.
Beginning with Week 2, a quiz on the previous week's lab procedures and lectures
will be given at the beginning of the class period, followed by a lecture on the
laboratory procedure for that week. Preparing for the lab exercise by reviewing
the reading assignment before each lecture will help you have a better
understanding of the material presented. Each procedure performed in the
laboratory will be documented in a laboratory journal. Instructions for the journal
are supplied on a separate document. Practical examination will be given
throughout the semester. In order to progress in this course, a 75% on each
practical must be achieved.
Affective Grade Average
An affective evaluation shall determine 5% of the final grade. The student begins
with 100% for the affective evaluation. 10 points are subtracted for each violation
of proper behavior.
B. Grading Scale
A = 93%–100%
B = 84%–92%
C = 76%-83%
Lasiter MLSC 3030:1701
D = 70%-75%
F = 69% and below
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ULM School of Health Professions
Spring 2017
C. Undergraduate mid-term grades will be posted on-line for students to view via
Banner. Mid-term grades indicate the student’s status at mid-semester only and
do not indicate the final performance outcome of the student.
D. Students MUST earn at least a C in all medical laboratory science courses to
progress in the professional program. A non-progressive grade of D will
necessitate remediation of the course according to the MLS professional
program’s remediation policy. A failing grade of F will result in suspension
from the professional program.
E. Students must complete course work in those courses where “I” grades have been
earned within the first month of the following semester or within the first two
weeks of a summer term in which they resume studies at ULM or elsewhere.
The student has one calendar year to make arrangements to have the “I” grade
removed. If the “I” grade is not removed in the specified time, the “I” is
calculated as an “F” into the cumulative grade point average by The University of
Louisiana at Monroe Registrar.
VIII.
Class Policies and Procedures
A. Textbook(s) and Materials
Required
Kiser, K. M., Payne, W. C., Taff, T. A. (2011). Clinical Laboratory
Microbiology: A Practical Approach. Boston: Pearson. ISBN 13: 978-0013-092195-6
University of Louisiana at Monroe Medical Laboratory Science Student
Handbook
Speckled Journal
Colored Pencils
Pencil
Sharpie
Lab Coat
B. Attendance Policy:
Class attendance is regarded as an obligation as well as a privilege, and all
students are expected to know attendance regulations and to attend regularly and
punctually all classes in which they are enrolled. Failure to do so may jeopardize
a student’s scholastic standing and may lead to suspension from the university.
Attendance records are subject to inspection by appropriate college or university
officials.
C. Make-up Policy
Due to the format of this course, all assignments and projects are due on the stated
date and will not be accepted late. If the student has an excusable reason for not
attending class, proper documentation must be provided to the instructor.
Lasiter MLSC 3030:1701
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ULM School of Health Professions
Spring 2017
Documentation is mandatory! Students will follow the policy outlined in the
current Undergraduate Catalog and the Student Handbook.
D. Academic integrity
Faculty and students must observe the ULM published policy on Academic
Dishonesty (see current copy of ULM Student Policy Manual http://www.ulm.edu/studentpolicy/).
E. Course Evaluation Policy
Students are expected to complete the course evaluation available on-line and the
clinical instructor(s) evaluation and clinical facility/department evaluation forms
in the Student Handbook.
F. Student Services
The University of Louisiana at Monroe strives to serve students with special
needs through compliance with Sections 504 of the Rehabilitation Act of 1973
and the Americans with Disabilities Act. These laws mandate that postsecondary
institutions provide equal access to programs and services for students with
disabilities without creating changes to the essential elements of the curriculum.
While students with special needs are expected to meet our institution's academic
standards, they are g
iven the opportunity to fulfill learner outcomes in alternative ways. Examples of
accommodations may include, but are not limited to, testing accommodations
(oral testing, extended time for exams), interpreters, relocation of inaccessible
classrooms, permission to audiotape lectures, note-taking assistance, and course
substitutions.
Information about ULM student services can be found via these links:
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Student Success Center: http://www.ulm.edu/studentsuccess/
Counseling Center http://www.ulm.edu/counselingcenter/
Special Needs at http://www.ulm.edu/studentaffairs/
Library http://www.ulm.edu/library/reference.html
Computing Center Help Desk http://www.ulm.edu/computingcenter/helpdesk
Current college’s policies on serving students with disabilities can be obtained on
the ULM website: http://ulm.edu/counselingcenter/
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If you need accommodation because of a known or suspected disability, you
should contact the Director for Disabled Student Services at:
Voice phone: 318-342-5220
Fax: 318-342-5228
Lasiter MLSC 3030:1701
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ULM School of Health Professions
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Spring 2017
Walk-In: ULM Counseling Center, 1140 University Avenue (this building and
room are handicapped accessible).
