Download Product Sheet - Life and Soft

The first in-house CRISPR
application to design both
gRNA and donor template
sequences required for
genome editing.
is a
complete and fully
customizable tool to
facilitate and optimize
your CRISPR experiments.
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The introduction of targeted genomic sequences changes by CRISPR
technology into living cells is becoming a powerful tool for gene therapy
or disease modelling. CRISPR only requires a nuclease and customized
nucleic sequences. Preliminary bioinformatics analysis for both gRNA
design and donor template can improve the success of the experiment.
This is where the CRISPR LifePipe will make genome editing as simple
as using a text editor.
DESIGN OF RNA GUIDE (OR gRNA)..
The RNA guide, or gRNA, is a short RNA sequence which guides the Cas9 endonuclease
to the targeted region to cut on the genome. gRNA is crucial for CRISPR gene editing
because it provides targeting efficiency on the region of interest on the genome while
limiting the non-specific off-targets.
Our gRNA design tool is built to allow you to target different genomic regions:
• Targeting a region present on most of the transcripts of a gene
• Targeting an exon or an intron of a transcript
• Targeting UTR region of a gene
• Targeting a particular amino acid
CRISPR technology is a genome engineering
technology which allows accurate genome
modification of a cell.
It is based on the immune system
CRISPR/Cas9 discovered in bacteria.
Cho et al. Nat Biotech 2013
Our tool selects gRNA sequences with the lowest number of off-targets to avoid side
effects within the cell. It also designs efficient gRNA estimating the gRNA secondary
structures and calculating the number of SNPs on its sequence.
Advanced options provide a precise configuration to optimize the design of gRNA.
Indeed the tool offers different specific designs parameters like the GC percent of the
sequence, the presence of TLR motifs or the presence of nucleodide stretches on the
RNA sequence.
Cong et al. Science 2013
DESIGN OF DONOR TEMPLATE..
CRISPR technology is able to modify specific
DNA sequence using a RNA guide (gRNA).
This short RNA sequence guides the Cas9
endonuclease to the genomic region to target
and cut. A donor template sequence uses
DNA repair mecanism of the cell to allow
modifications of DNA sequence.
The donor template is a DNA sequence inserted into the cell along with the gRNA and
the Cas9 endonuclease. It is acting by homologous recombination during DNA repair
mecanism to replace DNA sequence of the cell by the modified DNA sequence of the
donor template.
• Insertion of a mutation into a gene
• Gene tagging in 5’ or 3’
“
CRISPR: GENE EDITING IS JUST
THE BEGINNING
The real power of the biological tool lies
in exploring how genomes work”
Nature, 2016
• Gene Knock-Out
Our tool can design plasmid donor sequences with variable lengths of the two homology
arms and different mutations or tag to insert into the cell, or ssDNA oligo (ssODN).
An option allows the insertion of a particular DNA fragment between the two homology
arms, like a selection cassette.
The gRNA is always inactivated by our tool to prevent the Cas9 to cut the donor sequence.
A quality control of the donor template is also systematically performed.
The application returns a graphical output to visualize the full donor template sequence,
the position of the gRNA sequence and the inserted variations on the donor.
WHY CHOOSE CRISPR LIFEPIPE?..
The CRISPR tools have been developped by Life&soft team to meet all the needs of the
CRISPR users. These tools will facilitate and improve all steps required for a high quality
and successful CRISPR experiment preparation.
Convenient
Our application gather two different tools together required for
CRISPR genome editing, from gRNA design to the choice of donor
template sequence
Complete
Our tool has been developped considering the new findings and
hints published into scientific literature.
Supported
Both tools are updated and supported by our team regularly.
Flexible
Easy to use
All our CRISPR tools are entirely customizable regarding your specific
and evolving needs : species to study, PAM and Cas9 used…
Intuitive web interface fully customizable by user regarding his
specifc needs.
Currently available on BIOVIA Pipeline Pilot®. The portability of the
Easy to deploy application out-of-the-box on linux environment is being developped.
CONTACT US..
Les Colocs (Parc Noveos)
8 B avenue Descartes - 92350 Le Plessis-Robinson - France
[email protected]
+33 (0) 6 19 53 89 27
www.lifeandsoft.com
www.chrysalis-lamarque.fr - Photos & graphiques : Shutterstock.com - Fotolia.com - Life&Soft - Servier Medical Art de Servier est mis à disposition selon les termes de la licence Creative Commons Attribution 3.0 France
Different donor sequences can be designed with our tool regarding the modification that
is expected in the cell: