Download PE anti-mouse Ly6K Antibody

Version: 1
Revision Date: 04/28/2016
PE anti-mouse Ly6K Antibody
Catalog# / Size
151304 / 100 µg
151303 / 25 µg
Clone
MK34 (See other available formats)
Other Names
Lymphocyte antigen 6K, Ly-6K, mLy-6K
Isotype
Rat IgG2a, κ
Ave. Rating
★★★★★ 0 reviews
Description
Lymphocyte antigen 6K (Ly6K) is a 17 kD GPI-anchored membrane protein
expressed by testicular germ cells. Ly6K associates with TEX101 and may
have a role in spermatogenesis. It is also expressed in some cancer cell
types; a recent study also demonstrated its expression in plasma cells. A
soluble 12 kD form of Ly6K has been reported.
Ly6K transfected-HEK-293 cells were stained
with clone MK34 (filled histogram) PE or rat
IgG2a, κ PE isotype control (open histogram).
See PE spectral data
Reactivity
Mouse
Immunogen
Mouse testicular homogenate.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal
conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from
prolonged exposure to light. Do not freeze.
Application
FC - Quality tested
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow
cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per
million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal
performance for each application.
Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemical
staining on frozen tissue sections1, western blotting1, and immunoprecipitation 1.
Application References
1. Maruyama M, et al. 2010. Biochem. Biophys. Res. Commun. 402:75. (IHC-F, IP, WB)
(PubMed link indicates
BioLegend citation)
Publication Library
RRID
AB_2616815 (BioLegend Cat. No. 151304)
AB_2616814 (BioLegend Cat. No. 151303)
Antigen Details
Structure
GPI-anchored membrane protein of 17 kD, soluble protein of 12 kD.
Distribution
Testicular germ cells, some cancer cell types, and plasma cells.
Function
Possible role in spermatogenesis.
Interaction
TEX101.
Antigen References
1. Fujihara Y, et al. 2014. Biol. Reprod. 90:60.
2. von Allmen CE, et al. 2008. Mol. Immunol. 45:2727.
3. Yoshitake H, et al. 2008. Biochem. Biophys. Res. Commun. 372:277.
4. de Nooij-van Dalen AG, et al. 2003. Int. J. Cancer 103:768.
Gene ID
76486
UniProt
View information about Ly6K on UniProt.org
Documentation
Technical data sheet
Certificate of Analysis
Safety Data Sheet
Related Protocols
Cell Surface Immunofluorescence Staining Protocol
Related FAQs
There are no FAQs for this product.
Related Categories
Antibodies and More > Cell Biology > Cancer Biomarkers > Ly6K >
Immunology > Mouse Immunology > CD and Related Molecules > Ly6K > MK34 >
Immunology > Mouse Immunology > B Cells > Ly6K >
Other Formats
Description
Clone
Applications
Purified anti-mouse Ly6K
MK34
FC, IHC-P, IHC-F, IP, WB
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