Download Outline --- Programmed Cell Death 1. Apoptosis An overview: the

Outline --- Programmed Cell Death
1. Apoptosis
 An overview: the establishment of the concept/field
Morphological observations (concept/hypothesis)
Medicine/cancer (Bcl-2, the founder of a new class of oncogene)
Basic research (proof of the concept by C. elegans genetics)
 In-depth discussion of certain topics
Mitochondria-mediated caspase activation
(Bcl-2 family; caspases; Apaf-1; IAPs and Smac; other regulatory mechanisms)
(Point of no-return)
Crosstalk of the extrinsic pathway with the intrinsic pathway
Events after caspase activation
 Unanswered questions
 Some new directions
 Methods for measuring apoptosis
Principle underlying each method
Pros and cons of each method
 Cell death and cancer biology
Oncogene-induced apoptosis
Oncogene addiction
Targeting apoptosis directly
2. Non-apoptotic, programmed cell death
 Conceptually what is the qualification for a programmed but non-apoptotic cell death?
 Pathways for programmed necrosis, if any
 Autophagic cell death?
3. Autophagy
 Mechanism
Nutrient sensing kinase mTOR; ATG1/ULK1 kinase complex; ubiquitination-like
reactions
Cargo-specific autophagy (e.g., mitophagy)
 Biological functions
 Role in cell death
 Cancer/disease relevance
Paper to discuss:
Glutaminolysis and Transferrin Regulate Ferroptosis.
Gao M, Monian P, Quadri N, Ramasamy R, Jiang X.
Mol Cell. 2015 Jul 16;59(2):298-308. doi: 10.1016/j.molcel.2015.06.011. Epub 2015 Jul 9.
PMID: 26166707
Review paper:
Regulated necrosis: disease relevance and therapeutic opportunities.
Conrad M, Angeli JP, Vandenabeele P, Stockwell BR.
Nat Rev Drug Discov. 2016 Jan 18. doi: 10.1038/nrd.2015.6. [Epub ahead of print]
PMID: 26775689