Mental Wellness on the ULM Campus
If you are having any emotional, behavioral, or social problems and would like to
talk with a caring, concerned professional, please call one of the following
numbers:
 The ULM Counseling Center: 318-342-5220
 The Marriage and Family Therapy Clinic: 318- 342-9797
 The Community Counseling Center: 318-342-1263
Remember that all services are offered free to students, and all are strictly
confidential.
If you have special needs that I need to be made aware of, you should
contact me within the first two days of class.
Sexual Harassment or Gender-Based Discrimination
Title IX of the Education Amendments of 1972 prohibits sex discrimination
against any participant in an educational program or activity that receives
federal funds, including federal loans and grants. Furthermore, Title IX
prohibits sex discrimination to include sexual misconduct, sexual violence,
sexual harassment and retaliation. If you encounter sexual harassment or
gender-based discrimination, please contact the Title IX Coordinator at
318-342-1004; you may also file a complaint online, 24 hours a day, at:
www.ulm.edu/titleix.
G. Emergency Procedures
 Students will follow the emergency procedures of the facility in which the
practicum is completed.
 University Police Phone: 318.342.5350
 In case of an emergency, dial 911
H. Discipline/Course Specific Policies
See Student Handbook for affective objectives and evaluation form, attendance
policy, grading policy and discipline policy. Tardiness is unacceptable. Each
student is responsible for arriving to class on time. Each tardy will be
documented, and three tardies by a student will convert into one unexcused
absence. It is the student’s responsibility to inform the instructor of extenuating
circumstances that caused the student to be tardy.
Cell phones and pagers must be turned off or silenced during class. If there is an
emergency, the student must leave the classroom and not disrupt the class or
instructor. Violation of this expectation may result in the student’s grade being
lowered.
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ULM School of Health Professions
Spring 2017
Class disruption: When the instructor begins the lecture, all talking MUST cease.
Students who disrupt class will be asked to leave. Chronic classroom disruption
will result in the student being removed from the class roster.
IX
Tentative Course Schedule
Instructor:
Jessica Lasiter, MLS (ASCP)CM
Phone:
318.342.1631
Email:
[email protected]
Webpage:
www.ulm.edu/mls
Office:
Sugar 253
Office Hours: 1:00 p.m. - 3:00 p.m. Monday – Wednesday
7:30 a.m. - 11:30 a.m. Friday
Other hours available by appointment
Lasiter MLSC 3030:1701
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ULM School of Health Professions
Date
Spring 2017
MICROBIOLOGY LECTURE TENTATIVE SCHEDULE
Day
Subject
Reading
17-Jan
Tu
19-Jan
24-Jan
26-Jan
31-Jan
2-Feb
7-Feb
9-Feb
14-Feb
16-Feb
21-Feb
23-Feb
28-Feb
2-Mar
Th
Tu
Th
Tu
Th
Tu
Th
Tu
Th
Tu
Th
Tu
Th
7-Mar
Tu
9-Mar
14-Mar
16-Mar
21-Mar
23-Mar
28-Mar
30-Mar
4-Apr
6-Apr
11-Apr
13-Apr
18-Apr
20-Apr
25-Apr
27-Apr
2-May
4-May
Th
Tu
Th
Tu
Th
Tu
Th
Tu
Th
Tu
Th
Tu
Th
Tu
Th
Tu
Th
Intro, Taxonomy, Bacterial Cell, Host Encounter
& Safety
Specimen Collection & Cultivation
Presumptive Identification
Final Identification
Exam I
Gram Positive Cocci
Gram Positive Cocci cont.
Gram Negative Cocci
Gram Positive Rod
Fastidious Gram Negative Rod
Exam II
Enterobacteriacea
Mardi Gras Break
Enterobacteriacea cont.
Nonfermenter Gram Negative Rods, Vibrio,
Aeromonas, & etc.
Vibrio, Aeromonas, Plesiomonas, etc.
