Download recognition sequence

Name_________________ Per_________
What is a restriction digest and what does it have to do with gel electrophoresis?
1) What does it mean to digest food?
2) What is an enzyme?
3) Where do restriction enzymes come from?
4) Why did bacteria evolve to produce restriction enzymes? Hint: it involves viruses.
5) To digest means to cut into smaller pieces. What do restriction enzymes digest?
6) EcoRI (say “Eco-Ar-One”) is the name for one kind of ____________
______________.
7) T/F: Restriction enzymes cut DNA in random places.
8) The name for the DNA sequence that a restriction enzyme cuts at is recognition
sequence. What is the recognition sequence for ECORI?
9) T/F: EcoRI and HindIII (say “Hine-Dee-Three”) have the same recognition sequences.
10)A student collects DNA samples from her three sisters. The student uses EcoRI and
HindIII to perform a restriction digest on each DNA sample. The student then runs the
digested samples on a gel. When she studies the gel, she notices that all three samples
produce a unique pattern of bands.
a) Gel electrophoresis separates chunks of DNA based on the __________ of the
chunks.
b) Why would the restriction digests produce different size chunks for each sister? Use
the term recognition sequence in your answer.
1) What does it mean to digest food?
To break it down into smaller pieces
2) What is an enzyme?
A protein that speeds up chemical reactions
3) Where do restriction enzymes come from?
Some kinds of bacteria
4) Why did bacteria evolve to produce restriction enzymes? Hint: it involves
viruses.
As a defense against viral infection. The bacteria’s restriction enzymes cut
up foreign DNA. The bacteria’s own DNA is protected by a coating.
5) To digest means to cut into smaller pieces. What do restriction enzymes
digest?
DNA
6) EcoRI (say “Eco-Ar-One”) is the name for one kind of _Restriction_
_Enzyme_.
7) T/F: Restriction enzymes cut DNA in random places.
8) The name for the DNA sequence that a restriction enzyme cuts at is
recognition sequence. What is the recognition sequence for ECORI?
GAATTC
9) T/F: EcoRI and HindIII (say “Hine-Dee-Three”) have the same recognition
sequences.
10)
A student collects DNA samples from her three sisters. The student
uses EcoRI and HindIII to perform a restriction digest on each DNA sample.
The student then runs the digested samples on a gel. When she studies the
gel, she notices that all three samples produce a unique pattern of bands.
a) Gel electrophoresis separates chunks of DNA based on the _length_ of the
chunks.
b) Why would the restriction digests produce different size chunks for each
sister? Use the term recognition sequence in your answer.
Each sister has a unique sequence of DNA nucleotides. Therefore, each
sister will have a unique number of recognition sequences for EcoRI and
HindIII. After the restriction digest, the number of DNA chunks and the
size of the DNA chunks will be different for each sister.