Download Genetics Laboratory (BIOL 311L)

Genetics Laboratory (BIOL 311L)
Course description: Laboratory experience to illustrate and explain the principles covered in
BIOL 311. Prerequisite or co-requisite: BIOL 311. One semester; one credit.
DATE
Week 1
Assigned reading/resource
TOPIC/TASK
Review of the syllabus
Lab rules, p.1
http://www.phys.ksu.edu/gene/a1.html
Yeast simple cross, pp. 2-7
(will come into lab twice briefly during the week)
Introduction to S.
cerevisiae, cross
Media preparation (YED,
MIN, YEKAC)
Finish Yeast simple cross, p. 8
Finish observation of yeast
life cycle, 2 x 2 segregation
Introduce mapping
Week 2
Brief intro into plasmids/restriction enzymes, p. 9
http://www.carolina.com/pdf/activities-articles/plasmidmapping-exercises-2008.pdf
MOVIE: Cracking Your Genetic Code
Restriction mapping exercise, pp. 10-12
Week 3
Digest plasmid DNA with four
different enzymes, run gels
Chromosome mapping (lecture support)
Continue restriction mapping exercise, pp. 9-14
Do double digests of
plasmid DNA, run gels
E. coli/responsible handling of E. coli, pp. 15-16
Sterile technique, serial dilutions, pp. 17-19
Begin preparation for materials needed in Ames Test
Lab report of restriction mapping exercise due
Begin work with bacterial
cultures
Prepare media
Ames Test: pp. 20-29
(come in next day to remove serial dilution plates from
incubator; remove assay plates after 48 hours and choose
one colony for further study)
Serial dilution
The assay with suspected
mutagens
Polymerase Chain Reaction, p. 30-31
Revertion--Phenotype to DNA, pp. 32–37
Counting CFUs
DNA isolation
PCR of mutant DNA
Week 4
Week 5
Week 6
Week 7
Week 8
Week 9
MIDTERM LAB
EXAM/PRACTICAL
FALL BREAK!
Compare sequences of Ames Test revertants
Week 10
http://www.silencinggenomes.org/
Return to culturing E. coli, pp. 40-41
C. elegans, p. 42
Week 11
http://www.wormclassroom.org/short-history-c-elegansresearch
Observing wild-type and mutant C. elegans, pp. 43-48
Culturing C. elegans, pp. 49-52
Compose list of
carcinogens and mutations
created
Seed bacteria
Prepare media
Observing/chunking/
picking C. elegans
Week 12
RNA interference, p. 54-58
Inducing RNAi by feeding, pp. 59-68
http://www.pbs.org/wgbh/nova/sciencenow/3210/02.html
Seeding, chunking, and
picking worms
Bioinformatics exercise
Inducing RNAi by feeding, p. 67-68
Finish gathering of RNA
silencing results
DNA extraction,
amplification of PTC gene
locus
Week 13
Analysis of the human PTC locus, pp. 69-70
http://www.dnalc.org/resources/animations/index.html
DNA digestion with Fnu4HI, p. 70
Week 14
Fingerprint Ridge Counts, pp. 72-77
http://www.migeneticsconnection.org/genomics/
Mulitfactorial Traits/Fingerprint Ridge Count.htm
Gel electrophoresis of amplified PTC DNA, p. 71
Week 15
Population genetics, p. 78-80
Film: Genetics of Bitter Taste Perception
http://www.youtube.com/watch?v=PGoBr0usBzA
Week 16
Restriction digest of PCR
fragments
Prepare own fingerprints,
calculate TRC (10 pt. data
sheets for turn-in)
Run PCR/digested DNA on
gel
PTC tasting
FINAL LAB
EXAM/PRACTICAL