Exam III
Anaerobes
Spirochetes
Miscellaneous Microorganisms
Mycoplasma and Ureaplasma
Exam IV
Chapters 1-5
Chapter 6 & 7
Chapter 8
Chapter 9
Chapter 17
Chapter 17
Chapter 18
Chapter 19
Chapter 20
Chapter 21
Chapter 21
Chapter 22
Chapter 23
Chapter 24
Chapter 25
Chapter 27
Chapter 28
LSCLS Spring Meeting
Susceptibility Testing
Susceptibility Testing
Chapter 11
Chapter 11
Spring Break
Immunologic; Emerging Technologies
Mycobacterium species
Global Threats
Exam V
Chapter 10 & 12
Chapter 26
Chapter 42
Final Exam – May 9, 2017 @ 10am
**The instructor reserves the right to adjust the schedule as needed.
Lasiter MLSC 3030:1701
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ULM School of Health Professions
Spring 2017
MICROBIOLOGY LABORATORY TENTATIVE SCHEDULE
Date Day
Procedures
17-Jan
19-Jan
24-Jan
26-Jan
Tu
Th
Tu
Th
31-Jan
Tu
2-Feb
7-Feb
9-Feb
14-Feb
16-Feb
21-Feb
23-Feb
28-Feb
2-Mar
Th
Tu
Th
Tu
Th
Tu
Th
Tu
Th
7-Mar
Tu
9-Mar
Th
14-Mar
16-Mar
21-Mar
23-Mar
28-Mar
30-Mar
4-Apr
6-Apr
11-Apr
13-Apr
18-Apr
20-Apr
25-Apr
27-Apr
2-May
4-May
Gram Stains
St. Francis Medical Center Lecture
Gram Stains
Gram Stain Practical
Staphylococcus/Streptococcus (Gram Stains, Catalase, Coagulase,
Bile Solubility, PYR)
Staphylococcus/Streptococcus (BE, CAMP, NaCL, Taxo A & P)
Gram Positive Rods (Gram stains, Hemolysis, motility, TSI)
Gram Negative Cocci (Gram Stains, oxidase, butyrate esterase)
Fastidious Gram Negative Rods (X & V Factors and Gram stains)
Fastidious Gram Negative Rods (X & V Factors and Gram stains)
Unknown # 1 Practical (GPC, GPR, GNC, Fast GNR)
Unknown # 1 Practical (GPC, GPR, GNC, Fast GNR)
Mardi Gras Break
Study Day
Enterobacteriaceae (Gram Stains, Urea, Citrate, ODC/LDC, PAD,
TSI, oxidase, IMViC, Motility, etc.)
Enterobacteriaceae (Gram Stains, Urea, Citrate, ODC/LDC, PAD,
TSI, oxidase, IMViC, Motility, etc.)
Unknown # 2 Practical (Enterobacteriaceae)
Unknown # 2 Practical (Enterobacteriaceae)
Unknown #3 Practical(mixed cultures)
Unknown # 3 Practical(mixed cultures)
Unknown # 4 Practical(mixed cultures)
Unknown # 4 Practical(mixed cultures)
Tu
Th
Tu
Th
Tu
Th
Tu
LSCLS Spring Meeting
Th
Tu
API Strips & Anaerobic ATP
Th
Susceptibility Testing
Tu
Spring Break
Th
Tu
Susceptibility Testing
Th
Kinyoun Stains
Tu
Final Practical (mixed cultures)
Th
Final Practical (mixed cultures)
*Must earn a 76% on all Practical Assignments to progress in course.
**The instructor reserves the right to adjust the schedule as needed.
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ULM School of Health Professions
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MLSC 3030 Medical Microbiology
Affective Evaluation
An affective evaluation shall determine 5% of the grade. The student begins with a
100% for the affective evaluation. 10 points are subtracted for each violation of proper
behavior.
Violations include, but are not limited to, the following:
1.
Wasting time or loitering in class or failure to participate
2.
Use of any personal electronic device including cell phones, I-Pods, radios, etc.
3.
Carelessness or inefficient work/school habits
4.
Creating or contributing to unsanitary or untidy conditions (includes failure to
keep classroom and laboratory tidy and orderly)
5.
Improper use or care of university property including laboratory equipment
6.
Distracting behavior during instructional activities including, but not limited to,
talking out of turn or passing notes
7.
Horseplay, practical jokes, etc.
8.
Unexcused Absence
9.
Tardiness
10.
Lack of respect for faculty and classmates through words, actions, or intent.
An affective score below 75% shall result in failure of the course.
Lasiter MLSC 3030:1701
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ULM School of Health Professions
Spring 2017
I verify that I have received a copy of the course syllabus for Medical Laboratory Science
3030, Medical Microbiology. This entire document has been reviewed with me by the
instructor of the class.
___________________________________
Student Name (PRINTED)
___________________________________
Student Signature
___________________________________
Date
___________________________________
Current (Local) Address
___________________________________
City/State/Zip
___________________________________
Current (Local) Phone Number
___________________________________
Email Address (PRINTED)
Lasiter MLSC 3030:1701
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