Download MicroRNA-6165 Down-regulates IGF1R and Enhances Apoptosis in

MicroRNA-6165 Down-regulates IGF1R and Enhances
Apoptosis in SW480 Cell line
Maryam Hassanlou1, Bahram M. Soltani1* and Seyed J. Mowla1
1
Department of Biology, Tarbiat Modares University, Tehran, Iran.
[email protected] * Tarbiat Modares University
MicroRNAs are long non-coding small RNAs that regulate post-transcriptional mRNA expression.
Numerous evidences have shown the important roles for these regulators in cellular proliferation,
differentiation and apoptosis. MicroRNAs expression have shown in several human
developmental process including differentiation of stem cells to neuronal cells. Neurotrophins
(NT) are a family of secretive growth factors that do their functions via binding to their specific
receptors (TrkA, TrkB and TrkC) or their common receptor (p75ntr). P75ntr is the common
receptors of NTs that binds to all members of NTs family and has important roles in survival,
differentiation and proliferation of several types of cells. Recently, a MicroRNA named hsa-mir6165 has been discovered in forth intron of p75ntr which the preliminary lab studies have shown
its involvement in apoptosis induction in some human cell lines. The bioinformatics analysis has
revealed the IGF1R as its potential target genes. Considering the revealed evidence of association
of this MicroRNA host gene (p75ntr) as facilitator factor for apoptosis during development and
after injury, we analyzed the expression of hsa-mir-6165 in SW480 cell lines. Our results indicated
that hsa-mir-6165 not only can down-regulate the expression of IGF1R but although significantly
can enhance the apoptosis rate in this cells.