Download Cellular Respiration: the details

+
Cellular Respiration: the details
Miss Tee
+
1.
2.
3.
4.
+
Broken down… not so bad
1.
Glycolysis
 Cytosol (10 steps)
2.
Pyruvate oxidation
 Mitochondrial matrix (1 step)
3.
Kreb’s Cycle (TCA, citric acid cycle, CAC)
 Mitochondrial matrix (8 step cycle)
4.
Electron transport chain and chemiosmosis
 Inner mitochondrial matrix (IMM)
+
Energy Transfer
 1st
Law of Thermodynamics
 Chem potential energy
 Coupled reactions = efficiency
 Ultimate
goal: capture free energy in the form of _____
 Glycolysis
only gets energy production started
 2.1% of free energy to ATP
+
Mechanisms of Energy Transfer
Energy Transfer
Substrate-level
phosphorylation
-ADP + Pi = ATP
-Formed directly
Chemiosmosis
+
Terminology: MVPs
 Pi=
phosphate group
 ADP, ATP=
 NAD+=
 FAD=
adenose di/tri phosphate; currency
(nicotinamide adenine dinucleotide);
coenzyme used to shuttle e- to ETC
(flavin adenine dinucleotide); coenzyme and
e- carrier
+
Stage 1: Glycolysis
 Root
words?
 Def: glucose
molecule broken down into 2
pyruvate molecules; in cytoplasm
6
carbon sugar  3 carbon sugar + 3 carbon sugar
Glucose
+
Glycolysis
 Glucose
     G3P + G3P (precursors for step 2)
+
Glycolysis
 G3P
     Pyruvate + Pyruvate
+
Glycolysis: balance sheet

2 ATP used

4 ATP produced
2
ATP net
+
Another perspective…
+
Review of Mitochondria Structure

Specialize in production of
+++ ATP: “cell powerhouse”

IMM performs functions
associated with cell resp

Outer membrane

Inner membrane (IMM)

Cristae

Intermembrane space

Matrix
+
Stage 2: Pyruvate Oxidation
 Glycolysis
 2 Pyruvate
 Transported to IMM
+
Stage 2: Pyruvate Oxidation
1.
CO2 removed
2.
2-carbon oxidized by NAD+ (NAD+ now has potential
energy)
3.
CoA attached; molecule is prepped for next step
+
Stage 2: Pyruvate Oxidation
Ticket for next step
Pyruvate oxidation:
2 pyruvate + 2 NAD+ + 2 CoA  2 acetyl-CoA + 2 NADH +
2 H+ + 2 CO2
+
Summary
Acetyl-COA
+ Kreb’s Cycle (aka Tricarboxylic Acid
Cycle, TCA)
+