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Activity 5.1.4: Gram Staining Bacteria Aanna K. rhizophilia K. roseus S. aurantiaca Gram Positive Bacteria: (+) or (-) (-), with a little purple, bacilli (+), purple/black, coccus (+), purple/black, coccus (+), purple/black, coccus Gram Negative Bacteria: The purpose of the Gram staining lab is to distinguish between Gram positive and Gram negative groups. This is done by coloring the cells reddish pink or purple. When this is done a Gram positive bacteria retains the color purple due to the thicker layer of peptidoglycan in it's cell wall.