Download End Semester Examination (July- Dec, 2015) BT 305: Enzyme

B.Tech./201S/ES/BIO-30S/2/4
NATIONAL INSTITUTE OF TECHNOLOGY
(Established
by Ministry
of Human Resource Development,
Yupia, District- Papum Pare, Arunachal
Fax: 0360-2284972.
Govt. Of India)
Pradesh- 791112
Email: [email protected]
End Semester Examination (July- Dec, 2015)
BT 305: Enzyme Engineering
Full Marks: 100
Time: 3 Hr
Instructions:
1. Answer as many questions as you can but maximum score will
be limited to 100.
2. Please read the instruction carefully
(Vmax= Maximum velocity; Km=Michaelis constant; [s] =
Substrate; Ve =Velocity)
UNIT-I
(i)
Answer all questions
l.(a) How sulfa drug acts as a competitive inhibitor? Prove
~
2
(b)What is active site?
1
(c) How aspartyl protease can be use for HIV prevention? 2
2. Short note (ANY ONE)
(a)
Lyophilization
(b)
Enzyme immune-biosensor
(ii) Answer any ONE question
(5x1=5)
(15x1=15)
1. (a) Mention enzyme specificity.
6
(b) Describe that free energy is a useful thermodynamics
function for understanding enzyme.
5
(c) Explain Lineweaver - Bruk equation.
4
2. (a) Explain what are cofactors and coenzymes?
3
(b) Explain enzyme inhibition with all reactions and
double reciprocal plots.
10
(c) What are the factors get influence the rate of enzyme
action?
2
UNIT-II
(i)
1.
Answer all questions
A solution contains initially 35.0xl0-4 moll," of peptide
substrate and1.50ig chymotrypsin in 2.5rnL volume.
After 10 minutes, 18.6xl0-4 moll," of peptide substrates
remain. Molar mass of chymotrypsin is 45,000 g mol".
Calculate rate of reaction, enzyme activity and specific
activity and turn over number.
5
2.
An enzyme-catalyzed reaction has a Km of ImM and
a Vmax of 5nM· S-I. What the reaction velocity when
the substrate concentration
is (a) 0.35mM (b) 1.6mM.
(Note: when units in the numerators
and denominator
cancel)
(ii)Write short notes (ANY THREE)
(a)
Spectrophotometer
(b)
Chromatographic
assay
(3+2)
(5x3=15)
(c) Gel electrophoresis
(d) Ultra centrifugation
(e) Ultra filtration
UNIT III
(i) Answer all questions
(a) Explain catabolism and anabolism with an example. 2
(b) At the time of enzyme isolation, cell lysis methods are
required. Explain briefly about cell lysis methods.
8
(ii) Answer any ONE question
1. (a) Prove that
V. -
(15xl=15)
7
Vmax[S]
Km+[S]
o (b) Mention what are the working principals of biosensor?
7
(c) Explain "all enzymes are protein but all proteins are not
enzyme".
1
2. (a) Briefly describe enzyme substrate interaction.
5
(b) Described how different enzymes are classified and
named.
8
(c) Prove that Km=[S] When Vo= 1/2Vmax
2
(i)
UNIT IV
Answer all questions
1. What are the difference between NMR and X-ray
crystallography?
10
(ii) Answer any ONE question
1. Short notes (ANY THREE)
(5X3=15)
Application of enzymes in
(a)
(b)
(c)
(d)
(e)
Biomedical industries
Food industries
Brewing industries
Dairy and paper industries
Drug industries
2. Briefly explain what are the enzyme based biosensors
has been used for health care.
15